• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.667-672
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• 2019

We compared two integration systems for stable expression of heterologous genes in Saccharomyces cerevisiae. A Candida glabrata-derived gene was used as the selective marker for the Cre/loxP system, and XYLP, XYLB, GRE3, and XYL2 genes were used as model heterologous genes and ligated into the universal pRS-CMT vector. The resulting pRS-XylP, pRS-XylB, pRS-Gre3, and pRS-Xyl2 plasmids were sequentially integrated into yeast chromosome VII by four integration processes (marker rescue and gene integration). The four introduced genes were successfully expressed. Further, the pRS-PBG2 plasmid harboring expression cassettes for the four genes was constructed for one-step integration. The four genes that were introduced were stably maintained as a gene cluster and were simultaneously expressed. The one-step integration was more effective for the simultaneous integration and expression of the four genes related to xylan/xylose metabolism. This method will enable the generation of a useful biosystem through appropriate use of gene integration methods.

• Journal of Life Science
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• v.19 no.3
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• pp.322-326
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• 2009

Glutathione is a well-known chemotherapeutic agent and a popular nutritional supplement for liver disease and oxidative stress. Our previous studies reported the suppressive effects of the glutathione-enriched Saccharomyces cerevisiae FF-8 (FF-8) strain on carbon tetrachloride- and alcohol-induced oxidative stress in rats. The purpose of the current study was to investigate the effect of the FF-8 strain on lipid peroxidation in tissues of rats with orotic acid (OA)-induced fatty liver. OA treatment showed a significant decrease in body weight gain compared to the normal diet, and simultaneous addition of FF-8 and OA had the same effect. OA treatment produced an increase in liver weight, however, this also increased with simultaneous addition of FF-8 and OA. Liver lipid peroxidation was significantly increased by OA, but was significantly decreased by FF-8 strain treatment. This same tendency was found in the kidney and heart. Concentration levels of hepatic glutathione and zinc are known to be closely associated with the antioxidant system, and OA treatment led to reductions in liver glutathione and zinc concentrations, whereas these were significantly increased by FF-8 strain treatment in OA feeding rats. These results suggest that the glutathione-enriched S. cerevisiae FF-8 strain may positively mediate orotic acid-induced oxidative stress by enhancing glutathione and zinc levels in rat livers.

• Journal of The Korean Association For Science Education
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• v.2 no.1
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• pp.31-51
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• 1980

In order to research into the devel of the concept of movement and speed in our own way and compare with the Piaget's method, and then find out the formative period of such development, we have put the 192 primary school children as the model and obtained the following results. 1. As their grade upyards, all the experimental subjects showed the gradual progress generally regardless of sex or regions, while only the relative speed showed irregular progress. 2. There was no experimental subject which showed any remarkable sexual characteristics. But in the relative movement, the lower grade boys and higher grade girls made progress. In the subjects of circulation movement, intuition of speed, relative speed and speed of simultaneous movement, the boys marked better progress, whilst the girls were advanced a little in the speed of continuous movement with, different distance and time. 3. There was no conspicuous difference between the urban and rural areas, except a slight tendency that the urban children made better progress in the change of progressing direction, native continuous procedure of periodical movement, travelling road, and the speed of continuous movement with different distance and time whereas the rural children were more advanced in the relative movement, relative speed and intuition of speed. But it should be 'taken into consideration that the rural regions in our case were relatively developed in comparison with the traditional rural communities, which may explain about little regional difference. 4. Comparing our research results with the Piaget's theory, we have reached below results. Our research reports that the formative period of the conception of the problems of diverse progressing direction and a native continuous procedure of periodical movement was simultaneous, at the Piagetian level(at the fourth grade) which coincides with Piagetian theory. The travelling road should be made up a little lower at 2A/2B according' to Piagetian Level, whereas it was formed at the 4th grade as shown in our previous research. Intuition of speed should belong to the prior stage to concrete operation, but our research shows it was formed late at the 4th grade. Composition of displacement was made at the 6th grade, and it was almost equal to the first stage of formal operation(3A). But in the subjects of relative movement relative speed, the speed of continuous movement with different distance and time, relation and preservation of invariable speed, and accelerated motion, even 6th grade children marked a poor record. Summed up, the procedure conception as a basic movement conception coincides with the Piagetion level. But as for speed intuition, relative speed and speed fixation, it was Jar behind Piagetian level. Therefore it is required that we have to concentrate on the systematic training in these parts on the spot.

