• Korean Journal of Fisheries and Aquatic Sciences
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• v.4 no.3_4
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• pp.113-120
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• 1971

In 1971, about 150,000 fingerlings of grass and silver carps were produced and distributed from the Fishculture Laboratory of Pusan fisheries College. The adults Were those transplanted from Japan in 1963. Findings during the process of this production are summarized as following : 1. Brood fish must be handled with a great care during the catching, hormone injection and maturity inspection so as the fish are not injured, otherwise, the eggs would not mature perfectly. Scale fall also affects significantly. 2. The amount of pituitary to be injected is preferable to be 2 to 3 times or more in donor's body weight to obtain good results. 3. The eggs should be spawned or stripped and inseminated as soon as mature. If the spawning is delayed the eggs become overmature and the hatching rate decreases significantly. 4. The water once used for the incubation of eggs should not be reused. The eggs under hatching process were melted away when water once used for incubation was reused. 5. A great care must be paid to keep water in quality when the net cage culture system is employed for raising fry or early fingerlings of Chinese carps. The best method to keep water in quality is to supply water in the cage continuously through a pipe or hose. 6. Heavy outbreaks of Spirogyra occured when clear well water was supplied into the cage, and a great number of fry or fingerlings were trapped or entangled resulting in a significant decrease of fish under rearing. It was prevented when moderately bloom-ed pond water was supplied into the cage. 7. Silver carp fry are usually captured and transported in the cool season because they can not be handled in the warm season owing to a high mortality, but cage cultured small size fingerlings ranging from 1.5 to 2 cm in total length are easily handled and transported even in the hot summer season, thus it enables the fish farmers to start growing them one season earlier.

• Parasites, Hosts and Diseases
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• v.27 no.1
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• pp.9-14
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• 1989

Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and centrifuging 24-week old adult worms at 10,000 rpm for 60 minutes in phosphate buffered saline (PBS, PH 7.2) containing: Phenyl methyl sulfonyl auoride (PMSF). Gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) was performed and blotted electrophoretically onto a sheet of nitrocellulose paper. The sheet was cut into strips and exposed to sera diluted 1 : 200 with PBS. SDS-PAGE showed 26 protein bands ranging 229 to 10 kDa. Of them 229, 91, 60, 50, 35∼31, 27, 25, 21, 17, 11 and 10 kDa components showed positive reaction with serum antibody of patients with p. westermani. Sera of patients infected with Clcnorchis sinensis reacted with 35∼31, 19, and 11 kDa bands. Human sera from cysticercosis and diphyllobothriasis cases showed non-specific cross reactions with 229, 35∼31, 27, 25 and 17kDa bands. Protein bands of 91, 60, 21 and 10kDa showed strong positive reaction without cross reactions with sera from other helminthic infections.

• Journal of the korean academy of Pediatric Dentistry
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• v.46 no.4
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• pp.382-391
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• 2019

This study was aimed to assess the new trial for minimal cavity preparation in composite restoration combined with resin infiltration, focusing at application sequence. 32 human primary molars with early carious lesions around small cavity were selected and randomly divided into two groups, according to the sequence of cavity preparation (P), composite filling (F) and resin infiltration (I) as IPF and PFI group. Each group was assessed about amount of tooth reduction, features of resin infiltration, and marginal leakage around restoration. Amount of tooth reduction evaluated using micro-CT was decreased compared with the original lesion size in both groups. Features of resin infiltration were verified under confocal laser scanning microscopy. In both groups, infiltrant resin was found on all around the composite and maintained in spite of extent of decalcification even after artificial caries induction. Marginal micro leakage assessed with silver nitrate immersion and micro-CT was found more frequently in PFI group. The technique combining resin infiltration and composite restoration might ensure better adhesion prognosis as applied by the sequence of resin infiltration, cavity preparation, and composite filling. This new trial was thought meaningful in minimizing the cavity size and contributing to minimal invasive dentistry.

