• Title/Summary/Keyword: Single-cell Measurement

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Adhesion Strength Measurement of Rabbit Knee Chondrocyte (연골세포 부착력 평가)

  • Lee Kwon-Yong;Park Sang-Guk;Shin Daehwan;Park Jong-Chul
    • Tribology and Lubricants
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    • v.21 no.5
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    • pp.236-240
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    • 2005
  • In order to prepare for the suitable surfaces of implants or medical devices, quantitative evaluation of adhesion between cells and biomaterials is essential. To better understand adhesion formation between cells and biomaterials, we used the cytodetachment technique which measures the adhesive force of a single cell through changing the, culture time and detachment speed. The results showed that the adhesive force could be affected by the culture time of cells on the surface of materials and the detachment speed. Moreover, there was a large discrepancy among the adhesion strength measured by similar techniques conducted on the different cells and substrates. It can be 'concluded that the variation of the force measurement technique can seriously alter the level of the force required to detach a cell on the surface of materials.

An implementation of fiber-optic sensors for impulse voltage and current measurement using a BSO and an YIG (BSO와 YIG를 이용한 임펄스 전압, 전류 측정용 광센서 구현)

  • 송재성;김영수
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.13 no.8
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    • pp.688-693
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    • 2000
  • In this paper an optical voltage sensor and an optical current sensor which can be used for the measurement of impulse voltage and current are implemented. BSO single crystal is utilized as a voltage sensor(Pockels effect cell). An rare earth doped YIG is used as a current sensor(Faraday effect cell). A new signal processing technique is adopted not only to avoid the influences o external optical fiber pertubations of transmitting optical fiber but also to improves the frequency response characteristics of the fiber-optic voltage and current sensors. Experimental results show that optical voltage sensor has maximum 2.5% error within the voltage range from 0V to 500V. and optical current sensor has maximum 2.5% error within the current range and that of optical current sensor is about 1.5% within temperature range from -2$0^{\circ}C$ to 6$0^{\circ}C$. The proposed optical sensors have good frequency response characteristics within the frequency range from DC to 10MHz.

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Measurement of the Metabolism of Energy Substrates in Single IVF-derived Bovine Embryos (소 체외수정란에 있어서 Non-invasive 방법에 의한 기질 대사량의 측정)

  • ;;S. Sugawara
    • Korean Journal of Animal Reproduction
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    • v.19 no.3
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    • pp.161-169
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    • 1995
  • To investigate the metabolism of various substrates in preimplantation bovine embryos, uptake of glucose and pyruvate, and lactate production were measured in single IVF-derived bovine embryos by a non-invasive method. When the embryos were incubated for 5 h in culture medium supplemented with 1 mM glucose and 0.4mM pyruvate as substrates at each developmental stage, glucose uptake was increased with more advanced developmental stages while pyruvate uptake was decreased. Total lactate producton of 2-cell embryos was significantly higher than that of blastocysts (p<0.05). Both of glucose uptake and lactate production in normal morulae produced in vitro was significantly high compared to the degenerated embryos(p<0.05). The results obtained in the study suggest that pyruvate as an exogenous substrate may be support in bovine embryos until 8-cell stage, whereas glucose may be effective as an energy source after morula stage. In addition, it was proven thatlactate was not effective as an energy source in preimplantation development of IVF-derived bovine embryos.

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Design of an Antireflection Coating for High-efficiency Superconducting Nanowire Single-photon Detectors

  • Choi, Jiman;Choi, Gahyun;Lee, Sun Kyung;Park, Kibog;Song, Woon;Lee, Dong-Hoon;Chong, Yonuk
    • Current Optics and Photonics
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    • v.5 no.4
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    • pp.375-383
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    • 2021
  • We present a simulation method to design antireflection coating (ARCs) for fiber-coupled superconducting nanowire single-photon detectors. Using a finite-element method, the absorptance of the nanowire is calculated for a defined unit-cell structure consisting of a fiber, ARC layer, nanowire absorber, distributed Bragg reflector (DBR) mirror, and air gap. We develop a method to evaluate the uncertainty in absorptance due to the uncontrollable parameter of air-gap distance. The validity of the simulation method is tested by comparison to an experimental realization for a case of single-layer ARC, which results in good agreement. We show finally a double-layer ARC design optimized for a system detection efficiency of higher than 95%, with a reduced uncertainty due to the air-gap distance.

A study on the fabrication of poly crystalline Si wafer by vacuum casting method and the measurement of the efficiency of solar cell

  • Lee, Geun-Hee;Lee, Zin-Hyoung
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.12 no.3
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    • pp.120-125
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    • 2002
  • Si-wafers for solar cells were cast in a size of $50{\times}46{\times}0.5{\textrm}{mm}^3$ by vacuum casting method. The graphite mold coated by BN powder, which was to prevent the reaction of carbon with the molten silicon, was used. Without coating, the wetting and reaction of Si melt to graphite mold was very severe. In the case of BN coating, SiC was formed in the shape of tiny islands at the surface of Si wafer by the reaction between Si-melt and carbon of the graphite mold on the high temperature. The grain size was about 1 mm. The efficiency of Si solar cell was lower than that of Si solar cell fabricated on commercial single and poly crystalline Si wafer. The reason of low efficiency was discussed.

