• BMB Reports
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• v.33 no.2
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.11 no.1
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• pp.18-25
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• 2018

In this paper, we present a low-power implementation of the multi-channel hearing aid system using a general-purpose DSP chip. The system includes an acoustic amplification algorithm based on Wide Dynamic Range Compression (WDRC), an adaptive howling canceller, and a single-channel noise reduction algorithm. To achieve a low-power implementation, each algorithm is re-constructed in forms of integer program, and the integer program is converted to the assembly program using BelaSigna(R) 250 instructions. Through experiments using the implementation system, the performance of each processing algorithm was confirmed in real-time. Also, the clock of the implementation system was measured, and it was confirmed that the entire signal processing blocks can be performed in real time at about 7.02MHz system clock.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.720-725
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• 2015

This paper describes the design of a wideband cascode amplifier using a feedback network and microwave small-signal transistors. The adopted cascode structure enables the miller effect to be lessened, cutoff frequency to increase, and reduction of gain in the mid-band to be mitigated. In addition, a feedback network is added to the cascode structure to improve the input matching and ripple performances over the wide operating band. The designed cascode amplifier contains a feedback network for small size and broadband amplification, whereas balanced amplifiers and distributed amplifiers have been used widely. The measurement shows $8.5dB{\pm}1.5dB$ of gain over 1000-2000MHz. The fabricated cascode amplifier has more than 8dB of gain over a 1000MHz bandwidth with a good flatness. The measured performances agree with the predicted ones even a minor shift in operating frequency is observed.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1094-1099
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• 2005

Photosynthetic dinoflagellates contain a water-soluble, light-harvesting antenna called the peridinin-chlorophyll-protein (PCP) complex, which has an apoprotein with no sequence similarity to other known proteins. There are two forms of PCP apoproteins; the 15-kDa short form and the 32- to 35­kDa long form. The present study describes the PCP protein and its cDNA from Alexandrium tamarense. A cDNA library was constructed from mRNA isolated from A. tamarense. The complete PCP cDNA was generated by reverse-transcription coupled to polymerase chain reaction (RT-PCR), together with rapid-amplification of cDNA ends (RACE). The A. tamarense PCP cDNA encoded a 55-amino acid signal peptide and a 313-amino acid mature protein with a calculated mass of 32 kDa, which corresponded to that of the long form of PCP. Phylogenetic analysis indicated that the sequence of A. tamarense PCP did not cluster with the short-form PCPs, to which it was only about $55\%$ identical, but which were $79-83\%$ identical to other long-form PCPs. The deduced amino acid sequence of A. tamarense PCP contains an internal duplication, which suggests the possibility that long-form PCPs arose by gene duplication or by the fusion of genes encoding the short form. The abundance of PCP mRNA changed substantially in response to different light conditions, indicating the possible existence of a photo-acclimation response in A. tamarense.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.1
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• pp.245-252
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• 2014

Human METCAM/MUC18, a cell adhesion molecule (CAM) in the immunoglobulin-like gene super family, plays a dual role in the progression of several epithelium cancers; however, its role in the nasopharyngeal carcinoma (NPC) remains unclear. To initiate the study we determined human METCAM/MUC18 expression in tissue samples of normal nasopharynx (NP), NPCs, and metastatic lesions, and in two established NPC cell lines. Immunoblotting analysis was used for the determination in lysates of frozen tissues, and immunohistochemistry (IHC) for expression in formalin-fixed, paraffin-embedded tissue sections of 7 normal nasopharynx specimens, 94 NPC tissue specimens, and 3 metastatic lesions. Human METCAM/MUC18 was expressed in 100% of the normal NP, not expressed in 73% of NPC specimens (or expressed at very low levels in only about 27% of NPC specimens), and expressed again in all of the metastatic lesions. The level of human METCAM/MUC18 expression in NPC tissues was about one fifth of that in the normal NP and metastatic lesions. The low level of human METCAM/MUC18 expression in NPC specimens was confirmed by a weak signal of RT-PCR amplification of the mRNA. Low expression levels of human METCAM/MUC18 in NPC tissues were also reflected in the seven established NPC cell lines. These findings provided the first evidence that diminished expression of human METCAM/MUC18 is an indicator for the emergence of NPC, but increased expression then occurs with metastatic progression, suggesting that huMETCAM/MUC18, perhaps similar to TGF-${\beta}$, may be a tumor suppressor, but a metastasis promoter for NPC.

