• Korean journal of food and cookery science
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• v.12 no.3
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• pp.295-299
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• 1996

Egg yolk oil was obtained from a roasting and Pressure egg yolks obtained from cage system, open barn system, respectively. Lipids in egg yolk oil were extracted with a mixture of chroform: methanol (2:1, v/v) and fractionated into neutral lipid, glycolipid and phospholipid by silicic aicd column chromatography. Lipid components of each fraction were determined by thin layer chromatography (TLC). The results were sum- marized as follows: lipid content of egg yolk from each cage system (A) and open barn system (B) was 31. 05% and 33.34%, and the lipid is made up of neutral lipid 76.60%, 71.23%, glycolipid 3.95％, 5.03% and phospholipids 19.45%, 23.74% respectively. Triglycerides (A: 59.3%, B: 56.3%) were the major components among the neutral lipids; monoglycerides, diglycerides, free sterols, and free fatty acids were the minor cop- monents. The major components of the glycolipids were digalactosyl diglycerides (A: 98.3%, B: 97.8%), the other components were cerebrosides. The major components of the phophoslipids were phosphatidyl choline plus phosphatidyl serine (A: 58.6%, B: 59.8%) the other components were lecithin plus sphingomyelin.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.12
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• pp.25-29
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• 1972

This experiment was carried out to investigate the role of Silicon accumlated in rice plant under different conditions of light and humidity, using radioisotopes Ca-45, Mn-54, and P-32. This results obtained in are as follows; 1. Light effect is more severe in phosphate uptake by rice plant than is calcium. Amounts of phosphate uptake in light condition is six times more than in dark conditions, while that of calcium is double. 2. Change of relative humidity affects calcium absorption and transport from root to shoot. It seems not to be influenced in phosphate and manganese uptake by relative humidity. 3. More uptake of each element Ca-45, P-32, or Mn-54 was found in the rice plant applied with silicic acid. It is considered that there must be some relationship between silicon content and ion uptake in rice plant. 4. The transport ratio of nutrient from root to shoot shows a specific pattern that calcium is approximately 1.0 manganese 0.5 and phosphate 0.2 respectively.

• Journal of Ginseng Research
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• v.10 no.2
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• pp.141-150
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• 1986

This study was attempted to screen the cytotoxic activity of petroleum ether ex- tract from panax ginseng root against human colon cancer cells. Two extracts of panax ginseng root, crude and partially purified, were used for this experiment. The crude extract was prepared by extraction with petroleum ether using Soxhlet aparatus for 12 to 15 hours from panax ginseng and the extract was partially purified by silicic acid column with mixture of petroleum ether: ethyl ether (70 : 30, v/v). Three species of human colon cancer cells, HRT-18, HCT-48 and HT-29, were maintained in DMEM (Dulbecco's modified Eagle medium), and the cells were cultured in DMEM containing serial concentration of the crude or partially purified fraction to observe the cytotoxic activity of the both extracts. The effects of incubation time and concentration of the both extracts in culture medium against the growth of the each cancer cell were determined. The results obtained are summarized as follows: 1. The doubling times of the HRT-18, HCT-48 and HT-29 cells were about 20, 24 and 22 hours, respectively. 2, The inhibitory effects of the crude extract on the growth of cancer cells were increased according to the rise of concentration of the extract and incubation time. 3. The inhibitory effect of partially purified fraction on the growth of HRT-18 cell was about 4 times stronger than that of the crude extract under same experimental condition. 4 The inhibitory effects of the crude and purified fraction on the growth of each cancer cell were shown difference by the kind of the cancer cell. In view of the above results, it could be said that the petroleum ether extract of panax ginseng root inhibited the division of the human colon cancer cell, in vitro.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.3
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• pp.287-299
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• 1989

This research was conducted in order to investigate thermal stability and nutritional value of corn lipids and proteins during thermal processing. The lipids of raw and popped corn were fractionated and analyzed by column and gas chromatography. The effect of thermal processing on corn proteins was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid analysis. There was no remarkable change in proximate compositions during thermal processing. The lipid fractions obtained by silicic acid column chromatography were composed of neutral lipid(93.5%), glycolipid(3.8%), and phospholipid(2.7%), Although the thermal processing showed the increase in the ratio of unsaturated/saturated fatty acid, there was no significant differences in the lipid composition between raw and popped corn. Most of each protein fractions had lower molecular weight than 25,000 dalton and albumin fractions were distributed in the molecular weight range 11,500-94,000 daltons. Popped corn proteins did not show marked differences in their electrophoretic migrations when compared with raw corn proteins.

