• 한국방재학회 논문집
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• 제8권4호
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• pp.101-109
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• 2008

자동우량경보시설은 산간계곡 집중호우시 상류지역의 강우상황을 관측, 하류지역 행락야영객에게 자동으로 경보를 발령하거나 안내방송을 실시하여 인명피해를 예방하기 위한 시설이다. 그러나 이 시설의 기존 발령 기준은 유역특성을 고려하지 않고 일률적으로 관측강우량의 10분간의 이동합이 4 mm, 6 mm, 8 mm 이상인 경우에 각각 경계경보, 대피 1경보, 대피 2경보를 발령하였다. 이에 2003년 지방자치단체 및 유관 단체에서는 유역특성을 고려하여 위험수위, 한계유출량, 기준강우량 등의 분석을 통하여 경보발령기준을 재설정하였다. 본 연구에서는 경보발령기준이 재설정된 지역중 실적 강우 취득이 가능한 경남 거창군 월성지구를 대상으로 기존 및 재설정된 발령기준을 위험수심, 한계유출량, 기준강우량 등을 고려하여 검토하였다. 또한, 기존의 발령기준 우량, 개선된 발령기준 우량 및 금회분석을 통해 산정된 발령기준 우량을 상호비교하였다. 1분단위 변환 실적강우로 발령기준을 검토한 결과 기존안은 너무 많은 경보를 발령하게 된다. 개선안도 경보의 발령횟수 측면에서는 기준을 약간 상향조정할 필요가 있다. 그러나, 경보기준우량의 상향조정은 안전측면에서 신중할 필요가 있는 것으로 판단된다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.13-34
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• 2003

Fructose-1, 6-diphosphate (FOP), a glycolytic metabolite is reported to ameliorate inflammation and inhibit the nitric oxide production in murine macrophages stimulated with endotoxin. It is also reported that FOP has cytoprotective effects against hypoxia or ischemia/reperfusion injury in brain and heart. In this study, we examined whether FDP has protective effects on UV-induced oxidative damage in skin cell culture system and human skin in vivo. FDP had a protective role in UVB-induced LDH release and ROS accumulation in HaCaT although it did not show direct radical scavenging effect in the experiment using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). FDP also preserved cellular GSH content after UV irradiation in HaCaT and normal human fibroblast culture system. Cellular oxidative stress induces multiple downstream signaling pathways that regulate expression of multiple gene including MMP-1 and collagen, we examined the effects of FDP on UV-induced alteration of these protein expression in fibroblast culture and human skin in vivo. The increased MMP-1 expression in fibroblast and human skin by UV irradiation was significantly decreased by FDP. FDP also prevented the UV-induced decrease of collagen expression in fibroblast and human skin. Moreover, the decreasing the intracellular levels of reducing equivalents in human fibroblast by glutathione (GSH) depletion lowered the UVA dose threshold for reduction of procollagen expression, indicating that the differences of glutathione contents define the susceptibility of fibroblasts towards UV-induced reduction of procollagen expression. FDP also preserved cellular GSH content after UV irradiation, indicating that FDP has protective effects on UV-induced reduction of procollagen expression, which are possibly through maintaining intracellular reducing equivalent. Based on these premises, we examined the effect of daily use of a moisturizer containing FDP on facial wrinkle in comparison with vehicle moisturizer lacking FDP. In the clinical study, FDP significantly decreased facial wrinkle compared with vehicle alone after 6 months of use. Our results suggest that FDP has anti-aging effects in skin by increasing cellular antioxidant system and preventing oxidative signal and inflammatory reaction. Therefore FDP may be useful anti-aging agent for cosmetic purpose.

• 생명과학회지
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• 제24권7호
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• pp.713-720
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• 2014

본 연구에서는 Ardisia arborescens 에탄올 추출물(AAEE)의 항산화능과 항염증 생리활성을 in vitro assay system 및 cell culture model system을 이용하여 분석하였다. 먼저 AAEE의 항산화능을 DPPH radical 소거능으로 분석한 결과 양성 대조군으로 사용한 ascorbic acid와 유사한 정도의 높은 활성을 보여 매우 강한 항산화능을 보유함을 확인하였다. 또한 RAW 264.7 세포주를 이용한 $H_2O_2$ 및 LPS의 유도에 의해 생성된 ROS에 대한 소거능을 분석한 결과에서도 농도의존적인 강한 소거능을 보였다. 뿐만 아니라 대표적인 항산화효소로 천연물에 의한 항산화능 활성에 의해 발현이 유도되는 HO-1, TrxR1 및 그 전사 인자인 Nrf2의 단백질 발현이 AAEE의 처리에 의해 농도의존적으로 증가됨을 보였으며 이러한 HO-1, TrxR1 및 Nrf2의 발현 변화는 상위신호전달계인 MAPKs에 의해 조절될 가능성을 보였다. 한편 AAEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 세포독성 없이 농도의존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이와 같은 AAEE의 NO 생성 억제 효과는 염증 상위신호전달계인 NF-${\kappa}B$ 및 AP-1의 조절을 통해 일어날 가능성을 보였다. 이러한 결과를 통해 AAEE의 높은 항산화능과 항염증 활성을 처음으로 확인하였으며 향후 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다.

