• BMB Reports
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• 제31권1호
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• pp.58-63
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• 1998

In this study, a mouse monoclonal antibody (mAb) against gp41(584-618), the immunodominant epitope protein, was generated. For this purpose, BALB/c mice were immunized with double branched multiple antigenic peptides derived from the HIV-1 gp41(584-618) sequence, and antibody-secreting hybridoma were produced by fusion of mice splenocytes with SP2/0 myeloma cells. One clone producing an antigen specific mAb, termed KI-41(isotype IgG1) was identified, whose specific reactivity against gp41(584-618) could be confirmed by ELISA and Western blot analysis. Epitope mapping revealed the recognition site of the mAb KI-41 to be located around the sequence RILAVERYLKDQQLLG, which comprises the N-terminal region within the immunized gp41(584-618) peptied. Since this mAb recognizes this specific epitope within the HIV-1 gp41 without any cross-reactivity to other immunodominant regions in the HIV-2 gp35, KI-41 will provide some alternative possibilities in further applications such as the development of indirect or competitive ELISA for specific antibody detection in HIV-1 infection or for other basic researches regarding the role and function of HIV-1 gp41.

• 한국환경복원기술학회지
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• 제2권1호
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• pp.48-57
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• 1999

This study was aimed to establish biotop unit of the mixed rural city for the method and process of the biotop mapping system. Survey site was Maejuri of Seunghwan(158ha), Gisanri of Mokchon(132ha) and Namkwanri, Pungsemeon(214ha). So the main process was divided by 4 schemes such as Biosphere, Geosphere, Antrosphere and Evaluation. Also the GIS(geographic information system) was used to make the database of the biotop and biotop complex, analyze the cross-combinations and analyze the characters of the biotop. Biotop mapping system had 5 steps which were proceeded with research goals, constructing the spatial database and attribute database, classifying the 3 types of biotop such as tree/shrub biotop, grass biotop and wetland biotop, cross-analyzing 3 biotop types with land use, habitat characters, relief characters and danger/disturbance elements and evaluating the 3 types of biotop. The results of applicating the biotop mapping system on the research site as followings : The distributions of the land uses in Maejuri, Seunghwan eup in Chonan city were recorded by forest(29.8%), orchard(14.1%) and landscaping around building site(9.0%). Gisanri, Mokchonmeon were composed of forest(64.5%), farm(12.8%) and Namkwanri, Pungsemeon were concentrated rice field(39.6%), dwell district(22.4%). The Tree/Shrub biotop type was reclassed by the forest type, natural and artificial decidous type with natural coniferous. The Grass biotop type was reclassed by the wild grassland type, garden type and peddy field with wild grassland. The distributions of the wet land were pointed high at the wet land type with reed marsh and edge vegetation around wet land in reservoir and river. The evaluation of the mapped bitopes was completed to the following aspect, "amenity" and "environmental education". A high value of 7.13%(1 class) was shown Maejuri, Seunghwan eup. The regions which were studied synthetically are divided to three parts ; the area where have nature and art mixed(Seunghwan), the area which is more artificial because people inhabit there for a long time(Pungsemeon) and the area that ecological environment is threatened by development pressure(Mokchonmeon). Therefore, ecological restoration plan which depends on specific property of the regions should be established. Also the interdisplinary researches were needed to develop the BMS(Biotop Mapping System) in Korea because of the differences with Germany, England's ecological habitat conditions.

• Journal of Microbiology
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• 제41권4호
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• pp.340-344
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• 2003

The group I intron from Tetrahymena thermophila has been demonstrated to employ splicing reactions with its substrate RNA in the trans configuration. Moreover, we have recently shown that the transsplicing group I ribozyme can replace HCV-specific transcripts with a new RNA that exerts anti-viral activity. In this study, we explored the potential use of RNA replacement for cancer treatment by developing trans-splicing group I ribozymes, which could replace tumor-associated RNAs with the RNA sequence attached to the 3' end of the ribozymes. Thymidine phosphorylase (TP) RNA was chosen as a target RNA because it is known as a valid cancer prognostic factor. By performing an RNA mapping strategy that is based on a trans-splicing ribozyme library, we first determined which regions of the TP RNA are accessible to ribozymes, and found that the leader sequences upstream of the AUG start codon appeared to be particularly accessible. Next, we assessed the ribozyme activities by comparing trans-splicing activities of several ribozymes that targeted different regions of the TP RNA. This assessment was performed to verify if the target site predicted to be accessible is truly the most accessible. The ribozyme that could target the most accessible site, identified by mapping studies, was the most active with high fidelity in vitro. Moreover, the specific trans-splicing ribozyme reacted with and altered the TP transcripts by transferring an intended 3' exon tag sequence onto the targeted TP RNA in mammalian cells with high fidelity. These results suggest that the Tetrahymena ribozyme can be utilized to replace TP RNAs in tumors with a new RNA harboring anti-cancer activity, which would revert the malignant phenotype.