• Journal of Ginseng Research
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• v.4 no.1
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• pp.31-39
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• 1980

This study was prepared to observe some enzyme activities in the liver of mice treated with extracts of Ginseng anticancer compound, separated from the petroleum ether extracts by silicic acid chromatography, has the cytotoxic activity against cancer cells. Swiss mice, 72 heads were used (or this experiment and they were divdied into control, test group I and test group If, that test group I was injected crude extract and test group II was injected anticancer compound, while the control group was injected 0.9% NaCl solution. The injections were carried out 1,2,4 and 8 times once a day for 1-8 day, respectively. The liver was removed carefully from the mice at 24 hours after drugs injected, and homogenized at 4$^{\circ}C$ for enzyme study. The activities of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase(GPT) were determined by Reitmen and Frankel method and lactic acid dehydrogenase activity was determined by Wroblewski methods in vitro. The results obtained are summarized as follows; 1. The GOT activity was increased 26%, crude extract and 16%, anticancer compound than those of control at 1st injected groups and decreased gradually according to increase of injection time, at 8th injected groups, the GOT activity was decresed by 16%, crude extract and 12%, anticancer compound. 2. The GPT activity was not changed significantly at 1st and 2nd injected groups, but, at 4th injected groups, the GPT activity was decreased 20%, crude extract and 14%, anticancer compound. While the GPT activity was recovered to normal value at 8th injected groups. 3. At 1st injected groups, the LDH activity was increased 17%, anticancer compound, while those of crude extract was shown normal value. At 2nd injected groups, the LDH activity increased 35yo:, crude extract while those of anticancer compound was showed normal value. And the LOH activity was recovered gradually at 4th and 8th injected groups.

• Journal of Ginseng Research
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• v.8 no.2
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• pp.153-166
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• 1984

The anticancer activities of petroleum ether extract of Panax ginseng root(crude GX) and its partially purified fraction from silicic acid column chromatography (7:3 GX) were studied with Sarcoma 180(S-180) or Walker carcinosarcoma 256 (Walker 256) in vivo and with L1210 leukemic lympocyte in vitro. Potential cytotoxic activities of the crude GX and against L1210 cells were compared with those of 5-Fluorouracil (5-FU) and saponin derivatives (Panax-diol, Panax-triol, Diol saponin, Triol saponin) in vitro. In order to observe the physiological effects of the crude GX and 7:3 GX on the animals with cancer, hemoglobin(Hb), red blood cell(R.B.C) and white blood cell after treatment with each GX in comparison with corresponding control groups, respectively. The anticancer effects of the crude GX and 7:3 GX were estimated by measuring the survival time of S-180 bearing mice after treatment with them. The experimental results obtained are summarized as follows; 1. The one unit of cytotoxic activity against L1210 cells was equivalent to 2.54$\mu\textrm{g}$ and 0.88$\mu\textrm{g}$of the crude GX and 7:3 GX per ml of culture medium, respectively. 2. The cytotoxic activities of Panax-diol, Panax=triol, Diol saponin and triol saponin against L1210 cells were not detected. 3. The anticancer activities of 5-FU against L1210, S-180 and Walker 256 were very effective in vivo and vitro tests. 4. The significantly increased W.B.C values of mice after inoculation with S-180 cells were reduced to normal range by the crude GX treatment. 5. The significantly decreased Hb values of rats after inoculation with Walker 256 were recovered to normal range by oral administration of the crude GX. 6. The survival times of mice inoculated with S-180 cells were extended about 1.5 to 2 times by the 7:3 GX treatment compared with their control group.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1453-1457
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• 1999

The optimal conditions of alkali isomerization to obtain conjugated linoleic acid (CLA) concentrate from vegetable oils which have antioxidant and anticarcinogenic properties were studied. The result of alkali isomerization of various vegetable oils indicated that CLA content of safflower oil which contains more linoleic acid than any other vegetable oils was the highest of all experimental vegetable oils. During alkali isomerization, the amount of cis-9, trans-11 CLA and total CLA content in safflower oil was the highest at $8{\sim}11%$ KOH concentration and $180{\sim}185^{\circ}C$. But heating time had no effect on CLA formation after $20{\sim}40$ minutes. As a result of alkali isomerization of neutral lipid, glycolipid and phospholipid in safflower oil, CLA content of neutral lipid class was higher than any other lipid classes. By urea treatment and HPLC fractionation, total CLA content in alkali-isomerized safflower oil increased to 95.4% from 78.9%.

