• 대한한의학방제학회지
• /
• 제16권1호
• /
• pp.109-115
• /
• 2008

Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by micronucleus test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo micronucleus test was performed using 7 weeks ICR mice. At 24 hours after with Sipjeondaebo-tang extract at the doses of 0, 500, 1000 and 2000 mg/kg/day by peritoneal route mice were sacrificed and bone marrow cells were prepared for smear slides. Results : As a result of counting the micronucleus polychromate erythrocyte of 2000 polychromate erythrocyte, all treatment groups did not show statistically significant increase than negative control group. and there was no clinical sign and body weight connected with injection of Sipjeondaebo-tang(Shiquan dabu-decoction) extract. Conclusions: It was concluded that Sipjeondaebo-tang extract did not induce micronucleus in the bone marrow cells of ICR mice

• 대한한의학회지
• /
• 제29권3호
• /
• pp.1-10
• /
• 2008

Objectives: This study was to assess the toxicity of Sipjeondaebo-tang by bacterial reverse mutation test. Methods: In this study, to evaluate the bacterial reverse mutation of Sipjeondaebo-tang water-extract, the in vitro Ames test using Salmonella typhimurium (TA98, TA100, TA1,535, TA1,537) and Escherichia coli(WP2uvrA) were performed with Sipjeondaebo-tang water extract at the concentrations 0, 312, 625, 1250, 2500 and 5000 ${\mu}g/plate$. Results: Sipjeondaebo-tang water extract was negative in Ames test with both Salmonella typhimurium or Escherichia coli with and without rat liver microsomal enzyme (S9- fraction and S+ fraction). Conclusions: According to these results, we concluded that a Sipjeondaebo-tang water extract did not cause bacterial reverse mutation.

• 대한한방내과학회지
• /
• 제37권5호
• /
• pp.770-775
• /
• 2016

Objective: To evaluate the effects of Sipjeondaebo-tang (十全大補湯) extract on a stage two pressure sore. Methods: Sipjeondaebo-tang extract (5 g) was given three times a day to a treat the pressure sore. Results: The pressure sore in the sacrococcygeal region was improved. A second pressure sore ofthe right greater trochanter region, which emerged during the study, was also improved. Conclusions: Sipjeondaebo-tang extract may represent an effective treatment for pressure sores, but further studies are needed.

• 대한한의학방제학회지
• /
• 제16권2호
• /
• pp.145-153
• /
• 2008

Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by Chromosomalanomaly test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo Chromosomalanomaly test was performed using chiness hamster lung cell line. Results : Sipjeondaebo-tang water extract was negative in Chromosomalanomaly test at the doses of 0, 625, 1250 and $2500{\mu}g/m\ell$ at 6h and 24h.(S9- fraction). Chromosomalanomaly test(S+fraction) was also negative at the doses of 0, 1250, 2500 and $5000{\mu}g/m\ell$. Conclusions : It was concluded that Sipjeondaebo-tang extract did not induce Chromosomalanomaly in the chiness hamster lung cell.

• 대한한방내과학회지
• /
• 제32권3호
• /
• pp.334-344
• /
• 2011

Objectives : Sipjeondaebo-tang is a medicine traditionally prescribed as a restorative. The aim of this study was to investigate the single oral dose toxicity and safety of extract of fermented Sipjeondaebo-tang in ICR mice. Methods : In single oral dose toxicity study, non-fermented or fermented Sipjeondaebo-tang were administered by oral gavage to ICR mice (5 males, 5 females) at single doses of varying concentrations: 1250, 2500 and 5000 mg/kg. Changes of body weight, general behavior, adverse effects and mortality were determined throughout the experimental period. Hematological parameters, organ weights and necropsy findings were evaluated at the end of the experiment. Results : There were no mortality or signs of toxicity in single oral dose toxicity studies. There were also no significant differences in body weight, organ weight, or hematological parameters between the treatment and control groups. Conclusions : Fermented Sipjeondaebo-tang did not cause remarkable adverse effects in ICR mice. The oral lethal dose of fermented Sipjeondaebo-tang is more than 5000 mg/kg and no-observed-adverse-effect level (NOAEL) of the extract for both male and female mice is 5000 mg/kg.

• 대한한방부인과학회지
• /
• 제25권2호
• /
• pp.108-119
• /
• 2012

Objectives: This study was performed to compare the differences between decoctions extracted by different extraction method and extraction time. Methods: Decoctions were prepared with pressurized or non-pressurized extraction for 60, 120 and 180min. The yield of extract, total soluble solid content, hydrogen ion concentration(pH) and the content of reference compound in Sipjeondaebo-tang (Siquandabu-tang) were investigated. Results: While yields and the total soluble solid of decoction were higher in pressurized method proportional to extraction time, pH values were lower in pressurized method and showed decreasing values with increasing extraction time. Albilflorin, ferulic acid, nodakenin, coumarin, cinnamaldehyde and glycyrrhizin were contained in decoctions by pressurized extraction method more than non-pressurized method whereas the content of coumarin was higher in decoctions by non-pressurized extraction method. In addition, coumarin was extracted increasingly with extraction time in decoctions by pressurized method, however, nine compounds except cinnamaldehyde showed the tendency of increasing proportional to extraction time. Conclusions: The results show that extraction methods including pressurized or non-pressurized extraction, and extraction time could affect the physicochemical characteristic or composition of Sipjeondaebo-tang(Siquandabu-tang) decoction.

• 대한한방내과학회지
• /
• 제31권2호
• /
• pp.212-223
• /
• 2010

Objectives : The aims of this study were to evaluate whether gastric contractility could be measured by bowel sound of rats, and whether gastrointestinal side effect of any herbal restoratives would be related with their inhibition of gastric contraction. Methods : Streptozotocin (STZ)-induced diabetic rats were used as a gastric hypocontraction model. At the time of 6 weeks after induction of diabetes, 2mL of normal saline (NS) and 2mL of extract solution each containing 125mg/kg of Bojoongikki-tang (BJ), Sipjeondaebo-tang (SJ), Youngkaechulgam-tang (YG) were given to Normal (NR) and diabetic rats (DR), respectively. Bowel sound was recorded for 30 minute in fasting, and 120 minute of their administration. Gastric motility index was serially calculated with the ratio of accumulated potentials of post-administration/fasting state every 10 minutes. Results : Gastric contractility between NR and DR could be significantly distinguished by bowel sound auscultation. BJ had a decreasing effect, SJ had a bidirectional effect, and YG had an increasing effect on gastric contractility measured by bowel sound auscultation. Conclusions : BJ showed the possibility of inhibitory effects on gastric contraction. So, before administration, a gastric motility test should be recommended to prevent possible side effects in patients with gastric hypocontractility.