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Sea, wind, or bird: Origin of Fagus multinervis (Fagaceae) inferred from chloroplast DNA sequences (엽록체 염기서열을 통한 너도밤나무(너도밤나무과)의 기원 추론)

  • Oh, Sang-Hun
    • Korean Journal of Plant Taxonomy
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    • v.45 no.3
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    • pp.213-220
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    • 2015
  • To elucidate the origin and patterns of establishment of insular plants on Ulleungdo Island, maternally inherited chloroplast DNA, which is useful for tracing seed movements, was used. Fagus multinervis, an endemic species that dominated broadleaf deciduous forests on Ulleungdo Island, is an excellent model for such a study. To understand the diversity and spatial distribution of the chloroplast haplotypes of F. multinervis, nucleotide sequences of the psbA-trnH region were determined from 144 individuals sampled throughout the island. Results of a phylogenetic analysis of the region with close relatives of F. multinervis suggest that F. multinervis is sister to a clade of F. japonica and F. engleriana. No haplotype variation was found within F. multinervis. This remarkably low cpDNA haplotype diversity is in contrast to the findings of previous allozyme studies of F. multinervis populations that showed high genetic diversity on Ulleungdo Island. Repeated colonization during the early stage of establishment via birds that migrated from a source area where the F. multinervis cpDNA haplotype was geographically structured may have resulted in the observed pattern of haplotype diversity. Alternatively, long-distance dispersal of seeds of the progenitor of F. multinervis via birds or typhoons to Ulleungdo may have been a single event, whereas the immigration of pollen from the mainland likely occurred frequently. Comparative phylogeographic studies of other species endemic to Ulleungdo Island and their close relatives on the neighboring mainland are necessary for a more complete understanding of the evolution of the island's native species.

Development on an Automatic Calibration Module of the SWMM for Watershed Runoff Simulation and Water Quality Simulation (유역유출 및 수질모의에 관한 SWMM의 자동 보정 모듈 개발)

  • Kang, Taeuk;Lee, Sangho
    • Journal of Korea Water Resources Association
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    • v.47 no.4
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    • pp.343-356
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    • 2014
  • The SWMM (storm water management model) has been widely used in the world and is a watershed runoff simulation model used for a single event or a continuous simulation of runoff quantity and quality. However, there are many uncertain parameters in the watershed runoff continuous simulation module and the water quality module, which make it difficult to use the SWMM. The purpose of the study is to develop an automatic calibration module of the SWMM not only for watershed runoff continuous simulation, but also water quality simulation. The automatic calibration module was developed by linking the SWMM with the SCE-UA (shuffled complex evolution-University of Arizona) that is a global optimization algorithm. Estimation parameters of the SWMM were selected and search ranges of them were reasonably configured. The module was validated by calibration and verification of the watershed runoff continuous simulation model and the water quality model for the Donghyang Stage Station Basin. The calibration results for watershed runoff continuous simulation model were excellent and those for water quality simulation model were generally satisfactory. The module could be used in various studies and designs for watershed runoff and water quality analyses.

Distribution of Available Soil Phosphorus and Effect of Phosphorus Application on Appearance of Algal Waterbloom and Rice Yield in Honam Area (호남지역(湖南地域) 논 토양(土壤)의 유효인산함량(有效燐酸含量) 및 인산시비(燐酸施肥)에 따른 괴불발생(發生)과 수량(收量))

  • Yoo, Chul-Hyun;Choi, Jeong-Weon;Shin, Bog-Woo;Lee, Sang-Bokg;Jeong, Ji-Ho;Kim, Seong-Jo;Han, Seong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.3
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    • pp.270-274
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    • 1996
  • To estimate the suitable method of phosphorus application in puddled-soil drill seeding of rice, available phosphorus, appearance of algal water bloom, and rice yield were investigated in paddy soil of Honam area from 1994 to 1995. Available phosphorus content with different regions was the highest in the east-west inland region of Noryeong mountain range and the inland region of Honam area and it was the lowest in the Charyeong south plains region. Available phosphorus content was the highest in the alluvial plains. and it was the lowest in dilluvial terrace. Frations of phosphorus in Jeonbug series were high in the order of Ca-P, Fe-P, and Al-P, respectively. Algal water bloom occurred highly in 100% and 70% basal applications before seeding, and slightly in no-fertilizer and 100% application of 3-leaf stage in puddled-soil drill seeding of rice. Suitable amount of phosphorus application was 21kg/ha in the single cropping paddy field of Fluvio-marine plain, and rice yield was increased 6% at 50% basal application.

