• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.7B
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• pp.528-535
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• 2012

This paper proposes silence drop first(SDF) active buffer management algorithm to increase the voice capacity when silence suppression is used. This algorithm finds and drops silence packet rather than voice packet in the queue for resolving buffer overflow of queue. Simulations with voice codec of G.729A and G.711 are performed. By using proposed SDF algorithm, the voice capacity is increased by 84.21% with G.729A and 38.46% with G.711. Further more, SDF algorithm reduces the required link capacity and loosens the silence packet inter-arrival time limit to provide target voice quality compared with that of conventional algorithms.

• Molecules and Cells
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• v.42 no.5
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• pp.397-405
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• 2019

The regulatory role of long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) in both cancerous and noncancerous cells have been widely reported. This study aimed to evaluate the role of lncRNA GAS5 in heart failure caused by myocardial infarction. We reported that silence of lncRNA GAS5 attenuated hypoxia-triggered cell death, as cell viability was increased and apoptosis rate was decreased. This phenomenon was coupled with the down-regulated expression of p53, Bax and cleaved caspase-3, as well as the up-regulated expression of CyclinD1, CDK4 and Bcl-2. At the meantime, the expression of four heart failure-related miR-NAs was altered when lncRNA GAS5 was silenced (miR-21 and miR-142-5p were up-regulated; miR-30b and miR-93 were down-regulated). RNA immunoprecipitation assay results showed that lncRNA GAS5 worked as a molecular sponge for miR-142-5p. More interestingly, the protective actions of lncRNA GAS5 silence on hypoxia-stimulated cells were attenuated by miR-142-5p suppression. Besides, TP53INP1 was a target gene for miR-142-5p. Silence of lncRNA GAS5 promoted the activation of PI3K/AKT and MEK/ERK signaling pathways in a miR-142-5p-dependent manner. Collectively, this study demonstrated that silence of lncRNA GAS5 protected H9c2 cells against hypoxia-induced injury possibly via sponging miR-142-5p, functionally releasing TP53INP1 mRNA transcripts that are normally targeted by miR-142-5p.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.4
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• pp.652-657
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• 2009

This paper proposes an adaptive noise suppression system based on human auditory model to enhance speech signal that is degraded by various background noises. The proposed system detects voiced, unvoiced and silence sections for each frame and implements an adaptive auditory process, then reduces the noise speech signal using a neural network including amplitude component and phase component. Based on measuring signal-to-noise ratios, experiments confirm that the proposed system is effective for speech signal that is degraded by various noises.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2011.04a
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• pp.347-350
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• 2011

Automobiles should be light and practical to reduce the price of manufacturing and maintaining expense. Hatchback vehicles are far from the silence for its practicality. This paper introduced the effective method of the planning of the asphalt material which applies the asphalt damping material. This paper showed the experiment which restrains the vibration of the vehicle trunk using asphalt damping material and chose the position of damping material using the velocity map and strain energy map and planed the optimum position.

• Horticultural Science & Technology
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• v.35 no.3
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• pp.323-332
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• 2017

Homology-specific transcriptional and post-transcriptional silencing, an intrinsic mechanism of gene regulation in most eukaryotes, can be induced by anti-sense, co-suppression, or hairpin-based double-stranded RNA. Hairpin-based RNA interference (RNAi) has been applied to analyze gene function and genetically modify crops. However, RNAi vector construction usually requires high-cost cloning steps and large amounts of time, or involves methods that are protected by intellectual property rights. We describe a more effective method for generating intron-spliced RNAi constructs. To produce intron-spliced hairpin RNA, an RNAi cassette was ligated with the first intron and splicing sequences of the Brassica rapa ssp. pekinensis histone deacetylase 1 gene. This method requires a single ligation of the PCR-amplified target gene to SpeI-NcoI and SacI-BglII enzyme sites to create a gene-specific silencing construct. We named the resulting binary vector system pKHi and verified its functionality by constructing a vector to silence DIHYDROFLAVONOL 4-REDUCTASE (DFR), transforming it into tobacco plants, and confirming DFR gene-silencing via PCR, RT-qPCR, and analysis of the accumulation of small interfering RNAs. Reduction of anthocyanin biosynthesis was also confirmed by analyzing flower color of the transgenic tobacco plants. This study demonstrates that small interfering RNAs generated through the pKHi vector system can efficiently silence target genes and could be used in developing genetically modified crops.

