• Fisheries and Aquatic Sciences
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• 제25권10호
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• pp.499-516
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• 2022

Endobiotic microalgae inhabit various groups of organisms, including bivalves. In this group, the association between the giant molluscs Tridacna and Symbiodinium is one of the most recognizable. This consortium allows hydrobionts to survive in oligotrophic waters by regulating their metabolism. The available research has provided an understanding of the interaction and adaptation of these symbionts, but the problem of the beginning of the formation of these relationships remains unresolved. In the case of Tridacninae, symbiosis is essential for the survival of bivalves, in contrast to representatives of the Mytilidae and the Coccomyxa found in them. A few works devoted mainly to the morphological aspects of invasion have shown that endobiont causes inflammation and pathology. Having data to clarify the exact "diagnosis" of the interaction of these organisms is not enough. It is possible that the relationship between bivalves and Coccomyxa is in the early stages of being established, which may lead to mutualism or parasitism in the future. We assume that the analysis of works on the symbiosis of Symbiodinium and bivalves will facilitate the course of research for the less studied Coccomyxa and their hosts. By postulating the Coccomyxa represent a unique evolutionary model for the formation of a symbiotic system, it is possible to use this system to study the interaction of organisms during their initial contact. The identified signalling pathways and mechanisms that allow the photobionts to evade host immunity can be useful for constructing new forms of symbiosystems.

• 한국철도학회논문집
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• 제19권2호
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• pp.145-158
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• 2016

철도 신호 시스템의 안전 확보 기준으로 사용되는 안전무결성수준(SIL, Safety Integrity Level) 할당에 사용되는 기존 정성적 평가방법인 리스크 그래프에 대하여 소개하고, 정성적 평가의 문제점인 입력 변수의 모호성 및 안전무결성수준간 경계성 문제에 대하여 퍼지 이론 적용을 통해 문제점을 보완하는 것을 목적으로 한다. 본 모델의 퍼지 입력변수는 4가지인 심각도, 노출도, 회피도, 요구율로 구성되며, 퍼지추론(Fuzzy Inference)은 IEC 61511의 계량적 리스크 그래프를 적용하여 48개의 퍼지 규칙을 생성한다. 퍼지추론은 최대 최소 합성(Max-Min Composition)의 퍼지관계 합성연산을 적용한다. 추론 모델을 통해 도출된 최종적인 추론 결과는 퍼지 값이므로 실제 상황에 적용 가능하도록 다시 실수 값으로 변환하는 역 퍼지화 과정을 통해 최종 출력값인 안전무결성수준과 그에 해당하는 허용 해저드율을 생성하여, 최종적인 해당 해저드에 대한 안전성 요구사항을 도출한다. 마지막으로 본 평가모델 검증을 위해 CENELEC SC 9XA WG A10 보고서에 소개된 단선구간에서의 신호시스템을 대상으로 한 안전성 평가 결과와 비교한다.

• 노동경제논집
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• 제38권4호
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• pp.83-107
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• 2015

FA제도는 일정 기간 동안 한 구단에서 뛴 선수에게 타 구단과의 자유로운 연봉 협상을 통해 팀을 옮길 수 있는 기회를 제공하는 제도이다. FA을 통한 천문학적인 연봉계약은 때로 금액에 비해 성과가 현저하게 떨어지는 FA 먹튀 논쟁이 불러일으키기도 한다. 본 연구는 양충열 왕규호(2013)의 모형에 불완비 정보를 추가하여 신호모형을 이용해 FA 먹튀의 존재 가능성을 분석하였다. 분리균형(separating equilibrium)에서는 타 구단이 선수의 생산성을 완벽하게 추론할 수 있어, 먹튀는 발생하지 않는다. 따라서 먹튀는 합동균형(pooling equilibrium)에서만 발생한다. 본 연구는 먹튀가 발생하지 않는 분리균형은 존재하지 않음을 보였다. 또한 적절한 조건 하에서, 특히 강한 보상제도하에서 유일한 합동균형이 존재함을 보였다.

• The Korean Journal of Physiology and Pharmacology
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• 제12권5호
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• pp.267-274
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• 2008

Since first discovered in chick skeletal muscles, stretch-activated channels (SACs) have been proposed as a probable mechano-transducer of the mechanical stimulus at the cellular level. Channel properties have been studied in both the single-channel and the whole-cell level. There is growing evidence to indicate that major stretch-induced changes in electrical activity are mediated by activation of these channels. We aimed to investigate the mechanism of stretch-induced automaticity by exploiting a recent mathematical model of rat atrial myocytes which had been established to reproduce cellular activities such as the action potential, $Ca^{2+}$ transients, and contractile force. The incorporation of SACs into the mathematical model, based on experimental results, successfully reproduced the repetitive firing of spontaneous action potentials by stretch. The induced automaticity was composed of two phases. The early phase was driven by increased background conductance of voltage-gated $Na^+$ channel, whereas the later phase was driven by the reverse-mode operation of $Na^+/Ca^{2+}$ exchange current secondary to the accumulation of $Na^+$ and $Ca^{2+}$ through SACs. These results of simulation successfully demonstrate how the SACs can induce automaticity in a single atrial myocyte which may act as a focus to initiate and maintain atrial fibrillation in concert with other arrhythmogenic changes in the heart.

