• Molecules and Cells
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• v.46 no.2
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• pp.120-129
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• 2023

Recent technical advances have enabled unbiased transcriptomic and epigenetic analysis of each cell, known as "single-cell analysis". Single-cell analysis has a variety of technical approaches to investigate the state of each cell, including mRNA levels (transcriptome), the immune repertoire (immune repertoire analysis), cell surface proteins (surface proteome analysis), chromatin accessibility (epigenome), and accordance with genome variants (eQTLs; expression quantitative trait loci). As an effective tool for investigating robust immune responses in coronavirus disease 2019 (COVID-19), many researchers performed single-cell analysis to capture the diverse, unbiased immune cell activation and differentiation. Despite challenges elucidating the complicated immune microenvironments of chronic inflammatory diseases using existing experimental methods, it is now possible to capture the simultaneous immune features of different cell types across inflamed tissues using various single-cell tools. In this review, we introduce patient-based and experimental mouse model research utilizing single-cell analyses in the field of chronic inflammatory diseases, as well as multi-organ atlas targeting immune cells.

• Nuclear Engineering and Technology
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• v.48 no.5
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• pp.1140-1153
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• 2016

Since the inception of nuclear power as a commercial energy source, safety has been recognized as a prime consideration in the design, construction, operation, maintenance, and decommissioning of nuclear power plants. The release of radioactivity to the environment requires the failure of multiple safety systems and the breach of three physical barriers: fuel cladding, the reactor cooling system, and containment. In this study, nuclear reactor containment pressurization has been modeled in a large break-loss of coolant accident (LB-LOCA) by programming single-cell and multicell models in MATLAB. First, containment has been considered as a control volume (single-cell model). In addition, spray operation has been added to this model. In the second step, the single-cell model has been developed into a multicell model to consider the effects of the nodalization and spatial location of cells in the containment pressurization in comparison with the single-cell model. In the third step, the accident has been simulated using the CONTAIN 2.0 code. Finally, Bushehr nuclear power plant (BNPP) containment has been considered as a case study. The results of BNPP containment pressurization due to LB-LOCA have been compared between models, final safety analysis report, and CONTAIN code's results.

• Molecules and Cells
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• v.27 no.4
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• pp.491-492
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• 2009

We present the result of our research on the tumorigenic ability of single cell clones isolated from an aggressive murine breast cancer cell line in a matched allografting mouse model. Tumor formation is basically dependent on the cell numbers injected per location. We argue that in vivo tumor formation from single cell clones, isolated in vitro from cancer cell lines, may not provide conclusive evidence to disprove the cancer stem cell (CSC) theory without additional data.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.1
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• pp.56-63
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• 2009

Cell migration is one of the essential mechanisms responsible for complex biological processes. Intensive researches have begun to elucidate the mechanisms and search intriguing conditions for efficient control of cell migration. One general mechanism that is widely applicable for cells including Escherichia coli, amoebae and endothelial cell is chemotaxis. The single cell study for bacterial chemotaxis has an advantage over studies with the population of cells in providing a clearer observation of cell migration, which leads to more accurate assessments of chemotaxis. In this paper, we propose a three-dimensional model considering a single bacterium to study its chemotaxis. The semi-implicit Fourier spectral method is applied for high efficiency and numerical stability. The simulation results reveal rich dynamics of cell migration and provide quantitative assessments of bacterial chemotaxis with various chemoattractant gradient fields.

• Molecules and Cells
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• v.44 no.3
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• pp.127-135
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• 2021

Since the introduction of RNA sequencing (RNA-seq) as a high-throughput mRNA expression analysis tool, this procedure has been increasingly implemented to identify cell-level transcriptome changes in a myriad of model systems. However, early methods processed cell samples in bulk, and therefore the unique transcriptomic patterns of individual cells would be lost due to data averaging. Nonetheless, the recent and continuous development of new single-cell RNA sequencing (scRNA-seq) toolkits has enabled researchers to compare transcriptomes at a single-cell resolution, thus facilitating the analysis of individual cellular features and a deeper understanding of cellular functions. Nonetheless, the rapid evolution of high throughput single-cell "omics" tools has created the need for effective hypothesis verification strategies. Particularly, this issue could be addressed by coupling cell engineering techniques with single-cell sequencing. This approach has been successfully employed to gain further insights into disease pathogenesis and the dynamics of differentiation trajectories. Therefore, this review will discuss the current status of cell engineering toolkits and their contributions to single-cell and genome-wide data collection and analyses.