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The Effects of Radix aconiti on the Contraction of Rat's Spinal Cord Injury (부자(附子)의 경구투여(經口投與)가 RAT의 척수손상(脊髓損傷)에 미치는 영향(影響) 및 비교(比較))

  • Lee Geon-Mok;Kim Kyung-Sik;Lee Kang-Chang
    • Journal of Acupuncture Research
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    • v.15 no.2
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    • pp.105-116
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    • 1998
  • Purpose In this study, We observed the recovery process in the ability to move in the hind limbs of the rats whose spinal cord injuries were treated by Radix acouniti(RA). The purpose of this study is to see the effects of Radix acouniti(RA) water extract on the contraction of rat's spinal cord injury. Procedure First, the rats were subjected to hemisectional spinal cord injuries by a scalpel blade. Those rats, then, were divided into three groups: Sham operated rats group and the experimental group, which received the Radix acouniti(RA) water extract, and the control group, which had no treatment. Their recovery in the ability to walk was observed by the Open Field Test (OFT) for 14 days after the injuries. Method The OFT was applied at four points: the hip, knee, ankle joint, and the tail. Each joint was given a movement rating of from 0 to 3, depending on the amount of movement. A movement rating of 0 designates no movement, a 1 designates slight movement, a 2 designates increased movement, and a 3 designates active movement. Slight movement is defined as a joint displaying less than or equal to 30% of that joint range, increased movement is displaying less than or equal to 60% of that joint's range, and active movement is greater than or equal to 90% of that joint's range. Tail movement is also graded on a scale of 0 to 3. A rating of 0 indicates that the tail is down 100% of the time, one of 1 indicates that the tail is down more than 10%, one of 2 shows that the tail is down less than 50% but more than 10% of the time, and one of 3 shows that the tail is down less than 10% of the time. All four ratings were added together and then averaged to arrive at a single score. Results The sham group which did not go through spinal cord injuries showed near normal results on all 3 joints and tail from right after the operation, which one would expect. The RA oral application group showed more effective recovery of movememt function than the control group around 4 days after the spinal cord injuries. However, after 14 days, both groups displayed almost the same degree of movement recovery. The results of this study are summarized as follows: 1. After 14days the spinal cord injuries, movement was recovered in sham operated group, control group, and experimented group in the hip, the knee, the tail and then the ankle of rats, in that order. 2. Around 7 days after the spinal cord injuries, the experimental group proved the effectiveness of the therapy in terms of movement recovery. 3. The level of ALT, ALP, AST in RA treated group was slightly increased. 4. The level of BUN and creatinine in RA treated group was slightly increased. The above results indicate that RA therapy at an early stage can bring about better movement recovery in patients with spinal cord injuries from traffic accidents or industrial disasters. But there is apparent side effect of RA on clinical, therefore the study on this should be continued.

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A CLINICAL STUDY ON THE ANATOMICAL SITE SURVIVAL RATE IN INTRAORAL SQUAMOUS CELL CARCINOMA (구강내 부위별 편평 상피암종의 생존율에 관한 임상 연구)