• Journal of Periodontal and Implant Science
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• 제32권4호
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• pp.733-744
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• 2002

The fibroblasts are the principal cells in the periodontal ligament of peridontium. As the periodontal ligament fibroblasts (PDLF) show similar phenotype with osteoblasts, the PDLF are thought to play an important role in alveolar bone remodeling. Cell-to-cell contacted signaling is crucial for osteoclast formation. Recently it has been reported that PDLJ enhance the bone resorbing activity of osteoclasts differentiated from hematopoietic preosteoclasts. The aims of this study were to $clarify\;^{1)}$ the mechanism of PDLF-induced osteoclastogenesis $and\;^{2)}$ whether we can use preosteoclast cell line instead of primary hematopoietic preosteoclast cells for studying the mechanism of PDLF-induced osteoclastogenesis. Osteoclastic differentiation of mouse macrophage cell line RAW264.7 was compared with that of mouse bone marrow-derived M-CSF dependent cell (MDBM), a well-known hematopoietic preosteoclast model, by examining, 1) osteoclast-specific gene expression such as calcitonin receptor, M-CSF receptor (c-fms), cathepsin K, receptoractivator nuclear factor kappa B (RANK) ,2) generation of TRAP(+) multinucleated cells (MNCs), and 3) generation of resorption pit on the $OAAS^{TM}$ plate. RAW264.7 cultured in the medium containing of soluble osteoclast differentiation Factor (sODF) showed similar phenotype with MDBM-derived osteoclasts, those are mRNA expression pattern of osteoclast-specific genes, TRAP(+) MNCs generation, and bone resorbing abivity. Formation of resorption pits by osteoclastic MNCs differentiated from sODF-treated RAW264.7, was completely blocked by the addition of osteoprotegerin (OPG), a soluble decoy receptor for ODF, to the sODF-containing culture me야um. The effects of PDLF on differentiation of RAW264.7 into　the TRAP(+) multinucleated osteoclast-like cells were examined using coculture system. PDLF were fxed with paraformaldehyde, followed by coculture with RAW264.7, which induced formation of TRAP(+) MNCs in the absence of additional treatment of sODF. When compared with untreated and fixed PDLF (fPDLF), IL-1 ${\beta}$-treated, or lipopolysaccha-ride-treated and then fixed PDLF showed two-folld increase in the supporting activity of osteoclastogenesis from RAW264.7 coculture system. There were no TRAP(+) MNCs formation in coculture system of RAW264.7 with PDLF of no fixation. These findigs suggested that we can replace the primary hematopoietic preosteoclasts for RAW264. 7 cell line for studying the mechanism of PDLF-induced osteoclastogenesis, and we hypothesize that PDLF control osteoclastogenesis through ODF expression which might be enhanced by inflammatory signals.

• Molecules and Cells
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• 제43권3호
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• pp.228-235
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• 2020

The Drosophila transmembrane semaphorin Sema-1a mediates forward and reverse signaling that plays an essential role in motor and central nervous system (CNS) axon pathfinding during embryonic neural development. Previous immunohistochemical analysis revealed that Sema-1a is expressed on most commissural and longitudinal axons in the CNS and five motor nerve branches in the peripheral nervous system (PNS). However, Sema-1a-mediated axon guidance function contributes significantly to both intersegmental nerve b (ISNb) and segmental nerve a (SNa), and slightly to ISNd and SNc, but not to ISN motor axon pathfinding. Here, we uncover three cis-regulatory elements (CREs), R34A03, R32H10, and R33F06, that robustly drove reporter expression in a large subset of neurons in the CNS. In the transgenic lines R34A03 and R32H10 reporter expression was consistently observed on both ISNb and SNa nerve branches, whereas in the line R33F06 reporter expression was irregularly detected on ISNb or SNa nerve branches in small subsets of abdominal hemisegments. Through complementation test with a Sema-1a loss-of-function allele, we found that neuronal expression of Sema-1a driven by each of R34A03 and R32H10 restores robustly the CNS and PNS motor axon guidance defects observed in Sema-1a homozygous mutants. However, when wild-type Sema-1a is expressed by R33F06 in Sema-1a mutants, the Sema-1a PNS axon guidance phenotypes are partially rescued while the Sema-1a CNS axon guidance defects are completely rescued. These results suggest that in a redundant manner, the CREs, R34A03, R32H10, and R33F06 govern the Sema-1a expression required for the axon guidance function of Sema-1a during embryonic neural development.

