• Journal of Preventive Medicine and Public Health
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• 제28권4호
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• pp.863-873
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• 1995

This report describes a preperation and characterization of canine blood lead(Pb) standard reference material(SRM). Three adult beagle dogs(A, B, and C)were orally dosed with gelatin capsules containing $Pb(NO_3)_2$, equivalent to $10\sim80mg$ Pb/kg body weight. Blood was drawn 24 hours after the dose from the cephalic vein into lead free 500ml Pyrex beaker in which EDTA.K was contained as an anticoagulant. The amount of lead given to individual dog was varied arbitrarily. Three month later, 3 canine animals were orally dosed with lead secondarily to make mixed SRM(D1) which was mixed different concentrations of lead in bloods with A1, B1, and C1 in vitro. The SRMs for A, B, C, A1, B1, C1, and D1 were distributed 2ml each into more than 300 lead free bottles, and were stored in refregerator at $4^{\circ}C$. The amount of lead in canine whole blood samples were determined using a Varian 30A atomic absorption spectrophotometer(AAS) with a model GTA-96 graphite tube atomizer with D2 background correction and a Hitachi Z-8100 AAS with Zeeman background correction. The sensitivity and detection limits for lead determination of Varian 30A were $0.46{\mu}g/L,\;0.34{\mu}g/L,\;and\;0.56{\mu}g/L,\;0.14{\mu}g/L$ of Hitachi Z-8100, respectively. Day to day variations in determination of blood lead concentration in a certain sample were $31.11{\pm}1.36{\mu}g/100ml$ by Varian 30A, and $33.08{\pm}0.82{\mu}g/100ml$ by Hitachi Z-8100, showing the difference of 3% between the two results. At the blood lead concentrations of $56.31{\pm}1.98{\mu}g/100ml(A),\;40.89{\pm}0.80{\mu}g/100ml(B),\;59.01{\pm}1.38{\mu}g/100ml(C)$, the precisions of replicated measurements by AAS were 3.52%, 1.96%, and 2.34%, respectively. Coefficient variation(CV) of SRMs(A, B, and C) within a standard sample were ranged from 0.92% to 7.50%, and those between 5 standard samples were 1.21%, 2.64%, and 1.11%, respectively, showing inter-vial variation of $1{\mu}g/100ml$. Lead levels in SRMs during one month storage were unchanged. The overall recoveries were $89.6\sim100.4%,\;91.6\sim101.9%,\;90.3\sim100.0%$ for A, B, and C SRMs, means were $56.46{\pm}2.69{\mu}g/100ml,\;39.35{\pm}1.89{\mu}g/100ml,\;57.40{\pm}2.31{\mu}g/100ml$, and measurement ranges were$52.88{\pm}59.26{\mu}g/100ml,\;37.47{\pm}41.68{\mu}g/100ml,\;54.80{\pm}60.69{\mu}g/100ml$, respectively. Those results were laid within confidence limits values. The lead concentrations in the mixed sample(D1) stored over one month period were ranged from $32.76{\mu}g/100ml\;to\;33.54{\mu}g/100ml$, with CV ranging from 1.2% to 2.7%. The results were similiar to each of single samples(A1, B1, and C1) in respect of homogeneity and stability. Results of the mixed blood sample analysed after 1 month storage at $4^{\circ}C$ by four other laboratories(L1, L2, L3, L4) were similar with those of our laboratory($L5;31.18{\pm}0.24{\mu}g/100ml$, acceptable range by $CDC;25.18\sim37.18{\mu}g/100ml$), showing the concentrations of $25.91{\pm}1.19{\mu}g/100ml(L1),\;34.16{\pm}0.22{\mu}g/100ml(L2),\;35.68{\pm}0.85{\mu}g/100ml(L3),\;30.95{\pm}0.46{\mu}g/100ml(L4)$ in a each samples.

