• Journal of Magnetics
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• 제18권1호
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• pp.14-20
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• 2013

For the treatment of osteoarthritis, pulsed electromagnetic field stimulus has been suggested as a useful therapeutic method in rehabilitative medicine. Most studies have been performed under low-frequency and low-energy to find out biological properties for stimulating chondrocyte with pulsed magnetic field. In this study, the effect of strong pulse magnetic field on the human chondrosarcoma cells (SW-1353) has been investigated by means of cell counting, morphologies, and gene expression of cartilage extracellular matrix genes. The SW-1353 cells were exposed under the field intensities of 270, 100, 55, 36, and 26 mTesla during 6 hours a day in 5 consecutive days. The pulse magnetic field with an LRC oscillating signal has the pulse width of 0.126 msec and stimulation period of 1 sec. For the 270 and 100 mTesla stimulation, the cell proliferation significantly increased in 21-24% as compared with the non-stimulated cells. Gene expression of cartilage extracellular matrix genes (ACAN, COMP and COL2A1) was assayed by quantitative real time-PCR method. The ACAN gene expression showed a significant brightness, which means the increase on gene expression, compared with the non-stimulated cells. Our results suggest that the strong pulse magnetic field stimulation can be utilized to accelerate cell proliferation and gene expression on human chondrosarcoma cells.

• Knee surgery & related research
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• 제30권4호
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• pp.303-310
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• 2018

Purpose: The purpose of this study was to investigate whether surgical experience could improve surgical competency in medial open wedge high tibial osteotomy (MOWHTO). Materials and Methods: One hundred consecutive cases of MOWHTO were performed with preoperative planning using the Miniaci method. Surgical errors were defined as under- or overcorrection, excessive posterior slope change, or the presence of a lateral hinge fracture. Each of these treatment failures was separately evaluated using the cumulative summation test for learning curve (LC-CUSUM). Results: The LC-CUSUM showed competency in prevention of undercorrection, excessive posterior slope change, and lateral hinge fracture after 27, 47, and 42 procedures, respectively. However, the LC-CUSUM did not signal achievement of competency in prevention of overcorrection after 100 procedures. Furthermore, the failure rate for overcorrection showed an increasing tendency as surgical experience increased. Conclusions: Surgical experience may improve the surgeon's competency in prevention of undercorrection, excessive posterior slope change, and lateral hinge fracture. However, it may not help reduce the incidence of overcorrection even after performance of 100 cases of MOWHTO over a period of 6 years.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2003년도 추계학술발표강연 및 논문개요집
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• pp.137-137
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• 2003

Optical amplificator have been used to compensate the losses in the optical signal transmission and processing. Today, there has been increasing demand for the very low cost optical amplifier. Sol-gel offers considerable potential both low cost manufacture, and for great flexibility in materials composition and structure. In addition, the sol-gel process is a very attractive method for producing porous materials with controlled structure. In this work, we present the potoluminescence properties of Er doped A1$_2$O$_3$/SiO$_2$ films. Erbium doped alumina nano sol was prepared by Al(NO$_3$)$_3$.9$H_2O$ and Er(NO$_3$)$_3$.5$H_2O$ through hydrolysis and peptization, and then GPS (3-Glycidoxypropyltrimethoxysilane) was added into Er doped alumina nano sol for organic- inorganic hybridization. Er doped A1$_2$O$_3$/SiO$_2$ film was obtained by spin coating, dip coating and thermal treatment from 30$0^{\circ}C$~120$0^{\circ}C$, and there were crack-free after thermal treatment. The thickness of film was measured SEM, and the porosity of film was characterized by BET and TGA. The crystal phase of Er doped A1$_2$O$_3$/SiO$_2$ were determined by XRD. Finally, the photoluminescence properties of Er doped A1$_2$O$_3$/SiO$_2$ films will be discuss with the consideration of porosity and crystallity.

