• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.2
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• pp.175-180
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• 1984

Lipids in oyster mushroom (Pleurotus florida) were extracted by the mixture of chloroform-methanol (2: 1, v/v) and fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography. Components and fatty acid composition of each fraction were deter- mined by thin-layer and gas-liquid chromatographies. Fresh oyster mushroom contained 0.5% total lipid in which the contents if neutral lipids, glycolipids and phospholipids were 33.8%, 19.7% and 45. 6%, respectively, Triglycerides(38.2%), free fatty acids (20%) and free sterol (10%) were the major components among the neutral lipids. Diglycerides, monoglycerides, sterol esters and three unidentified neutral lipids were the minor components. Major components of glycolipids were steryl glycosides(35.9%) and esterified steryl glycosides (23.7%). Digalactosyl diglycerides, mono-galactosyl diglycerides and two unknown components were also present. Of the phospholipids, phosphatidyl cholines and serines (48.2%), and phosphatidyl ethanolamines(44.4%) were the major components. On the other hand, the major fatty acids of neutral lipids we.e linoleic, palmitoleic, oleic and palmitic acid. Linoleic and palmitic acid were the predominant fatty acids of both glycolipids and phospholipids.

• Korean Journal of Food Science and Technology
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• v.10 no.3
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• pp.361-365
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• 1978

Physico-chemical characteristics of crude oil extracted from Korean Hazel nut were determined and its proximate composition was also analyzed. The proximate composition of Hazel nut was shown to be moisture 4.0%, crude protein 15.5%, crude fat 64%, nitrogen free extractive 11.7%, crude fiber 2.0% and crude ash 2.5%. The content of crude fat in Corylus sieboldiana was about 3% higher than in Corylus mandshurica.. Physico-chemical characteristics of crude oil found were: specific gravity, $0.916(15/15^{\circ}C)$; refractive index, $1.468(15^{\circ}C)$; saponification value, 184; iodine value, 94.5: acid value, 0.2; and unsaponifiable content, 0.25%. The lipid fractions in the crude oil obtained by silicic acid column chromatography were found to be composed of about 97% neutral lipids and about 3% compound lipids. Among the neutral lipids by TLC, triglycerides were 98% as the major components, free fatty acids and free strols were 0.5% and 1.3%, respectively. Esterified sterols were not found. The predominant fatty acids were oleic $(76{\sim}80%)$, linoleic (15%) and palmitic (5.0%), and the P/S ratio was $1.8{\sim}2.8$.

• Korean Journal of Food Science and Technology
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• v.25 no.2
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• pp.160-164
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• 1993

The antioxidant activity of ethanol extracts from defatted perilla flour was investigated by measuring peroxide value of perilla oil during storage at $45^{\circ}C$. The antioxidant activity of ethanol extracts was also compared with BHA, BHT and tocopherol. Anti-oxidant activity of ethanol extracts was also examined in corn oil and lard. The ethanol extracts contents of defatted perilla flour and the original perilla seed were 7.69 and 4.56% respectively. The antioxidant activity of ethanol extracts was superior to that of 0.02% BHT, BHA and tocopherol in the perilla oil substrate, merely in concentration of one-twentieth as much as that contained in original perilla oil seeds. The fractions of non-polar solvent (hexane and chloroform) obtained from silicic acid column chromatography are less effective than that of polar solvent as an antioxidant. Antioxidant activity of partially purified ethanol fraction is slightly inferior to that of original crude ethanol extracts. Ethanol extracts were also effective in corn oil and lard almost same as in perilla oil. The total phenolic compound contents of crude ethanol extracts and partially purified ethanol fraction were 9.3, 6.4%, respectively.