• Biomolecules & Therapeutics
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• 제15권1호
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• pp.16-26
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• 2007

Tissue plasminogen activator (tPA) is a serine protease catalyzing the proteolytic conversion of plasminogen into plasmin, which is involved in thrombolysis. During last two decades, the role of tPA in brain physiology and pathology has been extensively investigated. tPA is expressed in brain regions such as cortex, hippocampus, amygdala and cerebellum, and major neural cell types such as neuron, astrocyte, microglia and endothelial cells express tPA in basal status. After strong neural stimulation such as seizure, tPA behaves as an immediate early gene increasing the expression level within an hour. Neural activity and/or postsynaptic stimulation increased the release of tPA from axonal terminal and presumably from dendritic compartment. Neuronal tPA regulates plastic changes in neuronal function and structure mediating key neurologic processes such as visual cortex plasticity, seizure spreading, cerebellar motor learning, long term potentiation and addictive or withdrawal behavior after morphine discontinuance. In addition to these physiological roles, tPA mediates excitotoxicity leading to the neurodegeneration in several pathological conditions including ischemic stroke. Increasing amount of evidence also suggest the role of tPA in neurodegenerative diseases such as Alzheimer's disease and multiple sclerosis even though beneficial effects was also reported in case of Alzheimer's disease based on the observation of tPA-induced degradation of $A{\beta}$ aggregates. Target proteins of tPA action include extracellular matrix protein laminin, proteoglycans and NMDA receptor. In addition, several receptors (or binding partners) for tPA has been reported such as low-density lipoprotein receptor-related protein (LRP) and annexin II, even though intracellular signaling mechanism underlying tPA action is not clear yet. Interestingly, the action of tPA comprises both proteolytic and non-proteolytic mechanism. In case of microglial activation, tPA showed non-proteolytic cytokine-like function. The search for exact target proteins and receptor molecules for tPA along with the identification of the mechanism regulating tPA expression and release in the nervous system will enable us to better understand several key neurological processes like teaming and memory as well as to obtain therapeutic tools against neurodegenerative diseases.

• 대한물리치료과학회지
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• 제8권1호
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• pp.745-758
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• 2001

The purpose of study to phenomenological examine and the mechanism regarding the gene(DNA, RNA, Protein) and sports to studied, analyzed. and evaluated. This review considers the evidence for genetic effects in several determinants of endurance performance and resistance performance, namely: body measurements and physique, body fat pulmonary functions, cardiac and circulatory functions, muscle characteristics. substrate utilization, maximal aerobic power and other. Moreover, the response to aerobic training of indicators aerobic work metabolism and endurance performance is reviewed, with emphasis on the specificity of the response and the individual differences observed in training ability. This study indicate that improvement of 'Enhancer Action' in RNA genes changed by exercise or sports. Moreover exercise was effect on Central Dogma with DNA makes RNA makes Protein. and think that occurred with exercise influence on skeletal muscle into cell have to Myosin Heavy Chain (MHC) changed was after exercise performance, which accompanied into skeletal muscle that were exercise-induces gene-modulation that is, take gene mutations. This study known that existed hormone(epinephrine)-immune system with interaction. Exercise were altered insulin binding and MAP Kinase signaling increased into immune cells. This review suggested that the high rate of glutamine utilization by cells of the immune system serves to maintain a high intra cellular concentration of the intermediates of biosynthetic pathways such that optimal rates of DNA, RNA and protein synthesis can be maintained. In the absence of glutamine, lymphocytes do not proliferate in vitro: proliferation increase greatly as the glutamine concentration increase. Glutamine is synthesized in skeletal muscle. Skeletal muscle and plasma glutamine levels are lowered by sepsis, injury, bums, surgery and endurance exercise and in the overtrained athlete. The study of result show that production of ET-1 is markedly increased tissue specifically in the heart by exercise without appreciable changes in endothelin-converting enzyme and endothelial receptor expressions, suggest that myocardial ET-1 may participate in modulation of cardiac function during exercise. Conclusionally, this study indicate that improvement of 'Enhancer Action' in RNA genes changed by exercise or sports. Moreover exercise was effect on Central Dogma with DNA makes RNA makes Protein. This study is expected to contribute the area of sports science, medicine, hereafter more effort is required to establish the relation between gene alters and exercise amount.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 1999년도 춘계종합학술대회
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• pp.237-242
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• 1999