• The KIPS Transactions:PartC
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• v.16C no.5
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• pp.661-668
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• 2009

The Extended real-time Polling Service (ErtPS) is proposed tosupport QoS of VoIP service with silence suppression which generates variable size data packets in IEEE 802.16e systems. If the silence is suppressed, VoIP should support Comfort Noise Generation (CNG) which generates comfort noise for receiver's auditory sense to notify the status of connection to the user. CNG mode in silent-period generates a data with lower bit rate at long packet transmission intervals in comparison with talk-spurt. Therefore, if the ErtPS, which is designed to support service flows that generate data packets on a periodic basis, is applied to silent-period, resources of the uplink are used inefficiently. In this paper, we proposed the Improved ErtPS algorithm for efficient resource utilization of the silent-period in VoIP traffic supporting CNG. In the proposed algorithm, the base station allocates bandwidth depending on the status of voice at the appropriate interval by havingthe user inform the changes of voice status. The Improved ErtPS utilizes the Cannel Quality Information Channel (CQICH) which is an uplink subchannel for delivering quality information of channel to the base station on a periodic basis in 802.16e systems. We evaluated the performance of proposed algorithm using OPNET simulator. We validated that proposed algorithm improves the bandwidth utilization of the uplink and packet transmission latency

• Journal of Korea Multimedia Society
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• v.10 no.4
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• pp.450-461
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• 2007

In this paper, we propose the method to use power-saving mode (PSM) applicable to non real-time traffic and PSM applicable to real-time traffic simultaneously, for VoIP traffic with silence suppression. The proposed method uses PSC II during talk-spurt interval of parties A and/or B and uses PSC I or probabilistic sleep interval decision (PSID) method during mutual silence interval, respectively. To evaluate the performance of hybrid PSM (HPSM) based on PSC II or PSID method, we present average buffering delay, energy consumption of mobile station and VoIP packet drop probability with simulation runs. Results shows that proposed HPSM decreases energy consumption of mobile station up to 25 % while satisfying the packet drop probability within QoS requirement in case of end-to-end VoIP connection.

• Parasites, Hosts and Diseases
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• v.46 no.1
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• pp.1-15
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• 2008

Introduction of double-stranded RNA (dsRNA) into some cells or organisms results in degradation of its homologous mRNA, a process called RNA interference (RNAi). The dsRNAs are processed into short interfering RNAs (siRNAs) that subsequently bind to the RNA-induced silencing complex (RISC), causing degradation of target mRNAs. Because of this sequence-specific ability to silence target genes, RNAi has been extensively used to study gene functions and has the potential to control disease pathogens or vectors. With this promise of RNAi to control pathogens and vectors, this paper reviews the current status of RNAi in protozoans, animal parasitic helminths and disease-transmitting vectors, such as insects. Many pathogens and vectors cause severe parasitic diseases in tropical regions and it is difficult to control once the host has been invaded. Intracellularly, RNAi can be highly effective in impeding parasitic development and proliferation within the host. To fully realize its potential as a means to control tropical diseases, appropriate delivery methods for RNAi should be developed, and possible off-target effects should be minimized for specific gene suppression. RNAi can also be utilized to reduce vector competence to interfere with disease transmission, as genes critical for pathogenesis of tropical diseases are knockdowned via RNAi.

• Tuberculosis and Respiratory Diseases
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• v.49 no.3
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• pp.298-309
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• 2000

Background : The antitumor effects of herpes simplex virus thymidine kinase (HSV-tk) and ganciclovir (GCV) strategies for cancer gene therapy have a the following advantages : 1) a direct cytotoxicity to HSV-tk modified cancer cells by GCV 2) a cell death by the local transfer of toxic metabolites from the HSV-tk modified cells to nearby unmodified tumor cells (bystander effect), and 3) in vivo bystander effect such as antitumor-immunity. Retroviral and adenoviral sequences can silence transgene expression in cells and mice. In this study, we investigated the above described advantages of HSV-tk/GCV strategy in Lewis lung cell and mouse lung cancer model using retroviral vector and adenoviral vector. Also, we observed whether the expression of a silenced gene can be reactivated by treating cells with butyrate. Methods : Retrovirus-HSV-tk and adenovirus-HSV-tk vectors were used for the transduction of Lewis lung carcinoma (LLC) cells. The change of HSV-tk expression by butyrate was measured by Western blol The antitumor activities containing bystander effect were observed in vivo (by MTT assay) and in vivo tumor models of various combinations of LLC and LLC-tk. Results : 1. Butyrate induced the enhancement of HSV-tk expression from adenovirally transduced cells but not from retrovirally transduced cells. 2. Both retrovirus-HSV-tk and adenovirus-HSV-tk vectors with GCV treatment were effective for killing of tumor cell in vitro and suppression of LLC tumorigenicity. Bystander effect was responsible for killing of mixture of LLC-tk and LLC in vitro and in vivo-tumorigenicity model. Conclusion : Butyrate could augment adenovirus-mediated HSV -tk gene expression. Cancer gene therapy with HSV-tk suicide gene by retroviral and adenoviral vector seems to be an effective approach for lung cancer therapy.