• International Journal of Stem Cells
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• 제16권3호
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• pp.293-303
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• 2023

Background and Objectives: The physiological oxygen tension in fetal brains (~3%, physioxia) is beneficial for the maintenance of neural stem cells (NSCs). Sensitivity to oxygen varies between NSCs from different fetal brain regions, with midbrain NSCs showing selective susceptibility. Data on Hif-1𝛼/Notch regulatory interactions as well as our observations that Hif-1𝛼 and oxygen affect midbrain NSCs survival and proliferation prompted our investigations on involvement of Notch signalling in physioxia-dependent midbrain NSCs performance. Methods and Results: Here we found that physioxia (3% O₂) compared to normoxia (21% O₂) increased proliferation, maintained stemness by suppression of spontaneous differentiation and supported cell cycle progression. Microarray and qRT-PCR analyses identified significant changes of Notch related genes in midbrain NSCs after long-term (13 days), but not after short-term physioxia (48 hours). Consistently, inhibition of Notch signalling with DAPT increased, but its stimulation with Dll4 decreased spontaneous differentiation into neurons solely under normoxic but not under physioxic conditions. Conclusions: Notch signalling does not influence the fate decision of midbrain NSCs cultured in vitro in physioxia, where other factors like Hif-1𝛼 might be involved. Our findings on how physioxia effects in midbrain NSCs are transduced by alternative signalling might, at least in part, explain their selective susceptibility to oxygen.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2739-2744
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• 2012

Background: Renal-cell carcinoma (RCC) is resistant to almost all chemotherapeutics and radiation therapy. ${\beta}$-Elemene, a promising anticancer drug extracted from a traditional Chinese medicine, has been shown to be effective against various tumors. In the present study, anti-tumor effects on RCC cells and the involved mechanisms were investigated. Methods: Human RCC 786-0 cells were treated with different concentrations of ${\beta}$-elemene, and cell viability and apoptosis were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry, respectively. Protein expression was assayed by western blotting. Autophagy was evaluated by transmission electron microscopy. Results: ${\beta}$-Elemene inhibited the viability of 786-0 cells in a dose- and time-dependent manner. The anti-tumor effect was associated with induction of apoptosis. Further study showed that ${\beta}$-elemene inhibited the MAPK/ERK as well as PI3K/Akt/mTOR signalling pathways. Moreover, robust autophagy was observed in cells treated with ${\beta}$-elemene. Combined treatment of ${\beta}$-elemene with autophagy inhibitors 3-methyladenine or chlorochine significantly enhanced the anti-tumor effects. Conclusions: Our data provide first evidence that ${\beta}$-elemene can inhibit the proliferation of RCC 786-0 cells by inducing apoptosis as well as protective autophagy. The anti-tumor effect was associated with the inhibition of MAPK/ERK and PI3K/Akt/mTOR signalling pathway. Inhibition of autophagy might be a useful way to enhance the anti-tumor effect of ${\beta}$-elemene on 786-0 cells.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.232-238
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• 2011

Hazard analysis provides the basic foundation for system safety. Hazard analysis is performed to identify hazards, hazard effects, and hazard causal factors. Hazard analysis is used to determine system risk, to determine the significance of hazards, and to establish design measures that will eliminate or mitigate the identified hazards. Hazard analysis is used to systematically examine systems, subsystems, facilities, components, software, personnel, and their interrelationships, with consideration given to logistics, training, maintenance, test, modification, and operational environments. This paper present hazard analysis techniques which is commonly used in railway signalling, comparised their benefits and limitations.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.11-32
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• 2009

Objective : This study was designed to investigate anti-cancer and whitening activities (LC). So it was investigated the effects of LC on proliferation rates of melanoma genetic profile by LC. Methods : The genetic profile for the effect of LC on human derived melanoma cell, SK-MEL-2, was measured using microarray technique, and the functional analysis on these genes were conducted. Total 441 genes were up-regulated and 830 genes down-regulated in cells treated with LC. Genes induced or suppressed by LC were all mainly concerned with basic signalling pathways, which are involved in cell growth, differentiation and migration. Especially, many genes, which are related in apoptosis and cell cycle arrest were up-regulated by treatment with LC, and genes related in cell cycle were down-regulated. Result : The network of total protein interactions were identified by using cytoscape program, and some key molecules, such as BCL2L1, SIN3A, SMAD2 and c-myc that can be used for elucidation of therapeutical mechanism of medicine in the future. Conclusion : These results suggest possibility of LC as addition drug and whitening cosmetics. In addition, it was also suggested that related mechanisms are involved in BCL2L1, SIN3A, SMAD2 and c-myc related signalling pathways.

