• Title/Summary/Keyword: Single-blind method

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SNP Marker Development for Purity Test of Oriental Melon and Melon (멜론 및 참외 순도 검정을 위한 SNP 마커 개발 및 F1 종자 순도 검정)

  • An, Song-Ji;Kwon, Jin-Kyung;Yang, Hee-Bum;Choi, Hye-Jeong;Jeong, Hee-Jin;Kim, Yong-Jae;Choi, Gyung-Ja;Kang, Byoung-Cheorl
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.397-406
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    • 2010
  • Field screening method has been commonly used for purity test of $F_1$ hybrid seeds in melon and oriental melon. However, as this method takes a lot of time and cost, molecular marker-based purity test is necessary. To develop molecular markers for purity test, thirty pairs of SNP (single nucleotide polymorphism) primers were obtained from melon EST sequences, and 10 polymorphic markers showing HRM (high resolution melting) polymorphisms between parents of two melon cultivars and one oriental melon cultivar were selected. Blind tests were performed to validate usefulness of the selected markers for purity test. Blind test results showed that HRM genotypes were matched with the expected identity of individual sample, $F_1$ hybrid, male or female parents. Three HRM-based SNP markers were converted to CAPS markers for general use which is favor to breeders. We expect that SNP markers developed in this study will be useful for purity test of $F_1$ hybrid seeds in melon and oriental melon.

Comparison of Glass Particle Contamination according to Method of Ampule Cutting and Needle Aspiration (유리앰풀 개봉 관련 요인에 따른 주사용액 내 유리조각 혼입 정도 비교)

  • Park, Jeong-Sook;Oh, Hyae-Ryeung;Seo, Bo-Hyae;Bhang, Jung-Hee
    • Journal of Korean Academy of Nursing
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    • v.36 no.6
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    • pp.1033-1041
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    • 2006
  • Purpose: Glass particle contamination of the contents of single-dose glass ampules can occur upon opening. Different aspiration techniques, different sizes of needles, different sizes of ampules, and different cutting methods were studied to determine if they had any effect on glass particle contamination. Method: Different aspiration techniques(with filter, without filter), different sizes of needles(18G, 25G), different sizes of ampules(2ml, 20m1), and different cutting methods(with cotton, without cotton) were evaluated. Method: Twenty ampules were randomly assigned in each group. Three slides containing glass particles for each ampule were made and counted under a microscope by 3 study blind persons. Result: The number of glass particle contamination is much less when using a filter rather than without a filter. The number of glass particle contamination is much less when using a 25G needle rather than on 18G needle. The number of glass particle contamination is much less when using 2ml ampules rather than 20m1 ampules. The number of glass particle contamination is much less when using cotton rather than without cotton. Conclusion: It was shown that using a filter, a small size needle, smaller sized ampules and using cotton when cutting the ampule will decrease the risk of parenteral injection of glass particles.

Comparison of pain relief in soft tissue tumor excision: anesthetic injection using an automatic digital injector versus conventional injection

  • Hye Gwang Mun;Bo Min Moon;Yu Jin Kim
    • Archives of Craniofacial Surgery
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    • v.25 no.1
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    • pp.17-21
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    • 2024
  • Background: The pain caused by local anesthetic injection can lead to patient anxiety prior to surgery, potentially necessitating sedation or general anesthesia during the excision procedure. In this study, we aim to compare the pain relief efficacy and safety of using a digital automatic anesthetic injector for local anesthesia. Methods: Thirty-three patients undergoing excision of a benign soft tissue tumor under local anesthesia were prospectively enrolled from September 2021 to February 2022. A single-blind, randomized controlled study was conducted. Patients were divided into two groups by randomization: the experimental group with digital automatic anesthetic injector method (I-JECT group) and the control group with conventional injection method. Before surgery, the Amsterdam preoperative anxiety information scale was used to measure the patients' anxiety. After local anesthetic was administered, the Numeric Pain Rating Scale was used to measure the pain. The amount of anesthetic used was divided by the surface area of the lesion was recorded. Results: Seventeen were assigned to the conventional group and 16 to the I-JECT group. The mean Numeric Pain Rating Scale was 1.75 in the I-JECT group and 3.82 in conventional group. The injection pain was lower in the I-JECT group (p< 0.01). The mean Amsterdam preoperative anxiety information scale was 11.00 in the I-JECT group and 9.65 in conventional group. Patient's anxiety did not correlate to injection pain regardless of the method of injection (p= 0.47). The amount of local anesthetic used per 1 cm2 of tumor surface area was 0.74 mL/cm2 in the I-JECT group and 2.31 mL/cm2 in the conventional group. The normalization amount of local anesthetic was less in the I-JECT group (p< 0.01). There was no difference in the incidence of complications. Conclusion: The use of a digital automatic anesthetic injector has shown to reduce pain and the amount of local anesthetics without complication.