• Tuberculosis and Respiratory Diseases
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• v.48 no.2
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• pp.191-202
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• 2000

Background: Bronchial asthma is characterized by airway hyperresponsiveness(BHR) and inflammation. The cyclooxygenase(COX) is believed to be one of the important enzymes in these inflammatory reactions. Recently, the COX was divided into two isoforms, COX1 and COX2. COX2 is induced by lipopolysaccharide and some cytokines at the inflammation site. Prostaglandin E2(PGE2), produced from COX2, may affect airway inflammation. The purpose of this study is to evaluate the effect of COX2 inhibitor on COX2 expression, plasma PGE2, airway resistance and histologic finding in an animal asthma model. Methods : Sprague-Dawley rats were divided into 3 groups. The normal control group did not receive any treatment, but the asthma control group was sensitized by ovalbumin but not treated with the COX2 inhibitor(nimesulide, Mesulid$^{(R)}$). The treatment group was sensitized and treated with nimesulide. Specific airway resistance(sRaw) before and after nimesulide ingestion was investigated. The PGE2 level in the plasma was examined and COX2 immunogold-silver stain on lung tissue was performed. Results: sRaw and eosionophilic infiltration on airway, which increased in the asthma control group, was compared to normal control(p=0.014). However, there was no difference in eosinophilic infiltration between asthma control and treatment groups(p=0.408) and no difference in COX2 expression on bronchiolar epithelium among the three groups. Plasma PGE2 levels were not statically different among the three groups. Conclusion: The role of COX2 in the allergen-induced BHR was not significant The effect of nimesulide was not observed on BHR, COX2 expression, and plasma PGE2 level. Therefore, COX2 may not be a major substance of allergic asthma.

• Applied Microscopy
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• v.10 no.1_2
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• pp.7-17
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• 1980

Electron microscope radioautography introduced by Liquier-Milward (1956) is now used routinely in many laboratories. Most of the technical difficulties in specimen preparation have been overcome. This method is modified from loop method for improvement of EM radioautographic techniques. The advantages of this method are: 1. the use of single specimens on small corks and of a large wire loop, allows the experimenter to avoid the blemishes in the membrane; 2. the surfactant dioctyl sodium sulphosuccinate is added to diluted ILford L4, thus greatly prolonging the period of time over which good emulsion layers can be made; 3. corks can be handled in perspex holder which allows about 20 specimens to be developed simultaneously. The steps of the method comprise: 1. Cut ribbons of ultrathin sections of silver interference colour 2. Pick them up on formvar-coated 200 mesh grids 3. Prestaining of tissues 4. Coat the specimens with a thin layer of carbon by evaporation (30-60A) 5. Mount the specimens on corks (about 1cm apical diameter) using double-sided scotch tape 6. Emulsion coating; a. Take a 250m1 beaker, place it on the pan of a sliding weight balance and weigh it. Add 10 grams extra to the beam. Add pieces of ILford L4 emulsion to the beaker until the balance is swinging freely. Add the 20ml of distilled water that was previously measured out. b. Surfactant dioctyl sodium sulphosuccinate is added to diluted ILford L4. 7. Prepare a series of membranes of gelled emulsion with the wire loop and apply one to each cork-borne specimen. 8. Put the specimens away to expose by pushing the corks into short length of PVC tubing, each tube having a small hole in the side 9. Place the tubes in small boxes together with silica gel. 10. Exposure 11. Developer - Kodak Microdol X for 3 minutes 12. Fixer - A perspex holder can be manufactured which allows 20 specimens to be developed simultaneously. 12. Fixer - 30% sodium thiosulfate for 10 minutes 13. Examination with Siemens Elmiskop 1A electron microscope

• Ocean Science Journal
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• v.42 no.1
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• pp.41-48
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• 2007

We investigated the temperature and salinity effects on the major fish species in the wintering grounds based on trawl surveys and oceanographic observations in the southwestern waters of Korea during March-early April in 2002-2003. The influence area of warm Kuroshio water was limited to the southwestern area of Korea in 2003 with a range of $7.7-16.3^{\circ}C$, 32.54-34.70 of salinity, wider than that of 2002. The number of fish species and density of major fish species in 2003 were higher than in 2002. Geographical estimation showed high proportions of species number and catches in the areas around Jeju Islands, southwestern waters and the southeastern coast of Korea. Five species; silver pomfret (Pam pus echinogaster), hairtail (Trichiurus lepturus), anchovy (Engraulis japonicus), Small yellow croaker (Larimichthys polyactis) and yellow goosefish (Lophius litulon) were most abundant, composing above 60% of the total catch in 2002 and 2003. More than 50% of catch in the major fish species were mostly distributed in the range of $9.5-11.0^{\circ}C$ of temperature and 33.1-33.9 of salinity. Non-parametric estimation for the major species showed the 1st mode around $10^{\circ}C$ and the 2nd mode at $8-9^{\circ}C$ in 2002 and $11-14^{\circ}C$ in 2003. Among major fish species, hairtail was principally composed of juveniles, and larger individuals were caught in southeastern waters. These results are considered to be helpful for the area-based fishery management strategy for the wintering grounds of the Yellow Sea and coastal waters of Korea.