• BMB Reports
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• v.31 no.4
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• pp.328-332
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• 1998

The 8-hydroxyisocoumarin, named Centipedin, which has a significant antibiotic activity, was separated and solubilized with organic solvents, such as diethyl ether from centipede Scolopendra subspinipes multilans L. Koch. The Centipedin was purified by silicic acid column and high S cation exchange chromatography followed by reverse-phase HPLC. It was confirmed that Centipedin has a potent antibiotic effectiveness against Gram-negative Klebsiella pneumoniae ATCC 8308. The results showed that Centipedin blocks both DNA replication and RNA transcription during the growth of this pathogen in vivo. The biosynthesis of antibiotic 8-hydroxyisocoumarin was studied in vivo by feeding $[^{14}C]-labelled$ compound as a precursor to live centipede, in which $[^{14}C]acetate$ was the most efficiently incorporated into the Centipedin within 30 h after injection. Also, in vitro study on the biosynthesis of Centipedin showed that efficient incorporation of $[^{14}C]acetate$ occurred at pH range 5.0-7.0 for 10 h incubation and decreased significantly after then. It is suggested that 8-hydroxyisocoumarin is one of the defense compounds acting on bacterial infection in Scolopendra subspinipes.

• Fire Science and Engineering
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• v.26 no.4
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• pp.18-23
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• 2012

After produce the extracted wood to silicic acid gel, sodium carbonate and silicon dioxide with application of the making method for carbon dioxide, ignition time, ignition temperature, mass loss rate and critical heat flux are measured according to external radiation source (20, 25, 35 and 50 $kW/m^2$). From the results, pressure impregnation wood to use with water glass and carbon dioxide has fire retardant performance at heat flux (less than 20 $kW/m^2$) of Pre-Flashover fires. If we find out the excellent maxing ratio through continuously study, it might be decided to be able to be utilized as fire-retardant wood.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.321-326
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• 1986

Oyster (Crassostrea gigas), arkshell (Anadare(Scapharce) broughtonii) and sea-mussel (Mytilus edulis) were investigated as to their lipid classes. Lipid extracts from shellfishes were fractionated into neutral lipid (NL), glycolipid (GL) and phospho-lipid (PL) by column chromatography with silicic acid. The fatty acid compositions of their lipid classes and lipid fractions were determined by gas liquid chromatography (GLC). Total lipid contents of shellfishes were $3.5\%$ in the oyster, $1.4\%$ in the arkshell, $1.0\%$ in the sea-mussel. The major fatty acids of total lipids were palmitic acid, eicosapentaenoic acid and docosahexaenoic acid in the oyster and the sea-mussel, palmitic acid, oleic acid and eicosapentaenoic acid in the arkshell. The lipid composition of neutral lipid fractions in shellfishes was separated and identified as free sterol, free fatty acid, triglyceride, hydrocarbon and esterified sterol by TLC. Of these classes, triglyceride fraction was most abundant, amounting to 55.6, 77.7 and $60.4\%$ in the three samples mentioned above, respectively. The main fatty acids of glycolipid were palmitic acid, eicosaenoic acid and docosahexaenoic acid in oyster, myristic acid, palmitic acid and palmitoleic acid in the arkshell, docosahexaenoic acid, linolenic acid and palmitic acid in the sea-mussel. The major fatty acids of phospholipid were palmitic acid, eicosapentaenoic acid and docosahexaenoic acid in the oyster and sea-mussel, palmitic acid, eicosapentaenoic acid and erucic acid in the arkshell.

• Journal of Conservation Science
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• v.18 s.18
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• pp.89-96
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• 2006

Surface layers of stone cultural properties including the soluble salt need consolidation because they are mostly very weak. There is a lot of research on the penetration depth of consolidant in stone and the effect of consolidant on mechanical stability of deteriorated structure. But some conservation experiences show that consolidation with silicic acid ester is not successful on salt contaminated stone cultural properties. In this study, in order to assess the influence of soluble salts$(CaSO_4\;2H_2O,\;NaNO_3)$ on the efficiency of consolidation on the deteriorated stone cultural properties(Nationalgalerie, Berlin, Germany) sandstone samples have been soaked with the salts solution. The impregnation of consolidant based on ethyl filicate have been afterwards carried out on these samples. As a result, it confirms that the soluble salts act as a preventer or consolidation. They fill up the pores in the stone and prevent that sufficient amount of consolidant enter deeply into the stone. According to this result, if use silicic ethyl ester as a consolidant for the research object which is built by Nebra sandstone, desalination is necessary before the treatment with consolidant. But it is also reported by other researches that some soluble salts improve the consolidation effect. Therefore it should be necessary to pre-study about salt and its harmfulness before the consolidation treatment. In order to consolidate without the aggravative damage in salt contaminated stone cultural heritage, we must first of all study the relations among salt, stone and consolidant.