Rear Vehicle Detection Method in Harsh Environment Using Improved Image Information (개선된 영상 정보를 이용한 가혹한 환경에서의 후방 차량 감지 방법)

  • Jeong, Jin-Seong;Kim, Hyun-Tae;Jang, Young-Min;Cho, Sang-Bok
    • Journal of the Institute of Electronics and Information Engineers
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    • v.54 no.1
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    • pp.96-110
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    • 2017
  • Most of vehicle detection studies using the existing general lens or wide-angle lens have a blind spot in the rear detection situation, the image is vulnerable to noise and a variety of external environments. In this paper, we propose a method that is detection in harsh external environment with noise, blind spots, etc. First, using a fish-eye lens will help minimize blind spots compared to the wide-angle lens. When angle of the lens is growing because nonlinear radial distortion also increase, calibration was used after initializing and optimizing the distortion constant in order to ensure accuracy. In addition, the original image was analyzed along with calibration to remove fog and calibrate brightness and thereby enable detection even when visibility is obstructed due to light and dark adaptations from foggy situations or sudden changes in illumination. Fog removal generally takes a considerably significant amount of time to calculate. Thus in order to reduce the calculation time, remove the fog used the major fog removal algorithm Dark Channel Prior. While Gamma Correction was used to calibrate brightness, a brightness and contrast evaluation was conducted on the image in order to determine the Gamma Value needed for correction. The evaluation used only a part instead of the entirety of the image in order to reduce the time allotted to calculation. When the brightness and contrast values were calculated, those values were used to decided Gamma value and to correct the entire image. The brightness correction and fog removal were processed in parallel, and the images were registered as a single image to minimize the calculation time needed for all the processes. Then the feature extraction method HOG was used to detect the vehicle in the corrected image. As a result, it took 0.064 seconds per frame to detect the vehicle using image correction as proposed herein, which showed a 7.5% improvement in detection rate compared to the existing vehicle detection method.

Brick Path Recognition Using Image Shape Pattern and Texture Feature (영상의 형태 패턴과 텍스처 특징을 이용한 보도블록의 인식방법)

  • Woo, Byung-Seok;Yang, Sung-Min;Jo, Kang-Hyun
    • Journal of Korea Multimedia Society
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    • v.15 no.4
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    • pp.472-484
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    • 2012
  • Raised or plain block is widely used for the pedestrian's safe passage. The insincere construction, insufficient maintenance and obstacle overlaid on the pavement cause pedestrian's accidents. This paper proposes a method to detect brick path by analyzing the shape pattern and texture feature of brick located in visible distance for a safe passage. A brick appears to a regular type because of its specific shape which repeats with its sized gap and its type varies according to the surrounding environment or use. This paper shows a method which extracts the shape pattern by analyzing single surface polygon and its frequency appearing in road area. The shape pattern is used to detect similar shape regions. Some regions are not detected because extraneous substances or chopped bricks distort the original shape. This problem can be solved by analyzing the texture feature vector. The analyzed vector of the previously detected regions yields the Gaussian distribution. This value in each undetected region is computed and checked whether it's satisfied with Gaussian distribution or not. The satisfied region is detected as the brick path. The experiment was performed with the various type's bricks to recognize so that the results showed as accurate as 95.9% in average.

