• 한국잠사곤충학회지
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• 제17권1호
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• pp.31-34
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• 1975

춘채 휴면잠종을 산난후 약 50일째에 전기충격을 줌으로서 부화의 가능성여부를 실험한 결과 다음과 같은 결론을 얻었다. 1. 휴면란에 대한 전기충격은 배자의 발육을 촉진시켰다. 2. 전기처리시간은 5초에서 비교적 부화성적이 좋았다. 3. 침산처리일로부터 약 15～22일 사이에 산발적으로 부화가 시작되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.79-87
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• 2004

The silkworm rearing and growth parameters of 63 multivoltine silkworm accessions under extended period of egg preservation at 5$^{\circ}C$ from 30 days to 45 days were studied. The results indicate that, nine accessions did not respond to extended period of egg preservation at low temperature and the remaining 54 accessions responded to the treatment and three rearings were conducted for comparision with the control; to estimate the effect of prolonged egg preservation at low temperature. The non-parametric tests statistics (Wilcoxon tests) was adopted for comparing the mean performance of treated batches (45 days) over the control (30 days). Highly significant variability was found among the accessions for all the parameters under study. The genetically controlled morphological characters were not altered in the treated batches, which were found to be on par with that of control. However, the total larval duration varied significantly over the control in 51 accessions. Similarly, the fifth age larval duration of 27 accessions showed decreasing trend compared to control. Altogether 41 accessions were found to be tolerant to long-term cold preservation upto 45 days, without showing any significant variation for morphological as well as essential quantitative traits. These accessions may be recommended for long-term egg preservation schedule up to 45 days, which will reduce the cost of conservation of these silkworm germplasm.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권1호
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• pp.45-50
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• 2007

Ethanol extract of 20 botanicals in 3 concentrations i.e., 2.5%, 5% and 10% were evaluated for their efficacy to improve reproductive performance of mulberry silkworm (Bombyx mod L.) through feeding botanical enriched mulberry leaves during $2^{nd}$ day of $4^{th}$ age as well as $1^{st}$ and $3^{rd}$ day of $5^{th}$ age larvae. The preliminary screening was done by ranking the botanicals based on influence in most important reproductive contributing parameters such as single cocoon weight, effective rate of rearing, pupation rate, fecundity and recovery of eggs/kg cocoon. Five botanicals viz., Asparagus recemosus, Achyranthes aspera, Tribulus terrestris, Withania somnifera, Parthenium histerophorus ranked first were further evaluated at 8% 5% and 3% concentrations to confirm their effectiveness and standardize effective concentration. Among the rearing parameters, mature larval weight, shell ratio and number of cocoons/kg were not significantly influenced by the treatments. However, reproductive parameters such as effective rate of rearing (wt), pupation rate (%), fecundity (No.) and egg recovery (g)/kg cocoon were significantly (P<0.05) influenced by the botanicals. Higher fecundity and egg recovery were obtained on treatment with Withania somnifera irrespective of concentrations. However at 5% concentration, pupation rate, fecundity as well as egg recovery were found significantly higher than that of other concentrations, confirming its effectiveness for improving reproductive efficiency.

• International Journal of Industrial Entomology and Biomaterials
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• 제34권1호
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• pp.11-15
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• 2017

We constructed the fibroin H-chain expression system to produce enhanced cyan fluorescent proteins (ECFP) in transgenic silkworm cocoon. Fluorescent cocoon could be made by fusing ECFP cDNA to the heavy chain gene and injecting it into a silkworm. The ECFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the ECFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworms. The EGFP fluorescence became visible in the ocelli and in the central and peripheral nervous system on the seventh day of embryonic development. A mixture of the donor and helper vector was micro-injected into 1,020 Kumokjam, bivoltin silkworm eggs. We obtained 6 broods. The cocoon was displayed strong blue fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the ECFP fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

