• Journal of the Korean Society of Safety
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• v.33 no.6
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• pp.50-57
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• 2018

The purpose of this study is to evaluate the practical application of the self consolidating concrete for the steel concrete pannel (SCP) in module LNG storage tank proposed in the previous research. We evaluated the physical properties and filling performance of developed concrete for the SCP module. First, a slump flow test was conducted to evaluate the performance of the proposed guidelines for the filling test. As a result, all of the concrete used showed satisfactory performance. Based on the results of the previous study, it was found that the reliability of the required time measured by the $T_{500}$ test and the rheometer results measured before and after pumping was 0.94 which means the separation and blocking should not occur. The L-box test and the U-box test were conducted before and after pumping. All of the guidelines suggested showed satisfactory performance. SCP module for LNG storage tanks was fabricated on actual size scale to evaluate the practical application at the final site. As a result, it was confirmed that satisfactory filling performance was obtained in all the specimens.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1919-1926
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• 2020

CRISPR interference (CRISPRi) has been developed as a transcriptional control tool by inactivating the DNA cleavage ability of Cas9 nucleases to produce dCas9 (deactivated Cas9), and leaving dCas9 the ability to specifically bind to the target DNA sequence. CRISPR/Cas9 technology has limitations in designing target-specific single-guide RNA (sgRNA) due to the dependence of protospacer adjacent motif (PAM) (5'-NGG) for binding target DNAs. Reportedly, Cas9-NG recognizing 5'-NG as the PAM sequence has been constructed by removing the dependence on the last base G of PAM through protein engineering of Cas9. In this study, a dCas9-NG protein was engineered by introducing two active site mutations in Cas9-NG, and its ability to regulate transcription was evaluated in the gal promoter in E. coli. Analysis of cell growth rate, D-galactose consumption rate, and gal transcripts confirmed that dCas9-NG can completely repress the promoter by recognizing DNA targets with PAM of 5'-NGG, NGA, NGC, NGT, and NAG. Our study showed possible PAM sequences for dCas9-NG and provided information on target-specific sgRNA design for regulation of both gene expression and cellular metabolism.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.32 no.5
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• pp.837-844
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• 2022

In proportion to the rapid development of information and communication technology, the damage to copyright infringement is also increasing. In particular, as the OTT platform market has grown significantly in recent years, the speed and distribution of pirated copies that infringe copyright are increasing rapidly compared to the past. Accordingly, the country is trying to prevent copyright infringement by detecting and blocking illegal streaming sites, but it is difficult to expect great results due to the fast production of illegal streaming sites. Therefore, in this paper, we analyze the causes of rapid production of blocked illegal streaming sites, track and analyze 58 illegal streaming sites, and propose ways to effectively block illegal streaming sites based on the analysis results.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.74-74
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• 1995

GABA transaminase is inactivated by preincubation with p$^1$, p$^2$-bis(5'-pyridoxal) diphosphate at pH 7.0. The inactivation under pseudo-first order conditions proceeds at a slow rate (K$\_$obs/=0.035 min$\^$-1/). The degree of labeling of the enzyme by p$^1$, p$^2$-bis(5'-pyridoxal) diphosphate was determined by absorption spectroscopy, The blocking of 2 lysyl residues/dimer is needed for inactivation of the transaminase. The time course of the reaction is significantly affected by the substrate ${\alpha}$-ketoglutarate, which afforded complete protection against the loss of the catalytic activity. Whereas cofator pyridoxal phosphate failed to prevent the inactivation of the enzyme. Therefore, it is postulated that binding of ${\alpha}$-ketoglutarate tn lysyl residues is the major factor contributing to stabilization of the catalytic site and bifuctional reagent p$^1$, p$^2$bis(5'-pyridoxal) diphosphate blocks lysyl residues other than those involved in the binding of the cofactor.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.5
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• pp.100-108
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• 2012

