• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1583-1591
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• 2020

CRISPR/Cpf1 has emerged as a new CRISPR-based genome editing tool because, in comparison with CRIPSR/Cas9, it has a different T-rich PAM sequence to expand the target DNA sequence. Single-base editing in the microbial genome can be facilitated by oligonucleotide-directed mutagenesis (ODM) followed by negative selection with the CRISPR/Cpf1 system. However, single point mutations aided by Cpf1 negative selection have been rarely reported in Corynebacterium glutamicum. This study aimed to introduce an amber stop codon in crtEb encoding lycopene hydratase, through ODM and Cpf1-mediated negative selection; deficiency of this enzyme causes pink coloration due to lycopene accumulation in C. glutamicum. Consequently, on using double-, triple-, and quadruple-base-mutagenic oligonucleotides, 91.5-95.3% pink cells were obtained among the total live C. glutamicum cells. However, among the negatively selected live cells, 0.6% pink cells were obtained using single-base-mutagenic oligonucleotides, indicating that very few single-base mutations were introduced, possibly owing to mismatch tolerance. This led to the consideration of various target-mismatched crRNAs to prevent the death of single-base-edited cells. Consequently, we obtained 99.7% pink colonies after CRISPR/Cpf1-mediated negative selection using an appropriate single-mismatched crRNA. Furthermore, Sanger sequencing revealed that single-base mutations were successfully edited in the 99.7% of pink cells, while only two of nine among 0.6% of pink cells were correctly edited. The results indicate that the target-mismatched Cpf1 negative selection can assist in efficient and accurate single-base genome editing methods in C. glutamicum.

• BMB Reports
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• v.48 no.12
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• pp.643-644
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• 2015

In eukaryotes, small RNAs play important roles in both gene regulation and resistance to viral infection. Argonaute proteins have been identified as a key component of the effector complexes of various RNA-silencing pathways, but the mechanistic roles of Argonaute proteins in these pathways are not clearly understood. To address this question, we performed single-molecule fluorescence experiments using an RNA-induced silencing complex (core-RISC) composed of a small RNA and human Argonaute 2. We found that target binding of core-RISC starts at the seed region of the guide RNA. After target binding, four distinct reactions followed: target cleavage, transient binding, stable binding, and Argonaute unloading. Target cleavage required extensive sequence complementarity and accelerated core-RISC dissociation for recycling. In contrast, the stable binding of core-RISC to target RNAs required seed-match only, suggesting a potential explanation for the seed-match rule of microRNA (miRNA) target selection.

• Journal of radiological science and technology
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• v.39 no.4
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• pp.631-636
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• 2016

Hitherto, spatial resolution has commonly been evaluated by test patterns or phantoms built on some specific distances (from close to far) between two objects (or double targets). This evaluation method's shortcoming is that resolution is restricted to target distances of phantoms made for test. Therefore, in order to solve the problem, this study proposes and verifies a new method to efficiently test spatial resolution with a single target. For the research I used PSF and JND to propose an idea to measure spatial resolution. After that, I made experiments by commonly used phantoms to verify my new evaluation hypothesis inferred from the above method. To analyse the hypothesis, I used LabVIEW program and got a line pixel from digital image. The result was identical to my spatial-resolution hypothesis inferred from a single target. The findings of the experiment proves only a single target can be enough to relatively evaluate spatial resolution on a digital image. In other words, the limit of the traditional spatial-resolution evaluation method, based on double targets, can be overcome by my new evaluation one using a single target.

• Journal of the Korea Institute of Military Science and Technology
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• v.26 no.2
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• pp.122-128
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• 2023

The target must be located within the main-lobe of the antenna in order to measure the direction of the target by using sum-delta monopulse technique. The most common way if the target is located within the main-lobe is to compare the amplitude of the sum channel received signal with the delta channel received signal. However, in the case of the single-ring circular array antenna, it is difficult to apply the conventional method due to its structural limitation where antenna elements do not exist in the center of the array. In this paper, we proposed a novel method to identify whether a target is located within the main-lobe by appropriately adjusting the feeding amplitude of each element constituting the single-ring circular array antenna through the particle swarm optimization method. Simulation results showed that the proposed method can determine whether the target is located within the main-lobe of the single-ring circular array antenna.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.11
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• pp.5624-5638
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• 2016

