• 수산해양기술연구
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• 제56권2호
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• pp.94-104
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• 2020

An ecological study for species composition and abundance of the fish assemblages in the coastal waters of Gujora and Mangchi of Geoje, Korea was conducted using monthly larval collection by an RN80 net from June, 2018 to May, 2019. A total of the 5 Orders, 15 Families, 21 species were collected. The dominant species were Ammodytes japonicus and Engraulis japonicas. Gobiidae was the most dominant species in June, Spratelloides gracilis in July, Chelidonichthys sp. in August, Gobiidae in September, Repomucenus beniteguri in October, Sebastiscus marmoratus in November, Sebastiscus marmoratus and Hexagrammos otakii in December of 2018, Pleuronectes yokohamae in January, Ammodytes japonicus in Febraury, Engraulis japonicus in March, Sebastes sp. and Gobiidae in April and Engraulis japonicus in May of 2019. The highest number of species and individuals were found in July and the smallest in August. Thus, the diversity index was the highest in July and the lowest in August. As a result of the similarity by month was divided into Group I in January and February and Group II in April, June and September. The most dominant species in the Group I were the Ammodytes japonicus and Sebastes inermis, and the most dominant species in the Group II was Gobiidae.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1605-1614
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• 2022

The strains associated with foodborne Salmonella enterica Thompson outbreaks in Korea have not been identified. Therefore, we characterized S. Thompson strains isolated from chocolate cakes linked to foodborne outbreaks in Korea. A total of 56 strains were isolated from preserved cake products, products in the supply chain distribution, the manufacturer's apparatus, and egg white liquid products used for cream preparation. Subsequently, serological typing, pathogenic gene-targeted polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), and whole-genome multi-locus sequence typing (wgMLST) were performed to characterize these isolates. The antigen formula of all isolates was 7:k:1,5, namely Salmonella enterica subsp. enterica Serovar Thompson. All 56 isolates harbored invA, his, hin, and stn, and were negative for sefA and spvC based on gene-targeted PCR analyses. Based on PFGE results, these isolates were classified into one group based on the same SP6X01.011 pattern with 100% similarity. We selected 19 strains based on the region and sample type, which were subjected to wgMLST. Although the examined strains showed 100% similarity, they were classified into seven clusters based on allelic differences. According to our findings, the cause of these outbreaks was chocolate cake manufactured with egg white liquid contaminated with the same Salmonella Thompson. Additionally, comparative analysis of wgMLST on domestic isolates of S. Thompson from the three outbreaks showed genetic similarities of over 99.6%. Based on the results, the PFGE and wgMLST combination can provide highly resolved phylogeny and reliable evidence during Salmonella outbreak investigations.

• 한국균학회지
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• 제25권3호통권82호
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• pp.176-190
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• 1997

Isozyme PAGE에 의한 isozyme polymorphism 및 random amplified polymorphic DNA(RAPD) pattern의 비교를 통하여 한국에서 수집된 63개의 표고 버섯(Lentinus edodes)품종의 유연관계를 조사하여 보았다. NTSYS PC program을 통하여 비교에 사용되어진 3가지 isozyme 즉 esterase, peroxidase, acid phophatase 등은 각각 10, 7, 3개의 isozyme polymorphism type으로 나뉘어질 수 있었고, 이러한 3개의 isozyme type들은 group-average method를 이용하여 최종적으로 6개의 집단으로 구분되어질 수 있었다. 또한 RAPD를 통한 표고 품종간 유연관계 비교는 사용되어진 총 20개의 random primer 들중 3개의 primer(OPA 03, OPA 18, OPA 19)가 품종간 polymorphism을 보였고 사용되어진 전체 random primer의 PCR product들의 집단 분석을 통해 최종적으로 5개의 집단으로 구분할 수 있었다. 사용되어진 두 가지 방법 즉, isozyme polymorphism및 RAPD pattern에 의해 구분되어진 63개 표고 버섯 품종의 subgroup level에서의 절대적인 유사성은 관찰할 수 없었다. Yii-Mattila(1996) 등에 의하면 품종간 비교시 isozyme analyses에서와는 달리 RAPD analyses에서는 많은 수의 strain-specific band들이 얻어질 수 있고 이것이 두 방법간의 유사성을 관찰하지 못하게 하는 원인이라고 보고하였다.

