• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.4 no.2
• /
• pp.189-197
• /
• 1994

Analytic methods for Cr(VI) level in industrial hygienic field were suggested by the National Institute for Occupational Safety and Health(NIOSH method 7600, 7604). There were growing needs for measurement of Cr(III) and Cr(VI) levels simultaneously. Two analytical methods were suggested to determine Cr(III) and Cr(VI) levels simultaneously. The one is method by using reversed phase high peformance liquid chromatography(HPLC) and the other is by using ion exchange HPLC. The purpose of this work was to evaluate the usefulness of these two analytic methods. For the difference of ionic charges of Cr(III)-ethylendiamine tetraacetic acid(EDTA) chelate and $CrO_4{^-2}$, we could detect them simultaneously by ion exchange HPLC. Also, we attempted to determine the levels of Cr(III) and Cr(VI) chelated with sodium diethyldithiocarbamate(NaDDTC) by using reversed phase HPLC. The confirmation of Cr(III) and Cr(VI) were checked by fraction collector and nameless atomic absorption spectrometer. The optimal conditions for the formation of Cr(III)-EDTA chelate were two hours incubation period with pH 5. Cr(III)-EDTA and Cr(VI) in EDTA solution were successfully separated by anion exchange column using $Na_2CO_3/NaOH$ mixture as mobile phase. Peaks of Cr(III)-EDTA and Cr(VI) in EDTA were identified at 5 minutes and 7 minutes of retention time respectively by the ion exchange HPLC. The formation of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were twelve hours incubation period. Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were separated by reversed phase column using methanol and water mixture as mobile phase. Peaks of Cr(VI)NaDDTC and Cr(III)-NaDDTC chelates were identified at 13 minutes and 26 minutes of retention time respectively by the reversed phase HPLC. Due to reduction of Cr(VI) to Cr(III), it seems to be not suitable for simultaneous determination of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates by reversed phase HPLS. Simultaneos determination of Cr(III) and Cr(VI) by ion exchange HPLC was more accurate and simple method.

• The Korea Journal of Herbology
• /
• v.26 no.1
• /
• pp.41-46
• /
• 2011

Objectives : To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated. Methods : A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin $(PE)E_2$ were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats. Results : The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on $PGE_2$ production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.

• Weed & Turfgrass Science
• /
• v.2 no.3
• /
• pp.236-241
• /
• 2013

Introduction of rye living mulch in soybean production is important for labor-saving and reduction of herbicide usage for weeding by weed suppression and for soil conservation by mulching of rye. Soybean and rye were row-planted and broadcasted simultaneously at different planting times (May 15, May 25, June 5) and row widths (35 and 70 cm) in 2010, different soybean cultivars (Daepoong, Singi) and planting times (May 15 and June 1) in 2011, respectively, and investigated weed occurrence, early growth and yield of soybean. Acalypha australis, which is known to herbicide-resistant weed, could be controlled ecologically by rye living mulch. Occurrence of Acalypha australis was controlled effectively also by mixture of alachlor and linulon at no mulch in 2011. Early growth amount of soybean plant per area was increased by narrow row width (35 cm) at rye living mulch, which was helpful in the control of weeds, but grain yield of soybean with narrow row was decreased compared to conventional row width (70 cm) at the planting in early June. It is concluded that soybean planting as row width 70 cm in early June is proper at simultaneous planting with rye.

• Molecules and Cells
• /
• v.27 no.3
• /
• pp.329-336
• /
• 2009

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

• Journal of Korean Society of Environmental Engineers
• /
• v.32 no.10
• /
• pp.949-956
• /
• 2010

A method for simultaneous removal of nitrogen and phosphate from sewage by elemental sulfur denitrification with membrane bioreactor was proposed, and capacity $10\;m^3$/day of pilot plant was operated for 350 days. This study was investigated to have the effect of denitrification rate and T-P removal with the addition of Alum in Sulfur denitrification Reactor (SDR). The addition of Alum and alkalinity ($NaHCO_3$) in the effluent of MBR was tried to remove simultaneous phosphate and nitrogen in SDR. Characteristics of total nitrogen (T-N) and total phosphate (T-P) removal was compared without and with the addition of Alum as a coagulant. T-N removal without and with the addition of Alum was 92.1% and 87.8%, respectively. And denitrification efficiency was 93.8% and 87.1%, respectively. T-P removal rate was increased to 75.6% in SDR by addition of Alum (2.6~4 mg/L as Al), but T-P removal rate was about 26.7% without the addition of Alum. Therefore, denitrification rate was 6.7% of reduction but T-P removal rate was increased by addition of Alum.