• Nuclear Engineering and Technology
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• 제54권7호
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• pp.2592-2598
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• 2022

Research groups in the field of PET instrumentation are studying time-of-flight(TOF) technology to improve the signal-to-noise ratio of PET images. Scintillation light transport and collection plays an important role in improving the coincidence resolving time(CRT) of PET detector based on a pixelated crystal array. Four crystal arrays were designed by the different optical reflection configuration such as external reflectors and surface treatment on the CRT and compared with the light output, energy resolution and CRT. The design proposed in the study was composed of 8 × 8 LYSO crystal array consisted of 3 × 3 × 15 mm³ pixels. The entrance side was roughened while the other five surfaces were polished. Four sides of all crystal pixels were wrapped with ESR-film, and the entrance surface was covered by Teflon-tape. The design provided an excellent timing resolution of 210 ps and improved the CRT by 16% compared to the conventional method using a polishing treatment and ESR-film. This study provided a method for improving the light output and CRT of a pixelated scintillation crystal-based brain TOF PET detector. The proposed configuration might be an attractive detector design for TOF brain PET requiring fast timing performance with high cost-effectiveness.

• Nutrition Research and Practice
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• 제14권5호
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• pp.463-477
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• 2020

BACKGROUND/OBJECTIVES: Many studies have suggested that Rhus verniciflua Stokes (RVS) and its extract are anticancer agents. However, RVS had limited use because it contains urushiol, an allergenic toxin. By improving an existing allergen-removal extraction method, we developed a new allergen-free Rhus verniciflua Stokes extract (RVSE) with higher flavonoid content. In this study, we examined whether RVSE inhibits the ability of AGS gastric cancer cells to migrate and invade. MATERIALS/METHODS: The flavonoids content of RVSE was analyzed by HPLC. The effects of RVSE on migration and invasion in AGS cells were analyzed by each assay kit. Matrix metalloproteinase (MMP)-9, plasminogen activator inhibitor-1 (PAI-1) and urokinase-type plasminogen activator (uPA) protein expression was analyzed by protein antibody array. The Phosphorylation of signal transducer and activator of transcription (STAT) 3 were assayed by Western blot analysis. RESULTS: RVSE treatment with 0-100 ㎍/mL dose-dependently reduced the ability of AGS cells to migrate and invade. Notably, treatment with RVSE strongly inhibited the expression of MMP-9 and uPA and the phosphorylation of STAT3. In contrast, RVSE treatment dramatically increased the expression of PAI-1. These results indicate that the inhibition of MMP-9 and uPA expression and STAT3 phosphorylation and the stimulation of PAI-1 expression contributed to the decreased migration and invasion of AGS cells treated with RVSE. CONCLUSIONS: These results suggest that RVSE may be used as a natural herbal agent to reduce gastric cancer metastasis.

• 한국방사선학회논문지
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• 제13권7호
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• pp.941-946
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• 2019

환자의 진단 및 치료를 결정할 때 전산화단층촬영 (computed tomography; CT)은 우수한 해부학 정보를 제공하기 때문에 진단에 큰 도움이 되어 있다. 그에 따른 사용빈도가 증가하고 있으며, 활용 및 응용범위도 확대되어가는 추세이다. 저관전압을 사용해서 CT 검사를 하면 노이즈가 증가한다. 기존의 여러 연구에서는 영상재구성법에 대한 노출조건을 조절하는 방법 등을 시도해서 최적의 화질을 찾는 방법을 찾고 있으나 근본적 문제해결은 되지 못한다. 입력 영상의 신호를 유지하고 노이즈만을 최대한 제거하기 위해서 이중 트리웨이블릿 알고리즘을 적용하였다. 실험결과 100kVp, 회전시간 0.5sec의 영상에서 complex oriented 2d 방을 사용 할 경우 노이즈는 8.53에서 4.51로 줄어들었다. 본 연구를 통해서 저관전압 두부 CT에서 발생하는 높은 수준의 노이즈를 최적의 노이즈제거 알고리즘으로 노이즈를 제거하고 환자선 량을 낮출 수 있었다. 본 연구결과를 임상에서 사용 시 저선량의 CT 사용이 가능하고 환자 피폭을 줄일 것으로 판단한다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.657-662
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• 2008

To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, the recombinant DgHSP17.2 gene was introduced into orchardgrass plants using the Agrobacterium-mediated transformation method and expressed constitutively under the control of the CaMV 35S promoter. The results of genomic DNA PCR and Southern analysis showed a DNA band and hybridization signal on agarose gel and X-ray film in transgenic orchardgrass plants harboring the recombinant DgHSP17.2 gene, but a DNA band and hybridization signal were not observed in the wild type and empty vector control plants. The same result was also obtained in RT-PCR and Southern blot analysis, and these transgenic orchardgrass plants did not show any morphological aberration both in the culture bottle and soil mixture. When leaf discs cut from transgenic orchardgrass plants with recombinant DgHsp17.2 gene were exposed to lethal temperature (heat treatment at $60^{\circ}C$ for 50 min), 60-80% of the leaf discs showed only damage symptoms, but non-transgenic leaf discs showed a lethal condition. These results indicate that the DgHsp17.2 gene may act as a protector from heat stress in plants.