• 대한유전성대사질환학회지
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• 제13권2호
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• pp.111-119
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• 2013

목적: I형 뮤코다당증 (MPS I)은 ${\alpha}$-L-iduronidase 효소의 결핍으로 인하여 발생하는 리소좀 축적 질환으로, 광범위한 양상으로 다기관에 영향을 미친다. 저신장과 성장 속도의 감소는 MPS I의 중요한 특징이다. 본 연구에서는 효소 보충 요법이 MPS I 환자들의 성장에 미치는 효과에 대해 알아보기 위하여 단일 기관의 환자들을 대상으로 분석하였다. 방법: 2세에서 15세 사이에 효소 보충 요법을 시작하여 최소 3년 이상의 치료를 시행 받은 10명의 한국 MPS I 환자들의 키 측정치를 후향적으로 분석하였다. 효소 보충 요법 시작시의 평균 나이는 7년 7개월 이였으며, 남아는 6명, 여아는 4명 이였다. 키는 표준 편차(SDS)로 표현되었다. 효소 보충 요법 전과 후의 연간 성장 속도를 계산하였으며, 구분회귀모델을 이용하여 치료 전과 후의 키 z-score를 분석하였다. 표현형[(중증(Hurler) versus 경증(Hurler-Scheie, Scheie)]이 성장에 미치는 영향에 대해서는 개별 분석을 시행하였다. 결과: 효소 보충 요법 전 1년 동안의 연간 성장은 3.3 cm (z-score=-0.21) 였으며, 효소 보충 요법 후 1년, 2년, 3년에서는 각각 6.2 cm (z-score=0.17), 5.8 cm (z-score=0.07), 3.8 cm (z-score=-0.4)이였다. 회귀분석 결과, 효소 보충 요법 전에 비하여 치료 후 기울기에 유의한 호전을 보였다(기울기 차이=0.04; P=0.022). 중증과 경증 표현형 간의 치료 전(P=0.001)과 후(P<0.0001)의 기울기 차이는 통계적으로 유의하였으나, 표현형에 따라 분석하였을 때 통계적으로 유의한 차이는 보이지 않았다. 결론: MPS I 환자들의 키 성장에 있어 aldurazyme 효소 보충 요법이 긍정적인 효과를 미치는 것으로 보인다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2011년도 제36회 춘계학술대회논문집
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• pp.1-2
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• 2011

