• 생명과학회지
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• 제16권2호
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• pp.219-224
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• 2006

ISSR markers를 이용하여 수박 18품종의 유전적 유연관계를 분석하여 얻어진 결과를 요약하면 다음과 같다. 수박 18품종의 genomic DNA와 ISSR primer 100개를 PCR 반응시킨 결과 다형성을 뚜렷하게 나타내는 primer는 21개 이였으며, 이들 primer에 의해 증폭된 밴드는 105개 이였고 다형성을 보이는 밴드는 58개 였으며 증폭된 DNA 단편의 크기는 $0.2{\sim}5.0kb$ 사이에 위치하였다. 다형성을 나타낸 primer는 18개의 anchored primer와 3개의 non-anchored primer로 구분되었고 모든 anchored primer는 2개의 염기서열이 반복된 형태를 나타내었으며, non-anchored primer보다. 다형성 정도가 높게 나타났다. 수박 18품종은 유전적 유사도 값 0.42를 기준으로 할 때 18개 품종을 2개의 그룹 으로 구분할 수 있었으며, 국내에서 육성된 품종은 유전적 유사도가 아주 높은 것으로 분석 되었고, 이들 품종은 수박의 과형에 따라 유사하게 구분되었다.

• Journal of Forest and Environmental Science
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• 제24권2호
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• pp.61-68
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• 2008

Acrylamide is present as a contaminant in heated food products, predominantly from the precursor asparagine. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. In order to assess the feasibility of microsatellite primers as markers for DNA damage, the study was conducted on common bean (Phaseolus vulgaris L.) exposed to different concentrations of acrylamide. Polymorphisms were abundant among plant samples treated with acrylamide in comparison to control (untreated one) tested with 4- tri-nucleotide, 2 tetra-nucleotide, and 3- dinucelotide primers. The primer (CCG)4 was the best tested primer to generate polymorphism between the DNA of plants treated or not by acrylamide. Polymorphisms became evident as the presence and absence of DNA fragments in treated samples compared with the untreated one. The highest number of DNA variation on ISSR patterns was observed at the micromollar concentrations of acrylamide. Acrylamide was able to induce DNA damage in non concentration-dependent manner with effectiveness at micromollar concentrations. This study demonstrated that ISSR markers can be highly reliable for identification of DNA damage induced by acrylamide.

• 식물분류학회지
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• 제52권3호
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• pp.156-172
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• 2022

Campsis grandiflora (Thunb.) K. Schum is an ornamental species with various useful biological effects. The chloroplast genome of C. grandiflora isolated in Korea is 154,293 bp long (GC ratio: 38.1%) and has four subregions: 84,121 bp of large single-copy (36.2%) and 18,521 bp of small single-copy (30.0%) regions are separated by 24,332 bp of inverted repeat (42.9%) regions including 132 genes (87 protein-coding genes, eight rRNAs, and 37 tRNAs). One single-nucleotide polymorphism and five insertion and deletion (INDEL) regions (40-bp in total) were identified, indicating a low level of intraspecific variation in the chloroplast genome. All five INDEL regions were linked to the repetitive sequences. Seventy-two normal simple sequence repeats (SSRs) and 47 extended SSRs were identified to develop molecular markers. The phylogenetic trees of 29 representative Bignoniaceae chloroplast genomes indicate that the tribe-level phylogenic relationship is congruent with the findings of previous studies.

• Mycobiology
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• 제47권4호
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• pp.527-532
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• 2019

We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (M_AF) and the average number of alleles (N_A) were 0.50 and 5.47, respectively. The average number of genotypes (N_G), observed heterozygosity (H_O), expected heterozygosity (H_E), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that M_AF was negatively correlated with N_G (-0.683), N_A (-0.600), H_O (-0.584), and PIC (-0.941). N_G, N_A, H_O, and PIC were positively correlated with other polymorphic parameters except for M_AF. UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.

• The Plant Pathology Journal
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• 제36권1호
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• pp.11-28
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• 2020

Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes. The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible. The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.

• 한국작물학회지
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• 제46권4호
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• pp.334-340
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• 2001

Two different types of molecular markers, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP), were used to measure genetic diversity among five Korean, eight Thai, and three wild soybeans. For SSR analysis, a total of 20 markers were surveyed to detect polymorphisms. For SNP analysis, four primers were designed from consensus sequence regions on disease resistance protein homolog genes, and used to amplify the genomic region. The PCR products were sequenced. A number of polymorphic SSR and SNP bands were scored on all genotypes and their genetic similarity was measured. Clustering analysis was performed independently on both types of markers. Clustering based on SSR markers separated the genotypes into three main groups originated from Korea, Thailand, and wild soybeans. On the other hand, two main groups were classified using SNP analysis. It seemed that SSR was more informative than SNP in this study. This may be due to the fact that SNP was surveyed on the smaller genomic region than SSR. Grouping based on the combined data of both markers revealed similar results to that of SNP rather than that of SSR. This might be due to the fact that more loci from SNP were considered to measure genetic relatedness than those from the SSR.