• 융합신호처리학회논문지
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• 제20권2호
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• pp.70-77
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• 2019

조기 유방암을 진단하기 위해서는 유방초음파 판독이 매우 중요하다. 초음파 검사는 초음파장비에 따라 화질의 차이가 심하게 나타날 뿐만 아니라 검사자의 경험과 숙련 정도에 따라 진단의 차이가 크게 나타난다. 따라서 정확한 진단과 치료를 위하여 객관적인 판단기준이 필요하다. 이에 본 연구에서는 GLCM(Gray Level Co-occurrence Matrix) 알고리듬을 적용하여 질감 특성을 분석하고 특징파라미터들을 추출하여 신경망분류기를 이용하여 유방암을 진단하였다. 유방초음파 영상은 정상 조직과 양성, 악성 종양으로 분류하여 질감 특성 파라미터 6가지를 추출하였다. 유방초음파검사로 진단된 정상 영상, 악성 및 양성종양 영상 각각 14증례를 대상으로 추출된 6개의 파라미터들을 적용하여 다층 퍼셉트론 신경망구조 역전파 학습방법으로 학습을 시켰다. 학습된 모델에 정상 유방 영상 51증례, 양성종양 영상 62증례, 악성종양 영상 74증례의 영상을 사용하여 분류한 결과 95.2%의 분류율을 나타내었다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.69.1-69
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• 2003

RSH (relA/spoT homolog) has been known to determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotide of the prokaryotic stringent response and also play a role in antibiotic production and differentiation in Streptomyces species but not a little in eukaryotic organism, especially in plant. Salicylic acid (SA), a critical signal molecule of establishing systemic acquired resistance (SAR), could induce SAR in Pepper (Capcicum annuum) against Phytophthora capsici. And the extent of SAR induction was in proportion to the dosage of SA (or BTH). Suppression subtractive hybridization (SSH), a PCR-based method for cDNA subtraction, was carried out between SA-treated and non-SA-treated pepper leaves to isolate genes which may be responsible for defense signaling against pathogens. Early upregulated gene was selected from reverse northern and kinetics of SSH-genes transcripts in SA-treated pepper leaves upon SA treatment. Full-length cDNA of the gene (PepRSH； Pepper RelA / SpoT homolog) had an open reading frame (ORF) of 2166 bp encoding a protein of 722 amino acids and a significant homology with (p)ppGpp phosphohydrolase or synthetase. Genomic DNA gel blot analysis showed that pepper genome has at least single copy of PepRSH. PepRSH transcripts was very low in untreated pepper leaves but strongly induced by SA and methyljasmonic acid (MeJA), indicating that PepRSH may share common SA and MeJA-mediated signal transduction pathway Functional analysis in E. coli showed PepRSH confers phenotypes associated with (p)ppGpp synthesis through a complementation using active site mutagenesis.

• 한국항해항만학회지
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• 제28권3호
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• pp.239-245
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• 2004

심전도 측정기와 청진기를 통하여 획득된 심전도와 심음도는 비관혈적 방법으로 환자를 진단할 수 있는 생체신호이며, 의사들의 진단도구로 널리 이용되어져 왔다. 그러나 의사들이 심전도 측정기나 청진기를 이용하여 환자를 진단하는 장소는 병원 내 진료실과 응급실로 한정되어 있기 때문에, 병원에서 거리가 먼 원거리 지역이나 자동차, 비행기, 선박에서는 환자의 심음을 확인할 수 없다. 특히, 원양항해에 종사하는 선박 환자는 전문 의료진으로부터 진료를 받을 수 없는 경우가 있다. 본 논문에서는 환자의 심음 및 심전을 용이하게 측정함으로써 PC기반의 선박용 디지털 심전도 및 청진기를 개발하였으며, 해상 원격진료의 출현을 앞 당길 수 있으리라 본다.