• Mycobiology
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• 제38권2호
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• pp.128-132
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• 2010

Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a $100\;{\mu}L$ injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than $2\;{\times}\;10^5$ colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.

• 한국잠사곤충학회지
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• 제4권
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• pp.57-61
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• 1965

인삼물질첨식잠은 체중과 견중이 현저히 무거웠고 견층비율도 좋았으며 일부에서는 용체중의 차이점도 나타났으므로 우량잠품종육성에도 기대되는 바가 있을 것으로 본다.

• 한국잠사곤충학회지
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• 제6권
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• pp.28-30
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• 1966

현재의 우리나라 장려품종을 공시하여 잠용비중에 의한 자웅분리방법을 조사하였든 바 다음과 같은 몇 가지 결과를 얻었다. 1) 현장려품종의 잠용의 비중은 1,000∼l,100 범위내에 속한다. 2) 식염수의 비중이 1,020전후에서 자용은 저부에 가라앉고 웅용은 상층 즉 식염수 표면가까이 뜬다. 3) 식염수의 비중 1,020전후에서는 잠용에 아무런 악영향이 없었을 뿐만 아니라 발아비율과 산란수에 나쁜 영향을 주지 않았다. 4) 식염수 처리가 보다 광범위한 실용형질에 미치는 영향에 관한 시험은 다음 기회로 미루고 잠견절개기의 고안이 선행되어야 한다.

• Journal of Dairy Science and Biotechnology
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• 제35권4호
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• pp.262-265
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• 2017

Bombyx mori (silkworm pupae) is a unique and biologically significant insect, which is a recognized source of high quality protein that provides all the essential amino acids required for human health. Recently, many studies have focused on various biomedical applications of B. mori proteins. The purpose of this study was to manufacture protein-fortified kefir containing different concentrations of B. mori powder according to pH and sensory evaluations. The value of the protein-fortified kefir increased but the pH decreased with increasing incubation time, indicating that the amount of B. mori powder did not affect and pH. Addition of B. mori powder also did not affect the sensory properties of overall acceptability, texture, and color compared to control group without addition of B. mori powder. However, flavor and taste were affected by increasing the amounts of B. mori powder, with a significant difference in both flavor and taste between the control and treated groups (both p<0.05). There was no significant difference in overall acceptability, texture, and color. Further studies are needed for producing kefir as a dietary supplement utilizing the functional properties of B. mori.

• International Journal of Industrial Entomology and Biomaterials
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• 제19권1호
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• pp.175-180
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• 2009

Recently, as the male silkworm pupae, bee pupae have the potential that strengths men's vitality on vascular endothelial nitric oxide in endothelial cells. Especially we prepared alcohol extract of pupae of bumblebee, native bee named Hobakbul, Bombus ignitus. The alcohol extract of pupae of B. ignitus was administered to rats at doses of 0, 0.04, 0.2, 1 or 2 g/kg as a single oral dose. There were no observed clinical signs or deaths related to treatment in all the groups tested. Therefore, the approximate lethal dose of the alcohol extract B. ignitus pupae was considered to be higher than 2 g/kg in rats. Mild decreases in body weight gain in male were observed dose-dependently within B. ignitus pupae alcohol extract treated groups in dose response manner over 2 weeks. Throughout the administration periods, no significant changes in diet consumption, ophthalmologic findings, clinical pathology (hematology, clinical chemistry and coagulation) or gross pathology were detected. Minor changes in male and female rats were found in hematological parameters for all or partial of B. ignitus pupae extract treated groups but all the changes observed were within the physiological range. From these results, it was concluded that there was no-evidence of specific toxicity related to the ingestion of alcohol extract of B. ignitus pupae.

• 한국버섯학회지
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• 제3권4호
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• pp.133-139
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• 2005

경제적 부가 가치가 높은 붉은자루동충하초의 안정적 대량생산 기틀을 마련하고자 자실체 증식 특성 및 자실체 성분 분석을 한 결과를 요약하면 다음과 같다. 1. 붉은자루동충하초의 감염률은 관행인 누에 5령 기잠시 분무 접종하는 방법보다 번데기 시기에 주사접종 하는 방법이 높았다. 2. 붉은자루동충하초의 자실체 발생은 온도 $21{\sim}25^{\circ}C$, 습도 91% 이상, 광 1500 lx 이상에서 양호하였다. 3. 붉은자루동충하초의 유리당 함량은 Glucose가 7.9 mg으로 가장 많았으며, 다음이 Sucrose 이었으며 Glycerol과 Mannitol은 미량 검출되었다. 4. 붉은자루동충하초의 누에 자실체에 함유된 아미노산의 총 함량은 $34.6{\mu}mole/g$이며, 16종의 구성 아미노산중 Arginine이 15.6%로 가장 높았으며, Lysine이 3.4%로 가장 낮게 분포하였다. 5. 붉은자루동충하초의 누에 자실체 유리 지방산 조성은 Oleic acid 함량이 가장 많았으며, Palmitoeic acid는 소량 함유되어 있었다. 6. 붉은자루동충하초의 누에 자실체 유기산 조성은 Citric acid가 가장 많았으며, Malic acid와 Propionic acid는 미량 존재하였다.