The purpose of the study was to evaluate the influence of shading and ventilation on Mean Radiant Temperature(MRT) of the outdoor space at a summer outdoor. The Wind Speed(WS), Air Temperature(AT) and Globe Temperature(GT) were recorded every minute from $1^{st}$ of May to the $30^{th}$ of September 2011 at a height of 1.2m above in four experimental plots with different shading and ventilating conditions, with a measuring system consisting of a vane type anemometer(Barini Design's BDTH), Resistance Temperature Detector(RTD, Pt-100), standard black globe(${\O}$ 150mm) and data acquisition systems(National Instrument's Labview and Compfile Techs' Moacon). To implement four different ventilating and shading conditions, three hexahedral steel frames, and one natural plot were established in the open grass field. Two of the steel frames had a dimension of $3m(W){\times}3m(L){\times}1.5m(H)$ and every vertical side covered with transparent polyethylene film to prevent lateral ventilation(Ventilation Blocking Plot: VP), and an additional shading curtain was applied on the top side of a frame(Shading and Ventilation Blocking Plot: SVP). The third was $1.5m(W){\times}1.5m(L){\times}1.5m(H)$, only the top side of which was covered by the shading curtain without the lateral film(Shading Plot: SP). The last plot was natural condition without any kind of shading and wind blocking material(Natural Open Plot: NP). Based on the 13,262 records of 44 sunny days, the time serial difference of AT and GT for 24 hour were analyzed and compared, and statistical analysis was done based on the 7,172 records of daytime period from 7 A.M. to 8 P.M., while the relation between the MRT and solar radiation and wind speed was analyzed based on the records of the hottest period from 11 A.M. to 4 P.M.. The major findings were as follows: 1. The peak AT was $40.8^{\circ}C$ at VP and $35.6^{\circ}C$ at SP showing the difference about $5^{\circ}C$, but the difference of average AT was very small within${\pm}1^{\circ}C$. 2. The difference of the peak GT was $12^{\circ}C$ showing $52.5^{\circ}C$ at VP and $40.6^{\circ}C$ at SP, while the gap of average GT between the two plots was $6^{\circ}C$. Comparing all four plots including NP and SVP, it can be said that the shading decrease $6^{\circ}C$ GT while the wind blocking increase $3^{\circ}C$ GT. 3. According to the calculated MRT, the shading has a cooling effect in reducing a maximum of $13^{\circ}C$ and average $9^{\circ}C$ MRT, while the wind blocking has heating effect of increasing average $3^{\circ}C$ MRT. In other words, the MRT of the shaded area with natural ventilation could be cooler than the wind blocking the sunny site to about $16^{\circ}C$ MRT maximum. 4. The regression and correlation tests showed that the shading is more important than the ventilation in reducing the MRT, while both of them do an important role in improving the outdoor thermal comfort. In summary, the results of this study showed that the shade is the first and the ventilation is the second important factor in terms of improving outdoor thermal comfort in summer daylight hours. Therefore, it can be apparently said that the more shade by the forest, shading trees etc., the more effective in conditioning the microclimate of an outdoor space reducing the useless or even harmful heat energy for human activities. Furthermore, the delicately designed wind corridor or outdoor ventilation system can improve even the thermal environment of urban area.

• Journal of Navigation and Port Research
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• v.36 no.8
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• pp.625-630
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• 2012

The DGNSS Central Office operates 17 DGNSS reference stations. Compared to the other DGNSS sites, the TEQC data quality of some sites is poorer. In this study, we tried to find out the causes that degrade the quality of GPS data for the purpose of improving the signal quality of the DGNSS stations. We selected the Chungju station that is the one of those stations with bad data quality. Through the on-site visit, we found that there is no signal-blocking obstacles. In addition to site surveys, we conducted two experiments; simultaneous observation considering environmental factors and comparison test through equipment replacements to check the malfunctioning of GPS equipments. In the simultaneous test results, we realized that environmental factors do not induce any bad effects on the data quality. In equipment replacement experiments, we confirmed that the data quality is of excellent quality when the test receiver was used instead of the original one installed at the site. When we replaced the antenna instead of the receiver, the data quality was bad. Through those two experiments, we concluded that the receiver is the main factor that degrades the signal quality.

• Korean Journal of Heritage: History & Science
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• v.56 no.3
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• pp.46-59
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• 2023

Various Baekje ground-level building sites have been identified, in Pungnaptoseong Fortress, including Mirae Village's site E-1. However, building site E-1 is the only one with excavated roof tiles that are directly connected to the building site. As for building sites E-2, D-1, and D-2, which are comparable to site E-1, it is very possible that they had tiles on the roof based on their jeoksim (blocking facilities for roof slopes) and building structures. Also, although they are semi-underground pit structures, pit building sites A-30 and modern apartment site A-5, as well as the No.44 remains of Gyeongdang District, which is closer to a ground-level type, the buildings with tiles may have been constructed in the form of partial tile roofs rather than full-face tile roofs. Therefore, there may be several reasons behind the use of tiles on roofs in the early days, but the primary background of the building's authoritative function would have been considered first. Considering that China and Japan started using tiles on nationally important buildings such as palaces, temples, and ritual buildings, it may be presumed that Baekje began using tiles from the time it centralized power. It is believed that Baekje's early roof tile buildings evolved from rudimentary residential architecture to advanced public architecture, taking into consideration fire prevention and structural stability in large buildings. It is difficult to find similar cases in Korea with structural features such as the elevated foundations or underground stone foundations that can be found in Mirae Village building site E-1. Rather, similar architectural techniques can be found in China and Japan. In China, similar construction techniques were discovered in buildings of worship that were primarily built in the palace surroundings, such as Jangan Castle. Based on this, it appears that roof tile building sites, such as site E-1, that have been discovered have a strong correlation with the characteristics of buildings of worship, and ground type buildings, such as sites D-1 and D-2, are important facilities that are related to important public facilities such as state-run warehouses. This provides many implications regarding the early Baekje city structure.