Hybrid rockets have lately attracted attention as a strong candidate of small, low cost, safe and reliable launch vehicles. A significant topic is that the first commercially sponsored space ship, SpaceShipOne vehicle chose a hybrid rocket. The main factors for the choice were safety of operation, system cost, quick turnaround, and thrust termination. In Japan, five universities including Hokkaido University and three private companies organized "Hybrid Rocket Research Group" from 1998 to 2002. Their main purpose was to downsize the cost and scale of rocket experiments. In 2002, UNISEC (University Space Engineering Consortium) and HASTIC (Hokkaido Aerospace Science and Technology Incubation Center) took over the educational and R&D rocket activities respectively and the research group dissolved. In 2008, JAXA/ISAS and eleven universities formed "Hybrid Rocket Research Working Group" as a subcommittee of the Steering Committee for Space Engineering in ISAS. Their goal is to demonstrate technical feasibility of lowcost and high frequency launches of nano/micro satellites into sun-synchronous orbits. Hybrid rockets use a combination of solid and liquid propellants. Usually the fuel is in a solid phase. A serious problem of hybrid rockets is the low regression rate of the solid fuel. In single port hybrids the low regression rate below 1 mm/s causes large L/D exceeding a hundred and small fuel loading ratio falling below 0.3. Multi-port hybrids are a typical solution to solve this problem. However, this solution is not the mainstream in Japan. Another approach is to use high regression rate fuels. For example, a fuel regression rate of 4 mm/s decreases L/D to around 10 and increases the loading ratio to around 0.75. Liquefying fuels such as paraffins are strong candidates for high regression fuels and subject of active research in Japan too. Nakagawa et al. in Tokai University employed EVA (Ethylene Vinyl Acetate) to modify viscosity of paraffin based fuels and investigated the effect of viscosity on regression rates. Wada et al. in Akita University employed LTP (Low melting ThermoPlastic) as another candidate of liquefying fuels and demonstrated high regression rates comparable to paraffin fuels. Hori et al. in JAXA/ISAS employed glycidylazide-poly(ethylene glycol) (GAP-PEG) copolymers as high regression rate fuels and modified the combustion characteristics by changing the PEG mixing ratio. Regression rate improvement by changing internal ballistics is another stream of research. The author proposed a new fuel configuration named "CAMUI" in 1998. CAMUI comes from an abbreviation of "cascaded multistage impinging-jet" meaning the distinctive flow field. A CAMUI type fuel grain consists of several cylindrical fuel blocks with two ports in axial direction. The port alignment shifts 90 degrees with each other to make jets out of ports impinge on the upstream end face of the downstream fuel block, resulting in intense heat transfer to the fuel. Yuasa et al. in Tokyo Metropolitan University employed swirling injection method and improved regression rates more than three times higher. However, regression rate distribution along the axis is not uniform due to the decay of the swirl strength. Aso et al. in Kyushu University employed multi-swirl injection to solve this problem. Combinations of swirling injection and paraffin based fuel have been tried and some results show very high regression rates exceeding ten times of conventional one. High fuel regression rates by new fuel, new internal ballistics, or combination of them require faster fuel-oxidizer mixing to maintain combustion efficiency. Nakagawa et al. succeeded to improve combustion efficiency of a paraffin-based fuel from 77% to 96% by a baffle plate. Another effective approach some researchers are trying is to use an aft-chamber to increase residence time. Better understanding of the new flow fields is necessary to reveal basic mechanisms of regression enhancement. Yuasa et al. visualized the combustion field in a swirling injection type motor. Nakagawa et al. observed boundary layer combustion of wax-based fuels. To understand detailed flow structures in swirling flow type hybrids, Sawada et al. (Tohoku Univ.), Teramoto et al. (Univ. of Tokyo), Shimada et al. (ISAS), and Tsuboi et al. (Kyushu Inst. Tech.) are trying to simulate the flow field numerically. Main challenges are turbulent reaction, stiffness due to low Mach number flow, fuel regression model, and other non-steady phenomena. Oshima et al. in Hokkaido University simulated CAMUI type flow fields and discussed correspondence relation between regression distribution of a burning surface and the vortex structure over the surface.

• 대한치과보철학회지
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• 제59권3호
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• pp.291-298
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• 2021

목적: 다섯 종류의 치간 세정 용품들을 이용하여, 지르코니아 크라운으로 수복된 치아의 치간공간에서 치면세균막 제거 효과의 임상적 차이를 살펴보는 것이다. 재료 및 방법: 하악 우측 제2소구치, 제1대구치 부위에 지대치를 형성한 모형을 제작하고, 두 지대치에 장착할 지르코니아 크라운을 각 10개씩 제작하였다. 인공 치면세균막을 제2소구치 원심면, 제1대구치 근심면에 도포한 후, 1인 실험자가 실제 구강 내에서 사용하는 모습을 재현하여 치간칫솔, 엔드터프트칫솔, 치실, 이지픽, 워터픽으로 군 당 10회 실험하였다. 디지털 카메라로 촬영된 이미지를 이용하여 제거된 양상 및 면적을 분석하였다. 통계 분석으로는 일요인 분산분석을 시행하였고, Scheffé 방법으로 사후검정 시행하였다 (P < .05). 결과: 치간 세정 용품에 따른 인공 치면세균막 제거 양상을 비교한 결과, 용품의 특성에 따라 제거되는 부위와 양상에 차이가 나타났다. 치실에서 변연 경계부를 포함하여 가장 넓은 면적이 제거되었다. 치간칫솔에서는 변연 경계부 위주로 제거가 되었으며, 이지픽은 치간칫솔보다 좁은 부위가 제거되었다. 엔드터프트칫솔은 협설면에서 인접면으로 이행되는 부분이 치간칫솔, 이지픽에 비해서 넓은 부위가 제거된 양상을 보였으나, 접촉점 직하방 부위는 거의 제거되지 않은 양상을 보였다. 워터픽에서는 거의 제거 되지 않은 양상을 보였다. 인공 치면세균막 제거율은 치실(69.47%), 엔드터프트칫솔(49.36%), 치간칫솔(44.20%), 이지픽(13.04%), 워터픽(0.59%) 순으로 나타났다. 결론: 지르코니아 크라운으로 수복된 치간공간에서의 치면세균막 제거율은 치실이 가장 높게 나타났으며, 변연경계부에서의 치면세균막 제거는 치간칫솔이 가장 효과적으로 나타났다.

