• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.285-291
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• 2004

A simulated moving bed (SMB) chromatography system is a powerful tool for preparative scale separation, which can be applied to the separation of chiral compound. We have de-signed our own lab-scale SMB chromatography using 5 HPLC pumps, 6 stainless steel columns and 4 multi-position valves, to separate a racemic mixture of ketoprofen in to its enantiomers. Our design has the characteristics of the low cost for assembly for the SMB chromatography and easy repair of the unit, which differs from the designs suggested by other investigators. It is possible for the flow path through each column to be independently changed by computer control, using 4 multi-position rotary valves and 5 HPLC solvent delivery pumps. In order to prove the operability of our SMB system, attempts were made to separate the (S)-ketoprofen enantiomer from a ketoprofen racemic mixture. The operating parameters of the SMB chromatography were calculated for ketoprofen separation from a batch chromatography experiment as well as by the triangle theory. With a feed concentration of 1 mg/mL, (S)-ketoprofen was obtained with a purity of 96% under the calculated operating conditions.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.549-553
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• 2003

SMB(simulated moving bed) chromatography system has been developed to realize continuous separation and save solvent consumption for binary mixture such as chiral compounds in especial. The parameters of SMB chromatography system can be calculated from mass balance equations of true moving bed chromatography, and they are used in design of 6-column SMB chromatography. We can separate 1'R-25 and 1'S-2S enantiomers as a raffinate product in 95% of purity using assembled SMB chromatography system.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.506-510
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• 2003

SMB(simulated moving bed) chromatography system has been developed to realize continuous separation and save solvent consumption for binary mixture in especial. The parameters of SMB chromatography system can be calculated from mass balance equations of true moving bed chromatography, and they are used in design of 6-column SMB chromatography. We can separate S-ketoprofen enantiomer as a raffinate product in 85% of purity and 0.3mg/ml using assembled SMB chromatography system.

• KSBB Journal
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• v.15 no.6
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• pp.541-547
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• 2000

Simulated moving bed (SMB) chromatography has been investigated in order to separate chiral compounds for pharmaceutical use. SMB utilizes the principle of true moving bed (TMB) chromatography, and has the advantages of low solvent usage, flexible configuration of columns and hardwares, and high productivity of the chiral compounds over the TMB. Large scale separation of xylene isomers and saccharides has been conducted since 1960s. However, the application of SMB in the fine chemical industries is still in the infant stage. The study of SMB for the chiral compounds production was initiated in the mid 1990s and further researches are actively undergoing. This review summarizes the principle of SMB as well as the chemistry of chiral stationary phase and chiral compounds.

• Korean Journal of Hydrosciences
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• v.5
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• pp.1-16
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• 1994

In this paper distributed models for simulating spatially and temporally varied moving storm in a watershed were developed. The complete simulation in a watershed is achieved through two sequential flow simulations which are overland flow simulation and channel network flow simulation. Two dimensional continuity equation and momentum equation of kinematic approximation were used in the overland flow simulation. On the other hand, in the channel network simulation two types of governing equations which are one dimensional continuity and momentum equations between two adjacent sections in a channel, and continuity and energy equations at a channel junction were applied. The finite difference formulations were used in the channel network model. Macks Creek Experimental Watershed in Idaho, USA was selected as a target watershed and the moving storm on August 23, 1965, which continued from 3:30 P.M. to 5:30 P.M., was utilized. The rainfall intensity fo the moving storm in the watershed was temporally varied and the storm was continuously moved from one place to the other place in a watershed. Furthermore, runoff parameters, which are soil types, vegetation coverages, overland plane slopes, channel bed slopes and so on, are spatially varied. The good agreement between the hydrograph simulated using distributed models and the hydrograph observed by ARS are Shown. Also, the conservations of mass between upstreams and downstreams at channel junctions are well indicated and the wpatial and temporal vaiability in a watershed is well simulated using suggested distributed models.