• 노동경제논집
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• 제34권2호
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• pp.79-107
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• 2011

본 연구는 우리나라의 청년노동시장에서 영어점수가 (1) 선별효과, (2) 신호효과 및 (3) 임금효과에 미치는 영향에 관하여 분석했다. 분석 결과는 다음과 같다. 첫째, 영어점수는 선별 신호과정에서 선별도구 및 신호기재로 이용되고 있는 것으로 나타났다. 둘째, 일정 수준 이하의 영어점수를 보유한 구직자는 선별 신호과정에서 불이익을 받는 것으로 나타났다. 마지막으로 능력편의를 통제한 도구변수법을 이용하여도 영어점수의 임금프리미엄이 존재하는 것으로 나타났다.

• 대한한방부인과학회지
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• 제23권2호
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• pp.71-84
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• 2010

Purpose: In the theory of traditional medicine, Glenditsia spina(GS) can resolve carbuncle, relive swelling, dispel wind and destroy parasites. This study was designed to investigate the effects of GS on gene expression of ovarian tissue in polycystic ovary syndrome(PCOS) rats. Methods: In this experiment, female rats injected with a single dose of 2 mg estradiol valerate(EV) and GS was given for 5 weeks. The genetic profile for the effects on ovarian tissue in PCOS rats was measured using microarray technique, and the functional analysis on these genes was conducted. Results: 985 genes were increased in control and restored to normal level in GS group. (B), 733 genes were decreased in control group and restored to normal level in GS group. (F). Metabolic pathways related in B group genes were Graft-versus-host disease, Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Small cell lung cancer, Type I diabetes mellitus. Metabolic pathways related in F group genes were Antigen processing and present, Adipocytokine signalling pathway, Focal adhesion, ECM-receptor interaction, Pancreatic cancer, Notch signalling pathway, Tight junction. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as c-Fos, c-Myc, ABL1 related in B group, MAPK8, RASA1, CALR related in F group that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conclusion: These results suggest possibility of GS as anti-cancer and anti-hyperplasia drug in PCOS. In addition, the present author also suggests that related mechanisms are involved in suppression of proto-oncogene such as c-Fos, c-Myc and ABL1, and in regulation of cell cycle such as RASA1.

• 한국산학기술학회논문지
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• 제15권4호
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• pp.2355-2362
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• 2014

전기철도 선로변에 포설되어 있는 통신선에는 유도전압이 발생하여, 통신품질 저하와 기기 오작동 및 안전문제를 유발할 수 있다. 이와 같은 유도전압은 전차선과 통신선의 자계결합에 의해 유발되며, 구조물이나 레일 등의 금속물이 근처에 있을 경우 차폐영향을 받는다. 본 논문에서는 통신선 주변에 금속관이 매설되어 있을 경우에 발생하는 차폐효과를 실험을 통해 정량적으로 분석하였다. 고속철도 현장에서의 실험 결과, 금속관이 통신선으로부터 1~2m 거리에 병행매설되어 있을 경우, 금속관의 차폐효과는 20% 정도인 것으로 측정되었다. 또한, 이격거리가 4m 이상인 경우에는 차폐효과가 1% 이내로 미미하다는 것을 확인하였다. 이상의 결과는, 수도관이나 가스관, 구조물의 철근과 같은 금속 설치물의 차폐효과를 분석하는 데 활용할 수 있다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.1464-1465
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• 2007

The purpose of this paper is to identify the requirements for Surge Protective Devices (SPDs) used in protecting telecommunication and signalling system. All of these systems may be exposed to the effects of lightning and power line faults, either through direct contact or induction. These effects may subject the system to overvoltages or overcurrents or both, whose levels are sufficiently high to harm the system. SPDs are intended to provide protection against overvoltages and overcurrents caused by lightning and power line faults. This paper describes tests and requirements which establish methods for testing SPDs and determining their performance.