• 한국환경생태학회지
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• 제33권4호
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• pp.472-480
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• 2019

도심지 내 미세먼지 저감 식재를 위한 식물 소재 선정 시에는 식물의 흡착 기능에 영향을 미치는 식물의 잎 모양, 질감, 수피의 형태 등 형태적 특성을 종합적으로 고려하여 선정하여야 한다. 그러나 지금까지 식물을 통한 미세먼지 저감에 대한 연구는 식물의 흡착 기능보다 흡수 기능에 대한 연구가, 실외식물보다 실내식물인 관엽식물을 대상으로 한 연구가 주로 진행되어 왔다. 특히, 미세먼지 저감 수종 선정 기준이 구체적이지 않아 미세먼지 저감 식재를 위한 식물 소재 선정 기준에 대한 연구가 필요한 실정이다. 본 연구는 퍼지다기준 의사결정법(Fuzzy MCDM)을 활용하여 미세먼지 저감에 영향을 미치는 8가지 지표 항목에 대한 우선순위를 도출하고 도심 내 미세먼지 저감 식재를 위한 수종 선정 기준을 마련하였다. 이를 위하여 미세먼지 관련 분야 전공자와 미세먼지 관련 연구 경험자들을 대상으로 설문 조사를 실시하였다. 설문 조사 분석 결과, 미세먼지 저감에 영향을 미치는 지표 항목 중 잎 면적과 수종의 종류가 가장 높은 순위를 나타냈다. 그리고 잎 표면의 거칠기, 수고, 성장 속도, 잎의 복잡성, 잎 가장자리 형태, 수피 특징 순서로 우선순위가 높게 나타났다. 잎 표면이 거친 수종을 선정할 경우에는 잎에 털이 있고, 광택이 나며, 왁스층이 있는 수종을 우선적으로 선정하는 것이 좋다. 잎의 형태를 고려할 경우, 단일 잎보다 3종 혹은 2종 잎과 손바닥 형태의 잎을 선정하고, 잎의 가장자리는 밋밋한 모양보다는 톱니 모양의 잎을 선별하여 공기 중의 미세먼지가 잎의 표면에 흡착되는 표면적 비율을 높아지게 할 필요가 있다. 수피의 특성을 고려할 경우에는 피목이나 무늬종보다는 코르크층이 있고 껍질이탈이나 갈라짐이 관찰되거나, 앞으로 관찰될 가능성이 높은 수종을 선정하는 것이 바람직하다. 본 연구는 도심 내 미세먼지 저감을 위한 식재계획 시 식물의 미세먼지 흡착 기능에 영향을 미치는 식물의 형태적 특성을 중심으로 식물 소재 선정 기준에 대한 우선순위를 제시하였다는 것에 의의가 있다. 본 연구에서 도출한 결과는 도심지 내 수목 식재 계획을 위한 수종 선정 시 기초 자료로서 활용될 수 있을 것이다.