• Journal of the Korean Data and Information Science Society
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• v.22 no.4
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• pp.679-690
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• 2011

Two widely used approaches for improving the quality of the output of a process are statistical process control (SPC) and automatic process control (APC). In recent hybrid processes that combine aspects of the process and parts industries, process variations due to both the inherent wandering and special causes occur commonly, and thus simultaneous application of APC and SPC schemes is needed to effectively keep such processes close to target. The simultaneous implementation of APC and SPC schemes is called integrated process control (IPC). In the IPC procedure, the output variables are monitored during the process where adjustments are repeatedly done by its controller. For monitoring the APC-controlled process, control charts can be generally applied to the output variable. However, as an alternative, some authors suggested that monitoring the input variable may improve the chance of detection. In this paper, we evaluate the performance of several monitoring statistics, such as the output variable, the input variable, and the difference variable, for efficiently monitoring the APC-controlled process when we assume IMA(1,1) noise model with a minimum mean squared error adjustment policy.

• Journal of the Korean Geographical Society
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• v.36 no.5
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• pp.561-577
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• 2001

Urban spatial structure is closely related to the spatial distribution of urban economic activities. The spatial distribution pattern is no more than an aggregated expression of the location and/or relocation behavior of individual firms and establishments. In this respect, it is important to identify and examine the factors that affect the spatial behavior of individual firms for a more comprehensive understanding of urban space. Agglomeration economies are one of the most prominent urban economic phenomena in the modern metropolitan area. Most firms in an urban space seek external economies through the spatial clustering of their activities. Agglomeration economies feature prominently in the analysis of urban economic structure across urban areas. While the agglomeration economies between cities focus at the macro-scale of analysis, such economies within any given city focus more on the micro geographical scale. There have been a number of researches on agglomeration economies, among which there are relatively few approaches based on an intra-urban context. This proper explores the agglomeration economies at the micro scale and tries to reseal the spatial realization of the agglomeration economies within and between sectors. Three sectors are considered in the analysis; manufacturing, retail and service. The model is based on simultaneous equation systems combined with spatially weighted variables and estimated by the KRP estimators.

• The Korean Journal of Physiology and Pharmacology
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• v.5 no.3
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• pp.259-270
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• 2001

The present study was attempted to investigate the characteristics of epibatidine on secretion of catecholamines (CA) from the isolated perfused model of the rat adrenal gland, and to establish the mechanism of action. Epibatidine $(3{\times}10^{-8}\;M)$ injected into an adrenal vein produced a great inhibition in secretory response of CA from the perfused rat adrenal gland. However, upon the repeated injection of epibatidine $(3{\times}10^{-8}\;M)$ at 15 min-intervals, CA secretion was rapidly decreased after second injection of epibatidine. However, there was no statistical difference between CA secretory responses of both 1st and 2nd periods by the successive administration of epibatidine at 120 min-intervals. Tachyphylaxis to releasing effects of CA evoked by epibatidine was observed by the repeated administration. Therefore, in all subsequent experiments, epibatidine was not administered successively more than twice only 120 min-intervals. The epibatidine-induced CA secretion was markedly inhibited by the pretreatment with atropine, chlorisondamine, pirenzepine, nicardipine, TMB-8, and perfusion of $Ca^{2+}-free$ Krebs solution containing EGTA, while was not affected by diphenhydramine. Moreover, the CA secretion evoked by ACh for 1st period $(0{\sim}4\;min)$ was greatly potentiated by the simultaneous perfusion of epibatidine $(1.5{\times}10^{-8}\;M),$ but followed by time-dependently gradual reduction after 2nd period. The CA release evoked by high potassium $(5.6{\times}10^{-8}\;M),$ for 1st period $(0{\sim}4\;min)$ was also enhanced by the simultaneous perfusion of epibatidine, but those after 2nd period were not affected. Taken together, these experimental data suggest that epibatidine causes catecholamine secretion in a calcium dependent fashion from the perfused rat adrenal gland through activation of neuronal cholinergic (nicotinic and muscarinic) receptors located in adrenomedullary chromaffin cells. It also seems that epibatidine-evoked catecholamine release is not relevant to stimulation of histaminergic receptors.