• 한국지하수토양환경학회지:지하수토양환경
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• 제16권6호
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• pp.66-78
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• 2011

It is getting difficult to manage water resources in South Korea because more than half of annual precipitation is concentrated in the summer season and its intensity is increasing due to global warming and climate change. Artificial recharge schemes such as well recharge of surface water and roof-top rainwater harvesting can be a useful method to manage water resources in Korea. In this study, potential artificial recharge site is evaluated using geographic information system with hydrogeological and social factors. The hydrogeological factors include annual precipitation, geological classification based on geological map, specific capacity and depth to water level of national groundwater monitoring wells. These factors were selected to evaluate potential artificial recharge site because annual precipitation is closely related to source water availability for artificial recharge, geological features and specific capacity are related to injection capacity and depth to water is related to storage capacity of the subsurface medium. In addition to those hydrogeological factors, social aspect was taken into consideration by selecting the areas that is not serviced by national water works and have been suffered from drought. These factors are graded into five rates and integrated together in the GIS system resulting in spatial distribution of artificial recharge potential. Cheongsong, Yeongdeok in Gyeongsangbuk-do and Hadong in Gyeongsangnam-do, and Suncheon in Jeollanam-do were proven as favorable areas for applying artificial recharge schemes. Although the potential map for artificial recharge in South Korea developed in this study need to be improved by using other scientific factors such as evaporation and topographical features, and other social factors such as water-curtain cultivation area, hot spring resorts and industrial area where groundwater level is severely lowered, it can be used in a rough site-selection, preliminary and/or feasibility study for artificial recharge.

• Geomechanics and Engineering
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• 제34권1호
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• pp.29-41
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• 2023

The 3D geospatial modeling of geotechnical information can aid in understanding the geotechnical characteristic values of the continuous subsurface at construction sites. In this study, a geostatistical optimization model for the three-dimensional (3D) mapping of subsurface stratification and the SPT-N value based on a trial-and-error rule was developed and applied to a dam emergency spillway site in South Korea. Geospatial database development for a geotechnical investigation, reconstitution of the target grid volume, and detection of outliers in the borehole dataset were implemented prior to the 3D modeling. For the site-specific subsurface stratification of the engineering geo-layer, we developed an integration method for the borehole and geophysical survey datasets based on the geostatistical optimization procedure of ordinary kriging and sequential Gaussian simulation (SGS) by comparing their cross-validation-based prediction residuals. We also developed an optimization technique based on SGS for estimating the 3D geometry of the SPT-N value. This method involves quantitatively testing the reliability of SGS and selecting the realizations with a high estimation accuracy. Boring tests were performed for validation, and the proposed method yielded more accurate prediction results and reproduced the spatial distribution of geotechnical information more effectively than the conventional geostatistical approach.

• 대한핵의학회지
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• 제38권2호
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• pp.161-170
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• 2004

분자영상은 살아있는 개체의 몸 속에서 일어나는 생물학적 반응이나 특정한 표적분자를 비관혈적이며 반복적으로 영상화하는 기술이다. 이를 위해서는 두 가지 기본 요소가 요구되는 바 하나는 관심 생물현상에 의해 농도나 분광특성이 변하는 분자영상용 추적자이며 다른 하나는 이런 추적자를 모니터링하는 장비이다. 분자 핵의학 영상기술은 이제 신경과학분야에서도 활발히 적용되고 있으며 신경관련 기초연구나 뇌질환 관련 신약개발에 이미 중요한 역할을 하고 있다. 최근에는 살아있는 개체에서 약제 투여가 뇌에 미치는 약물학적, 생리적 영향을 조사하는 데에도 이용되고 있다. 다가오는 미래에는 각종 뇌질환에서 특이적 표적을 공략하는 새로운 분자치료가 개발되어 뇌질환 치료에 혁명적인 변화를 가져올 것으로 예상되고 있다. 그 예로, 파킨슨씨 병과 같은 퇴행성 신경질환에 줄기세포를 이용한 자가수선, 신경보호, 약물분비 치료, 성장인자와의 병행치료 등이 개발되고, 유전자 치료도 이용될 것으로 보인다. 신경 분자 핵의학 영상은 이와 같은 새로운 뇌질환 치료기술의 개발에 있어서 뇌 안에서 일어나는 분자수준의 변화를 실시간으로 모니터링함으로써 관련연구에 크게 기여할 것으로 기대된다.

• Investigative Magnetic Resonance Imaging
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• 제19권4호
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• pp.212-217
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• 2015

Purpose: For a single time-point hyperpolarized $^{13}C$ magnetic resonance spectroscopy imaging (MRSI) of animal models, scan-time window after injecting substrates is critical in terms of signal-to-noise ratio (SNR) of downstream metabolites. Pre-scans of time-resolved magnetic resonance spectroscopy (MRS) can be performed to determine the scan-time window. In this study, based on two-site exchange model, protocol-specific simulation approaches were developed for $^{13}C$ MRSI and the optimal scan-time window was determined to maximize the SNR of downstream metabolites. Materials and Methods: The arterial input function and conversion rate constant from injected substrates (pyruvate) to downstream metabolite (lactate) were precalibrated, based on pre-scans of time-resolved MRS. MRSI was simulated using two-site exchange model with considerations of scan parameters of MRSI. Optimal scan-time window for mapping lactate was chosen from simulated lactate intensity maps. The performance was validated by multiple in vivo experiments of BALB/C nude mice with MDA-MB-231 breast tumor cells. As a comparison, MRSI were performed with other scan-time windows simply chosen from the lactate signal intensities of pre-scan time-resolved MRS. Results: The optimal scan timing for our animal models was determined by simulation, and was found to be 15 s after injection of the pyruvate. Compared to the simple approach, we observed that the lactate peak signal to noise ratio (PSNR) was increased by 230%. Conclusion: Optimal scan timing to measure downstream metabolites using hyperpolarized $^{13}C$ MRSI can be determined by the proposed protocol-specific simulation approaches.