• Journal of the Korea Fashion and Costume Design Association
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• v.13 no.4
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• pp.127-138
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• 2011

The style which is created by grafting the characteristics of goth onto haute couture designer works is called 'Haute goth'. The purpose of this study is to analyze the modem Goth fashion style based on Goth's historical background and characteristics. Also, I investigated the aesthetic characteristics of haute goth style appeared from the work of haute couture designers. Goth means a member of East Germanic people who invaded and settled in the Roman Empire from the 3rd to the 5th century. Goth style had been developed to the medieval European architecture style or the literature style of 18th~19th centuries and later it was reappeared in the gothic band in the late 20th century. Goth fashion shows the characteristics of the medieval times and Victorian times mixed with punk and fetish style. Goth fashion is expressed with the images of fear, darkness, mystique, dandy and eroticism. 'Dark esthetics' is realized through black clothes, pale complexion and silver accessories. Many designers like Alexander McQueen and John Galliano designed 'haute goth fashion' in various ways. The characteristics of 'haute goth' can be summarized in four things. It creates new images, seeks various changes and pursuits newness. It upgrades a street look style gothic fashion to a luxury high fashion and the creative design with artistic value. It also shows an experimental sprit by using a unique shapes, silhouettes, different materials and extreme images or creating new combination through the mismatch of opposite objects or emotions. Although it is rooted in the past because of the influences by late medieval times, Victorian times and Edwardian times, it displays enough of future-oriented historicism designs.

• Journal of Korean Ophthalmic Optics Society
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• v.3 no.1
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• pp.39-73
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• 1998

In the 60's to 70's, frame selection was a purely mechanical consideration almost entirely dominated by the optician and his subjective judgments. Toddy the cosmetic factor is predominant. The variety of frame color, materials, and style means that the main burden of selection has passed to the customer, leaving the optician control a subtle factor often difficult to exert. Common materials include nickel silver, Hi-nickel alloy, bronze, stainless steel, gold, gold plated, gold clad, copper beryllium, titanium and sometimes aluminium. In manufacturing of metal frames with the materials, even though fashion is the stimulant of consumer demand, all the metal frame for the prescription to refractive correction should be designed correctly styled eyewear which is cosmetically pleasing, functional correct, physically comfortable, mechanically and structurally perfectible, and temperamentally acceptable.

• The Research Journal of the Costume Culture
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• v.17 no.1
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• pp.76-89
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• 2009

Popular music unites people who have different genders, ages, locals, and values. Through the popular music, youth interchange their emotion and create their fashion. Fashion and music formed the twin pillars on which the first historically significant youth sub-culture was built. Glam rock and Punk rock which won popularity in the 1970s influenced the fashion of the times. The purpose of this study is to compare Glam and Punk make-up which appeared as a symbol of the rebel against an older generation. The methodology utilized in this study was the analysis of materials from the survey through the literature, internet sites, and visual references. The results of this study are as follows: In face make-up, Glam used a white base to appear cosmic; on the other hand, Punk used a white base to appear pale and horrid. In eye make-up, Glam used black, pink, and blue eye shadow, and accented the eye by using black eye lines. Punk used black eye shadow, and drew black circles or squares around eyes. In lip make-up, Glam used pink, purple, red, and black lip color and silver and pink lip gloss to glitter. Punk used red and black lip color to appear horrid. In body make-up, Glam used glitter powder to appear with a bright skin. Punk drew tattoos and horror patterns in their body. The common themes of Glam and Punk make-up were rebellion, androgyny, and the beauty of ugliness as well as a self-created persona.

• Journal of agricultural medicine and community health
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• v.42 no.2
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• pp.87-96
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• 2017

Objectives: We studied the relationship between health behavior and subjective unhappiness in high school students. Methods: Using 27,097 responses from the 2015 Korea Youth Risk Behavior Web-based Survey for general high school students. we analyzed by multiple logistic regression based on the complex sample design Results: Unhappiness was positively related with the low economic status, smoking, drinking, fast foods intake, and negatively related with fruit intake in results of multiple logistic regression. Conclusion: Health behaviors have a significant impact on the unhappiness of high school students. Therefore, in-depth research and policies to decrease unhappiness of high school students through health promotion are required.