• The Korean Journal of Physiology and Pharmacology
• /
• v.5 no.3
• /
• pp.259-270
• /
• 2001

The present study was attempted to investigate the characteristics of epibatidine on secretion of catecholamines (CA) from the isolated perfused model of the rat adrenal gland, and to establish the mechanism of action. Epibatidine $(3{\times}10^{-8}\;M)$ injected into an adrenal vein produced a great inhibition in secretory response of CA from the perfused rat adrenal gland. However, upon the repeated injection of epibatidine $(3{\times}10^{-8}\;M)$ at 15 min-intervals, CA secretion was rapidly decreased after second injection of epibatidine. However, there was no statistical difference between CA secretory responses of both 1st and 2nd periods by the successive administration of epibatidine at 120 min-intervals. Tachyphylaxis to releasing effects of CA evoked by epibatidine was observed by the repeated administration. Therefore, in all subsequent experiments, epibatidine was not administered successively more than twice only 120 min-intervals. The epibatidine-induced CA secretion was markedly inhibited by the pretreatment with atropine, chlorisondamine, pirenzepine, nicardipine, TMB-8, and perfusion of $Ca^{2+}-free$ Krebs solution containing EGTA, while was not affected by diphenhydramine. Moreover, the CA secretion evoked by ACh for 1st period $(0{\sim}4\;min)$ was greatly potentiated by the simultaneous perfusion of epibatidine $(1.5{\times}10^{-8}\;M),$ but followed by time-dependently gradual reduction after 2nd period. The CA release evoked by high potassium $(5.6{\times}10^{-8}\;M),$ for 1st period $(0{\sim}4\;min)$ was also enhanced by the simultaneous perfusion of epibatidine, but those after 2nd period were not affected. Taken together, these experimental data suggest that epibatidine causes catecholamine secretion in a calcium dependent fashion from the perfused rat adrenal gland through activation of neuronal cholinergic (nicotinic and muscarinic) receptors located in adrenomedullary chromaffin cells. It also seems that epibatidine-evoked catecholamine release is not relevant to stimulation of histaminergic receptors.

• Korean Journal of Soil Science and Fertilizer
• /
• v.39 no.5
• /
• pp.298-302
• /
• 2006

This study was carried out to establish low-input fertilization and seeding technique using the simultaneous with seeding and fertilizer application machine and band spotty applicator which were manufactured for experiment during cultivation of mulching for peanut(Arachis hypogaea L.). The labor hour for seeding by simultaneous with seeding and fertilizing machine was appeared over 90% reduction effect compared with control plot($17.3hr\;10a^{-1}$). In band spotty fertilization plots, the emergence date was delayed about 4 days and the seedling stand rate was decreased 11~18% compared with control plot(man power). The content of total nitrogen of soil after experiment was increased while the contents of organic matter, available phosphate and exchangeable potassium were decreased than before experiment. The content of nitrogen forming nitrate was increased in band spotty fertilization(BSF) plots by increasing the amount of applied fertilizer from early growth stage till the middle growth stage. Growth rate was increased in band spotty fertilization plots and the absorbed amount of phosphate and potassium for peanut were increased in 70% band spotty fertilization plot compared with control plot. Yield of peanut was increased 70% in band spotty fertilization plot due to high pod kernel ratio and ripened pod rate compared with control plot($3,150kg\;ha^{-1}$). It was found that 70% band spotty fertilization was more effective as fertilization method to reduce both environmental pollution and chemical nitrogen fertilizer in plastic film mulching cultivation of peanut.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.5
• /
• pp.815-820
• /
• 2004