• 한국버섯학회지
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• 제15권4호
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• pp.273-278
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• 2017

느티만가닥버섯은 맛과 기능성이 풍부한 버섯으로 많이 활용되고 있다. 하지만 긴 재배기간과 낮은 자실체 수확량, 균이 오랜시간 배양되어 오염이 쉽게 발생되는 문제점을 극복할 새로운 품종육성이 필요하다. 이에 따라 육종모본으로 활용되는 느티만가닥 55균주의 종내 유전자원의 정확한 정보를 얻고자 분자유전학적 ISSR 마커분석을 활용하였다. 사용된 마커 중에서 ISSR 13과 15 마커를 사용했을 때 다형성이 분석되었으며 특히 ISSR 15마커 분석으로 다형성이 쉽게 구분되었다. UPGMA분석법으로 계통도를 분석하였을 때, 일부 백색을 가지고 있는 KMC03106, KMC03107, KMC03108 3 균주가 두 마커 모두에서 가까운 유연관계를 가졌으며, ISSR 15는 수집년도에 따라 3개의 그룹으로 구분되는 것을 확인할 수 있었다. 이 결과로 ISSR마커의 다형석 분석은 느티만가닥버섯의 몇몇 균주에서는 갓색의 유연관계 확인과 수집 시기에 따른 유전변이 구분이 가능하며 자원의 유전적 다양성 확인할 수 있어, 느티만가닥버섯의 품종육성을 위한 효율적인 모본 선발이 가능 할 것으로 사료된다.

• 한국버섯학회지
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• 제15권3호
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• pp.139-144
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• 2017

양송이(Agaricus bisporus)는 국내에서 2015년 약 10,757톤이 생산되어 5번째로 많이 생산되는 버섯이다. 본 연구에서는 ISSR 마커를 사용하여 국내 수집균주와 상업품종간의 유전적 다양성을 분석하였다. 이를 위해 우선, 다양한 마커 중 양송이 속 내 비교 분석이 가능다고 알려진 ISSR마커를 선발하였다. 분석한 마커는 ISSR 807, 808, 809, 810, 811, 834, 835, 836, 841, 842, P3, P8, P17, P22, P30, P38 and P39 총 16종이였고 이들 중 육종에서 모본선발을 위한 효율적인 마커를 선발하기 위하여 양송이 수집균주 ASI 1110, 1114, 1115, 1238, 1246, 1365, 1366, 1369 등 8종을 선발하여 수행하였다. 그 결과 ISSR P31, P38, P39 마커에서 종내 구분이 가능한 다양한 밴드가 나타났다. 이를 바탕으로 선발된 3종의 마커를 이용하여 국내 수집균주와 새아, 새연 등의 상업품종이 포함된 39균주를 UPGMA 프로그램을 통하여 유연관계를 분석하였다. 국내 수집균주와 상업품종간의 계통도를 분석한 결과, 국내 수집균주와 상업품종들이 다른 그룹을 형성하는 것을 확인하였다. 이 결과를 근거로 ISSR P31, P38, P39가 상업품종을 구분할 수 있는 마커로 활용될 것이라 기대된다.

• 생명과학회지
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• 제16권6호
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• pp.1001-1005
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• 2006

SSR markers를 이용하여 벼의 품종간 유전적 유연관계 분석과 품종식별 방법에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. SSR primer 50개와 벼 보급종 21품종을 PCR 반응시킨 결과 다형성을 뚜렷하게 나타내는 primer는 23개였으며, 각 marker에 의해 발생된 대립유전자의 수는 $2{\sim}9$까지 검출되었고, 평균값은 3.00개로 나타났다. 유전적 다형성 정도를 나타내어 주는 SSR marker의 PIC 값은 최소 0.091에서부터 최대 0.839까지 다양하게 분석되었다. SSR marker를 이용하여 분석된 벼 21품종에 대한 전체 유전적 유사도는 $0.59{\sim}0.92$의 범위에 속하였고 유사도 지수 0.65를 기준으로 할 때 4개의 그룹으로 구분되었다. SSR marker중에서 RM206, RM225, RM418, RM478은 marker genotype에 의해 21 품종에 대해 각각 고유한 밴드 특성을 나타내어 품종판별이 가능한 것으로 나타났다. 금후 이 연구결과는 벼 보급종의 품종식별을 위해 효과적으로 이용될 수 있는 것으로 나타났다.

• 식물분류학회지
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• 제48권3호
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• pp.195-200
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• 2018

Genetic assessments of rare and endangered species are among the first steps necessary to establish the proper management of natural populations. Transcriptome-derived single-sequence repeat markers were developed for the Korean endangered species Astilboides tabularis (Saxifragaceae) to assess its genetic diversity. A total of 96 candidate microsatellite loci were isolated based on transcriptome data using Illumina pair end sequencing. Of these, 26 were polymorphic, with one to five alleles per locus in 60 individuals from three populations of A. tabularis. The observed and expected heterozygosity per locus ranged from 0.000 to 0.950 and from 0.000 to 0.741, respectively. These polymorphic transcriptome-derived simple sequence repeat markers would be invaluable for future studies of population genetics and for ecological conservation of the endangered species A. tabularis.