• Mass Spectrometry Letters
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• 제8권4호
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• pp.69-78
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• 2017

Glycosylation, which is one of the most common post-translation modification (PTMs) of proteins, plays a variety of crucial roles in many cellular events and biotherapeutics. Recent advances have led to the development of various analytical methods employing a mass spectrometry for glycomic and glycoproteomic study. However, site-specific glycosylation analysis is still a relatively new area with high potential for technologies and method development. This review will cover current MS-based workflows and technologies for site-specific mapping of glycosylation ranging from glycopeptide preparation to MS analysis. Bioinformatic tools for comprehensive analysis of glycoprotein with high-throughput manner will be also included.

• 한국초지조사료학회지
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• 제35권3호
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• pp.225-231
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• 2015

Improving current pasture productivity by precision management requires practical tools to collect site specific pasture biomass data. Recent developments in unmanned aerial vehicle (UAV) technology provide cost effective and real time applications for site specific data collection. For the mapping of herbage biomass (BM) on a hill pasture, we tested a UAV system with digital cameras (visible and near-infrared (NIR) camera). The field measurements were conducted on the grazing hill pasture at Hanwoo Improvement Office, Seosan City, Chungcheongnam-do Province, Korea on May 17 and June 27, 2014. Plant samples were obtained from 28 sites. A UAV system was used to obtain aerial photos from a height of approximately 50 m (approximately 30 cm spatial resolution). Normalized digital number (DN) values of Red and NIR channels were extracted from the aerial photos and a normalized differential vegetation index using DN ($NDVI_{dn}$) was calculated. The results show that the correlation coefficient between BM and $NDVI_{dn}$ was 0.88. For the precision management of hilly grazing pastures, UAV monitoring systems can be a quick and cost effective tool to obtain site-specific herbage BM data.

• Mass Spectrometry Letters
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• 제4권1호
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• pp.10-17
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• 2013

Recent developments in MS-based glycomics and glycoproteomics have rapidly advanced the field and pushed the boundaries of glyco-analysis into new territories. This review will lay out current workflows and strategies for characterization of the glycoproteome, including (in order of increasing complexity and information content) preliminary site mapping, compositional glycan profiling, isomer-specific glycan profiling, glycosite-specific glycopeptide profiling, and finally, glycoproteomic profiling.

• Journal of Microbiology
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• 제42권4호
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• pp.361-364
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• 2004

Internal ribosome entry site (IRES) of the hepatitis C virus (HCV) is known to be essential for HCV replication and most conserved among HCV variants. Hence, IRES RNA is a good therapeutic target for RNA-based inhibitors, such as ribozymes. We previously proposed a new anti-HCV modulation strategy based on trans-splicing ribozymes, which can selectively replace HCV transcripts with a new RNA that exerts anti-HCV activity. To explore this procedure, sites which are accessible to ribozymes in HCV IRES were previously determined by employing an RNA mapping method in vitro. In this study, we evaluate the intracellular accessibility of the ribozymes by comparing the trans-splicing activ­ities in cells of several ribozymes targeting different sites of the HCV IRES RNA. We assessed the intra­cellular activities of the ribozymes by monitoring their target-specific induction degree of both reporter gene activity and cytotoxin expression. The ribozyme capable of targeting the most accessible site iden­tified by the mapping studies then harbored the most active trans-splicing activity in cells. These results suggest that the target sites predicted to be accessible are truly the most accessible in the cells, and thus, could be applied to the development of various RNA-based anti-HCV therapies.

• BMB Reports
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• 제42권12호
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• pp.788-793
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• 2009

TLX2 is an orphan homeodomain transcription factor whose expression is mainly associated with tissues derived from neural crest cells. Recently, we have demonstrated that PHOX2A and PHOX2B are able to enhance the neural cell-type specific expression of human TLX2 by binding distally the 5' -flanking region. In the present work, to deepen into the TLX2 transcription regulation, we have focused on the proximal 5'-flanking region of the gene, mapping the transcription start site and identifying a minimal promoter necessary and sufficient for the basal transcription in cell lines from different origin. Site-directed mutagenesis has allowed to demonstrate that the integrity of this sequence is crucial for gene expression, while electrophoretic mobility shift assays and chromatin immunoprecipitation experiments have revealed that such an activity is dependent on the binding of a PBX factor. Consistent with these findings, such a basal promoter activity has resulted to be enhanced by the previously reported PHOX2-responding sequence.

• Molecules and Cells
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• 제38권11호
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• pp.1007-1012
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• 2015

EphA7 is a key molecule in regulating the development of the dien- and mesencephalon. To get insight into the mechanism of how EphA7 gene expression is regulated during the dorsal specification of the dien- and mesencephalon, we investigated the cis-acting regulatory sequence driving EphA7 to the dorsal midline of the dien- and mesencephalon. Transgenic LacZ reporter analysis, using overlapping EphA7 BACs, was used to narrow down the dorsal midline-specific enhancer, revealing the 25.3 kb genomic region as the enhancer candidate. Strikingly, this genomic DNA was located far downstream of the EphA7 transcription start site, +302.6 kb to +327.9 kb. Further enhancer mapping, using comparative genomic analysis and transgenic methods, showed that the 187 bp genomic DNA alone, approximately 305 kb downstream of the EphA7 transcription start site, was sufficient to act as the dorsal midline-specific enhancer of EphA7. Importantly, our results indicate that the 187 bp dorsal midline-specific enhancer is critically regulated by homeobox transcription factors during the development of the dien- and mesencephalon.

