• Title/Summary/Keyword: Single domain

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Functional Analysis of the Heptasequence SPTSPTY in the Transcriptional Activation Domain of Rat Nuclear Factor 1-A

  • Hwang, Jung-Su;Son, Kyung-No;Rho, Hyune-Mo;Kim, Ji-Young
    • BMB Reports
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    • v.32 no.5
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    • pp.468-473
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    • 1999
  • Nuclear Factor 1 (NF1) proteins are a family of transcriptional factors consisting of four different types: NF1-A, -B, -C, and -X. Some NF1 transcription factors contain a heptasequence motif, SPTSPSY, which is found as a repeat sequence in the carboxy terminal domain (CTD) of the largest subunit of RNA polymerase II. A similar heptasequence, SPTSPTY, is contained in rat liver NF1-A at a position between residues 469 and 475. In order to investigate the roles of the individual amino acids of the heptasequence of rat liver NF1-A in transcriptional activation, we systematically substituted single and multiple amino acid residues with alanine residue(s) and evaluated the transcriptional activities of the mutated NF1-A. Substitution of a single amino acid reduced transcriptional activity by 10 to 30%, except for the proline residue at position 473, whose substitution with alanine did not affect transcriptional activity. However, changes of all four serine and threonine residues to alanine or of the tyrosine residue along with the serine residue at position 469 to alanine reduced the activity to almost background levels. Our results indicate that multiple serine and threonine residues, rather than a single residue, may be involved in the modulation of the transcriptional activities of the factor. Involvement of the tyrosine residue is also implicated.

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Single crystals growth and properties of $LiNbO_{3}$ doped with MgO or ZnO : (I) Single crystals growth and their defect structure (MgO 또는 ZnO를 첨가한 $LiNbO_{3}$ 단결정 성장 및 특성 : (I) 단결정 성장 및 결함구조)

  • Cho, Hyun;Shim, Kwang-Bo;Auh, Keun-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.3
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    • pp.368-376
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    • 1996
  • $LiNbO_{3}$ single crystals (undoped, 5 mol% MgO-doped and 5 mol% ZnO-doped) were grown by the floating zone method which has the characteristics of a compositional homogeneity and uniform distribution of the dopants. The optimum growth condition was established experimentally and the defect structures such as domain structure, dislocation structure, slip band, and microtwins were characterized using a microscopic method.

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Achievable Bit Rate Comparison of Cyclic Prefixed CI/OFDM System and Single Carrier System (Cyclic Prefixed CI/OFDM 시스템과 단일반송파 시스템의 ABR 비교 분석)

  • Zheng, Hui;Hwang, Jae-Ho;Hwnag, Dae-Geun;Kim, Jae-Moung
    • Journal of Satellite, Information and Communications
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    • v.5 no.1
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    • pp.6-16
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    • 2010
  • Since OFDM system suffers from high peak-to average power ratio(PAPR) drawbacks, more energy has been converted to seek for a new substitutable system which can maintain OFDM system's inherent virtues while avoid its defects. Consequently, a new multicarrier system called as CI/OFDM system has been proposed which applied carrier interferometry(CI) code to OFDM system. Due to its low PAPR advantage and orthogonal property, it has received more and more attention. Simultaneously, an old technique called single carrier(SC) system has retaken its attractions for the same purposes. This paper analyzes two cyclic prefixed transmission schemes variants of OFDM system: 1.carrier interferometry-Orthogonal Frequency-Division Multiplexing (CI/OFDM); 2. Cyclic prefixed single carrie(CP-SC) with frequency domain equalization. We compare the achievable bit rate transmission of the two systems in terms of signal to noise ratio(SNR) by mathematical derivation. We demonstrated that CI/OFDM achieves a bit higher transmission bit rate to that of the CP-SC with frequency domain equalizer.

Single Carrier Frequency Domain Equalization in 3-slot Based Amplify-and-Forward Relaying Network for Shadow Area (음영 지역을 위한 3-슬롯 기반의 AF 방식 중계기 네트워크에서의 단일 반송파 주파수 대역 등화 기법)

  • Won, Hui-Chul
    • Journal of Korea Society of Industrial Information Systems
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    • v.17 no.7
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    • pp.9-16
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    • 2012
  • In order to extend cell coverage and to cope with shadow areas, a relay-assisted wireless communications system has been widely studied. In this paper, we propose new equalization method for single carrier (SC) frequency domain equalizer (FDE) in amplify-and-forward (AF) relaying multi-path networks to improve the performance at shadow areas. The performance of SC-FDE system in 3-slot based multi-path networks can be improved considerably with the diversity gain which we obtain by equalizing the combined signal from relays by means of the minimum mean square error (MMSE) criteria. We find the weighting coefficients of maximum ratio combining (MRC) and the tap coefficients of MMSE equalizer for SC-FDE in AF relaying multi-path networks. Simulation results show that the proposed system considerably outperforms the conventional SC-FDE system.

Sensing Domain and Extension Rate of a Family B-Type DNA Polymerase Determine the Stalling at a Deaminated Base

  • Kim, Yun-Jae;Cha, Sun-Shin;Lee, Hyun-Sook;Ryu, Yong-Gu;Bae, Seung-Seob;Cho, Yo-Na;Cho, Hyun-Soo;Kim, Sang-Jin;Kwon, Suk-Tae;Lee, Jung-Hyun;Kang, Sung-Gyun
    • Journal of Microbiology and Biotechnology
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    • v.18 no.8
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    • pp.1377-1385
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    • 2008
  • The uracil-sensing domain in archaeal family B-type DNA polymerases recognizes pro-mutagenic uracils in the DNA template, leading to stalling of DNA polymerases. Here, we describe our new findings regarding the molecular, mechanism underpinning the stalling of polymerases. We observed that two successive deaminated bases were required to stall TNA1 and KOD1 DNA polymerases, whereas a single deaminated base was enough for stalling Pfu DNA polymerase, in spite of the virtually identical uracil-sensing domains. TNA1 and KOD1 DNA polymerases have a much higher extension rate than Pfu DNA polymerase; decreasing the extension rate resulted in stalling by TNA1 and KOD1 DNA polymerases at a single deaminated base. These results strongly suggest that these polymerases require two factors to stop DNA polymerization at a single deaminated base: the presence of the uracil-sensing domain and a relatively slow extension rate.