• Journal of Power Electronics
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• v.15 no.6
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• pp.1673-1681
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• 2015

A low dropout (LDO) regulator with a wide-bandwidth is proposed in this paper. The regulator features a Human Body Model (HBM) 8kV-class high robustness ElectroStatic Discharge (ESD) protection circuit, and two error amplifiers (one with low gain and wide bandwidth, and the other with high gain and narrow bandwidth). The dual error amplifiers are located within the feedback loop of the LDO regulator, and they selectively amplify the signal according to its ripples. The proposed LDO regulator is more efficient in its regulation process because of its selective amplification according to frequency and bandwidth. Furthermore, the proposed regulator has the same gain as a conventional LDO at 62 dB with a 130 kHz-wide bandwidth, which is approximately 3.5 times that of a conventional LDO. The proposed device presents a fast response with improved load and line regulation characteristics. In addition, to prevent an increase in the area of the circuit, a body-driven fabrication technique was used for the error amplifier and the pass transistor. The proposed LDO regulator has an input voltage range of 2.5 V to 4.5 V, and it provides a load current of 100 mA in an output voltage range of 1.2 V to 4.1 V. In addition, to prevent damage in the Integrated Circuit (IC) as a result of static electricity, the reliability of IC was improved by embedding a self-produced 8 kV-class (Chip level) ESD protection circuit of a P-substrate-Triggered Silicon Controlled Rectifier (PTSCR) type with high robustness characteristics.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.12
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• pp.1-7
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• 2010

In this paper, we suggested a sensing method of PoRAM with the multilevel cell When a specific voltage is applied between top and bottom electrodes of PoRAM unit cell, we can distinguish cell states by changing resistance values of the cell. Especially, we can use the PoRAM as the multilevel cell due to have four stable resistance values per cell. Therefore, we proposed an address decoding method, sense amplifier and control signal for sensing of a multilevel cell. The sense amplifier is designed based on a current comparator that compared a cell current the cell with a reference current, and have a low input impedance for a amplification of the current. The proposed circuit was designed in a $0.13{\mu}m$ CMOS technology, we verified to sense each data "00", "01", "10", "10" by four states of a cell current.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.6A
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• pp.499-509
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• 2005

The capacity of Satellite DMB(Digital Multimedia Broadcasting) system is limited mainly by the interference. So, to achieve the expected performance of Satellite DMB system and to minimize the interference from other Satellite DMB system, ACI(Adjacent Channel Interference) should be considered carefully. Satellite DMB system uses the Gap-filler for effective transmission in terrestrial environment, and the Gap-filler can use direct amplification or frequency conversion to satisfy the specific requirements. Therefore, amplified signal causes several effects on interference between System A(Eureka 147 DAB) and System E(ISDB : Integrated services Digital Broadcasting). In this paper, by using the outcome of system-level simulation considering the results of link-level simulation, we analyze the interferences between System A and System E under practical situation based on the exact parameters of ITU-R BO. 1130-4. We also propose the appropriate level of guard band and Cell Radius to optimize system capacity by adapting the spectrum mask given in the spec. and utilizing the interference analysis between System A and System E.

• Journal of the Korea Society of Computer and Information
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• v.16 no.2
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• pp.17-23
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• 2011

In this paper, daily life stress monitoring system is proposed. The proposed wireless ECG module, reducing the noise and increasing the size of signal, amplification circuit was designed for. Using HRV(Heart Rate Variability), extracted by measuring R-wave, stress diagnostic algorithms to assess the stress of human emotion were developed. For monitoring the activities, the proposed system is consist of small rectangular size for portable and by simple measurement it is possible to measure at any time. Through experiments, the proposed approach to represent user's stress level can be confirmed. Through that, it can see appropriate structure to obtain R-wave for stress assess as well as high resemblance to the clinical electrocardiogram. In this paper, performed experiments was developed nonrestraint measuring and wearable wireless biometric scanner that is able to monitor the heart's electrical activity of everyday life.Using this, the algorithm system, that is able to assess stress index through time-domain and frequency-domain analysis of the front and the rear of performing stress load protocol, was developed,

• Journal of the Korean Society for Nondestructive Testing
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• v.35 no.2
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• pp.128-133
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• 2015

This paper describes the construction of a static 3D ultrasonography image by tracking the radiation beam position during the handy operation of a 1D array probe to enable point-of-care use. The theoretical model of the transformation from the translational and rotational information of the sensor mounted on the probe to the reference Cartesian coordinate system was given. The signal amplification and serial communication interface module was made using a commercially available sensor. A test phantom was also made using silicone putty in a donut shape. During the movement of the hand-held probe, B-mode movie and sensor signals were recorded. B-mode images were periodically selected from the movie, and the gray levels of the pixels for each image were converted to the gray levels of 3D voxels. 3D and 2D images of arbitrary cross-section of the B-mode type were also constructed from the voxel data, and agreed well with the shape of the test phantom.