• Title/Summary/Keyword: Silver College

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Dye-silver double staining method for proteins in SDS-polyacrylamide gels using a dye as a silver sensitizer

  • Jin, Li-Tai;Hwang, Sun-Young;Yoo, Gyurng-Soo;Choi, Jung-Kap
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.145.2-145.2
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    • 2003
  • We have developed a silver staining method using a dye as a silver sensitizer. Dye staining is performed in combination with silver nitrate staining. Dye-silver staining shortens the time of silver staining (~1 hr) and improves the sensitivity better than that of silver diamine stain (1-10 ng) or comparable to that of silver nitrate stain with glutaraldehyde as a silver sensitizer. In dye staining (silver sensitizing step), it has been proven that the sensitivity is at least 4 times comparing with that of CBBR stain and staining time is about 45 min. (omitted)

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Antimicrobial Effects and Mechanism(s) of Silver Nanoparticle (은나노 입자의 항균작용과 작용기작)

  • Hwang, In-Sok;Cho, Jae-Yong;Hwang, Ji-Hong;Hwang, Bo-Mi;Choi, Hye-Min;Lee, June-Young;Lee, Dong-Gun
    • Microbiology and Biotechnology Letters
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    • v.39 no.1
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    • pp.1-8
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    • 2011
  • The antimicrobial effects of silver (Ag) ion or salts are well known. Recently, silver nanoparticle is attracting an interest in a wide variety of fields since it has been known to be safe and effective as an antimicrobial agent against a broad spectrum of microorganisms. Although silver nanoparticle has been applied to various kinds of products owing to its potent antimicrobial activity, the effects of silver nanoparticle on microorganisms and antimicrobial mechanism have not been revealed clearly. In this paper, we summarized the characteristics, antimicrobial activities and mechanisms, cytotoxicity and applicability of silver nanoparticle.

Chronic Renal Failure in Russell-Silver Syndrome (Russell-Silver 증후군에서의 만성 신부전)

  • Ahn, Yo-Han;Lee, Se-Eun;Kang, Hee-Gyung;Ha, Il-Soo;Cheong, Hae-Il;Choi, Yong
    • Childhood Kidney Diseases
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    • v.13 no.2
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    • pp.256-260
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    • 2009
  • The Russell-Silver syndrome (RSS) is a disease characterized by intrauterine growth retardation with preserved head circumference, facial dysmorphism and short stature. Reported renal and urinary manifestations of RSS include horseshoe kidney, renal tubular acidosis, hydronephrosis, ureteropelvic obstruction and vesicoureteral reflux. Here we report a case of end-stage renal disease associated with RSS, which, to the best of our knowledge, has not been reported yet.

Silver Materials Induce Differential Cytotoxicity and Pulmonary Toxicity Based on Size and Shape

  • Pak, Pyo June;Kang, Beob Hwa;Chung, Namhyun
    • Journal of Applied Biological Chemistry
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    • v.58 no.2
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    • pp.113-116
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    • 2015
  • Silver materials may be toxic in humans because they can enter the body and accumulate, typically in the lungs. We hypothesized that the cytotoxicity of naive silver materials is affected by their size and shape. Our in vitro assays revealed that the overall toxicity was in the following order: submicro-particles>wires>micro-particles. These results contrast with previous studies, which showed that silver wires are the most toxic among the three tested materials, possibly due to differences in cell lines. Evaluations of in vivo pulmonary toxicity revealed eryptosis in the cavity lining of the lung sections. The observed eryptosis was consistent with the in vitro results. Our results indicate that silver materialinduced cytotoxicity must be measured and compared using various methods.