• Structural Engineering and Mechanics
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• v.55 no.5
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• pp.953-964
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• 2015

A model has been proposed that can predict the ultimate torsional strength of single-box multi-cell reinforced concrete box girder under combined loading of bending, shear and torsion. Compared with the single-cell box girder, this model takes the influence of inner webs on the distribution of shear flow into account. According to the softening truss theory and thin walled tube theory, a failure criterion is presented and a ultimate torsional strength calculating procedure is established for single-box multi-cell reinforced concrete box girder under combined actions, which considers the effect of tensile stress among the concrete cracks, Mohr stress compatibility and the softened constitutive law of concrete. In this paper the computer program is also compiled to speed up the calculation. The model has been validated by comparing the predicted and experimental members loaded under torsion combined with different ratios of bending and shear. The theoretical torsional strength was in good agreement with the experimental results.

• Proceedings of the KIPE Conference
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• 2015.07a
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• pp.373-374
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• 2015

This paper proposes a dynamic thermo-electric model that links electrical parameters with thermal parameters. In this model, the irradiance and ambient temperature are used to calculate the cell temperature based on a four-layer model that includes the PV cell and surround materials. The calculated cell temperature is then used in the electrical model to accurately adjust the PV electrical characteristics. Dynamic PV characteristics, parallel capacitive and series inductive components, are added to the conventional single-diode model. The results show the effectiveness of this model rather than other conventional models of a PV panel.

• 제어로봇시스템학회:학술대회논문집
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• 1991.10b
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• pp.1627-1632
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• 1991

A compact type LDPE autoclave reactor is analyzed with respect to the effects of the initiator feed concentration and the rate of heat transfer by employing the mixing-cell model with backflow. Singularity theory is applied for the single-cell model so that one can construct all the possible bifurcation diagrams. Since the single-cell model may not be adequate for the actual reactor, a two-cell model is also treated to predict the dynamic behavior of the reactor. As the rate of heat transfer increases, various multiplicity patterns and oscillatory motions are found. Apparently, the monomer conversion can be substantially increased with proper he-at removal and initiator supplement scheme. For this, however, the complex dynamic features accompanied must be taken into consideration in the reactor design.

• 전기의세계
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• v.28 no.8
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• pp.66-71
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• 1979

This paper describes electrical analysis of the information processing of the nervous system. A general-purpose electrical neuronal model for simulating the electrical activity in a single nerve cell and in small groups of nerve cell has constructed. This model consists of two basic electronic modules to represent respectively a "cell body" and an "axon (with synapses)", together with various related appurtenances. The primary advantages of this method are; holistic view, actual physical representation of various electrical activities in a single nerve cell, display of the activity of all nerve cells flexibility with respect to network parameters. Moreover, this model can effectively help push forward our general ability to explore and conceptualize the electrical activity of interconnected networks of nerve cell behaving in concert. Also, this electronic module technique is the best of various means for this task of realistic representation of aggregates of neurons.gregates of neurons.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1638-1642
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• 2008

Cell migration is one of the essential mechanisms responsible for complex biological processes. Intensive researches have begun to elucidate the mechanisms and search intriguing conditions for efficient control of cell migration. One general mechanism which is widely applicable for cells including neutrophil, Escherichia coli and endothelial cell is chemotaxis. Especially, understanding the chemotactic mechanics of cell crawling has important implications for various medical and biological applications. The single cell study for chemotaxis has an advantage over studies with the population of cells in providing a clearer observation of cell migration, which leads to more accurate assessments of chemotaxis. In this paper, we propose a three-dimensional model considering a single crawling cell to study its chemotaxis. The semi-implicit Fourier spectral method is applied for high efficiency and numerical stability. The simulation results reveal rich dynamics of cell.