• Korean Journal of Radiology
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• 제24권6호
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• pp.553-563
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• 2023

Objective: Functional magnetic resonance imaging (fMRI) and diffusion tensor imaging-derived tractography (DTI-t) contribute to the localization of language areas, but their accuracy remains controversial. This study aimed to investigate the diagnostic performance of preoperative fMRI and DTI-t obtained with a simultaneous multi-slice technique using intraoperative direct cortical stimulation (DCS) or corticocortical evoked potential (CCEP) as reference standards. Materials and Methods: This prospective study included 26 patients (23-74 years; male:female, 13:13) with tumors in the vicinity of Broca's area who underwent preoperative fMRI and DTI-t. A site-by-site comparison between preoperative (fMRI and DTI-t) and intraoperative language mapping (DCS or CCEP) was performed for 226 cortical sites to calculate the sensitivity and specificity of fMRI and DTI-t for mapping Broca's areas. For sites with positive signals on fMRI or DTI-t, the true-positive rate (TPR) was calculated based on the concordance and discordance between fMRI and DTI-t. Results: Among 226 cortical sites, DCS was performed in 100 sites and CCEP was performed in 166 sites. The specificities of fMRI and DTI-t ranged from 72.4% (63/87) to 96.8% (122/126), respectively. The sensitivities of fMRI (except for verb generation) and DTI-t were 69.2% (9/13) to 92.3% (12/13) with DCS as the reference standard, and 40.0% (16/40) or lower with CCEP as the reference standard. For sites with preoperative fMRI or DTI-t positivity (n = 82), the TPR was high when fMRI and DTI-t were concordant (81.2% and 100% using DCS and CCEP, respectively, as the reference standards) and low when fMRI and DTI-t were discordant (≤ 24.2%). Conclusion: fMRI and DTI-t are sensitive and specific for mapping Broca's area compared with DCS and specific but insensitive compared with CCEP. A site with a positive signal on both fMRI and DTI-t represents a high probability of being an essential language area.

• 한국지진공학회논문집
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• 제20권3호
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• pp.171-180
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• 2016

Earthquake event keeps increasing every year, and the recent cases of earthquake hazards invoke the necessity of seismic study in Korea, as geotechnical earthquake hazards, such as strong ground motion, liquefaction and landslides, are a significant threat to structures in industrial hub areas including coastal facilities. In this study, systemized framework of integrated assessment of earthquake-induced geotechnical hazard was established using advanced geospatial database. And a visible simulation of the framework was specifically conducted at two coastal facility areas in Incheon. First, the geospatial-grid information in the 3D domain were constructed with geostatistical interpolation method composed of multiple geospatial coverage mapping and 3D integration of geo-layer construction considering spatial outliers and geotechnical uncertainty. Second, the behavior of site-specific seismic responses were assessed by incorporating the depth to bedrock, mean shear wave velocity of the upper 30 m, and characteristic site period based on the geospatial-grid. Third, the normalized correlations between rock-outcrop accelerations and the maximum accelerations of each grid were determined considering the site-specific seismic response characteristics. Fourth, the potential damage due to liquefaction was estimated by combining the geospatial-grid and accelerations correlation grid based on the simplified liquefaction potential index evaluation method.

• 한국농림기상학회지
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• 제17권2호
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• pp.126-135
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• 2015

본 연구는 전라북도 남원시의 맞춤형 조림지도인 적지적수도를 제작하기 위해 수행하였다. 이를 위해 남원시에 분포하는 주요 수종별 지위지수 추정식을 개발하고, 수종별로 적지에 적합한 입지조건을 반영한 생육범위를 고려하여 각 수종의 적지분포를 공간적으로 평가하였다. 남원시에 분포하는 주요 8개 수종별 지위지수 추정식은 각각 4-5개의 환경인자 조합이 관여하는 것으로 분석되었으며, 전체 27개의 환경인자 중에서 15개가 수종별 지위지수 추정에 사용되었다. 특히 사면위치($X_7$)와 경사형태($X_8$), B층 건습도($X_{11}$), 연최저 기온($X_{15}$), 온량지수($X_{16}$) 비생장기간 5개월 동안의 총강수량($X_{23}$), 월평균 상대습도($X_{24}$), 그리고 생장기간 초기 3개월 동안의 월평균 상대습도($X_{25}$)가 수종별 지위지수 추정식에 많이 포함되는 것으로 판명되었다. 이상과 같은 방법으로 남원시의 적지적수도를 제작한 후, 적합성 검증을 위해 현지 확인 조사를 수행하였다. 현지 확인 결과 본 연구에서 제작한 적지적수도는 사용 가능한 자료의 한계에도 불구하고 수종별 적지분포를 비교적 무난하게 표현하고 있는 것으로 평가되었다. 하지만 부적지로 평가된 일부 지역의 경우 수정 및 보완이 필요한 것으로 나타났다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2002년도 봄 학술발표회 논문집
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• pp.165-172
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• 2002

We presents an hazard zonation mapping technique in karst terrain and its assessment. From the detailed engineering geological mapping. Controlling factors of sink hole and limestone cave formation were discussed and 4 main hazard factors affecting hazard potential are identified as follows: prerequisite hazard factor(distributions of pre-existing sink holes and cavities), geomorphological hazard factors(slope gradient, vegetation, and drainage pattern etc.) geological hazard factors(lithology, fracture patterns and geological structures etc.) and hydraulic conditions(hydraulic head, annual fluctuation of ground water table and composition of g/w water). From the construction of hazard zonation map along the Jecheon-Maepo area, and vertical cross-sectional hazard zonations specific tunnel site we suggest hazard zonation rating systems.