• 한국잠사곤충학회지
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• 제40권2호
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• pp.176-179
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• 1998

Injection method of Paecilomyces japonica to Antheraea pernyi pupal body was investigated on feasibility for production of the corresponding vegetable wasp and plant worm. Infection rate of P. japonica to the pupae was 100%, and also fruiting body formation rate was 97%. The initial pupal body weight of 9.38 g befor injection of P. japonica to the pupae was finally reduced to 7.87g after two weeks in vivo culture. From these results, the injection method to pupal body will be useful for the production of Antheraea vegetable wasp and plant worm in future.

• International Journal of Industrial Entomology and Biomaterials
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• 제12권1호
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• pp.45-49
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• 2006

The present investigation was conducted to know the effects of host age on development and morphological characteristics of Nesolynx thymus, a gregarious ectopupal parasitoid of the uzi fly, Exorista bombycis, a serious en do-larval parasitoid of the silkworm, Bombyx mori L. For experimentation, 3 - 11 day-old pupae of E. bombycis were exposed to 2 day-old adults of N. thymus at $25{\pm}2^{\circ}C\;and\;60{\pm}10%$ RH. A highly significant negative correlation between host age and the following development and morphological characteristics of the parasitoid was observed: number of pupae parasitised, total adult recovery, sex ratio and recovery, longevity, body length, wing span, abdomen length, and abdomen width of females. The findings of the present study clearly demonstrate that various development and morphological parameters of N. thymus are influenced by the age of E. bombycis pupae.

• 한국균학회지
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• 제30권1호
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• pp.11-17
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• 2002

공시균주인 EFCC C-251, EFCC C-252, EFCC C-1092 균주 중 현미(60 g)와 누에 번데기(30 g)를 혼합한 배지에서 EFCC C-252 균주가 자실체가 잘 형성되어 이 균주를 가지고 본 연구를 수행하였다. 자실체 형성 최적온도는 $25^{\circ}C$이었으며 광은 형광등으로 조명도는 300 lux이었다. 기본배지에 lactose, ammonium sulfate와 $KH_2PO_4$를 첨가한 배지에서 균사의 생육은 좋았으나 자실체 형성은 기본배지에 현미(20 g)를 첨가한 배지에서 좋았다. 현미만으로는 70 g를 넣은 배지와 누에 번데기만은 80 g를 넣은 배지에서 에서 자실체의 형성이 좋았다. 현미와 누에번데기를 같이 넣었을 때는 현미 60 g에 누에번데기 40 g에서 다른 비율배지에 비해 자실체 형성이 좋았다. 관수는 2일 간격으로 한 처리구가 좋았으며 액체종균의 접종량은 $5{\sim}25\;ml$까지는 자실체 생육이 비슷함을 보이다가 접종량이 증가할수록 자실체 생육이 저조하였다.

• Mycobiology
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• 제39권3호
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• pp.158-163
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• 2011

This study was conducted to confirm the possible use of female Yangwonjam as a host for synnemata production of Isaria tenuipes in eight local areas in Korea. Silkworm pupation rate, infection rate and synnemata characteristics of I. tenuipes were examined. Normal silkworms had a higher pupation rate than silkworms inoculated with I. tenuipes. The pupae survival percentage of normal silkworm in cocoons was 92.5~97.6%, whereas it ranged from 91.1~95.6% in silkworms sprayed with I. tenuipes. Female Yangwonjam showed the highest survival percentage at 97.6% among the silkworm varieties tested. I. tenuipes infection rate of larvae of 5th instar newly-exuviated silkworms was 89.2~90.7% in the spring rearing season and 98.2~99.3% in the autumn rearing season. Synnemata production of I. tenuipes was excellent in female Yangwonjam with an incidence rate of 98.0% followed by male Yangwonjam (94.1%) and Baegokjam (93.3%) in the spring rearing season. Synnemata living weight ranged from 1.44~0.94 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight (1.44 g) in the spring rearing season. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and were similar in shape and color to wild synnemata collected in Korea.