• Journal of Ginseng Research
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• v.4 no.1
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• pp.31-39
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• 1980

This study was prepared to observe some enzyme activities in the liver of mice treated with extracts of Ginseng anticancer compound, separated from the petroleum ether extracts by silicic acid chromatography, has the cytotoxic activity against cancer cells. Swiss mice, 72 heads were used (or this experiment and they were divdied into control, test group I and test group If, that test group I was injected crude extract and test group II was injected anticancer compound, while the control group was injected 0.9% NaCl solution. The injections were carried out 1,2,4 and 8 times once a day for 1-8 day, respectively. The liver was removed carefully from the mice at 24 hours after drugs injected, and homogenized at 4$^{\circ}C$ for enzyme study. The activities of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase(GPT) were determined by Reitmen and Frankel method and lactic acid dehydrogenase activity was determined by Wroblewski methods in vitro. The results obtained are summarized as follows; 1. The GOT activity was increased 26%, crude extract and 16%, anticancer compound than those of control at 1st injected groups and decreased gradually according to increase of injection time, at 8th injected groups, the GOT activity was decresed by 16%, crude extract and 12%, anticancer compound. 2. The GPT activity was not changed significantly at 1st and 2nd injected groups, but, at 4th injected groups, the GPT activity was decreased 20%, crude extract and 14%, anticancer compound. While the GPT activity was recovered to normal value at 8th injected groups. 3. At 1st injected groups, the LDH activity was increased 17%, anticancer compound, while those of crude extract was shown normal value. At 2nd injected groups, the LDH activity increased 35yo:, crude extract while those of anticancer compound was showed normal value. And the LOH activity was recovered gradually at 4th and 8th injected groups.

• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.1
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• pp.29-35
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• 1987

The compositional characteristics of polar lipids in the brown and polished adlay were studied. Total lipids of brown and polished adlay were extracted, purified and fractionated into neutral and polar lipids by silicic acid column chromatography. The glycolipid(GL) and phospholipid(PL) of polar lipids were seperated by thin layer chromatography and quantitated by scanner. The fatty acid composition in polar lipids were determined by gas chromatographr. The contents of GL and PL in brown adlay were 5.67% and 1.83%, and their contents on polished adlay were 5.49% and 1.78%, respectively. Of the GL in the brown and polished adlay, monogalactosyl diglycerides+esterified steryl glycosides and digalactosyl diglycerides were the major components, but cerebrosides+steryl glycosides were also found as the minor component of GLs. Of the PLs, phosphatidyl choline and phosphatidyl ethanolamine were the major components. Phosphatidyl inositol, lysophosphatidyl choline and phosphatidyl serine were also present in the PLs. The major fatty acids of GLs were linoleic, oleic and palmitic acids. The fatty acid composition in the PLs were similar to the pattern in the GLs, but PLs contained the lower percentage of linoleic acids than the GLs.

• Korean Journal of Food Science and Technology
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• v.17 no.4
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• pp.248-252
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• 1985

Prunus tomentosa Thunberg seed was investigated to evaluate its possibility for use as food resources of fats and proteins. The seed contained 40.38% of crude fat and 26.59% of crude protein. The lipid fractions obtained by silicic acid column chromatography were mainly composed of 95.49% of neutral lipids, whereas compound lipids were only 4.51%. Among the neutral lipid components by thin-layer chromatography, triglycerides were 89.86%, sterols, monoglycerides, sterol esters, free fatty acids and diglycerides were 4.14%, 2.98%, 1.77%, 1.07%, and 0.18%, respectively. Oleic acid (65.06-66.05%) and linoleic acid (26.56-28.40%) were the main fatty acids in the total lipid, neutral lipid and triglyceride fractions. In the glycolipid and phospholipid fractions, predominant fatty acids were oleic acid (40.55-51.46%), linoleic acid (20.26-30.89%) and palmitic acid (17.64-21.43%). The extractability of salt soluble protein of seed was 60%, and recovery rate of main protein fraction separated by Sephadex G-200 was about 46.5%. The electrophoretic analysis showed 7 bands in seed protein.