• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.3
• /
• pp.398-407
• /
• 2020

Objective: The Yip1 domain family (YIPF) proteins were proposed to function in endoplasmic reticulum (ER) to Golgi transport and maintenance of the morphology of the Golgi, which were homologues of yeast Yip1p and Yif1p. YIPF3, the member 3 of YIPF family was a homolog of Yif1p. The aim of present study was to investigate the expression and regulation mechanism of porcine YIPF3. Methods: Quantitative realtime polymerase chain reaction (qPCR) was used to analyze porcine YIPF3 mRNA expression pattern in different tissues and pig kidney epithelial (PK15) cells stimulated by polyinosine-polycytidylic acid (poly [I:C]). Site-directed mutations combined with dual luciferase reporter assays and electrophoretic mobility shift assay (EMSA) were employed to reveal transcription regulation mechanism of porcine YIPF3. Results: Results showed that the mRNA of porcine YIPF3 (pYIPF3) was widely expressed with the highest levels in lymph and lung followed by spleen and liver, while weak in heart and skeletal muscle. Subcellular localization results indicated that it expressed in Golgi apparatus and plasma membranes. Upon stimulation with poly (I:C), the level of this gene was dramatically up-regulated in a time- and concentration-dependent manner. pYIPF3 core promoter region harbored three cis-acting elements which were bound by ETS proto-oncogene 2 (ETS2), zinc finger and BTB domain containing 4 (ZBTB4), and zinc finger and BTB domain containing 14 (ZBTB14), respectively. In which, ETS2 and ZBTB4 both promoted pYIPF3 transcription activity while ZBTB14 inhibited it, and these three transcription factors all played important regulation roles in tumorigenesis and apoptosis. Conclusion: The pYIPF3 mRNA expression was regulated by ETS2, ZBTB4, and ZBTB14, and its higher expression in immune organs might contribute to enhancing ER to Golgi transport of proteins, thus adapting to the immune response.

• Radiation Oncology Journal
• /
• v.29 no.3
• /
• pp.191-198
• /
• 2011

Purpose: Although the role of squamous cell carcinoma antigen (SCC-Ag) as a predictive and prognostic factor for uterine cervical cancer has been identified in previous studies, 1) the effective patient group of screening for recurrence with SCC-Ag, 2) the relationship between SCC-Ag and recurrence site, and 3) the relationship between the change of SCC-Ag and treatment outcome or recurrence have not been described. Materials and Methods: The study included 506 patients with histologically proven uterine cervical cancer between January 1994 and December 2010. We determining the serum SCC-Ag level before treatment and after treatment, and conducted a retrospective review of the patients' records. We evaluated the sensitivity and specificity of SCC-Ag for the detection of tumor recurrence by comparing biochemical recurrence with clinical recurrence. Results: The pretreatment SCC-Ag level and the proportion of patients over 1.5 ng/mL were higher in poor prognostic patient group. In the univariate and multivariate analysis, pretreatment SCC-Ag showed a statistically significant correlation with tumor size, International Federation of Gynecology and Obstetrics (FIGO) stage, pathology. In patients with biochemical recurrence vs. those without, 5-year DFS and OS were 27.6 vs. 92.7% (p ${\leq}$ 0.001) and 53.7 vs. 92.5% (p ${\leq}$ 0.001), respectively. Conclusion: Our study reconfirmed the known function of pretreatment SCC-Ag, but could not confirm the function of biochemical response as a predictive factor for treatment and as a prognostic factor. There was no statistically significant relationship between SCC-Ag level and recurrence site. We confirmed the role of SCC-Ag as a follow-up tool for recurrence of disease and which patient groups SCC-Ag was more useful for.

• Proceedings of the Korean Geotechical Society Conference
• /
• 1995.10a
• /
• pp.15.2-22
• /
• 1995

Evaluating stiffness of near-surface materials has been one of the critically important tasks in many civil engineering works. It is the main goal of geotechnical characterization. The so-called deflection-response method evaluates the stiffness by measuring stress-strain behavior of the materials caused by static or dynamic load. This method, however, evaluates the overall stiffness and the stiffness variation with depth cannot be obtained. Furthermore, evaluation of a large-area geotechnical site by this method can be time-consuming, expensive, and damaging to many surface points of the site. Wave-propagation method, on the other hand, measures seismic velocities at different depths and stiffness profile (stiffness change with depth) can be obtained from the measured velocity data. The stiffness profile is often expressed by shear-wave (S-wave) velocity change with depth because S-wave velocity is proportional to the shear modulus. that is a direct indicator of stiffiiess. The crosshole and downhole method measures the seismic velocity by placing sources and receivers (geophones) at different depths in a borehole. Requirement of borehole installation makes this method also time-consuming, expensive, and damaging to the sites. Spectral-Analysis-of-Surface-Waves (SASW) method places both source and receivers at the surface, and records horizontally-propagating surface waves. Based upon the theory of surfacewave dispersion, the seismic velocities at different depths are calculated by analyzing the recorded surface-wave data. This method can be nondestructive to the sites. However, because only two receivers are used, the method requires multiple measurements with different field setups and, therefore, the method often becomes time-consuming and labor-intensive. Furthermore. the inclusion of noise wavefields cannot be handled properly, and this may cause the results by this method inaccurate. When multi-channel recording method is employed during the measurement of surface-waves, there are several benefits. First, usually single measurement is enough because multiple number (twelve or more) of receivers are used. Second, noise inclusion can be detected by coherency checking on the multi-channel data and handled properly so that it does not decrease the accuracy of the result. Third, various kinds of multi-channel processing techniques can be applied to f1lter unwanted noise wavefields and also to analyze the surface-wavefields more accurately and efficiently. In this way, the accuracy of the result by the method can be significantly improved. Fourth, the entire system of source, receivers, and recording-processing device can be tied into one unit, and the unit can be pulled by a small vehicle, making the survey speed very fast. In all these senses, multi-channel recording of surface waves is best suited for a routine method for geotechnical characterization in most of civil engineering works.