The effect of capsaicin (0.02%) on the concentrations of liver and serum lipids, GOT and GPT activities, and hepatocyte morphology in male Sprague Dawley rats fed diets with or without erotic acid (1.0%) for 28 days was studied. Liver triglyceride concentration was increased by approximately 4.7-fold after erotic acid-feeding, wherease simultaneous feeding of capsaicin and erotic acid reduced its concentration by 38%. Orotic acidfeeding elevated liver cholesterol concentration, but an addition of capsaicin to the erotic acid diet resulted in the significant decrease in liver cholesterol. Capsaicin alone did not affect the liver triglyceride and cholesterol concentrations. Reduction of the serum concentrations of triglyceride and cholesterol by capsaicin was also observed in rats fed diets with erotic acid. The serum GOT and GPT activities were not significantly different among the experimental groups. Liver tissue morphology showed that the hepatocytes of erotic acid-feeding rats were a typical fatty liver with numerous fat droplets, whereas simultaneous feeding of capsaicin with erotic acid reduced the size and numbers of fat droplets. The present study demonstrated that the diet containing 0.02% capsaicin can prevent the erotic acid-induced fatty liver.

• Journal of Soil and Groundwater Environment
• /
• v.13 no.3
• /
• pp.52-58
• /
• 2008

Mn-impregnated activated carbon (Mn-AC) prepared at different conditions was applied in the treatment of synthetic wastewater containing both organic and inorganic contaminants. Phenol and As(III) was used as the representative organic and inorganic contaminants, respectively. After evaluation of the physicochemical characteristic and stability of Mn-AC, oxidation of As(III) as well as adsorption of phenol by activated carbon(AC) and Mn-AC were investigated in a batch reactor. To investigate the stability of Mn-AC, dissolution of Mn from each Mn-AC was measured pH ranging from 2 to 4. Although Mn-AC was unstable at a strong acidic condition, the dissoluted Mn was below 3 ppm at pH 4. XRD analysis of Mn-AC indicated that the mineral type of the impregnated manganese was $Mn_2O_3$. From the simultaneous treatment of As(III) and phenol by AC and Mn-AC, As(III) oxidation by Mn-AC was greater than that by AC at lower pH, while the reverse order was observed at higher pH. After impregnation of Mn onto AC, 13% decrease of the surface area was observed, causing 8% reduction of phenol removal. Considering removal properties of As(III) and phenol, Mn-AC could be applied in the simultaneous treatment of wastewater contaminated with multi-contaminants.

• Korean Journal of Soil Science and Fertilizer
• /
• v.40 no.6
• /
• pp.465-470
• /
• 2007

To evaluate the effect of companion plant (Phlox subulata and Glechoma hederacea var. longituba) on conservation of soil in slopped highland, coverage of crops and soil erosion were investigated with 3 different conditions. The coverage rate of Phlox subulata with Chinese cabbage cultivation was kept on approximately 100% from Chinese cabbage-transplanting to -harvest. The coverage rate of Glechoma hederacea var. longituba with Chinese cabbage cultivation was low at it's early stage, however, reached to approximately 100% at the time of Chinese cabbage harvest. In contrast, the coverage rate of Chinese cabbage cultivation without cover crop, and simultaneous transplanting with Chinese cabbage and cover crop were approximately 60%. Losses of soil and nitrate nitrogen were much lower in Phlox subulata with Chinese cabbage cultivation ($0.1{\sim}0.2ton\;ha^{-1}$, $0.2{\sim}0.4kg\;NO_3{^-}-N\;ha^{-1}$) than those in Chinese cabbage cultivation without cover crop ($20.8ton\;ha^{-1}$, $2.1kg\;NO_3{^-}-N\;ha^{-1}$), and simultaneous transplanting with Chinese cabbage and cover crop ($8.9{\sim}10.5ton\;ha^{-1}$, $1.5{\sim}2.2kg\;NO_3{^-}-N\;ha^{-1}$). Cover plants suppressed the weed occurrence up to about 70%. Live mulching with cover plants set a good effects on weed suppression and reduction of soil and nutrient loss. Therefore intercropping with Phlox subulata will make great contributions to soil conservation in Chinese cabbage cultivated highland.