• The Korean Journal of Physiology and Pharmacology
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• 제25권6호
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• pp.555-564
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• 2021

We investigated the effects of naringenin and morin on IL-5 and ROS production in PMA+ionomycin-treated EL-4 cells with the corroboration of their antioxidant and anti-inflammatory properties using an asthma-induced mouse model. The EL-4 cell line was used to study the outcomes of naringenin or morin, followed by cell viability studies. Western blot analysis and ELISA test were used to determine Th2 mediated cytokines. In vivo studies were carried out on BALB/c mice to induce allergic asthma using ovalbumin administered intraperitoneally. Intracellular ROS was determined using 2',7'-dichlorodihydrofluorescein diacetate, followed by serum enzymatic (AST and ALT) estimations and inflammatory cell count in the bronchoalveolar lavage fluid (BALF) and lung tissues. Histopathological studies were conducted to examine lung tissue-stained architecture. Our findings suggested that naringenin and morin significantly suppressed IL-5 and ROS production via various pathways. Interestingly, by reducing NFAT activity, naringenin and morin stimulated HO-1 expression, thereby suppressing IL-5 secretion due to regulating the transcription factor Nrf2 via P13/Akt or ERK/JNK signalling pathways in EL-4 cells, demonstrating the involvement of HO-1 expression in inhibiting asthmatic inflammation. The increased inflammatory cells in the BALF were substantially decreased by both naringenin and morin, followed by inhibition in the elevated Th-2 cytokines levels. The TNF-α protein levels in an allergic asthma mouse model were significantly reduced by suppressing Akt phosphorylation and eosinophil formation. Recent findings confirmed that naringenin and morin possess the potential to control asthma-related immune responses through antioxidant and anti-inflammatory properties, indicating potential therapeutic agents or functional foods.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.827-837
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• 2019

The present study was conducted with the aim to investigate the ameliorative effects of a new soybean product (cheonggukjang) fermented with Bacillus amyloliquefaciens SCGB1 (SFBA) in atopic dermatitis (AD) mouse model. Visual evaluation of AD induction in the mice indicated the remarkable control of SFBA in reducing the pathological severity of AD-like skin lesions reported as the SCORAD score of AD clinical symptoms. The results revealed that SFBA reduced dorsal skin and epidermal thickness to a similar extent with prednisolone. Further analysis revealed the dominance of SFBA in restraining mast cell infiltration in the dermis; immunoglobulin-E expression in serum; and TH2 IL-4 cytokine and itch-related IL-31 cytokine in the mice skin and serum. SFBA also suppressed scratching behaviours in mice induced by compound 48/80. Further histological findings also revealed the alleviation of collagen fiber deposition in dermal skin of the AD mice model. These actions of SFBA were examined to be mediated by its suppression of the phosphorylation activation of key signalling molecules such as $NF-{\kappa}B$ and MAPK responsible for the induction of cytokine production. Thus, SFBA can be considered as a promising functional food for managing clinical, histological and immunological spectra associated with AD.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.24-25
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• 2003

The zebrafish (Danio rerio) is now the pre-eminent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. I will talk about positional cloning of two developmental mutants in zebrafish. The first mutant is headless: The vertebrate organizer can induce a complete body axis when transplanted to the ventral side of a host embryo by virtue of its distinct head and trunk inducing properties. Wingless/Wntantagonists secreted by the organizer have been identified as head inducers. Their ectopic expression can promote head formation, whereas ectopic activation of Wnt signalling during early gastrulation blocks head formation. These observations suggest that the ability of head inducers to inhibit Wntsignalling during formation of anterior structures is what distinguishes them from trunk inducers that permit the operation of posteriorizing Wnt signals. I describe the zebrafish headless (hdl) mutant and show that its severe head defects are due to a mutation in T-cell factor-3 (Tcf3), a member of the Tcf/Lef family. Loss of Tcf3 function in the hdl mutant reveals that hdl represses Wnt target genes. I provide genetic evidence that a component of the Wntsignalling pathway is essential in vertebrate head formation and patterning. Second mutant is mind bomb: Lateral inhibition, mediated by Notch signaling, leads to the selection of cells that are permitted to become neurons within domains defined by proneuralgene expression. Reduced lateral inhibition in zebrafish mib mutant embryos permits too many neural progenitors to differentiate as neurons. Positional cloning of mib revealed that it is a gene in the Notch pathway that encodes a RING ubiquitin ligase. Mib interacts with the intracellular domain of Delta to promote its ubiquitylation and internalization. Cell transplantation studies suggest that mib function is essential in the signaling cell for efficient activation of Notch in neighboring cells. (중략)

