• Structural Engineering and Mechanics
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• v.78 no.5
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• pp.599-610
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• 2021

Early detection of small concrete crack or reinforcement corrosion is necessary for Structural Health Monitoring (SHM). Global vibration based methods are advantageous over local methods because of simple equipment installation and cost efficiency. Among vibration based techniques, FRF based methods are preferred over modal based methods. In this study, a new coupled method using frequency response function (FRF) and proper orthogonal modes (POM) is proposed by using the dynamic characteristic of a damaged beam. For the numerical simulation, wave finite element (WFE), coupled with traditional finite element (FE) method is used for effectively incorporating the damage related information and faster computation. As reported in literature, hybrid combination of wave function based wave finite element method and shape function based finite element method can addresses the mid frequency modelling difficulty as it utilises the advantages of both the methods. It also reduces the dynamic matrix dimension. The algorithms are implemented on a three-dimensional reinforced concrete beam. Damage is modelled and studied for two scenarios, i.e., crack in concrete and rebar corrosion. Single and multiple damage locations with different damage length are also considered. The proposed methodology is found to be very sensitive to both single- and multiple- damage while being computationally efficient at the same time. It is observed that the detection of damage due to corrosion is more challenging than that of concrete crack. The similarity index obtained from the damage parameters shows that it can be a very effective indicator for appropriately indicating initiation of damage in concrete structure in the form of spread corrosion or invisible crack.

• Toxicological Research
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• v.23 no.1
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• pp.25-31
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• 2007

To evaluate the protective effect of Houttuynia cordata on hydrogen peroxide-induced oxidative DNA damage in HepG2 cell line, we used an alkaline single-cell gel electrophoresis (SCGE; comet assay). The DNA damage was analyzed by tail moment (TM) and tail length (TL), which used markers of DNA strand breaks in SCGE. The $100{\mu}g/ml$ of methanolic extract of Houttuynia cordata root showed significant protective effects (p < 0.01) against hydrogen peroxide-induced DNA damage in HepG2 cells and increased cell viability against hydrogen peroxide. The results of this study indicate that Houttuynia cordata root methanol extract acts as a potential antioxidant, and exhibits potential anticancer properties, which may provide a clue to find applications in new pharmaceuticals for oxidative stability.

• Molecular & Cellular Toxicology
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• v.1 no.1
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• pp.32-39
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• 2005

Polycyclic aromatic hydrocarbons (PAHs) are an important class of environmentally prevalent xenobiotics that exert complex effects on the biological system and characterized as probably carcinogenic materials. Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T-and B lymphocytes, spleens (T/B-cell), bone marrow, and livers of mouse exposed to mixture of PAHs (Benzo(a)pyrene, Benzo(e)pyrene, Fluoranthene, Pyrene) at dose of 400, 800, or 1600 mg/kg body weight for 2 days. DNA damage of the cells purified from mice was increased in dose dependent manner. In the blood cells and organs, DNA damage was also discovered to vary directly with PAHs. Especially T-cells had been damaged more than B-cell. Plasma proteomes were separated by 2-dimensional electrophoresis with pH 4-7 ranges of IPG Dry strips and many proteins showed significant up-and -down expressions with the dose dependent manner. Of these, significant 4 spots were identified using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. Identified proteins were related to energy metabolism and signal transduction.

• Journal of the Korean Society of Fashion and Beauty
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• v.5 no.1 s.12
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• pp.71-77
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• 2007

This study attempted to comparative analysis about the physical and morphological damage degrees of hair according to the bleaching methods by $H_2O_2$ concentration. As a result of the changes in the physical characteristics of the hair according to the deceleration operation methods by 3-6-9% and 9% $H_2O_2$ concentration, the intensity of tension considerably decreased but the elongation was increased. And the more the damage of hair, the larger the degree of change. Regarding the method of deceleration operation, the 3-6-9% deceleration operation method was more damaging than the 9% single deceleration operation method. In the change of the characteristics of the form of the hair, the control group of the 5-level deceleration hair generally showed a smooth external appearance and regular and closely overlapped epidermis and undamaged cuticle were observed. But in the 3-6-9% deceleration operation method and 9% single deceleration operation method, damages such as the irregular form of the edge of the epidermis and the unclear boundary between the epidermis occurred.

• YAKHAK HOEJI
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• v.54 no.6
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• pp.423-428
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• 2010

The aim of this study was to evaluate the in vivo effect of galangin and the 70% ethanolic extract of Alpinia officinarum (AO) toward $KBrO_3$-induced DNA and chromosomal damage in mice. Galangin and AO inhibited the formation of 8-hydroxy-2'-deoxyguanosine (8-OH2'dG) as an indicator of DNA oxidative damage in the liver cell. Galangin and AO showed the inhibitory effect on the formation of DNA single strand break in the splenocyte by single cell gel electrophoresis (SCGE) assay and also inhibited micronucleated reticulocyte (MNRET) formation of peripheral blood in tail blood of mice. Vit-E revealed antigenotoxic effects in DNA and chromosome levels, but galangin was more potent active compound compare to vit-E under our experimental conditions. The results suggest that the extract of Alpinia officinarum containing galangin can modify the oxidative DNA and chromosomal damage and may act as chemopreventive agent against oxidative stress in vivo.

