• The Plant Pathology Journal
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• v.31 no.3
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• pp.219-225
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• 2015

The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a set of loop primers since one out of six primers showed PVX specificity. Based on real-time monitoring, RT-LAMP detected PVX around 30 min, compared to 120 min for RT-PCR. By adding a fluorescent reagent during the reaction, the extra step of visualization by gel electrophoresis was not necessary. RT-LAMP was conducted using simple inexpensive instruments and a regular incubator to evaluate whether RNA could be amplified at a constant temperature instead of using an expensive thermal cycler. This study shows the potential of RT-LAMP for the diagnosis of viral diseases and PVX epidemiology because of its simplicity and rapidness compared to RT-PCR.

• Physical Therapy Rehabilitation Science
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• v.4 no.2
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• pp.94-102
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• 2015

Objective: The purpose of this study is to investigate the effect of treadmill gait training with patellar taping on gait abilities in chronic stroke patients. Design: Randomized controlled trial. Methods: Thirty chronic stroke patients who have been diagnosed at least six months or before were recruited in Gyeongin Rehabilitation Center Hospital, located in Incheon. Patients who were enrolled in this study were randomized to experimental group (n=15) or control group (n=15). Treadmill with patella taping training group patients were applied with patellar taping when they were being trained on a treadmill. Control group patients were being trained on a treadmill without any kind of taping. Gait parameters were measured with a GAITRite$^{(R)}$ system which evaluated gait performances. Gait trainings were done for 30 min/day, 5 days/week, for 4 weeks. Results: After treadmill training, treadmill with patella taping training group showed a significant improvement in gait abilities, including velocity, cadence, paretic and non-paretic step length, and double support period (p<0.05). However, in general treadmill group, there were no significant differences in gait parameters except velocity and cadence. There was a significant difference in gait performance in the experimental group compared with the control group, except for the gait symmetry ratio (p<0.05). Conclusions: According to this result of this study, it seems that application of patellar taping in treadmill gait training for chronic stroke patients significantly improved gait abilities of these patients. Also, we can conclude that patella taping is thought to be useful in real clinical settings where there are many chronic patients who are in need of improvement in their gait abilities.

• The Korea Journal of Herbology
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• v.30 no.3
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• pp.19-24
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• 2015

Objectives : HPL-1, a novel herbal medicine which is composed of five herbs such as Kalopanacis Cortex, Chaenomelis Fructus, Raphani Semen, Atractylodis Rhizoma and Pulvis Aconiti Tuberis Purificatum, was developed for treatment of osteoarthritis. This study is aimed to develop analytical method for consistent quality control of HPL-1 and validate chromatographic method. Methods : Chromatographic analysis was performed using ultra-high performance liquid chromatography - diode array detector (UHPLC-DAD) equipped with RP-amide column, column oven, and auto sampler. Marker compounds [protocatechuic acid, chlorogenic acid, liriodendrin, 3,5-dicaffeoylquinic acid, ${\beta}$-D-(3-O-sinapoyl)-fructofuranosyl-$\alpha$-D-(6-O-sinapoyl)glucopyranoside and benzoylmesaconine] were separated by step gradient elution of acetonitrile and 0.1% phosphoric acid/water. The method validation was evaluated by quantitative validation parameters of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to KFDA guideline.Results : An optimized method for six marker compounds in HPL-1 was established by UHPLC-DAD. The correlation coefficient (R²) with each calibration curve was greater than 0.99. The LOD and LOQ were within the range of 0.008-0.090 and $0.023-0.274{\mu}g/mL$, respectively. The relative standard deviation (RSD) of intra- and inter-day variability were less than 4.0%. The result of recovery test was range from 93.3-106.3% with RSD < 4.0%.Conclusions : These results suggest that the quantitative UHPLC method is precise, accurate, effective for quality evaluation of HPL-1. The method may also contribute to improve quality of crude drug preparations used for treatment of various diseases.