• Journal of the Korea Concrete Institute
• /
• v.24 no.6
• /
• pp.633-641
• /
• 2012

In this study, the effects of the inelastic shear behavior of beam-column joint on the response of RC OMRF are evaluated in the inelastic time history analysis. For an example, a 5-story structure for site class SB and seismic design category C was designed in accordance with KBC2009. Bending moment-curvature relationship for beam and column was evaluated using fiber model and bending moment-rotation relationship for beam-column joint was calculated using simple and unified joint shear behavior model and moment equilibrium relationship. The hysteretic behavior was simulated using three-parameter model suggested in IDARC program. The inelastic time history analysis with PGA for return period of 2400 years showed that the model with inelastic beam-column joint yielded smaller maximum base shear force but nearly equivalent maximum roof displacement and maximum story drift as those obtained from analysis using rigid joint. The maximum story drift satisfied the criteria of KBC2009. Therefore, the inelastic shear behavior of beam-column joint could be neglected in the structural design.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.20 no.1
• /
• pp.104-111
• /
• 2016

The steel frame modular housing of which the research and development has been actively carried out recently cannot be constructed through monolithic placement like the reinforced concrete deck of general structure due to the characteristics of construction method of production in the factory and assembly on the site. And floor vertical vibration and deflection caused by inhabitants' activities may become an important issue in the aspect of usability evaluation due to a decrease in the section size of member, a decrease in weight, and so on. Therefore, this study evaluated the vibration performance of deck by using formula of AISC Design Guide 11(hereinafter AISC formula) which was practically used in general for modules where a stud was and wasn't installed at the center of beam in the longitudinal direction in the modular housing to be studied, and examined the applicability of AISC formula through comparison with the results of analysis using a general-purpose analysis program. On the basis of this, a structural cause for an error to occur between analysis result and AISC formula in the deck of module in which a stud was installed was analysed, and measures for considering this were suggested. Besides, an analysis model with the variables of measures for improving the floor vibration performance of modular housing to be studied was established. And measures having excellent vibration performance and economic feasibility were suggested through vibration response analysis and economic evaluation.

• Journal of the Korean School Mathematics Society
• /
• v.4 no.2
• /
• pp.49-60
• /
• 2001

This paper is dedicated to the enhancement of the second-grade students of middle school students' understanding of the basic principles and concepts of probability with the help of Web-browser-used WBI programs relating to probability and statistics in teaching them the unit of probability. I have come up with the following research problems with a view to applying and analyzing the findings. (1) A web sites in relation to the learning of probability and statistics is to be developed with the second graders of middle school in mind. (2) Is there any difference between the experiment group exposed to web-sites-aided classes and the control group going through the conventional ways of math classes when the sites is made trial use of \ulcorner (3) What response do the learners show in regard to classes which makes use of the web sites when the sites is applied\ulcorner After making an experiment with the aid of the web sites and teaching plans, I made a measurement and an analysis of the academic achievement of the subjects, their understanding of classes based on the web sites, the concentration of the learners, and their responses relating to the contents of web sites concentrating on probability and statistics. I have come to the following conclusions based on the findings. First there was a significant difference between the classes of honor students and the classes of backward pupils when T-Test was made with regard to the achievement of the subjects who divided into the experiment group and the control group. The former made use of the web sites whereas the latter stuck to the conventional way of teaching math. The former scored higher than the latter. Second, the research based on questionnaire findings shows that the web-site-aided experiment classes proved to be more effective than the traditional control classes. All in all, I think that web-aided classes will have a more positive influence on the students' interest in math and on the scholastic achievement of math than conventional classes.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.20 no.2
• /
• pp.91-99
• /
• 2017

The parametric study was performed for performance enhancement of wave energy converter(WEC) using a submerged pendulum plate. The wave exciting moment and hydrodynamic moment were obtained by means of eigenfunction expansion method based on the linear potential theory, and then the roll response of a pendulum plate and time averaged extracted power were investigated. The optimal PTO damping coefficient was suggested to give optimal extracted power. The peak value of optimal extracted power occurs at the resonant frequency. The resonant peak and it's width increase, as the height and thickness of a pendulum plate increase. The mooring line installed at the end of the pendulum plate is effective for extracting wave energy because it can not only induce the resonance with the waves of the installation site but also increase the restoring moment in case of PTO-on. The WEC using a rolling pendulum plate suitable for the shallow water acts as breakwater as well as energy extraction device.