• Applied Biological Chemistry
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• 제20권2호
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• pp.247-254
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• 1977

야생대두(野生大豆)(Glycine ussuriensis, 13개(個) 국내수집종(國內蒐集種)) 종실단백질(種實蛋白質)의 acylamide gel 전기영동양상(電氣永動樣相)을 Glysin gracilis 및 광교(光敎)(G. max)와 비교(比較)하였다. 야생대두(野生大豆)의 단백질함량(蛋白質含量)은 평균(平均) 50%로 gracilis (46%)나 광교(光敎)(45%)보다 높았으며 종실중(種實重)(2.1g/100립(粒))은 gracilis의 1/3, 광교(光敎)의 1/9이었다. 전기영동(電氣泳動)된 단백대(蛋白帶)는 야생대두(野生大豆)에서 총(總)16개(個)였으며 이중 3개(個)($R_m$ 0.09, 0.59 및 0.84)는 86개(個)의 재배대두(栽培大豆)에서 발견(發見)되지 아니한 야생대두고유대(野生大豆固有帶)였다. 재배대두(栽培大豆)는 17개단백대중(個蛋白帶中) 4개(個)의 고유대(固有帶)($R_m$ 0.35, 0.45, 0.50, 및 0.77)를 갖으며 gracilis는 광교(光敎)가 갖는 모든 단백대외(蛋白帶外)에 2개대(個帶)를 더 갖는데 그 중하나가 야생대두고유(野生大豆固有)의 것이어서 중간형(中間型)임을 나타내고 있다. 야생대두(野生大豆)의 1개단백대중(個蛋白帶中) 제3대(第3帶)(32%)가 가장크고 다음의 제1대(第1帶)(28%) 및 제5대(第5帶)(13%)로 이들이 양적(量的)으로 주대(主帶)를 이루었다. 전기영동양상(電氣泳動樣相)은 정성적(定性約)으로 4군(群), 반정량적(半定量的)으로 6군(群), pattern similarity법(法)이나 상관도법(相關度法)으로는 기준(基準)에 따라 $2{\sim}4군(群)으로 분류(分類)되었으며, 이들 방법(方法)은 속리산(俗離山) 채집종(採集種)을 별개군(別個群)으로 분리(分離)하는 것만 동일(同一)하였으나 상관도법(相關度法)이 가장 좋을 것으로 보였다. 종실단백질함량(種實蛋白質含量)은 영동양상(泳動樣相)과는 무관(無關)하였으며 다만 제1단백대(第1蛋白帶中)(28%)다만 5%수준(水準)에서 유의정상관(有意正相關)을 보여 종실단백질(種實蛋白質) 함량결적(含量決的)에 중요역할(重要役割)을 하는 것으로 보였다.

• 환경생물
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• 제37권3호
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• pp.317-334
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• 2019

The present study was conducted to investigate the differences in managed farming practices, including low-intensive farming, duck farming, and golden apple snail farming, in a rice ecosystem by comparing terrestrial arthropod communities. A total of 75 species from 70 genera belonging to 43 families in 11 orders were identified from 9,622 collected arthropods. Araneae, Hemiptera, and Coleoptera were the richest taxa. Collembola was the most abundant, followed by Diptera, Hemiptera, and Araneae. Bray-Curtis similarity among the farming practices was very high (76.7%). The biodiversity of each farming practice showed a similar seasonality pattern. The richest species group was the predators, followed by the herbivores. The species richness and diversity of ecologically functional groups among the farming practices were not statistically significant, except for the abundance of predators in golden apple snail farming. The biodiversity seasonality of ecological functional groups in each farming practice showed similar patterns. The biomass of Araneae, Hemiptera, Coleoptera, and Diptera was greater than the other taxa, in general. The biomass of each ecological functional group showed little difference and the biomass fluctuation patterns in each farming practice were almost the same. Collectively, the community structures and biodiversity of terrestrial arthropods among the farming practices in the present study were not different. The present study may contribute to sustain rich biodiversity in irrigated rice fields and to advanced studies of food webs or energy flow structures in rice fields for ecological and sustainable agriculture.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권5호
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• pp.323-326
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• 2002

To detect whole ammonia-oxidizing bacteria in the activated sludge, group-specific primers targeting the 16S-rRNA gene of ammonia-oxidizing bacteria were used. The electrophoresis pattern of the PCR products seemed to produce a single band of approximately 1.0 k bp for the bacteria in activated sludge and Nitrosomonas europaea. No band was observed for nitrite-oxidizer Nitrobacter winogradskyi and heterotrophs such as Pseudomonas putida. Then direct measurement of the PCR product was made by fluorometry using the reagent Hoechist 33258, so that the fluorescent intensity was in proportional to the cell number of the sample up to 240. Total time required for the test was about 4 h including DNA extraction. The DNA fragments produced were cloned and their sequences showed high similarity to those of Nitrosomonas spp. This study showed the feasibility to detect ammonia-oxidizing bacteria and to esti-mate their population rapidly for the control of the nitrogen elimination process.