• Journal of the Mineralogical Society of Korea
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• v.10 no.1
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• pp.60-73
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• 1997

The talc of the Daeheung, Pyeongan, and Cheongdang (Shinyang) talc deposits in the Yesan-Gongju-Cheongyang area is a hydrothermal alteration product of serpentinite originated from ultramafic rocks. The mineral assemblages in alteration zones are: serpentine, serpentine-talc, talc, talc-chlorite, talc-phlogopite-chlorite, and talc-tremolite-chlorite. Chemical distributions in both the Al2O3-FeO-MgO system and the immobile elements suggest that the serpentine-talc and talc rocks are the reaction product of ultramafic rocks and silicic hydrothermal solution without addition of other granitic components, whereas chlorite-, phlogopite-, and tremolite-bearing rocks are the metasomatic alteration product of serpentinite by hydrothermal solution affected by granitic gneiss. Discontinuities in the immobile element ratios of mineral assemblages are due to changes in their mineralogy. The relative contents of Al2O3, TiO2, Zr in the talc-phlogopite-chlorite and talc-tremolite-chlorite rocks increase irregularly with increasing phlogopite, tremolite, and/or chlorite contents in contrast to other ore types. But the relative contents of Cr, Ni, and Co are uniform in all the mineral assemblages. Chemistry of each mineral assemblage formed by steatitization of serpentinite suggests that Cr, Co, Ni, MgO, and Fe2O3 are relatively immobile during the alteration, whereas SiO2, Al2O3, CaO, and K2O are highly increased. The contents of chlorite, phlogopite, and tremolite in each mineral assemblage might be controlled by addition of Al2O3, K2O, and CaO, respectively. The high contents of other elements than immobile elements in the altered rocks as compared with unaltered rocks indicate that a large amount of elements were introduced from hydrothermal solution up to about 8∼41% in total mass showing maximum value in the talc-phlogopite-chlorite rock.

• Korean Journal of Agricultural Science
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• v.51 no.2
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• pp.227-238
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• 2024

Methane (CH₄) is an important greenhouse gas, with a short-term greenhouse effect 80-fold that of carbon dioxide. Blast furnace slag used as a base ingredient for silicate fertilizer, and contained Fe³⁺, which acts as reduction of CH₄ emissions in flooded rice paddy. This study was evaluated the effects of the silicate fertilizer with different rates of the iron slag on CH₄ emissions and rice growth. In this study, the SF 0.0% was applied with silicate fertilizer containing 0.0% of the iron slag, while the SF 2.5% and SF 5.0% were treated with silicate fertilizer containing 2.5 and 5.0%, respectively. The CH₄ emissions during rice cropping period were assessed using a closed-chamber method and then determined by Gas chromatography. The CH₄ fluxes were reduced by 17% (SF 0.0%), 17% (SF 2.5%), and 8% (SF 5.0%) compared to the treatment with only-inorganic fertilization (control). Conversely, rice grain yield increased by 15 - 30% compared to the control owing to the improvement of soil quality by silicate fertilization. In particular, soil pH, available phosphorus and available silicic acid content were increased with the increase in the iron slag rates from 0.0 to 5.0%. These contributed to a significant increase in rice growth such as 1,000-grains weight and percentage of filled grains. Consequently, these findings were indicated that the application of silicate fertilizer containing 2.5 - 5.0% of iron slag would be the most effective in both CH₄ reduction and rice growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.1
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• pp.1-8
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• 2017

We investigated the effect of silicate coating of rice seeds on bakanae disease incidence and the quality of seedlings raised in seedling boxes and transplanted into pots. The silicate-coated rice seed (SCS) was prepared as follows. Naturally infested rice seeds not previously subjected to any fungicidal treatment were dressed with a mixture of 25% silicic acid at pH 11 and 300-mesh zeolite powder at a ratio of 50 g dry seed - 9 mL silicic acid - 25 g zeolite powder. The following nursery conditions were provided : Early sowing, dense seeding in a glass house with mulching overnight and no artificial heating, which were the ideal conditions for determining the effect on the seed. The nursery plants were evaluated for Gibberella. fujikuroi infection or to determine the recovery to normal growth of infected nursery plants in the Wagner pot. Seedlings emerged 2-3 days earlier for the SCS than they did for the non-SCS control, while damping-off and bakanae disease incidence were remarkably reduced. Specifically, bakanae disease incidence in the SCS was limited to only 7.8% for 80 days after sowing, as compared to 91.6% of the non-SCS control. For the 45-days-old SCS nursery seedlings, the fresh weight was increased by 11% and was two times heavier, with only mild damage compared to that observed for non-SCS. Even after transplanting, SCS treatment contributed to a lower incidence of further infections and possibly to recovery of the seedlings to normal growth as compared to that observed in symptomatic plants in the pot. The active pathogenic macro-conidia and micro-conidia were considerably lower in the soil, root, and seedling sheath base of the SCS. In particular, the underdeveloped macro-conidia with straight oblong shape without intact septum were isolated in the SCS ; this phenotype is likely to be at a comparative etiological disadvantage when compared to that of typical active macro-conidia, which are slightly sickle-shaped with 3-7 intact septa. A active intact conidia with high inoculum potential were rarely observed in the tissue of the seedlings treated only in the SCS. We propose that promising result was likely achieved via inhibition of the development of intact pathogenic conidia, in concert with the aerobic, acidic conditions induced by the physiochemical characteristics associated with the air porosity of zeolite, alkalinity of silicate and the seed husk as a carbon source. In addition, the resistance of the healthy plants to pathogenic conidia was also important factor.