본 논문에서는, 차수에 따라 체계적으로 변화하는 고차원펄스의 스펙트럼 특성을 이용하여 극초단 레이저펄스의 전송특성을 분석하였다. 부분응답시스템의 수정된 모델로부터 얻어지는 고차원펄스는 그 차수의 증가에 따라 FWHM폭이 현저히 감소하여 분석하고자 하는 극초단펄스의 형태에 근접하며, 그 스펙트럼과 전송대역폭도 차수에 따라 일률적으로 유도되므로 기존의 Gaussian 펄스나 Sech 펄스에 의한 근사화에 비하여 광범위하고 정확한 전송특성을 분석하는데 매우 유용함을 밝혔다. 우리는 부분응답시스템의 일반적 모델을 순환형으로 수정함으로써 정형화된 임의의 고차원펄스 형태를 얻어낼 수 있는 새로운 모델을 설계하였으며, 이를 이용하여 다양한 형태와 FWHM폭을 갖는 극초단 레이저펄스의 전송특성을 분석하는 새로운 방법을 제안하였다. 또한, 제안된 방법을 사용하여, 설정 펄스폭을 $\tau$=1(ps)으로 설정, 고차원펄스의 차수 n=1-100에서 얻어지는 FWHM 1(ps)-150(fs)의 극초단펄스의 스펙트럼을 제시하였고, 그 null-to-null 대역폭을 유도하였다. 전송특성은 레이저펄스의 보편적인 신호방식인 Unipolar 체계로 설정하여, 가능한 펄스간격에 따른 전력밀도스펙트럼을 유도하여 제시하였다. 이러한 결과는 기존의 실험결과와 일치함은 물론 레이저펄스 발생기술의 발달에 의하여 새로이 등장할 어떠한 형태와 폭을 갖는 극초단펄스에 대해서도 적용될 수 있다.

• 한국철도학회논문집
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• 제10권6호
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• pp.692-700
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• 2007

국내에서 개발된 한국형 틸팅열차는 현재 시제차량 제작을 마치고, 충북선을 시작으로 시운전 시험이 단계적으로 진행되고 있다. 시운전 시험에서는 한국형 틸팅열차의 시스템 안정화를 위해 차량 외에 궤도, 구조물, 전차선, 신호 등 각 시스템 상호간의 인터페이스 검증이 필요하며, 선로구축물 분야에서는 개발차량의 증속이 궤도구조에 미치는 영향을 비롯한 궤도부담력 검토를 통해 궤도구조의 안정성과 주행안정성 평가가 수행되어야 한다. 따라서 본 연구에서는 틸팅차량이 기존선 선로중 취약개소인 곡선부를 증속할 때 궤도에 미치는 영향을 검토하고자, 현재 시운전 시험이 진행되고 있는 충북선 및 호남선 구간 중 일부 선호를 대상으로 틸팅차량 주행이 기존선 궤도성능에 미치는 영향을 분석하였다. 틸팅열차, 고속열차 및 일반열차(무궁화, 화물)가 기존선 주행시 궤도각부의 거동특성을 파악하기 위해 윤중, 횡압, 레일 휨응력, 레일 수직 횡변위, 침목 수직변위 등을 측정하여 비교 분석함으로써 향후 기존선 속도향상을 위해 투입될 틸팅차량이 궤도에 미치는 영향을 평가하였다.

• 한국ITS학회 논문지
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• 제17권4호
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• pp.54-62
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• 2018

본 연구는 인공지능 신호 구현의 일환으로서, 딥러닝을 통해 실시간으로 추정하는 차량대기길이 기반의 감응식 신호 알고리즘을 제시하였다. 알고리즘의 구현을 위해 딥러닝 모형을 구현한 텐서플로우에 미시적 교통시뮬레이터인 Vissim을 제어하는 API, 즉 COM Interface를 구축하였다. Vissim에서 신호주기별로 수집된 링크통행시간과 통과교통량이 텐서플로우에 전달되면 학습이 완료된 딥러닝 모형을 통해 접근로별 차량대기길이가 추정된다. 접근로별 차량대기길이를 기반으로 신호시간을 산정한 후 Vissim 내부의 신호등화를 조정하여 시뮬레이션 한다. 본 연구에서 개발한 알고리즘은 현 TOD 방식에 비해 차량 지체가 약 5% 감소한 것으로 분석되었으며, 이는 네트워크 내 하나의 교차로만 대상으로 적용하여 그 효과가 제한된 것이며, 축 또는 네트워크 제어로의 공간적 확대방안을 향후연구로 제시하였다.