• Journal of the Korean Geotechnical Society
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• v.26 no.10
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• pp.61-68
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• 2010

A pilot-scale model test was performed to estimate the availability of new material, Fe-loaded zeolite, as the filling material in permeable reactive barrier (PRB) against the contaminated groundwater with both Cd and Cr(VI). Aquifer was simulated by filling up a large scale soil tank with sands, and mobilizing the water flow by the head difference of water level in both ends of the tank. Then, the mixture of concentrated Cd and Cr(VI) solution was injected into the aquifer to form a contaminant plume, and its behavior through Fe-loaded zeolite barrier was monitored. The test results showed that Fe-loaded zeolite barrier successfully treated the contaminant plume containing both Cd and Cr(VI) and that the immobilized contaminants in the barrier were not desorbed or released. The results indicated that the Fe-loaded zeolite could be a promising material in PRBs against the multiple contaminants with different ionic forms like Cr(VI) and Cd.

• Korean Journal of Social Welfare Studies
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• v.41 no.4
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• pp.81-105
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• 2010

This study is aimed to investigate the effects of stress on suicidal impulse, considering the mediating effects of existent spirituality, family support, and depression. The data, collected from 1,000 adults were examined by the statistics software SPSS 17.0 and AMOS 17.0, in which descriptive statistics, structural equation model analysis, and multi-group simultaneous analysis are utilized. The study shows that, from the structural equation modeling, the stress has positive effects on depression and suicidal impulses, but negative effects on existent spirituality. Existent spirituality acts as a protective factor, negatively affecting the suicidal impulse. Depression has positive effects on suicidal impulse. Therefore, existent spirituality and depression have mediational effects on the relationship between stress and suicidal impulse. The results of multi-group simultaneous analysis imply that there are no age and sex differences. In conclusion, social workers need to make great efforts to exterminate stress, and treat depression at the first priority, because the depression is a major sign of suicide. As a protective factor, strengthening existent spirituality is a very effective way to prevent a suicidal impulse.

• Journal of Korean Society of Transportation
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• v.24 no.3 s.89
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• pp.17-27
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• 2006

Car following model is a theory that examines changes of condition and interrelationship of acceleration deceleration. headway, velocity and so on closely based on the hypothesis that the Posterior vehicle always follows the preceding vehicle. Car following mode) which is one of the research fields of microscopic traffic flow was first introduced in 1950s and was in active progress in 1960s. However, due to the limitation of data gathering the research depression was prominent for quite a while and then soon was able to tune back on track with development in global positioning system using satellite and generalization of computer use. Recently, there has been many research studies using reception materials of global Positioning system(GPS). Introducing GPS technology to traffic has made real time tracking of a vehicle position possible. Position information is sequential in terms of time and simultaneous measurement of several vehicles in continuous driving is also practicable. Above research was focused on judging whether it is feasible to overcome the following model research by adopting the GPS reception device that was restrictively proceeded due to the limitation of data gathering. For practical judgment, we measured the accuracy and confidence level of the GPS reception devices material by carrying out a practical experiment. Car following model is also being applied in simulations of traffic flow analysis, but due to the difficulty of estimating parameters the basis of the above result. it is our goal to produce an accurate calibration of car following model's parameters that is suitable in this domestic actuality.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.24 no.4
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• pp.295-304
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• 2012

This study proposed a two-dimensional horizontal numerical model based on the nonlinear shallow water wave equations to simulate tsunami propagation and coastal inundation. We numerically investigated the possible impacts of tsunami caused by the triple interlocked Tokai, Tonankai and Nankai Earthquakes on the Jeju coastal areas, using the proposed model. The simultaneous Tokai, Tonankai and Nankai Earthquakes were created a virtual tsunami model of an M9.0 earthquake. In numerical analysis, a grid nesting method for the local grid refinement in shallow coastal regions was employed to sufficiently reproduce the shoaling effects. The numerical model was carefully validated through comparisons with the data collected during the tsunami events by 2011 East Japan Earthquake and 1983 central East Sea Earthquake (Nihonkai Chubu Earthquake). Tsunami propagation triggered by the combined Tokai, Tonanakai and Nankai, Earthquakes was simulated for 10 hours to sufficiently consider the effects of tsunami in the coastal areas of Jeju Island. The numerical results revealed that water level fluctuation in tsunami propagation is greatly influenced by water-depth change, refraction, diffraction and reflection. In addition, the maximum tsunami height numerically estimated in the coastal areas of Jeju Island was about 1.6 m at Sagye port.