• 대한의생명과학회지
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• 제15권4호
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• pp.319-326
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• 2009

Extracellular ATP elicits diverse physiological effects by binding to the G-protein-coupled P2Y receptors on the plasma membrane. In addition to the short-term effects of extracellular nucleotides on cell functions, there is evidence that such purinergic signalling can have long-term effects on cell proliferation, differentiation and death. The 3T3-L1 cell line derived from mouse embryo is a well-established and commonly utilized in vitro model for adipocytes differentiation and function. However, the distributions and roles of P2Y subtypes are still unknown in the preadipocyte. In this study, we identified the distributions and roles of P2Y subtypes in preadipocyte using $Ca^{2+}$ imaging and realtime PCR. ATP increased the $[Ca^{2+}]_i$ in a concentration-dependent manner. ATP increased $Ca^{2+}$ in absence and/or presence of extracellular $Ca^{2+}$. Suramin, non-selective P2Y blocker, largely blocked the ATP-induced $Ca^{2+}$ response. U73122, a PLC inhibitor, completely inhibited $Ca^{2+}$ mobilization in 3T3-L1 cells. The mRNA expression by realtime PCR of P2Y subtypes was $P2Y_2:P2Y_5:P2Y_6=1.0:12.5:0.3$. In conclusion, we showed that $P2Y_5$ receptor is a dominant purinergic receptor in preadipocytes, and multiple P2Y receptors could involve in differentiation and migration via regulating of intracellular calcium concentration.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7617-7623
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• 2014

Objective: The mechanism of action of fatty acid synthase (FASN) in drug tolerance of breast cancer cells with epithelial-mesenchymal transition (EMT) features was investigated. Methods: The breast cancer cell line MCF-7-MEK5 with stably occurring EMT and tumour necrosis factor-${\alpha}$ (TNF-${\alpha}$) tolerance was used as the experimental model, whereas MCF-7 acted as the control. Tumour cells were implanted into nude mice for in vivo analysis, and cerulenin was used as a FASN inhibitor. RT-PCR, real-time quantitative PCR and Western blot were employed to detect the expression of FASN, TNFR-1, TNFR-2, Wnt-1, ${\beta}$-catenin and cytC at the RNA and protein levels. Results: Compared with MCF-7, TNFR-1 expression in MCF-7-MEK5 was slightly changed, TNFR-2 was decreased, and FASN, Wnt-1, ${\beta}$-catenin and cytC were increased. The expression of Wnt-1 and ${\beta}$-catenin in MCF-7-MEK5 decreased after cerulenin treatment, whereas cytC expression increased. Conclusions: The important function of FASN in the drug tolerance of breast cancer may be due to the following mechanisms: FASN downregulated TNFR-2 expression through lipid rafts to make the cells less sensitive to TNF-${\alpha}$, and simultaneously activated the Wnt-$1/{\beta}$-catenin signalling pathway. Thus, cytC expression increased, which provided cells with anti-apoptotic capacity and induced drug tolerance.

• 한국통신학회논문지
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• 제35권11A호
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• pp.1051-1058
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• 2010

여러 종류의 접속망이 혼재할 것으로 예상되는 차세대 무선 네트워크에서 매크로 이동성을 지원할 수 있는 기법으로 mobile IP (MIP)와 SIP가 주목 받고 있다. 그 특성상 MIP는 TCP를 사용하는 비실시간 서비스에, SIP는 RTP/UDP를 사용하는 실시간 서비스에 적합하며, 따라서 두 종류의 서비스를 모두 사용하는 단말의 경우 MIP와 SIP를 함께 사용하여야 효율적인 이동성 관리가 가능하다. 이를 위해서 지금까지 여러 종류의 MIP-SIP 연동 기법들이 제안되었지만 아직은 두 프로토콜의 수행 과정에 초점이 맞춰져 있어서 뚜렷한 성능 향상을 보이지 못하고 있다. 본 논문에서는 보다 발전된 MIP인 fast MIPv6와 early SIP mobility를 이용하여 MIP와 SIP를 효율적으로 연동할 수 있는 방안을 제시한다. 해당 기법은 MIP와 SIP mobility를 동시에 수행함으로써 서비스의 중단 시간을 줄여 QoS를 보장한다. 또한 본 논문에서는 제시한 기법의 성능 분석을 위해서 지연 모델을 정의하고 그 성능을 보인다.