A novel method based on Surf points is proposed to detect and lock-track single ground target in aerial videos. Videos captured by moving cameras contain complex motions, which bring difficulty in moving object detection. Our approach contains three parts: moving target template detection, search area estimation and target tracking. Global motion estimation and compensation are first made by grids-sampling Surf points selecting and matching. And then, the single ground target is detected by joint spatial-temporal information processing. The temporal process is made by calculating difference between compensated reference and current image and the spatial process is implementing morphological operations and adaptive binarization. The second part improves KALMAN filter with surf points scale information to predict target position and search area adaptively. Lastly, the local Surf points of target template are matched in this search region to realize target tracking. The long-term tracking is updated following target scaling, occlusion and large deformation. Experimental results show that the algorithm can correctly detect small moving target in dynamic scenes with complex motions. It is robust to vehicle dithering and target scale changing, rotation, especially partial occlusion or temporal complete occlusion. Comparing with traditional algorithms, our method enables real time operation, processing $520{\times}390$ frames at around 15fps.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.27 no.6
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• pp.539-544
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• 2016

This paper improves Sherman's two-pulse method for angle estimation of two unresolved targets in phase comparison monopulse radar. The proposed method provides the angle information with only a single-pulse measurement instead of two pulses. The proposed method can estimate a single-target angle by single-target indicator, in contrast with previous techniques. The accuracy of angle estimation for proposed method is demonstrated by simulations.

• IEMEK Journal of Embedded Systems and Applications
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• v.17 no.1
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• pp.67-76
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• 2022

Monostatic/Bistatic inverse synthetic aperture radar (ISAR) images are two-dimensional radar cross section (RCS) distributions of a target. When there are many targets in a single radar beam, ISAR images are generated with targets overlapped, so it is difficult to perform the targets identification using the trained database. In addition, it is inefficient to perform target identification using only single monostatic and bistatic ISAR images separately because each method has its own advantages and weaknesses. Therefore, this paper analyzes multiple targets identification performances using monostatic/bistatic ISAR images and proposes a method of identification through fusion of two ISAR images. To identify multiple targets, we use image combination technique using trained single target images. Simulation results show effectiveness of proposed method.

• Proceedings of the Korea Information Processing Society Conference
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• 2021.05a
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• pp.358-361
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• 2021

In order to improve the accuracy of small target detection more effectively, this paper proposes an improved single shot detector (SSD) target detection and recognition method based on cspdarknet53, which introduces lightweight ECA attention mechanism and Feature Pyramid Network (FPN). First, the original SSD backbone network is replaced with cspdarknet53 to enhance the learning ability of the network. Then, a lightweight ECA attention mechanism is added to the basic convolution block to optimize the network. Finally, FPN is used to gradually fuse the multi-scale feature maps used for detection in the SSD from the deep to the shallow layers of the network to improve the positioning accuracy and classification accuracy of the network. Experiments show that the proposed target detection algorithm has better detection accuracy, and it improves the detection accuracy especially for small targets.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.95.1-95.1
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• 2017

In this study, it has been tried to make the single Mo-Cu alloying targets with the Cu showing the best surface hardness that was determined by investigation on the coatings with the double target process. The single alloying targets were prepared by powder metallurgy methods such as mechanical alloying and spark plasma sintering. The nanocomposite coatings were prepared by reactive magnetron sputtering process with the single alloying targets in $Ar+N_2$ atmosphere. The microstructure changes of the Mo-Cu-N coatings with diverse Cu contents were investigated by using XRD, SEM and EDS. The mechanical properties of the coatings were evaluated by using nano-indentor, scratch test, and ball on disc methods. Especially, the coated samples were tested by using various lubricating oil to compare the property of anti wear-resistance. In this study, the nano-composite MoN-Cu coatings prepared using an alloying target was eventually compared with the coatings from the multiple targets.

• Journal of the Korea Society of Computer and Information
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• v.16 no.9
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• pp.155-162
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• 2011

It is using to incident angle estimation technique in order to target estimation in radar. This paper was estimated incident angle estimation for target using adaptive array incident angle and single look error incident angle estimation technique. We estimated signal incident angle of target to removal main lobe and side lobe to adaptive array incident angle technique. It is difficult to correctly target estimation because single look technique increase direction error of signal incident angle. In order to receive a desired target signal must be not almost look error between signal incident angle and look angle. we had decreased to occur a look error using delay time and single look condition to calculation a covariance when incident angle estimate. Through simulation, we show that the proposed incident angle estimation technique improves the performance of target estimation compared to previous method.