• 한국잠사곤충학회지
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• 제4권
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• pp.41-46
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• 1965

개량족과 회전족에 각각 300두의 숙잠을 상족시키고 이것을 4구로서 설치하여 그 후의 용체 아 산란에 대하여 조사하였다. 1) 개량족에서는 횡작견이 63％에 대하여 종작 사작은 37％나 많이 나타났다. 2) 용체이상은 개량족에서 많이 나타나 그 후의 모든 형질에 불량한 결과를 초래하였다. 3) 사용에서도 개량족이 많다는 것은 종작이라는 그 현상이 잠용에 어떠한 불리한 생리작용을 주는 것이라고 생각된다. 4) 발아상황은 회전족이 확실히 많다. 5) 정상란아의 수는 회전족이 많다. 6) 산란수에 있어서도 회전족이 많고 체내잔란수는 개량족이 많았다. 이것은 회전족의 쪽이 배란을 더 많게 하고 있다는 결론이라고 할 수 있다. 7) 1000류에 대한 산란중은 회전족이 훨씬 많다. 지수로 표시하면 회전족 100에 대하여 개량족은 85가 되므로 실지 단위생산량에 있어서 회전족이 훨씬 유리하다. 그리고 횡작, 사작, 종작의 별과 또 회전족과의 산란중 비교에서 분산분석결과는 종작 사작은 가장 적고 따라서 이것이 많이 생기는 개량족에서는 회전족에 비하여 생산량이 적어진다는 이유도 여기에 있는 것이다. 8) 경제적으로 타산할 때 회전족은 값이 고가이나 단위 잠종생산량이 많으므로 족대를 지출하고도 더 이익이 많은 것이다.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권1호
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• pp.75-78
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• 2007

To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp20.8A, which was expressed at a high level in diapausing eggs that had been incubated at $25^{\circ}C$ for 30 days after oviposition, whereas, in the eggs exposed to $15^{\circ}C$ for 30 days, $5^{\circ}C$ for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 days after heat-HCl treatment and later at 9 to 10 days after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.83-88
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• 2001

The effect of topical application with 200,400 and 600 $\mu$g/ml 2,4-dichlorophenoxyacetic acid (2,4-D) on the fifth larval stadium of the silkworm, B. mori, was analyzed. Larvae treated during fifth larval stadium enhanced larval, cocoon and adult parameters. The larval period was significantly decreased with increase in silk gland weight at 400 and 600 $\mu$g/ml, male cocoon weight and shel1 weight in all the treated groups and filament length and weight at 200 $\mu$g/ml treated group. Length of the ovariole, eggs per ovariole and hatching percentage increased significantly in all the treated groups when compared with those of the carrier control. This suggests that the plant growth regulator 2,4-D in addition to affecting silk production also affect reproductive performance.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.145-148
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• 2004

As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.

• 한국잠사곤충학회지
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• 제20권1호
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• pp.11-14
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• 1978

가잠에 있어서 식도하신경구의 적출이 잠아내의 조성난내의 아미노산 및 자외선형광물질의 변화에 미치는 영향을 Paper Chromatography법에 의해 조사하여 다음과 같은 결과를 얻었다. 1) 잠아의 조성난내에 존재하는 유리아미노산의 종류나 양이 SG적출구와 대조구사이에 차이를 나타내지 않았다. 2) 형광물질인 Riboflavine이 SG적출구인 나방의 조성난내에는 대조구보다 적었다. 3) Rf식 0.06을 나타낸 주홍색의 형광물질은 SG를 적출한 나방의 조성난내에서는 전혀 검출되지 않았으나 대조구에서는 검출되었다. 4) 원점에 나타난 청색의 형광물질은 SG를 적출한 나방의 조성난내에서만 검출되었으나 대조구에서는 검출되지 않았다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2001년도 제44회 춘계 학술연구 발표자료
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• pp.39-39
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• 2001

No Abstract. See Full-text