Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Isolation and characterization of EST-SSR markers for Astilboides tabularis (Saxifragaceae), endangered species in Korea

  • JUNG, Eui-Kwon (Department of Life Science, Hallym University) ;
  • KANG, Dae-Hyun (Multidisciplinary Genome Institute, Hallym University) ;
  • YOO, Ki-Oug (Department of Biological Sciences, Kangwon National University) ;
  • KWAK, Myounghai (Plant Resources Division, National Institute of Biological Resources) ;
  • KIM, Young-Dong (Department of Life Science, Hallym University) ;
  • KIM, Bo-Yun (Multidisciplinary Genome Institute, Hallym University)
  • Received : 2017.09.04
  • Accepted : 2018.09.28
  • Published : 2018.09.30
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Abstract

Genetic assessments of rare and endangered species are among the first steps necessary to establish the proper management of natural populations. Transcriptome-derived single-sequence repeat markers were developed for the Korean endangered species Astilboides tabularis (Saxifragaceae) to assess its genetic diversity. A total of 96 candidate microsatellite loci were isolated based on transcriptome data using Illumina pair end sequencing. Of these, 26 were polymorphic, with one to five alleles per locus in 60 individuals from three populations of A. tabularis. The observed and expected heterozygosity per locus ranged from 0.000 to 0.950 and from 0.000 to 0.741, respectively. These polymorphic transcriptome-derived simple sequence repeat markers would be invaluable for future studies of population genetics and for ecological conservation of the endangered species A. tabularis.

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References

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