BMB Reports
- Volume 31 Issue 4
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- Pages.328-332
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- 1998
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- 1976-670X(eISSN)
Mechanism of Antibiotic Action and Biosynthesis of Centipedin Purified from Scolopendra subspinipes multilans L. Koch (Centipede)
- Kim, Ki-Tae (Dept. of Biochemistry and Molecular Biology, College of Sciences, Hanyang University) ;
- Hong, Sa-Weon (Dept. of Biochemistry and Molecular Biology, College of Sciences, Hanyang University) ;
- Lee, Jong-Ho (Dept. of Chemistry, Hanseo University) ;
- Park, Kyung-Bae (Hanaro Center, Korea Atomic Research Institute) ;
- Cho, Key-Seung (Dept. of Biochemistry and Molecular Biology, College of Sciences, Hanyang University)
- Received : 1997.11.21
- Published : 1998.07.31
Abstract
The 8-hydroxyisocoumarin, named Centipedin, which has a significant antibiotic activity, was separated and solubilized with organic solvents, such as diethyl ether from centipede Scolopendra subspinipes multilans L. Koch. The Centipedin was purified by silicic acid column and high S cation exchange chromatography followed by reverse-phase HPLC. It was confirmed that Centipedin has a potent antibiotic effectiveness against Gram-negative Klebsiella pneumoniae ATCC 8308. The results showed that Centipedin blocks both DNA replication and RNA transcription during the growth of this pathogen in vivo. The biosynthesis of antibiotic 8-hydroxyisocoumarin was studied in vivo by feeding