  • Kim, Kyung-Wook;Lee, Tae-Hee
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.29 no.5
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    • pp.315-322
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    • 2003
  • Background : Important factors to determine treatment method and prognosis of oral cancer are anatomical site, tumor size, metastatic lesion, histologic cell differenciation and microvascular invasion. Anatomical site has great effect to oral cancer patient's survival rate because each site's accessibility and lymph node metastasis is different but this factor was't studied much than other factors. Patients and Methods : 228 patients with squamous cell carcinoma of common primary sites(Mandible, Maxilla, Floor of Mouth and Tongue) in oral cavity who were diagnosed in the Korea Cancer Center Hospital from January 1989 to December 1999, were clinically studied and analyzed on survival rate. Results : 1. Survival rates of each anatomical sites were Tongue(36.8%), Mandible(33.3%), Maxilla(28.7%) and Floor of Mouth(24.5%). Survival rates difference between Tongue and Floor of Mouth has significance(p<0.05). 2. Survival rates for early cancer of each site were Maxilla(100%), Mandible(57.1%), Tongue(54.2%) and Floor of Mouth(46.7%). Survival rates difference between Maxilla and Floor of Mouth has significance(p<0.05). 3. Survival rates by surgery method of each site were Maxilla(60.6%), Tongue(56.9%), Mandible(44.8%) and Floor of Mouth(26.3%). Survival rates difference between Maxilla and Floor of Mouth has significance(p<0.05). 4. Survival rates by radiation or chemo method of each site were Floor of Mouth(23.5%), Mandible(20.0%), Maxilla(9.5%), and Tongue(9.1%). Survival rates difference between each site doesn't have significance(p>0.05). 5. In advance stage, Survival rates by single therapy of each site were Tongue(33.6%), Mandible(23.5%), Floor of Mouth(16.7%), Maxilla(0%), and Survival rates difference between Maxilla and Tongue has significance (p<0.05). Survival rates by combination therapy of each site were Mandible(38.1%), Maxilla(30.0%), Floor of mouth(18.2%), Tongue(12.5%), and Survival rates difference between Mandible and Tongue has significance(p<0.05). Conclusion : Survival rate of tongue is higher than the other sites, early detection of oral cancer can increase survival rate at any site and combination therapy is the most effetive method, especially at maxilla.

Dietary Value of Three Benthic Diatom Species on Haliotis discus hannai Larvae (북방전복 Haliotis discus hannai 유생에 대한 3종 부착 규조류의 먹이효율)

  • Park, Se Jin;Hur, Sung Bum
    • The Korean Journal of Malacology
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    • v.29 no.2
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    • pp.91-96
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    • 2013
  • Although the method of seedling production of Haliotis discus hannai is well known, the optimum benthic diatom species as a live food at early larval stage are not fully developed. In this study three Pennales diatom species, Caloneis schroederi, Rhaphoneis sp., and Cocconeis californica were examined on settlement, metamorphosis, survival, and growth of Haliotis discus hannai larvae. The larvae fed Raphoneis sp. or C. californica showed high settlement rate with 80-82% within 48 hrs, which was significantly higher than those fed C. schroederi or mixed diets with three diatom species. The larvae fed the former microalgal species also showed higher metamorphosis rate with 32-34% than the latter species with 10-12% within 4 days. With regard to survival and growth of the larvae, single diet with Rhaphoneis sp. or C. californica had better dietary value than the mixed diets for the early larvae of H. discus hannai.

10MHz/77dB dynamic range CMOS linear-in-dB variable gain amplifiers (10MHz/77dB 다이내믹 영역을 가진 선형 가변 이득 증폭기)

  • Cha, Jin-Youp;Yeo, Hwan-Seok;Kim, Do-Hyung;Burm, Jin-Wook
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.8
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    • pp.16-21
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    • 2007
  • CMOS variable gain amplifier (VGA) IC designs for the structure monitoring systems of the telemetries were developed. A three stage cascaded VGA using a differential amplifier and a linear-in-dB controller is presented. A proposed VGA is a modified version of a conventional VGA such that the gain is controlled in a linear-in-dB fashion through the current ratio. The proposed VGA circuit introduced in this paper has a dynamic range of 77 dB with 1.5 dB gain steps. It also achieved a gain error of less than 1.5 dB over 77 dB gain range. The VGA can operate up to 10MHz dissipating 13.8 mW from a single 1.8 V supply. The core area of the VGA fabricated in a Magnachip $0.18{\mu}m$ standard CMOS process was about $430{\mu}m{\times}350{\mu}m$. According to measurement results, we can verify that the proposed method is reasonable with regard to the enhancement of dynamic range and the better linear-in-dB characteristics.

Levels and Related Factors of Airborne Fungi in Microbial and Chemistry Laboratories in Universities (일부 대학교 미생물실험실 및 화학실험실에서의 진균 분포 및 관련인자)

