• Journal of Life Science
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• v.9 no.1
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• pp.50-57
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• 1999

STATs activated by various cytokine and growth factors trigger a quick response in the nucleus and induce changes in gene expression. We have found the sequences homologous to STAT binding site in the 5'-flanking region of the D-raf gene. In this study, we examined role of the STAT binding site in D-raf gene promoter activity in vivo by using transgenic flies. The reporter plasmid pDraf-STATmut-lacZ was constructed by fusing D-raf promoter fragment having the base-substituted STAT binding site with the lacZ gene in a P-element vector. Transgenic flies bearing the Draf-STATmut-lacZ fusion genes were established by P-element mediated transformation. The expression of lacZ in transgenic flies bearing Draf-STATmut-lacZ fusion genes carrying base substitution in STAT site throughout various developmental stages was extensively reduced in comparison with that in transgenic flies bearing wild type Draf-lacZ fusion gene. These results show that the STAT binding site plays an important role in regulation of the D-raf gene.

• Journal of Ecology and Environment
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• v.47 no.4
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• pp.228-240
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• 2023

Growing complexity in ecosystem structure and functions, under impacts of climate and land-use changes, requires interdisciplinary understandings of processes and the whole-system, and accurate estimates of the changing functions. In the last three decades, observation networks for biodiversity, ecosystems, and ecosystem functions under climate change, have been developed by interested scientists, research institutions and universities. In this paper we will review (1) the development and on-going activities of those observation networks, (2) some outcomes from forest carbon cycle studies at our super-site "Takayama site" in Japan, and (3) a few ideas how we connect in-situ and satellite observations as well as fill observation gaps in the Asia-Oceania region. There have been many intensive research and networking efforts to promote investigations for ecosystem change and functions (e.g., Long-Term Ecological Research Network), measurements of greenhouse gas, heat, and water fluxes (flux network), and biodiversity from genetic to ecosystem level (Biodiversity Observation Network). Combining those in-situ field research data with modeling analysis and satellite remote sensing allows the research communities to up-scale spatially from local to global, and temporally from the past to future. These observation networks oftern use different methodologies and target different scientific disciplines. However growing needs for comprehensive observations to understand the response of biodiversity and ecosystem functions to climate and societal changes at local, national, regional, and global scales are providing opportunities and expectations to network these networks. Among the challenges to produce and share integrated knowledge on climate, ecosystem functions and biodiversity, filling scale-gaps in space and time among the phenomena is crucial. To showcase such efforts, interdisciplinary research at 'Takayama super-site' was reviewed by focusing on studies on forest carbon cycle and phenology. A key approach to respond to multidisciplinary questions is to integrate in-situ field research, ecosystem modeling, and satellite remote sensing by developing cross-scale methodologies at long-term observation field sites called "super-sites". The research approach at 'Takayama site' in Japan showcases this response to the needs of multidisciplinary questions and further development of terrestrial ecosystem research to address environmental change issues from local to national, regional and global scales.

• Journal of the Earthquake Engineering Society of Korea
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• v.10 no.5 s.51
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• pp.63-71
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• 2006

In seismic response analysis of building structures, the input ground accelerations have considerable effect on the nonlinear response characteristics of structures. The characteristics of soil and the locality of the site where those ground motions were recorded affect on the contents of earthquake waves. Therefore, it is difficult to select appropriate input ground motions for seismic response analysis. This study describes a generation of artificial earthquake wave compatible with seismic design spectrum, and also evaluates the nonlinear response spectra by the simulated earthquake motions. The artificial earthquake wave are generated according to the previously recorded earthquake waves in past earthquake events. The artificial wave have identical phase angles to the recorded earthquake wave, and their overall response spectra are compatible with seismic design spectrum with 5% critical viscous damping. Each simulated earthquake wave has a identical phase angles to the original recorded ground acceleration, and match to design spectra in the range of period from 0.02 to 10.0 seconds. The seismic response analysis is performed to examine the nonlinear response characteristics of SDOF system subjected to the simulated earthquake waves. It was concluded that the artificial earthquake waves simulated in this paper are applicable as input ground motions for a seismic response analysis of building structures.

• Journal of the Earthquake Engineering Society of Korea
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• v.16 no.4
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• pp.1-8
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• 2012

Domestic steel moment resisting frames were designed in accordance with the former KBC2005 and the current KBC2009, and then their seismic performance was evaluated in accordance with FEMA355F by utilizing nonlinear dynamic analysis. The results from the procedure in FEMA355F were different with those from the capacity spectrum method utilizing nonlinear static push-over analysis. In particular, the domestic steel moment resisting frames have a weak panel zone, so their behavior can be estimated more precisely by nonlinear dynamic analysis. The domestic steel moment resisting frames satisfied the performance goal if located at a site class $S_B$ or $S_C$, regardless of the story number and the response modification factor. However, if they are located at a site class $S_D$ or $S_E$, performance goal satisfaction cannot be guaranteed. No matter what standard is used for the design, KBC2005 or KBC2009, the domestic steel moment resisting frames may possess satisfactory seismic performance if the site condition is relatively good.