• The Journal of the Korean dental association
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• v.48 no.11
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• pp.829-840
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• 2010

In recent years, medical techniques have provided patients with various measures to improve their quality of life. For dental treatment, drug-mediated sedation techniques for relieving dental anxiety have been developed, but behavior control through drugmediation may be limited because of possible side effects, contraindications, and the additional expense to the patient. Many patients tend to avoid the treatment or are unwilling to accept it and this makes both patients and dentists feel pressured. The field of NLP application might alleviate this uncomfortableness. Recently, NLP has spread to the dental and medical field rapidly and has been used in surgical treatments as well as in direct psychotherapy. NLP techniques which could be applied to dental phobic patients are as follows. 1) anchoring, 2) dissociation, 3) submodality change, 4) time line threapy, 5) swish pattern, 6) six step reframing, 7) parts integration, 8) modeling and imagination and so on. The aim of this study is to examine the strategy of NLP psychology so that dental phobic patients can be treated efficiently and effectively by the application of behavior management. Through NLP, patients can be induced to have more positive attitudes and experiences in future dental treatment.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.3
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• pp.308-313
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• 2006

The extent of impact dispersion is a function of parameters including gun geometry and tolerances, the foe control system, projectile manufacturing tolerances, etc. The study here investigates potential impact point accuracy improvement for a projectile realized by replacing the rigid nose cone wind screen with a passive nose. Toward this end, a nose projectile dynamic model is derived which consists of the standard six degrees of freedom similar to a rigid projectile plus three additional degrees of freedom associated with rotation of the nose with respect to the main projectile body. By Observing the pitch and yaw movement of the nose in the simulation results, it is believed to be possible to reduce the effects of uncertainties which is occurred at firing step.

• IE interfaces
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• v.25 no.4
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• pp.393-404
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• 2012

Increasing safety requirements of automobile are asking companies to find out solutions, based on the ISO 26262 which is a functional safety standard. ISO 26262 is an adaptation of the IEC 61508 for automotive electric/electronic systems. ISO 26262 provides a V model for ECU (Electronic Control Unit) development process to secure safety against vehicle. It well describes the requirements, necessary works and their resulting products for each development phase. However, it is difficult to apply to product development for achieving functional safety in the electric/electronic systems of an automobile because it lacks explanation on the working steps to follow and the methodologies and tools to be used in each step. In this paper, we introduce the outline of the ISO 26262 product development process and present a DFSS (Design For Six Sigma) road-map based on the ISO 26262 product development process as a way to operate efficiently the ISO 26262 product development process. The DFSS road-map consists of five phases: Define, Measure, Analyze, Design, and Verify. The detailed activities, tools, inputs, and work products are given for each phase.

• Journal of Power Electronics
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• v.14 no.6
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• pp.1166-1177
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• 2014

A new type of topology with medium-frequency-transformer (MFT) isolation for medium voltage wind power generation systems is proposed in this paper. This type of converter is a high density power conversion system, with high performance features suitable for next generation wind power systems in either on-shore or off-shore applications. The proposed topology employs single-phase cascaded multi-level AC-AC converters on the grid side and three phase matrix converters on the generator side, which are interfaced by medium frequency transformers. This avoids DC-Link electrolytic capacitors and/or resonant L-C components in the power flow path thereby improving the power density and system reliability. Several configurations are given to fit different applications. The modulation and control strategy has been detailed. As two important part of the whole system, a novel single phase AC-AC converter topology with its reliable six-step switching technique and a novel symmetrical 11-segment modulation strategy for two stage matrix converter (TSMC) is proposed at the special situation of medium frequency chopping. The validity of the proposed concept has been verified by simulation results and experiment waveforms from a scaled down laboratory prototype.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.10
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• pp.186-192
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• 1998