• Water for future
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• v.26 no.1
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• pp.81-91
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• 1993

In this paper, a moving storm in the real watershed was simulated using a distributed model. Macks Creek Experimental Watershed in Idaho, USA was selected as a target watershed and the moving storm of August 23, 1965, which continued from 3:30 P.M. to 5:30 P.M., was utilized. The rainfall intensity of the moving storm in the watershed was temporally varied and the storm was continuously moved from one place to the other place in a watershed. Furthermore, runoff parameters, which are soil types, vegetative cover percentages, overland plane slopes, channel bed slopes and so on, are spatially varied. The model developed in the previous paper was utilized as a distributed model for simulating the moving storm. In the model, runoff in a watershed was simulated as two parts which are overland flow and channel flow parts. The good agreement was obtained between a simulated hydrograph using a distributed model and an observed hydrograph. Also, the conservations of mass are well indicated between upstream and downstream at channel junctions.

• Korean Chemical Engineering Research
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• v.46 no.4
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• pp.732-738
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• 2008

Simulated moving bed (SMB) process is a continuous chromatographic technology used to separate a large amount of petrochemicals, fine chemicals, pharmaceuticals, and so on, drawing a great attraction of related industries. With the recent development of biotechnology, the SMB process has been adopted for the separation of various useful bio-products. Attempts to understand the separation mechanism of the SMB process in many aspects are reported in many publications. These researches have dealt with the improvement of SMB for easier operation and solving problem in process. The feed mixture fed into the SMB process may be of different concentration batch by batch rather than in uniform concentration. Retention behaviors of feed (psicose (A) and fructose (B) mixture) existing in the SMB unit in dynamic steady-state and feed (psicose (C) and fructose (D) mixture) newly injected into the SMB were analyzed. It was observed that the existing components, (A) and (B), were eluted relatively faster as the injection time of new feed was earlier during the port-switching period. In addition, the components (C) and (D) were eluted earlier as fresh feed was injected earlier in a port-switching time.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.81-88
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• 2016

Limonene is orange flavored natural material that is mainly contained in mandarine and lemon peels. D-limonene was extracted from cold-storaged mandarine peel by using Soxhlet extractor at $120^{\circ}C$ for 2 hours with ethanol as solvent. Henry constants of d-limonene and impurity were calculated as $H_{Lim}=8.55$ and $H_{imp}=0.223$ from the result of HPLC analysis. 4-bed SMB of limonene simulation with $0.46{\times}25cm$ columns was conducted by using Aspen chromatography program. Then effective condition for purity was found by changing $m_2$ and $m_3$ values in triangle diagram. The highest purity was 98.59% at $m_2=2.57$, $m_3=9.55$. For this case, feed, desorbent, extract, and raffinate flow rates were 1 mL/min, 1.19 mL/min, 0.857 mL/min and 1.34 mL/min, respectively. Scale-up simulation was also conducted by increasing column diameter from 0.46 cm to 1.6 cm for getting the same efficiency. The increased flow rates were 12 mL/min, 14 mL/min, 10 mL/min, and 16 mL/min for feed, desorbent, extract, and raffinate. It was possible to scale-up with maintaining same limonene purity because linear isotherms of limonene and impurity were assumed.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.527-527
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• 2005

• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.35-39
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• 2006

This study was performed to understand temperature effect on retention behavior of fructose and glucose as sugars and lactic acid and acetic acid as organic acids on poly (4-vinylpyridine) resin. The pulse tests were performed to understand temperature effect on retention time of sugars and the results were not shown large change. As it was able to predict with PVP resin not to be used for sugar separation generally, the results were shown poor resolution for separation of sugars and temperature effect on the resolution change of sugars also was not large. On the other hand, in the case of organic acids on PVP resin, the pulse tests were shown temperature effect on the retention behavior was very large. So, the frontal analyses were performed to understand quantitative adsorption behavior of organic acids at 35 and $65^{\circ}C$. These adsorption characteristics of organic acids with PVP resin system can be used to preparative chromatographic process such as SMB (simulated moving bed).