• Journal of Korean Society of Steel Construction
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• v.19 no.2
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• pp.223-231
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• 2007

Considering wind speed uncertainty, reliability analysis of the LNG unloading arm at Tongyoung Production Site was performed. Extreme distribution of wind speed was estimated from the data collected at the weather center and wind load was calculated using wind velocities and coefficients of wind pressure. The unloading arm was modeled with plate and solid elements. Contact elements were used to describe the interface between base of structure andground. Response surface for maximum effective stress was found for reliability analysis and then reliability functions was defined and used to determine exceeding probability of allowable and yield stresses. In addition, sensitivity analysis was also performed to estimate the effect of possible material deterioration in the future.

• Earthquakes and Structures
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• v.13 no.6
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• pp.561-572
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• 2017

Seismic base isolation has been accepted as one of the most popular design procedures to protect important structures against earthquakes. However, due to lack of information and experimental data the application of base isolation is quite limited to nuclear power plant (NPP) industry. Moreover, the effects of inelastic behavior of soil beneath base-isolated NPP have raised questions to the effectiveness of isolation device. This study applies the wavelet analysis to investigate the effects of soil-structure interaction (SSI) on the seismic response of a base-isolated NPP structure. To evaluate the SSI effects, the NPP structure is modelled as a lumped mass stick model and combined with a soil model using the concept of cone models. The lead rubber bearing (LRB) base isolator is used to adopt the base isolation system. The shear wave velocity of soil is varied to reflect the real rock site conditions of structure. The comparison between seismic performance of isolated structure and non-isolated structure has drawn. The results show that the wavelet analysis proves to be an efficient tool to evaluate the SSI effects on the seismic response of base-isolated structure and the seismic performance of base-isolated NPP is not sensitive to the effects in this case.

• Journal of the Korea Safety Management & Science
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• v.14 no.3
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• pp.39-48
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• 2012

Risk Assessment to list possible safety disasters and their probability and severity is the starting point for effective safety management on construction project site. However, the safety managers in owners, construction supervisors, contractors, and sub-contractors still have difficulties in judging the priorities of safety activities and preparing responses to each potential safety disasters. Therefore, this study aimed to suggest a systematic method in assessing safety risk prior to commencement with the agreement of stakeholders. FMECA(failure mode effects and criticality analysis) was selected as a main assessment tool and it was modified according to the characteristics of construction projects and trades. Each risk is, firstly, evaluated with occurrence probability, possible loss and impacts to projects, and detections, and then risk priority number(RPN) is calculated. Subsequently, the managers of each stakeholder discuss the types, timing, and responsibilities of responses as a group decision-making process.

• Journal of the Ergonomics Society of Korea
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• v.19 no.3
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• pp.61-75
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• 2000

To enhance web site's usability, it has been suggested that the depth of tree structured menus should be minimized. In this research, experimental results are reported to quantitatively compare the methods currently used for reducing the depth of menus in web sites. 25 popular web sites were selected and their menu types were categorized into four types: top menu, drop-down menu, boolean menu, and table of contents. The four types of menu were then sub-categorized into 15 different types according to their sub-menu type, existence of menu colors, and the event occurring after mouse activation. Performance tests and subjective evaluation were carried out. The results showed that there were no significant differences in terms of response time among the 15 menu types, while table of contents and drop-down in which the first and second level of menus were visible induced the least number of errors. In the subjective test, the top-menu structure with colors and presentation of its sub-menu without clicking mouse were preferred.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1999.04a
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• pp.178-185
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• 1999

Minimum life-cycle cost helps to evaluate cost effectiveness of base-isolated bridges under specific condition. Life-cycle cost mainly consists of the initial construction cost and the expected damage cost. Damage cost estimation needs proper model of input ground motion failure probability evaluation method and limit states definition. We model the input ground motion as spectral density function compatible with the response spectra defined at each seismicity and site condition. Spectrum analysis and crossing theory is suitable for reseating calculation of failure probabilities in the process of cost minimization. Limit states of base-isolated bridges re defined for superstructure isolator and pier respectively The method is applied to both base-isolated bridges and conventional bridges under the same conditions to investigate cost effectiveness of base isolation in low and moderate seismic region. the results show that base-isolation of bridges are more effective in low and moderate seismic region and that the site effects on the economical efficiency may not be negligible in such a region.

• Journal of the Society of Disaster Information
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• v.10 no.1
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• pp.33-39
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• 2014

Chemical accidents are the cause of the accident site during the initial responders to quickly and easily see materials and concentration method for the U.S. Environmental Protection Agency(EPA) is widely used in the initial response team direct reading detection equipment used. Ministry of the tubular gas detection equipment to detect direct reading detection equipment used in the event of an accident scene, and shell-and-tube gas detector for rapid detection and identification and precise analysis of causative pollutants before about strategically can identify the quantitative and qualitative useful equipment. However, those who initially respond to the scene of a direct reading detection equipment and a simple lack of understanding of how to use the numbers only because of the way you want to check the accuracy of detection results have been raising questions about the increase. The scene of the accident in order to obtain an accurate detection results used in this paper, the Ministry of Environment of gas detectors detect tubular Kitagawa and Draeger detector tube to check the reactivity of the material on-site detection of early response of those who were to raise the accuracy of the results.