  • Hwang, Sung-Ho;Jo, Hyun-Woo;Park, Dong-Uk;Yoon, Chung-Sik;Ryu, Kyong-Nam;Ha, Kwon-Chul
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.20 no.1
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    • pp.41-46
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    • 2010
  • The purpose of this study is to assess the level of fungi concentration in the university laboratories in Seoul, Korea, and to investigate factors contributing to these concentrations. The samples were taken from three spots in each laboratory; the top of sink, the center of laboratory, and the front of ventilation system, i.e fume hood at the chemical laboratory and clean bench/biosafety cabinet at the microbial laboratory. Air samples were collected using the single-stage Anderson sampler (Quick Take 30) at a flow rate of 28.3 l/min for 5 min on nutrient media in Petri-dishes located on the impactor. Fifty-two air samples were collected from 19 different laboratories (13 microbiology laboratories, 6 chemistry laboratories) in the university, and concentrations of airborne fungi showed no significant difference (p>0.05) between microbiology and chemistry laboratory, and also no significant difference at three locations (sink, center, front of ventilation system) in microbiology and chemistry laboratories. Average concentrations of fungi in 19 laboratories ranged from 7 to 459 cfu/$m^3$, with an overall Geometric Mean of 52 cfu/$m^3$. Airborne fungi concentrations of 6 samples (12 %) exceeded 150 cfu/$m^3$, the guideline of WHO. The ratios of Indoor/Outdoor for airborne fungi ranged from 0.2 to 4.8 (mean = 1.6). Related factors were measured such as relative humidity, temperature, and laboratory area. Temperature and laboratory area showed no significant relations to concentrations of airborne fungi except for relative humidity in the laboratory Concentrations of fungi were significant different (p<0.01) between rainy or cloudy and sunny. However, there was no significant difference between general ventilation and nongeneral ventilation.

A 10b 100MS/s 27.2mW $0.8mm^2$ 0.18um CMOS Pipeline ADC with Various Circuit Sharing Schemes (다양한 회로 공유기법을 사용하는 10비트 100MS/s 27.2mW $0.8mm^2$ 0.18um CMOS Pipeline ADC)

  • Yoon, Kun-Yong;Lee, Se-Won;Choi, Min-Ho;Lee, Seung-Hoon
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.4
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    • pp.53-63
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    • 2009
  • This work proposes a 10b 100MS/s 27.2mW $0.8mm^2$ 0.18um CMOS ADC for WLAN such as an IEEE 802.11n standard. The proposed ADC employs a three-stage pipeline architecture and minimizes power consumption and chip area by sharing as many circuits as possible. Two multiplying DACs share a single amplifier without MOS switches connected in series while the shared amplifier does not show a conventional memory effect. All three flash ADCs use only one resistor ladder while the second and third flash ADCs share all pre-amps to further reduce power consumption and chip area. The interpolation circuit employed in the flash ADCs halves the required number of pre-amps and an input-output isolated dynamic latch reduces the increased kickback noise caused by the pre-amp sharing. The prototype ADC implemented in a 0.18um n-well 1P6M CMOS process shows the DNL and INL within 0.83LSB and 1.52LSB at 10b, respectively. The ADC measures an SNDR of 52.1dB and an SFDR of 67.6dB at a sampling rate of 100MS/s. The ADC with an active die area of $0.8mm^2$ consumes 27.2mW at 1.8V and 100MS/s.

The Effect of Co-existing Artemia sp. on the Rotifer Brachionus rotundigformis Population Growth (Rotife 배양조에 혼재된 Artemia가 Rotifer 의 증식에 미치는 영향)

  • Jung, Min-Min;Rho, Sum;Kim, Pil-Yun
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.99-103
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    • 1998
  • Artemia often observed as a co-existing organism in the mass culture tank of marine rotifer. The rotifer and Atremia are commonly used as food organisms in the marine fish larvae rearing. In this study, interspecific relation between the rotifer Brachionus rotundiformis (formely called S-type) and anostracan Artemia of the two developmental stages (0 and 19 day old after hatching) were investigated in the larboratory. The population growth of B. rotundiformis and one of the stage (nauplius or adult) of Artemia in mixed culture was compared with that of each single species culture. Culture period was 16 days. Every two days, the number of organisms in each species was counted and transferred to a fresh medium containing $7{\times}10^5$cells/ml of food Nannochloropsis oculata. Culture volume, temperature, salinity and photoperiod were set at 40ml, $25^{\circ}C.$, 22ppt and 24h all dark except to observation time, respectively. The rotifer population growth was greatly decreased by co-existence with Artemia. The coexisting Artemia suppressed the rotifer population growth due to it's high filtering speed for food (N. oculata). This study suggested that contamination by Artemia must be prevented for the stable rotifer production in the rotifer mass culture tank.

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