The effect of wall squat exercise according to the difference in the support surface on the muscle thickness and balance of the trunk (지지면 차이에 따른 월 스쿼트 운동이 몸통 근두께와 균형에 미치는 영향)

  • Jeong, Jin Gyu;Park, Jae Cheol
    • Journal of Korean Physical Therapy Science
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    • v.29 no.1
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    • pp.64-72
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    • 2022
  • Background: The purpose of this study is to investigate the effect of wall squat exercise according to the difference in the support surface on the thickness change of external oblique, internal oblique and transverse abdominalis of the trunk muscles and the change in distance of center of pressure when the eyes are opened and closed. Design: Randomized controlled trial. Methods: The subjects were 26 healthy adults, 18 males and 8 females. The composition of each group using the single-blind method was 13 people in the unstable side wall squat exercise group and 13 people in the stable side wall squat exercise group. Experimental measurements were divided into before, 3 weeks, and 6 weeks after the experiment, and changes in muscle thickness and balance were confirmed. Muscle thickness was measured using ultrasinic, and static balance change was measured using Bio-rescue. Results: There were significant differences in the thickness changes of external oblique, internal oblique and transverse abdominalis according to the wall squat exercise method by period and in the interaction between the period and the group (p<0.05). As a result of the post-hoc analysis, there was a significant difference in the change between the two groups in external oblique muscle after 6 weeks. And there was a significant difference in the distance of the pressure center between eyes open and closed eyes (p<0.05). Conclusion: In terms of instability, wall squat exercise was positive for changes in trunk muscle thickness. It suggests the possibility of using it for trunk muscle strengthening training in the future.

Effects of Sequential Application of Superficial Cold and Heat on Pain, Patient Satisfaction with Pain Control, Comfort Level and Subjective Response after Spine Surgery (순차적 냉·온 요법이 척추 수술 후 통증과 통증 조절 만족도, 안위, 주관적 반응에 미치는 효과)

  • Kim, Jeoung Hee;Lhim, Seung Chul;Roh, Sung Woo;Lee, Sun Jin;Ko, Young Mi;Kim, Yeo Ok;Shin, Yong Soon
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.23 no.2
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    • pp.184-193
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    • 2016
  • Purpose: The aims of the current study were to evaluate the effects of superficial cold and heat after spine surgery on pain, satisfaction with pain control and comfort level, and to identify subjective responses and adverse effects. Methods: A prospective, single-blind, randomized controlled trial was utilized. The intervention group (n=36) received superficial cooling until the wound drain was removed and thereafter followed by superficial heating until discharge, while the control group (n=34) received only superficial cooling until wound drain was removed. Data were collected from August 4 to November 11 2014. Results: There was significant difference in pain according to time within groups (F=71.87, p<.001). However, we found no difference in pain between groups. The intervention group reported higher patient satisfaction with pain control (4 vs 3, z=-2.83, p=.005) and higher comfort level (5 vs 4, z=-4.12, p<.001) than the control group. Conclusion: Results indicate that sequential application of superficial cold and heat is a useful method in clinical practice for management of pain after spine surgery.

4% lidocaine versus 4% articaine for inferior alveolar nerve block in impacted lower third molar surgery

  • Boonsiriseth, Kiatanant;Chaimanakarn, Sittipong;Chewpreecha, Prued;nonpassopon, Natee;Khanijou, Manop;Ping, Bushara;Wongsirichat, Natthamet
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.17 no.1
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    • pp.29-35
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    • 2017
  • Background: No study has compared lidocaine with articaine, each at a concentration of 4% and combined with epinephrine. The purpose of this study was to compare the effectiveness of 4% lidocaine with that of 4% articaine, with a concentration of 1:100,000 epinephrine added to each, in an inferior alveolar nerve block for surgery on impacted lower third molars. Method: This study was conducted at the Faculty of Dentistry, Mahidol University in Bangkok, Thailand. The randomized, single-blind, comparative split-mouth study was carried out in patients with symmetrically impacted lower third molars, as identified on panoramic radiographs. Each patient underwent surgery for the removal of the lower third molars by the same surgeon under local anesthesia at two separate visits, 3 weeks apart. The onset and duration of local anesthesia, intra-operative pain, surgical duration, and number of additional anesthetics administered were recorded. Results: The subjective and objective onset of action for the local anesthetics showed statistically significant differences (P < 0.05). However, the intra-operative pain, surgical duration, duration of local anesthesia, and number of additional anesthetics administered did not show statistically significant differences. Conclusion: The use of 4% articaine for the inferior alveolar nerve block was clinically more effective in the onset of subjective and objective anesthesia as compared with the use of 4% lidocaine. Based on the pain scores from the visual analogue scale, 4% lidocaine provided more analgesia during the procedure, and patients noted less intra-operative pain than with 4% articaine; however, the difference was not clinically significant.