• 한국국제농업개발학회지
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• 제30권4호
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• pp.277-284
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• 2018

본 연구는 돼지 농가의 생산성 향상 및 수익성 증대를 위한 분자 육종 기술에 적용할 유전자 마커를 개발하기 위해 수행되었다. 분석 결과에 따르면, 돼지의 간 조직을 이용하여 RNA-Sequencing을 수행한 결과 ECI2 유전자의 SNP를 발견하였고 발견된 SNP chr 7:g.2302809는 c.608로 608번째 C가 G로 변환되어 Threonine에서 Serine으로 아미노산이 치환되는 단일염기다형성임을 확인하였다. Berkshire 돼지 430으로 ECI2 유전자형과 육질 형질과의 연관성 분석 결과 도 체중, 적색도, 육즙 손실, 수분 함량 및 $pH_{24hr}$에서 유의성을 확인할 수 있었다. 그중 GG 유전자형은 $pH_{24hr}$에서 다른 유전자형에 비해 수치가 증가시키는 경향을 확인하였다. 성별에 따른 유전자형과 육질 형질과의 연관성은 거세돈에서 GG 유전자형이 육즙 손실의 감소와 $pH_{24hr}$에서 유의성이 확인되었고, 암퇘지의 GG 유전자형도 수분함량이 증가되었다. 따라서 ECI2 유전자의 GG 유전자형을 가진 돼지가 육질이 더 좋은 것으로 판단된다. 이러한 결과를 바탕으로 ECI2의 GG 유전자형을 고정시킨다면 육질이 우수한 돼지고기 생산이 가능할 것이다. 또한 ECI2 유전자를 이용하여 품종개량 및 조기 선발 기술에 바이오마커로 활용한다면 농가의 경쟁력 강화 효과에 도움이 될 것으로 사료된다.

• 환경생물
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• 제39권3호
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• pp.354-361
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• 2021

본 연구는 식물검역 분야에서 주요하게 사용되고 있는 메틸브로마이드 훈증제로 인해 발생하는 약해를 저감하기 위한 물질을 모델식물인 애기장대를 이용하여 스크리닝하였다. 사전연구를 통하여 메틸브로마이드 훈증제의 식물 독성 메커니즘으로 활성산소발생와 식물 성장 호르몬인 옥신의 식물체 내 분배억제효과가 발생하는 것을 바탕으로 하여, 약해 저감물질후보군으로 활성산소를 제거하는 역할을 하는 ROS scavenger 2종(NAC, GSH)과 옥신을 훈증제 처리 전 애기장대에 처리한 후 약해의 저감 정도를 육안평가와 더불어 관련 유전자의 발현을 확인하였다. 연구 결과 메틸브로마이드에 의해 유도된 약해는 옥신보다는 활성산소를 저감시키는 물질후보군들에서 약해 저감효과가 나타났다. 이 중 GSH을 이용하여 농도구배하여 전처리하였을 때, 5 mM GSH 전처리 후 메틸브로마이드 훈증시 약해 저감효과가 두드러졌다. GSH 전처리 시 식물체 내에 MBF1c와 HSP70 유전자 발현이 증가하는 것을 확인하였으며, 이는 메틸브로마이드 훈증으로 유도되는 약해를 방어하는 역할을 담당하였을 것이라고 평가된다. 따라서, 식물검역 훈증제 메틸브로마이드에 의해 발생하는 약해를 저감하는 데 GSH의 사용가능성을 평가하였으며, 이를 기반으로 다양한 식물체에 적용하여 수출입 시 약해로 인한 경제적 손실을 감소시킬 수 있기를 기대한다.