• 한국농림기상학회지
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• 제4권3호
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• pp.133-140
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• 2002

Site-specific minimum temperature forecasts are critical in a short-term decision making procedure for preventive measures as well as a long-term strategy such as site selection in fruits industry. Nocturnal cold air pools frequently termed in mountainous areas under anticyclonic systems are very dangerous to the flowering buds in spring over Korea, but the spatial resolution to detect them exceeds the current weather forecast scale. To supplement the insufficient spatial resolution of official forecasts, we developed a GIS - assisted frost risk assesment scheme for using in mountainous areas. Daily minimum temperature data were obtained from 6 sites located in a 2.1 by 2.1 km area with complex topography near the southern edge of Sobaek mountains during radiative cooling nights in spring 2001. A digital elevation model with a 10 m spatial resolution was prepared for the entire study area and the cold air inflow was simulated for each grid cell by counting the number of surrounding cells coming into the processing cell. Primitive temperature surfaces were prepared for the corresponding dates by interpolating the Korea Meteorological Administration's automated observational data with the lapse rate correction. The cell temperature values corresponding to the 6 observation sites were extracted from the primitive temperature surface, and subtracted from the observed values to obtain the estimation error. The errors were regressed to the flow accumulation at the corresponding cells, delineating a statistically significant relationship. When we applied this relationship to the primitive temperature surfaces of frost nights during April 2002, there was a good agreement with the observations, showing a feasibility of site-specific frost warning system development in mountainous areas.

• 농촌계획
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• 제13권1호
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• pp.41-50
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• 2007

Recently, it is demanded to study about landscape. Landscape is an environment factor for improving life, a social resource for establishing image and identity of the area and also a tourist resource for earning profit. With this importance, landscape planning is being performed by local governments. However, classification for a rule about landscape planning is not prepared yet. For this purpose, classification of landscape is definitely required. Therefore, this article focuses on presenting method of classification Natural and Rural Landscape. We used Arcview 3.2 to draw watershed of the site, and calculated the percent of landform. We also took a picture which explain the landscape and made a survey of classifying the landscape. Due to this study, we were able to frame an algorithm of the landscape classification. This will contribute to classify the landscape type. This study needs more specific researches because it was supposed to target the entire Gyeonggi-Do however it only covered several regions. Through this results, it would be expected to develop the map of landscape character.

• Journal of Microbiology
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• 제41권3호
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• pp.239-247
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• 2003

The LRV1-4 capsid protein possesses an endoribonuclease activity that is responsible for the single site-specific cleavage in the 5' untranslated region (UTR) of its own viral RNA genome and the formation of a conserved stem-loop structure (stem-loop IV) in the UTR is essential for the accurate RNA cleavage by the capsid protein. To delineate the nucleotide sequences, which are essential for the correct formation of the stem-loop structure for the accurate RNA cleavage by the viral capsid protein, a wildtype minimal RNA transcript (RNA 5' 249-342) and several synthetic RNA transcripts encoding point-mutations in the stem-loop region were generated in an in vitro transcription system, and used as substrates for the RNA cleavage assay and RNase mapping studies. When the RNA 5' 249-342 transcript was subjected to RNase T1 and A mapping studies, the results showed that the predicted RNA secondary structure in the stem-loop region using FOLD analysis only existed in the presence of Mg$\^$2＋/ ions, suggesting that the metal ion stabilizes the stem-loop structure of the substrate RNA in solution. When point-mutated RNA substrates were used in the RNA cleavage assay and RNase T1 mapping study, the specific nucleotide sequences in the stem-loop region were not required for the accurate RNA cleavage by the viral capsid protein, but the formation of a stem-loop like structure in a region (nucleotides from 267 to 287) stabilized by Mg$\^$2＋/ ions was critical for the accurate RNA cleavage. The RNase T1 mapping and EMSA studies revealed that the Ca$\^$2＋/ and Mn$\^$2＋/ ions, among the reagents tested, could change the mobility of the substrate RNA 5' 249-342 on a gel similarly to that of Mg$\^$2＋/ ions, but only Ca$\^$2＋/ ions identically showed the stabilizing effect of Mg$\^$2＋/ ions on the stem-loop structure, suggesting that binding of the metal ions (Mg$\^$2＋/ or Ca$\^$2＋/) onto the RNA substrate in solution causes change and stabilization of the RNA stem-loop structure, and only the substrate RNA with a rigid stem-loop structure in the essential region can be accurately cleaved by the LRV1-4 viral capsid protein.