Characterization of SiC-SiC Whisker Matrix Retaining Electrolyte in Phosphoric Acid Fuel Cell (인산형 연료전지용 SiC-SiC Whisker 전해질 매트릭스의 특성)

  • 윤기현;이현임;이근행;김창수
    • Journal of the Korean Ceramic Society
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    • v.29 no.8
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    • pp.587-592
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    • 1992
  • Sheets of SiC-SiC whisker maxed matrix were prepared from the mixed slurry of SiC whisker and SiC matrix by the rolling method. With the increase of SiC whisker, the pore size, the porosity and the phosphoric acid absorbency of the matrix were increased, while the bubble pressure was decreased. The activation energy for the transfer of H+ ion was decreased with the increase of mixing ratio of SiC whisker to the SiC matrix from the measurement of hydrogen ion conductivity. The activation energy was evaluated as 0.25 eV when the mixing ratio of SiC whisker to the SiC matrix was 1 : 2 and the activation energy was 0.16 eV for the 2 : 1 matrix. It means that SiC whisker matrix contributes to attain a better microstructure for the diffusion of hydrogen ion. From the measurement of single cell performance of matrix with various mixing ratio, it is concluded that if SiC-SiC whisker maxed matrix has a sufficient bubble pressure to prevent the crossover of H2 gas, the current density of a fuel cell is increased with the increase of acid absorbency of the matrix. Current density was improved from 140 mA/$\textrm{cm}^2$ for 0.25 mm thickness of matrix to 170 mA/$\textrm{cm}^2$ for the 0.20 mm one at 700 mV.

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Ultra-fast meseurement of partial discharge current due to conducting particle in Gas-insulated System (GIS) (가스절연기기 내부의 금속 이물질에 의한 초고속 부분방전량 및 전류 측정)

  • Park, Ki-Jun;Goo, Sun-Geun;Yoon, Jin-Yul
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.05c
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    • pp.160-164
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    • 2002
  • A special discharge cell containing a free conducting particle was used to generate the discharges in $SF_6$. The analog bandwidth of the measurement system exceeded 5 GHz. The measured signals were processed to compensate for the response of the measurement system. The discharge current rise time was in the range of 70 ps, while its full-width half-maximum was 120 ps. Single and double current pulses of positive and negative polarities were measured. Likewise, these pulses were compared with the measurement obtained using a conventional partial discharge detector of the IEC60270 type

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Growth and characterization of CdTe single crystal by vertical Bridgman method (수직 Bridgman 법에 의한 CdTe 단결정 성장과 특성)

  • Hong, Myung-Seok;Hong, Kwang-Joon
    • Journal of Sensor Science and Technology
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    • v.14 no.6
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    • pp.369-373
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    • 2005
  • High quality CdTe single crystal for the solar cell fabrication was grown by vertical Bridgman method. The etch pits patterns of (111) surfaces of CdTe etched by Nakagawa solution was observed the (111)A compesed of Cd atoms with typical triangle etch pits of pyramid mode. From the photoluminescence measurement on (111)A, we observed free exciton ($E_{x}$) existing only high quality crystal and neutal acceptor bound exciton ($A^{0}$,X) having very strong peak intensity. Then, the full width at half maximum and binding energy of neutral acceptor bound exciton were 7 meV and 5.9 meV, respectively. By Haynes rule, an activation enery of impurity was 59 meV. Therefore, the origins on impurity level acting as a neutral acceptor were associated Ag or Cu elements.

Uncertainty Analysis and Improvement of an Altitude TestFacility for Small Jet Engines

  • Jun, Yong-Min;Yang, In-Young;Kim, Chun-Taek;Yang, Soo-Seok;Lee, Dae-Sung
    • International Journal of Aeronautical and Space Sciences
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    • v.5 no.1
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    • pp.46-56
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    • 2004
  • The verification and improvement of the measurement uncertainty have beenperformed in the altitude test facility for small gas turbine engines, which was built atthe Korea Aerospace Research Institute (KARI) in October 1999. This test is performedwith a single spool turbojet engine at several flight conditions. This paper discussesthe evaluation and validation process for the measurement uncertainty improvements usedin the altitude test facility. The evaluation process, defined as tests before the facilitymodification, shows that the major contnbutors to the measurement uncertainty are theflow meter discharge coefficient, the inlet static and total pressures, the cell pressureand the fuel flow rate. The measurement uncertainty is focused on the primary parametersof the engine performance such as airflow rate, thrust and specific fuel consumption (SFC).The validation process, defined as tests after the facility modification, shows that themeasurement uncertainty, in seal level condition, is tmproved to the acceptable level throughthe facility modification. In altitude test conditions, the measurement uncertainties arenot improved as much as the uncertainty in sea level condition.

The Study of Growth and Characterization of CuGaSe$_2$ Sing1e Crystal Thin Films for solar cell by Hot Wall Epitaxy (HWE(Hot Wall Epitaxy)에 의한 태양 전지용 박막성장과 특성에 관한 연구)

  • 홍광준
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.07a
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    • pp.237-242
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    • 2001
  • The stochiometric mix of evaporating materials for the CuGaSe$_2$ single crystal thin films was prepared from horizontal furnace. To obtain the single crystal thin films, CuGaSe$_2$ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were 610$^{\circ}C$ and 450$^{\circ}C$, respectively The crystalline structure of single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). From the photocurrent spectrum by illumination of perpendicular light on the c-axis of the CuGaSe$_2$ single crystal thin film, we have found that the values of spin orbit splitting Δ So and the crystal field splitting ΔCr were 91 meV and 249.8 meV at 20 K, respectively. From the Photoluminescence measurement on CuGaSe$_2$ single crystal thin film, we observed free excition (Ex) existing only high quality crystal and neutral bound exiciton (D$^{\circ}$,X) having very strong peak intensity. Then, the full-width-at-half-maximum(FWHM) and binding energy 7f neutral acceptor bound excision were 8 meV and 35.2 meV, respectivity. By Haynes rule, an activation energy of impurity was 355.2 meV

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