• 한국수자원학회논문집
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• 제54권8호
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• pp.629-641
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• 2021

본 연구에서는 2차원 수치모형(Nays2DH)을 적용하여 금강에 건설된 세종보를 중심으로 보 개방 후에 홍수량 변화에 의한 하도의 지형변화 과정을 분석하였다. 이를 위해 부등류, 부정류(단일사상), 연속홍수사상을 수문특성을 반영한 흐름조건으로 수치모의를 수행하였다. 부등류 수치모의 조건은 유황에 따른 정상유량으로 상류단 경계조건을 가정하였다. 부정류와 연속홍수사상의 경우에는 실제 홍수사상들로부터 정규 수문곡선 (normalized hydrograph)을 산정 한 후, 시나리오에 따른 첨두유량으로 정규 수문곡선을 재구성하여 상류단 유량으로 가정하였다. 본 연구에서는 지형학적 변화를 정량적으로 평가하기 위해 하상기복지수(BRI)를 산정하여 시간에 따른 하상변동을 분석하였으며, 연구지역의 항공사진과 수치모의 결과를 정성적으로 비교하였다. 부등류 수치모의 결과, 각 유황별 유량이 증가하면 하폭 대 수심의 비는 감소하고, 사주의 이동속도는 증가하였다. 하상기복지수는 초기에는 증가하지만, 시간이 증가함에 따라 변화량이 작아졌다. 또한 유량이 증가하면 하상기 복지수가 증가했다. 부정류 수치모의 결과 사주의 이동속도는 유량의 변화에 따라 감소했다. 또한 첨두홍수에 대한 지형적 반응에서 시간지체(time lag)가 발생했다. 즉, 부정류에서는 하상고의 변화는 수리학적 조건에 대하여 위상지연(Phase lag) 이 나타났다. 연속홍수사상 발생에 의한 부정류 수치모의 결과에서는 각각 첨두홍수 발생에 따른 사주의 이동속도는 홍수발생이 반복됨에도 불구하고 급격하게 감소했다. 또한 부정류 수치모의 결과와 마찬가지로 위상지연이 나타났으며 사주의 이동속도는 지수적으로 감소하는 특성을 보였다. 하상기복지수는 시간경과에 따라 증가하였으나, 첨두홍수가 연속으로 발생하였음에도 불구하고 하상기복지수의 증가율은 완만하였다. 본 연구를 통해 하천의 수문특성을 반영한 지형변화 과정을 수치모의를 수행하여 분석하였으며, 이를 통해 흐름특성에 따른 하상변동의 정량적인 예측모의를 현장에 적용할 수 있는 방법을 제시할 수 있을 것으로 판단된다.

• International Journal of Computer Science & Network Security
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• 제24권4호
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• pp.163-169
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• 2024

India is a developing nation and has come with comprehensive way in modernizing its reducing poverty, economy and rising living standards for an outsized fragment of its residents. The STEM (Science, Technology, Engineering, and Mathematics) education plays an important role in it. STEM is an educational curriculum that emphasis on the subjects of "science, technology, engineering, and mathematics". In traditional education scenario, these subjects are taught independently, but according to the educational philosophy of STEM that teaches these subjects together in project-based lessons. STEM helps the students in his holistic development. Youth unemployment is the biggest concern due to lack of adequate skills. There is a huge skill gap behind jobless engineers and the question arises how we can prepare engineers for a better tomorrow? Now a day's Industry 4.0 is a new fourth industrial revolution which is an intelligent networking of machines and processes for industry through ICT. It is based upon the usage of cyber-physical systems and Internet of Things (IoT). Industrial revolution does not influence only production but also educational system as well. IoT in academics is a new revolution to the Internet technology, which introduced "Smartness" in the entire IT infrastructure. To improve socio-economic status of the India students must equipped with 21st century digital skills and Universities, colleges must provide individual learning kits to their students which can help them in enhancing their productivity and learning outcomes. The major goal of this paper is to present a low cost, effective learning mechanism for STEM implementation using Raspberry Pi 3+ model (Single board computer) and Node Red open source visual programming tool which is developed by IBM for wiring hardware devices together. These tools are broadly used to provide hands on experience on IoT fundamentals during teaching and learning. This paper elaborates the appropriateness and the practicality of these concepts via an example by implementing a user interface (UI) and Dashboard in Node-RED where dashboard palette is used for demonstration with switch, slider, gauge and Raspberry pi palette is used to connect with GPIO pins present on Raspberry pi board. An LED light is connected with a GPIO pin as an output pin. In this experiment, it is shown that the Node-Red dashboard is accessing on Raspberry pi and via Smartphone as well. In the final step results are shown in an elaborate manner. Conversely, inadequate Programming skills in students are the biggest challenge because without good programming skills there would be no pioneers in engineering, robotics and other areas. Coding plays an important role to increase the level of knowledge on a wide scale and to encourage the interest of students in coding. Today Python language which is Open source and most demanding languages in the industry in order to know data science and algorithms, understanding computer science would not be possible without science, technology, engineering and math. In this paper a small experiment is also done with an LED light via writing source code in python. These tiny experiments are really helpful to encourage the students and give play way to learn these advance technologies. The cost estimation is presented in tabular form for per learning kit provided to the students for Hands on experiments. Some Popular In addition, some Open source tools for experimenting with IoT Technology are described. Students can enrich their knowledge by doing lots of experiments with these freely available software's and this low cost hardware in labs or learning kits provided to them.