• The Plant Pathology Journal
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• 제36권4호
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• pp.364-377
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• 2020

Sugarcane is an important sugar crop contributes more than 80% of world sugar production. Mosaic, leaf fleck, and yellow leaf (YL) are the major viral diseases affecting sugarcane, amongst YL occurrence is widely reported in all the sugarcane growing countries. It is caused by Sugarcane yellow leaf virus (SCYLV) and detailed works were done on complete genome characterization, transmission, and management. However, in countries like Egypt, South Africa, Cuba, Mauritius and Hawaii, the disease was reported to the cause of sugarcane yellow leaf phytoplasma (SCYP) and/or SCYLV as single/combined infections. Hence, we have investigated in detail to identify the exact Candidatus phytoplasma taxon associated in Indian cultivars affected with YL. The sequencing results and the restriction fragment length polymorphism pattern of the PCR products using the universal phytoplasma primers confirmed presence of sugarcane grassy shoot (SCGS) phytoplasma (16SrXI group) in the YL-affected plants. Mixed infection of SCYLV and SCGS phytoplasma was estimated as 32.8% in YL affected plants. Evolutionary genetic relationship between SCYP and SCGS phytoplasma representatively taken from different countries showed that SCYP from South Africa and Cuba were diverged from others and had a highest similarity with SCGS phytoplasma. Although we wanted to identify SCYP from YL affected Indian sugarcane cultivars, the study clearly indicated a clear absence of SCYP in YL affected plants and we found SCYLV as the primary cause for the disease.

• 말소리와 음성과학
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• 제6권2호
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• pp.123-131
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• 2014

The purpose of this study is to investigate the comprehension and production of various tense markings in Korean-speaking children with and without language delay. Thirty children with language delay(LD) and 30 typically developing(TD) children participated in the study. In each group, half were at the age of 4-years and the other half at 7-years. In both the comprehension and production task, 28 verbs containing four types of tense markings were used: past tense '-et ta', two present progressives '-ko itta', '-enta', and future tense '-elyeko hanta'. In the comprehension task, the children were presented with three printed still-scenes of video recording of a verb action, each representing future, present progressive, and past tense of the verb, respectively. Then they listened to the action verb with one of the 4 tense markings and had to pick the scene that matched the verb tense. In the production task, the children were given one of the three scenes and asked to produce the verb with appropriate tense marking. In both tasks, the LD children performed significantly worse than the TD children, and the older children performed significantly better than the younger children. Interestingly, the pattern of performances across different types of tense markings at the two language-age levels were closely similar in LD children and TD children. This similarity of groups seemed stronger in the comprehension task than the production task.

• 한국패션뷰티학회지
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• 제3권2호
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• pp.65-71
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• 2005

The aim of this research is to investigate the demographic characteristics of the purchase tendencies and shopping trends amongst female golfers and how these are influenced by their life style and to analyse their selection criteria for purchases of golf-related items. The research methodology was through the use of questionnaires, completed by female golfers in Gyeongnam. The results are as follows: life style trends of male golfers were analysed to be categorized into one of the following: the shopping-addicted, fashion-conscious, rationalist and family oriented spenders. The characteristics of these categories are described as one of the following: utilitarian-complacent, rationalist, self-worshipping, inconsiderate. The demographic characteristics showed notable variations only in age differences. The obtained results show that the influences of the variables are minimal and there was no notable correlation. Significant differences were observed from one life style group to another, in selection criteria for purchase, which mainly depended on style, design, colour, pattern, designer-label, co-ordinated looks, similarity, ease of maintenance and functionality. Comparisons were made between the previously categorized life-style-groups and notable differences were present in such characteristics as ostentatious, trendy, aesthetically pleasing and functional.

• 정보처리학회논문지D
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• 제11D권5호
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• pp.1205-1212
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• 2004

전자상거래 시스템의 보급이 활성화되기 시작하면서, 사용자의 필요와 욕구에 밀착한 적응형 전자상거래 에이전트의 필요성이 증대되고 있다. 이와 같은 적응형 전자상거래 에이전트는 사용자의 행위를 모니터하고 자동 분류하여 사용자의 취향을 학습하는 기능을 요하게 되었다. 이러한 기능을 가지는 적응형 전자상거래 에이전트를 구축하기 위해서, 본 논문에서는 사용자 개인의 관심정보와 선호하는 상품에 대한 호감도를 고려한 적응형 전자 상거래 에이전트 시스템을 제안한다. 제안하는 시스템은 사용자의 구매 행위에 적응력을 가질 수 있도록 보다 정확한 사용자 프로파일을 구축하고, 이와 같은 사용자 프로파일을 기반으로 사용자에게 불필요한 검색과정 없이 필요한 상품 정보를 제공 할 수 있도록 한다. 본 시스템에서는 모니터링을 통하여 사용자 의도를 파악하는 모니터 에이전트, 사용자의 행동성향을 학습 한 후 행동 패턴이 유사한 그룹을 참조하는 유사도 참조 에이전트, 사용자의 행위의 변화에 따른 개인화된 행동 DB를 구축할 수 있는 관심 추출 에이전트로 구성하였다.