• Korean Journal of Occupational Health Nursing
• /
• v.14 no.2
• /
• pp.164-170
• /
• 2005

Purpose: The present study attempted to find subjective musculoskeletal symptoms of operating room nurses (OR nurses) and then to use them as basic data for prevention and management of musculoskeletal symptoms of OR nurses. Method: This study was an exploratory research, and data were collected from OR nurses working in 8 polyclinics in Korea from July 26 to August 19, 2004, using a self-report questionnaire. The questionnaire contains items concerned with subjective musculoskeletal symptoms include ones with the presence of symptoms and with the degree of discomfort, in the joints such as neck, shoulder, arm/elbow, hand/wrist/finger and waist. For data collection, the aim of the study was explained to the operating room managers to obtain their help, and these questionnaires were sent to hospitals, and were retrieved by post. 271(90.3%) questionnaires were returned among those sent to 300 nurses. 249 questionnaires excluding 22 insufficient ones such as no response were used for data analysis. Data were analyzed using SPSS WIN 12.0. Subjective musculoskeletal symptoms were analyzed in the number and percentage. Result: 187(75.1%) nurses said they 'had' subjective musculoskeletal symptoms and 62 (24.9%) said they had 'nothing'. 130(52.2%), 125 (50.2%), 113 (45.4%), 86(34.5%), and 42 (16.9%) nurses had subjective musculoskeletal symptoms in waist, shoulder, hand/wrist/finger, neck, and arm/elbow, respectively. 51(27.3%) and 136 (72.7%) nurses complained of the pain in one site, and in two or more sites, respectively. In particular, 51% and 47.4% nurses said that they were 'discomforted' due to the pain in waist and in shoulder, respectively. Conclusion: Subjective musculoskeletal symptoms which OR nurses complained of were significant. This may cause difficulty in nursing tasks in the operating room. So various arrangements have to be made for OR nurse with subjective musculoskeletal symptoms at an early stage.

• Journal of Soil and Groundwater Environment
• /
• v.25 no.1
• /
• pp.25-36
• /
• 2020

In a redox transition zone, geochemical reactions are facilitated by active bacteria that mediate reactions involving electrons, and arsenic (As) and iron (Fe) cycles are the major electron transfer reactions occurring at such a site. In this study, the effect of repetitive redox changes on soil bacterial community in As-contaminated soil was investigated. The results revealed that bacterial community changed actively in response to redox changes, and bacterial diversity gradually decreased as the cycle repeated. Proportion of strict aerobes and anaerobes decreased, while microaerophilic species such as Azospirillum oryzae group became the predominant species, accounting for 72.7% of the total counts after four weeks of incubation. Bacterial species capable of reducing Fe or As (e.g., Clostridium, Desulfitobacterium) belonging to diverse phylogenetic groups were detected. Indices representing richness (i.e., Chao 1) and phylogenetic diversity decreased from 1,868 and 1,926 to 848 and 1,121, respectively. Principle component analysis suggests that repetitive redox fluctuation, rather than oxic or anoxic status itself, is an important factor in determining the change of soil bacterial community, which in turn affects the cycling of As and Fe in redox transition zones.

• Korean Journal of Head & Neck Oncology
• /
• v.12 no.1
• /
• pp.32-42
• /
• 1996

This study was desinged to evaluate the effect of radioactive iodine-131 therapy in differentiated thyroid carcinoma treated at the Presbyterian Medical Center, Chonju during the 20-year period from 1975 to 1994. The authors reviewed 246 patients who received radioactive iodine-131. An analysis of the therapeutic response and survival rates of the 246 patients has been carried out. The male to female ratio was 1 : 3.6. The peak incidence was in the 4th and 6th decades. The histologic findings in the 246 patients were papillary adenocarcinoma in 200 cases, follicular adenocarcinoma in 29 cases, mixed type in 14 cases, and others in 3 cases. Combined treatment modalities of 246 patients consisted of sugery and radioiodine in 222 cases, surgery with radioiodine and external irradiation in 11 cases, and surgery with radioiodine, external irradiation and chemotherapy in 5 cases. 42 of the 246 cases showed recurrence and the commonest type of combined treatment for recurrent case was surgery followed by radioiodine-131. The highest accumulated total dosage of radioiodine-131 was 480mCi in that case femoral metastasis was noticed. The most common locoregional metastatic site was ipsilateral cervical node, and neighbouring muscle, vessel, trachea, recurrent layngeal nerve, in order of frequency. The determinate 10-year survival rate was 91.8% in the group receiving surgery followed by radioiodine-13l and 71.4% in patients receiving surgery, radioiodine-13l with XRT. The determinate 10-year survival rate was better for patients under 40 years of age who received radioiodine as compared to patients over 40 year of age(85.7% vs. 33.3%). The most usual primary therapeutic dosage in the group of cervical lesion was 90$\sim$120mCi after surgery.