This paper presents a high performance low switching PWM technique or the propulsion system of railway such as subway and high speed train. In order to achieve the continuous voltage control to six-step and s low harmonics with low switching frequency under 500Hz, the synchronous technique is combined with a space vector overmodulation and implemented by using DSP. Improved performance and a validation of proposed method are showed by the digital simulation and the experimental results using a 1.65MVA IGBT VVVF inverter and inertia load equivalent to 160 tons railway cars.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.10
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• pp.1930-1935
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• 2009

This paper deals with the torque ripple reduction of permanent magnet synchronous motor using harmonic current injection. Torque ripple of electric motor reduces system stability and performances, therefore efforts to reduce torque ripple are exerted in the design process. Torque ripple can be reduced by appropriate pole/slot combination, skew of rotor or stator, design of magnetic circuit, etc. In addition, torque ripple can be also reduced by input voltage and current, and many researches have been conducted to reduce torque ripple for six-step drive. Torque ripple reduction for current vector controlled permanent magnet synchronous motor also have been conducted and verified by investigating back emf wave form. Torque ripple reduction in this paper started from getting torque profile according to input current and electrical angle calculated by FEA, then instantaneous currents at each electrical angles for constant torque are calculated and applied to experiments. Therefore, 0% of torque ripple can be obtained theoretically with harmonic current injection. In order to maximize the effect of torque ripple reduction, a BLDC motor having high harmonic component of back emf is chosen. With sinusoidal current drive, over 100% of torque ripple is obtained initially, then 0.5 % of torque ripple is obtained by FEA using harmonic current injection. The effect is verified by experiment and the presented method can be effectively applicable to Electric Power Steering(EPS).

• Restorative Dentistry and Endodontics
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• v.24 no.1
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• pp.55-66
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• 1999

The purpose of this study was to evaluate the microleakage of 5 current dentin bonding systems which are composed of 2 multi-bottle systems(Scotchbond Multi-Purpose, All Bond2) and 3 one-bottle systems(Single bond, One-Step, Prime & Bond). In this in vitro study, class V cavities were prepared on buccal and lingual surfaces of sixty extracted human premolars and molars on cementum margin. The experimental teeth were randomly divided into six groups of 10 samples (20 surfaces) each, Group 1 : Scotchbond Multi-Purpose ; Group 2 : All Bond 2 ; Group 3 : Single Bond ; Group 4 : One-Step ; Group 5 : Prime & Bond ; Group 6 : no bonding agent(control). The bonding agent and composite resin were applied for each group following the manufacturer's instructions. After 500 thermocycling between $5^{\circ}C$ and $55^{\circ}C$, the 60 teeth were placed in 2% Methylene blue dye for 24 hours, then rinsed with tab water. The specimen were embedded in clear resin, then sectioned buccolingually through the center of restoration with a low speed diamond saw. The dye penetration on each of the specimen were then observed with a stereomicroscope at ${\times}20$. The results of study were statistically analyzed using the Student-Newmann-Keul's Methods and the Mann-Whitney Rank Sum Test. The resin/dentin interfaces were examined under Scanning Electron Microscopy. The results of this study were as follows. 1. None of the dentin bonding systems used in this study showed significant difference in leakage values at both the enamel and the dentin margins (P>0.05). 2. In all groups except the control, leakage value seen at the enamel margin was significantly lower than that seen at the dentin margin (P<0.05). 3. Compared to the control group, all the groups treated with dentin bonding systems showed significantly lower leakage value at both enamel and dentin margins (P<0.05). 4. In the SEM view, gaps were observed in the composite resin / dentin interface in group 6 where no dentin bonding agent was used, and in all the other groups (group 1, 2, 3, 4, 5) composite resin, hybrid layer, and dentin were seen to be closely adhering to each other where there were no leakages. Well-developed resin tags 3~100${\mu}m$ in length infiltrated dentinal tubules past the hybrid layer and a hybrid layer 1~5${\mu}m$ thick had developed between the dentinal surface and the composite resin surface.