• Journal of the Korea Institute of Military Science and Technology
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• v.21 no.3
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• pp.279-287
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• 2018

This study investigated applicability of the fast running model for damage assessment of underground structures by ground shock. For this reason, the fast running model that consists of two main models such as the ground shock generation and propagation model and the underground structural damage assessment model was developed. The ground shock generation and propagation model was programed using theoretical formula and empirical formula introduced in TM5-855-1(US army manual). The single degree of freedom model of structural components was utilized to predict structural dynamic displacements which are used as index to assess damage level of components. In order to confirm the feasibility of the developed fast running model, underground structural dynamic displacements estimated from the fast running model were compared to displacements obtained from the finite element analysis.

• Steel and Composite Structures
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• v.28 no.6
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• pp.719-734
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• 2018

Prior to the introduction of modern seismic guidelines, it was a common practice to provide straight bar anchorage for beam bottom reinforcement of gravity load designed building. Exterior joints with straight bar anchorages for beam bottom reinforcements are susceptible to sudden anchorage failure under load reversals and hence require systematic seismic upgradation. Hence in the present study, an attempt is made to upgrade exterior beam-column sub-assemblage of a three storied gravity load designed (GLD) building with single steel haunch. Analytical formulations are presented for evaluating the haunch forces in single steel haunch retrofit. Influence of parameters that affect the efficacy and effectiveness of the single haunch retrofit are also discussed. The effectiveness of the single haunch retrofit for enhancing seismic performance of GLD beam-column specimen is evaluated through experimental investigation under reverse cyclic loading. The single steel haunch retrofit had succeeded in preventing the anchorage failure of beam bottom bars of GLD specimen, delaying the joint shear damage and partially directing the damage towards the beam. A remarkable improvement in the load carrying capacity of the upgraded GLD beam-column sub-assemblage is observed. Further, a tremendous improvement in the energy dissipation of about 2.63 times that of GLD specimen is observed in the case of upgraded GLD specimen. The study also underlines the efficacy of single steel haunch retrofit for seismic upgradation of deficient GLD structures.

• Journal of Korean Society of Steel Construction
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• v.9 no.1 s.30
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• pp.95-105
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• 1997

Structures subjected to strong seismic excitation may undergo inelastic deformation cycles. The resulting cumulative fatigue damage process reduces the ability of structures and components to withstand seismic loads. Yet, the present earthquake resistance design methods focus mainly on the maximum displacement ductility, ignoring the effect of the cyclic responses. The damage parameters closely related to the cumulative damage need to be properly reflected on the aseismic design methods. In this study, two cumulative damage assessment methods derived from the plastic fatigue theory are investigated. The one is based on the hysteretic ductility amplitude, and the other is based on the dissipated hysteretic energy. Both methods can consider the maximum ductility and the cyclic behavior of structural response. The validity of two damage methods has been examined for single degree of freedom structures with various natural frequencies against two different earthquake excitations.

• Structural Engineering and Mechanics
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• v.33 no.3
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• pp.325-337
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• 2009

Structural damage detection, damage localization and severity estimation of jacket platforms, based on calculating modal strain energy is presented in this paper. In the structure, damage often causes a loss of stiffness in some elements, so modal parameters; mode shapes and natural frequencies, in the damaged structure are different from the undamaged state. Geometrical location of damage is detected by computing modal strain energy change ratio (MSECR) for each structural element, which elements with higher MSECR are suspected to be damaged. For each suspected damaged element, by computing cross-modal strain energy (CMSE), damage severity as the stiffness reduction factor -that represented the ratios between the element stiffness changes to the undamaged element stiffness- is estimated. Numerical studies are demonstrated for a three dimensional, single bay, four stories frame of the existing jacket platform, based on the synthetic data that generated from finite element model. It is observed that this method can be used for damage detection of this kind of structures.

• Biomedical Science Letters
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• v.7 no.4
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• pp.181-190
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• 2001

This study was designed to investigate the effects of reactive oxygen species (ROS) on DNA stability in human spermatozoa. To verify human spermatozoa were incubated with xanthine-xanthine oxidase (X 100$\mu$M-XO 50 mlU ~ 400 mIU), $H_2O_2$ (125 $\mu$M ~ 1 mM), sodium nitroprusside (SNP 0.1 $\mu$M ~ 100 $\mu$M) or lymphocyte. Otherwise, spermatozoa were incubated under low $O_2$ (5%) condition. Damage of sperm DNA was analyzed by single cell electrophoresis (Comet assay) and flow cytometry after acridine orange staining. In the presence of ROS, there was increase in DNA damage. The rate of DNA single strand breakage (9.0$\pm$1.0% ~ 46.0$\pm$4.6%) and DNA fragmentation (7.51$\pm$1.0% ~ 29.5$\pm$4.6%) were similar regardless of the kinds of ROS and exposure time. DNA damage in the lower $O_2$ condition (5%) was lower than ambient $O_2$ condition (20%). Taken together, it suggested that sperm DNA might be damaged by ROS. In the presence of ROS, increase in DNA damage and chromatin instability was obvious in spite of short exposure. Although present study reconfirmed that sperm incubation in the low concentration of ROS have the benefit m the induction of capacitation and Ah, the increase in DNA damage by ROS and possible genetic problem should be considered before the human trials.