• 재무관리연구
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• 제24권4호
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• pp.201-232
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• 2007

본 논문은 2000$\sim$2007년 5월까지 주식분할공시와 절차를 끝낸 122개사의 131개의 주식분할자료를 바탕으로 우리나라 주식시장에서 주식분할의 공시가 정보내용을 지니고 있는가를 살펴봄으로써 우리나라 유가증권시장에서의 신호효과에 대한 실증분석과 주식분할에 따른 유동성 효과에 대하여 분석한다. 본 연구는 주식분할이 수익률에 미치는 영향과 기업들이 주식분할을 실시하는 이유를 한국의 주식시장 자료를 이용하여 분석하였다. 본 논문의 실증결과를 분석하면 다음과 같다. 첫째, 우리나라 주식시장에서 신호효과에 따른 주식분할 공시일 전후에 초과수익률이 발생하는지 여부를 신주상장일 전후의 초과수익률 발생유무와 최대주주의 주식보유비율과 비교하여 검증한 결과 주식분할 공시일과 신주상장일에 양(+)의 초과수익률이 발생하는 것으로 나타났다. 또한 이러한 초과수익률은 분할 비율이 높을수록 초과수익률이 더 크게 나타나는 것으로 확인되었다. 이러한 실증분석 결과, 우리나라 주식시장에서도 일시적으로 신호가설이 성립하는 것으로 볼 수 있다. 둘째, 우리나라 주식시장에서 주식분할의 목적에 부합되는 유동성 증대의 결과는 분할비율이 클수록 거래량의 증가가 지속적으로 증가하는 것으로 나타났다. 또한 이러한 주식분할비율에 근거한 거래량의 증가는 분할비율이 작을수록 비교적 그 비율대로 증가하는 것으로 나타났으며, 장기적인 관점에서는 분할비율이 높을수록 거래량의 증가가 나타나는 것으로 확인되었다. 셋째, 주식분할에 따른 초과수익률은 공시일과 분할일에 발생하나 그 효과가 일시적으로 나타나는 것으로 확인되었으며, 유동성 증대효과는 단기적으로는 유효하나 점차 거래량이 시장거래량에 근접해가는 추세를 확인할 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제6권3호
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• pp.143-147
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• 2002

Granule cells in dentate gyrus of hippocampus relay information from entorhinal cortex via perforant fiber to pyramidal cells in CA3 region. Their electrical activities are known to be closely associated with seizure activity as well as memory acquisition. Since action potential is a stereotypic phenomena which is based on all-or-none principle of $Na^+$ current, the neuronal firing pattern is mostly dependent on afterpotentials which follows the stereotypic $Na^+$ spike. Granule cells in dentate gyrus show afterdepolarization (ADP), while interneurons in dentate gyrus have afterhyperpolarizaton. In the present study, we investigated the ionic mechanism of afterdepolarization in hippocampal dentate granule cell. Action potential of dentate granule cells showed afterdepolarization, which was characterized by a sharp notch followed by a depolarizing hump starting at about $-49.04{\pm}1.69\;mV\;(n=43,\;mean{\pm}SD)$ and lasting $3{\sim}7$ ms. Increase of extracellular $Ca^{2+}$ from 2 mM to 10 mM significantly enhanced the ADP both in amplitude and in duration. A $K^+$ channel blocker, 4-aminopyridine (4-AP, 2 mM), enhanced the ADP and often induced burst firings. These effects of 10 mM $Ca^{2+}$ and 4-AP were additive. On the contrary, the ADP was significantly suppressed by removal of external $Ca^{2+},$ even in the presence of 4-AP (2 mM). A $Na^+$ channel blocker, TTX (100 nM), did not affect the ADP. From these results, it is concluded that the extracellular $Ca^{2+}$ influx contributes to the generation of ADP in granule cells.