• 유통과학연구
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• 제8권3호
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• pp.49-56
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• 2010

At the initial stage of Internet advertising, banner advertising came into fashion. As the Internet developed into a central part of daily lives and the competition in the on-line advertising market was getting fierce, there was not enough space for banner advertising, which rushed to portal sites only. All these factors was responsible for an upsurge in advertising prices. Consequently, the high-cost and low-efficiency problems with banner advertising were raised, which led to an emergence of keyword advertising as a new type of Internet advertising to replace its predecessor. In the beginning of 2000s, when Internet advertising came to be activated, display advertisement including banner advertising dominated the Net. However, display advertising showed signs of gradual decline, and registered minus growth in the year 2009, whereas keyword advertising showed rapid growth and started to outdo display advertising as of the year 2005. Keyword advertising refers to the advertising technique that exposes relevant advertisements on the top of research sites when one searches for a keyword. Instead of exposing advertisements to unspecified individuals like banner advertising, keyword advertising, or targeted advertising technique, shows advertisements only when customers search for a desired keyword so that only highly prospective customers are given a chance to see them. In this context, it is also referred to as search advertising. It is regarded as more aggressive advertising with a high hit rate than previous advertising in that, instead of the seller discovering customers and running an advertisement for them like TV, radios or banner advertising, it exposes advertisements to visiting customers. Keyword advertising makes it possible for a company to seek publicity on line simply by making use of a single word and to achieve a maximum of efficiency at a minimum cost. The strong point of keyword advertising is that customers are allowed to directly contact the products in question through its more efficient advertising when compared to the advertisements of mass media such as TV and radio, etc. The weak point of keyword advertising is that a company should have its advertisement registered on each and every portal site and finds it hard to exercise substantial supervision over its advertisement, there being a possibility of its advertising expenses exceeding its profits. Keyword advertising severs as the most appropriate methods of advertising for the sales and publicity of small and medium enterprises which are in need of a maximum of advertising effect at a low advertising cost. At present, keyword advertising is divided into CPC advertising and CPM advertising. The former is known as the most efficient technique, which is also referred to as advertising based on the meter rate system; A company is supposed to pay for the number of clicks on a searched keyword which users have searched. This is representatively adopted by Overture, Google's Adwords, Naver's Clickchoice, and Daum's Clicks, etc. CPM advertising is dependent upon the flat rate payment system, making a company pay for its advertisement on the basis of the number of exposure, not on the basis of the number of clicks. This method fixes a price for advertisement on the basis of 1,000-time exposure, and is mainly adopted by Naver's Timechoice, Daum's Speciallink, and Nate's Speedup, etc, At present, the CPC method is most frequently adopted. The weak point of the CPC method is that advertising cost can rise through constant clicks from the same IP. If a company makes good use of strategies for maximizing the strong points of keyword advertising and complementing its weak points, it is highly likely to turn its visitors into prospective customers. Accordingly, an advertiser should make an analysis of customers' behavior and approach them in a variety of ways, trying hard to find out what they want. With this in mind, her or she has to put multiple keywords into use when running for ads. When he or she first runs an ad, he or she should first give priority to which keyword to select. The advertiser should consider how many individuals using a search engine will click the keyword in question and how much money he or she has to pay for the advertisement. As the popular keywords that the users of search engines are frequently using