Comparison of Whole versus Split-Dose PEG Solution for Colonoscopy Preparation on Patient Compliance, Quality of Bowel Cleansing, and Endoscopist's Satisfaction (대장내시경 정결제 복용방법에 따른 대상자 순응도, 대장정결 효과, 및 시술자 만족도)

  • Park, Keum-Mi;Kim, Myung Hee;Hwang, Sun Kyung;Kim, Dong Hee;Kim, Ju Sung
    • Korean Journal of Adult Nursing
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    • v.19 no.2
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    • pp.237-247
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    • 2007
  • Purpose: This study was a randomized single-blind trial of whole versus split-dose PEG solutions for colonoscopy preparation to compare the patient compliance, quality of bowel cleansing, and endoscopist's satisfaction. Methods: The participants were recruited from outpatients who planned to receive colonoscopy of C hospital in Busan. Sixty participants were randomly assigned to receive either a spit-dose group(n=30) consuming $2{\ell}$ of PEG solution twice, or a whole-dose group(n=30), consuming $4{\ell}$ of PEG solution once. These participants completed the questionnaire to assess their compliance before colonoscopy. The quality of bowel cleansing was assessed using the Ottawa Scale with the endoscopist who was blinded to the type of preparation, and their satisfaction by using VAS. Results: The participants who did not completely consume $4{\ell}$ of PEG solution were less in split-dose than in whole-dose group (0% vs 13.3%). The split-dose group complained less about abdominal pain(t=2.644, p=0.009) and abdominal bloating(t=2.802, p=0.013) with a statistical significance. For the quality of bowel preparation, there were no significant differences in the bowel cleansing scores and the endoscopist's satisfaction between two groups. Conclusion: Colonic preparation with split-dose of PEG solution could be a more useful method for better patient compliance, with no significant impact on bowel cleansing quality.

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Multi-aperture Photometry Pipeline for DEEP-South Data

  • Chang, Seo-Won;Byun, Yong-Ik;Kim, Myung-Jin;Moon, Hong-Kyu;Yim, Hong-Suh;Shin, Min-Su;Kang, Young-Woon
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.56.2-56.2
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    • 2016
  • We present a multi-aperture photometry pipeline for DEEP-South (Deep Ecliptic Patrol of the Southern Sky) time-series data, written in C. The pipeline is designed to do robust high-precision photometry and calibration of non-crowded fields with a varying point-spread function, allowing for the wholesale search and characterization of both temporal and spatial variabilities. Our time-series photometry method consists of three parts: (i) extracting all point sources with several pixel/blind parameters, (ii) determining the optimized aperture for each source where we consider whether the measured flux within the aperture is contaminated by unwanted artifacts, and (iii) correcting position-dependent variations in the PSF shape across the mosaic CCD. In order to provide faster access to the resultant catalogs, we also utilize an efficient indexing technique using compressed bitmap indices (FastBit). Lastly, we focus on the development and application of catalog-based searches that aid the identification of high-probable single events from the indexed database. This catalog-based approach is still useful to identify new point-sources or moving objects in non-crowded fields. The performance of the pipeline is being tested on various sets of time-series data available in several archives: DEEP-South asteroid survey and HAT-South/MMT exoplanet survey data sets.

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