• Journal of the Korean Institute of Landscape Architecture
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• v.37 no.5
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• pp.31-41
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• 2009

Under the Korea Building Act, anyone planning to build a building or buildings on a site over $200m^2$ must plant plants over a specific area. In large scale development this rule is adhered to well, but such is not the case in small scale development. Therefore, special attention must be given to small scale development. Thus, the purpose of this research is to analyze the current situation and practice of planting at small scale development sites in Seoul, and then suggest policies for improving them. In this study using the data covering Seongbuk-Gu and Gangnam-Gu, which was surveyed in 2002, the current situation and practice of planting at small scale development sites was analyzed. After a questionnaire survey was conducted with government officers and building owners, the same analysis was made. Then the policies for improvement were extracted. The results are as follows: 1. In superordinate planning stage, because the minimum standards are too low, those must be strengthened. Any district plan does not control planting in private building lots. This requests active application of planting in private building lots as a design control measure in district planning. 2. In the building design stage, there are no guidelines. The obligation of building set-back between adjacent buildings by the Korean Building Act produces mass shaded and inferior planting beds. The act also is blocking landscape architects' participation in small scale development. And wall installations deteriorate the streetscape and growth of plants with shading. Therefore guidelines must be made. 3. In each stage of the building permit, the permit for building completion, and maintenance the Korean Building Act is blocking landscape architects' participation in small scale development, so the planting plan is completely handled by nonprofessional persons. Therefore, the act should be amended in order to make way for landscape architects' participation in each stage of the small scale development process.

• Journal of the Korean Geotechnical Society
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• v.17 no.6
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• pp.47-51
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• 2001

There are two strategies to cope with the troubles in landfill site after closure. The first method is active in a way that the wastes are dug up and the recyclable materials are reutilized, meanwhile the materials not recyclable are incinerated in order to minimize the volume of residues to be disposed of. The second method is rather passive and defensive in a way that the source of contamination, that is, buried wastes are not treated. Instead, the transport of leaking leachate and gases generated from the wastes are intercepted and controlled. In this study, as a passive way of the efficient leachate blocking process, applicabilities of geocrete and soluble sodium silicate as a substitute to control leachate leaking from landfill sidewall were investigated. In case of compression test, the strength of mixture I (Geocrete:Sodium silicate=1:3.9 v/v) and mixture II (Geocrete:Sodium silicate=1:2.5 v/v), even after 7 days' curing was higher than the minimum allowance to tolerate the loading(5 kg/$\textrm{cm}^2$). Soaking in the acid fur 4 days and 7 days respectively, the compressive strength of the specimens reduced seriously. The toxicity of geocrete is not detected through the bioassay test, once it was mixed with sodium silicate and the complex was formed. The hydraulic conductivity of the mixtures even after 7 days' curing was lower than the threshold limit $(1.0\times10_{-7}cm/s)$.

• The Korean Journal of Pharmacology
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• v.2 no.1 s.2
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• pp.71-82
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• 1966

The inhibitory effect of 5-hydroxytryptamine (5-HT) on the isolated intestinal strips of the tortoise (Amyda japonica), rat, rabbit and guinea pig was investigated. 1) The strips from the middle or lower part of the tortoise intestine responded with relaxation to 5-HT $(10^{-9}{\sim}10^{-5}g/ml)$, and the magnitude of the relaxation was proportional to the dose of 5-HT. The rectal part of the tortoise intestine, in contrast, showed contraction, the magnitude of which also was proportional to the dose of 5-HT. 2) Various blocking agents such as methysergide, morphine, tetracaine, nethalide, bretylium, hexamethonium, mecamylamine and chlorisondamine, showed no selective blocking activity on the relaxant effect of 5-HT on the tortoise intestine. The inhibitory effect of isoproterenol on the tortoise intestine, however, was selectively blocked by nethalide, and the stimulatory effect of 5-HT on the rectal part of the tortoise was blocked by methysergide. 3) In the presence of 5-HT, the stimulatory effect of DMPP on the tortoise intestine was remarkably attenuated, whereas that of acetylcholine and $BaCl_2$ was little affected. In the presence of isoproterenol, the stimulatory effect of acetylcholine and $BaCl_2$ were affected, but that of DMPP was little affected. 4) Large dose of 5-HT($10^{-4}$g/ml) produced inhibitory effect on the strips from the distal part of the isolated colon of the rat, rabbit and guinea pig, when the strips had been exposed to 5-HT($10^{-4}$g/ml), methysergide or phen`oxybenzamine. 5) Bretylium, as well as nethalide, abolished or remarkably reduced, in a few cases of the experiments, the inhibitory effect of the large dose of 5-HT on the distal part of the colon, whereas morphine did not affect it. 6) The ileal strips of the guinea pig also showed relaxation, as in the colonic strips, having been exposed to the large dose of 5-HT or phenoxybenzamine. This effect, however, was not obsered in the case of the rabbit ileum. 7) The property of the action-site of 5-HT in the tortoise intestine seemd to be different from the 5-HT receptors which have been revealed by several investigators. 8) Adrenergic component seemed to be participated in the inhibitory effect of 5-HT on the colon of the rat and rabbit.