are expensive in terms of a unit cost per click, the advertisers without much money for advertising at the initial phrase should pay attention to detailed keywords suitable to their budget. Detailed keywords are also referred to as peripheral keywords or extension keywords, which can be called a combination of major keywords. Most keywords are in the form of texts. The biggest strong point of text-based advertising is that it looks like search results, causing little antipathy to it. But it fails to attract much attention because of the fact that most keyword advertising is in the form of texts. Image-embedded advertising is easy to notice due to images, but it is exposed on the lower part of a web page and regarded as an advertisement, which leads to a low click through rate. However, its strong point is that its prices are lower than those of text-based advertising. If a company owns a logo or a product that is easy enough for people to recognize, the company is well advised to make good use of image-embedded advertising so as to attract Internet users' attention. Advertisers should make an analysis of their logos and examine customers' responses based on the events of sites in question and the composition of products as a vehicle for monitoring their behavior in detail. Besides, keyword advertising allows them to analyze the advertising effects of exposed keywords through the analysis of logos. The logo analysis refers to a close analysis of the current situation of a site by making an analysis of information about visitors on the basis of the analysis of the number of visitors and page view, and that of cookie values. It is in the log files generated through each Web server that a user's IP, used pages, the time when he or she uses it, and cookie values are stored. The log files contain a huge amount of data. As it is almost impossible to make a direct analysis of these log files, one is supposed to make an analysis of them by using solutions for a log analysis. The generic information that can be extracted from tools for each logo analysis includes the number of viewing the total pages, the number of average page view per day, the number of basic page view, the number of page view per visit, the total number of hits, the number of average hits per day, the number of hits per visit, the number of visits, the number of average visits per day, the net number of visitors, average visitors per day, one-time visitors, visitors who have come more than twice, and average using hours, etc. These sites are deemed to be useful for utilizing data for the analysis of the situation and current status of rival companies as well as benchmarking. As keyword advertising exposes advertisements exclusively on search-result pages, competition among advertisers attempting to preoccupy popular keywords is very fierce. Some portal sites keep on giving priority to the existing advertisers, whereas others provide chances to purchase keywords in question to all the advertisers after the advertising contract is over. If an advertiser tries to rely on keywords sensitive to seasons and timeliness in case of sites providing priority to the established advertisers, he or she may as well make a purchase of a vacant place for advertising lest he or she should miss appropriate timing for advertising. However, Naver doesn't provide priority to the existing advertisers as far as all the keyword advertisements are concerned. In this case, one can preoccupy keywords if he or she enters into a contract after confirming the contract period for advertising. This study is designed to take a look at marketing for keyword advertising and to present effective strategies for keyword advertising marketing. At present, the Korean CPC advertising market is virtually monopolized by Overture. Its strong points are that Overture is based on the CPC charging model and that advertisements are registered on the top of the most representative portal sites in Korea. These advantages serve as the most appropriate medium for small and medium enterprises to use. However, the CPC method of Overture has its weak points, too. That is, the CPC method is not the only perfect advertising model among the search advertisements in the on-line market. So it is absolutely necessary that small and medium enterprises including independent shopping malls should complement the weaknesses of the CPC method and make good use of strategies for maximizing its strengths so as to increase their sales and to create a point of contact with customers.