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Application of the QLF technology to monitor recovery rates of enamel caries lesions with human saliva (법랑질 병소 회복율 평가를 위한 QLF 기술의 적용)

  • Kim, Gyung-Min;Ku, Hye-Min;Lee, Eun-Song;Kang, Si-Mook;Jong, Elbert de Josselin de;Kwon, Ho-Keun;Kim, Baek-Il
    • The Journal of the Korean dental association
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    • v.55 no.2
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    • pp.156-164
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    • 2017
  • Purpose: The aim of this in vitro study was to assess changes in remineralization by stimulated human saliva over a short period of 48 hours with quantitative light-induced fluorescence (QLF) technology. Materials and Methods: Bovine incisor surfaces were demineralized for 10 days. Two types of stimulated saliva were collected from 7 healthy persons. 24 hours after tooth brushing (Stimulated saliva group) and immediately after tooth brushing with 1,000 ppm NaF dentifrice (Dentifrice saliva group). The specimens were immersed in saliva and fluorescence images were obtained by QLF-digital (QLF-D $biluminator^{TM}$,) at 2, 4, 6, 12, 24, and 48 hours fluorescence loss (${\Delta}F%$) of the lesions. A paired t-test was performed to assess fluorescence differences between before (${\Delta}F_{baseline}$) and after (${\Delta}F_{treatment\;time}$) the remineralization process. Results: Before the remineralization, the mean ${\Delta}F_{baseline}$ of the initial demineralized specimens was $-18.42{\pm}0.15$ (%). In both groups, the ${\Delta}F$ values obtained at baseline and after 2 hours were statistically significant (P < 0.001), indicating recovery of the lesions by approximately 40% after 2 hours. After 48 hours, remineralization rates were slightly higher (49%) for the stimulated saliva group than for the dentifrice saliva group (41%), but the difference was not statistically significant. Conclusions: With QLF minute degrees of remineralization by saliva can be measured in periods as short as 2 hours. Additionally no significantly higher effects of remineralization were observed in the dentifrice saliva group when compared to the stimulated saliva group.

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Wastewater Treatment Characteristics by Pseudomonas sp. BLP2052 and Flavobacterium sp. BLP20515 Isolated from Sewage (선별된 Pseudomonas sp. BLP2052와 Flavobacterium sp. BLP20515의 폐하수 처리 특성)

  • 박철환;최광근;임지훈;이상훈;김상용;이진원
    • KSBB Journal
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    • v.14 no.2
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    • pp.153-159
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    • 1999
  • Fifteen microbes have been isolated from Jangja pond in Kuri-Si, Kyeonggi-Do. Among them, two strains showed excellent COD removal from wastewater, which were named Pseudomonas sp. BLP2052 and Flavobacterium sp. BLP20515, respectively. Optimal pH and temperature for the cell growth were 7.0 and $30^{\circ}C$ for both strains. Pseudomonas sp. BLP2052 and Flavobacterium sp. BLP20515 was applied to the reactor to treat wastewater and 66.0% and 65.7% COD (chemical oxygen demand) removal was achieved, respectively. Comparing these results to the case of applying mixed microbes present in Jangja pond, COD removal rate was 15% less. But when adding the selected microbes to the wastewater containing mixed microbes, COD removal rate increased by 5%. After 84 hour operation, we achieved 85.6% COD removal. When inhibitors were added less than 100 ppm, during the microbial wastewater treatment, Fe, Zn, Al, phenol and Cr influenced microbial activity more deterioratively in order. In the case of over 300 pm, Cr, Fe, Zn, Al and phenol showed severe deteriorative effect in order.

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Preference and Eating Activities of Chinese for Traditional Korean Kimchi (한국 전통 김치에 대한 중국인들의 기호도와 소비행태에 관한 연구)

  • Kim, Eun-Mi;Kim, Young-Jin;Jeong, Mi-Kyoung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.10
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    • pp.1641-1645
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    • 2004
  • The purpose of this research is to investigate Chinese's preference and directions for improvement of Kimchi by sensory evaluation, and utilize Kimchi for the China eating habit by survey. Self administered questionnaires were collected 149 Chinese (16~25 yr) residing in Shanghai. About 89.9% of all the participants had eaten Kimchi before the survey. Overall preference about Kimchi was 3.59$\pm$0.62 (5 point: Lickertis scale) and affecting factors for overall preference were taste, appearance, texture, in that order. Chinese answered that tough texture (24.9%), spicy taste (20.5%) and fermented odor (18.1%) were the most important factors in improved quality of Kimchi. They answered that applications of Kimchi with Chinese cuisine are 'side dish with meat cuisine', 'eat with cooked rice, rice-mixed-in-soup, gruel' and 'use ingredients of cook', as similar to Korean's.

Determination of Glucose Distribution of Potato Tuber Using Blood Glucose Meter and Its Application to Estimate Processing Quality (혈당측정기를 이용한 감자 괴경의 포도당 분포 분석과 이의 가공적성 평가에의 활용)

  • Kwon, Byoung-Hoon;Kim, Si Un;Lim, Soo Yeon;Namgung, Hyeju;Lee, Hong Jin
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.171-177
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    • 2014
  • In an effort to investigate special distribution of glucose content of potato tuber of a cultivar 'Superior' after harvest, a whole tuber was longitudinally cut into halves and cut-surface was divided into 11 regions. Approximate glucose concentration of each section was determined using a commercial blood glucose meter. Higher level of glucose was detected in the outer layers of tissue than inner part of tuber although there were lower coefficients of variation value, 37.4% and 34.1% among individual tubers and among defined sections, respectively. A positive correlation between the whole tuber and individual section was existed in glucose content, where the central pith tissue gave the highest coefficient (r = 0.921) and bud end tissue did the lowest (r = 0.544). Glucose content of the tubers stored for 4 months at $2.0^{\circ}C$ was 5.5 fold higher compared to the tubers kept in ambient temperature. The chip color of the former tubers was much brighter than those of the latter tubers. The result obtained in the present study suggests that the blood glucose meter can be used to a rapid and simple evaluation of glucose content and therein be applied to estimate the processing quality of potato tubers during postharvest handling.

Macronutrient composition of human milk from Korean mothers of full term infants born at 37-42 gestational weeks

  • Chang, Namsoo;Jung, Ji A;Kim, Hyesook;Jo, Ara;Kang, Sujeong;Lee, Si-Won;Yi, Hyunju;Kim, Jihee;Yim, Jong-Gap;Jung, Byung-Moon
    • Nutrition Research and Practice
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    • v.9 no.4
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    • pp.433-438
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    • 2015
  • BACKGROUND/OBJECTIVES: Breast milk is the best available food for optimum growth and development of infants and the breastfeeding rate is increasing in Korea. The purpose of this study is to measure the concentrations of macronutrients and to evaluate their changes according to lactation period in breast milk from lactating Korean women. SUBJECTS/METHODS: Milk samples were obtained from 2,632 healthy lactating women (mean age; $32.0{\pm}3.3years$), where the lactating period was up to a period of 8 months, who also volunteered to participate in the Human Milk Macronutrient Analysis Research. Lactose, protein, fat and water content in the breast milk samples were analyzed with infrared spectrometry using MilkoScan FT-2. RESULTS: The mean macronutrient composition per 100 mL of mature breast milk was 7.1 g for lactose, 1.4 g for protein and 3.0 g for fat, and energy content was 61.1 kcal. The protein concentration was significantly lower in milk samples at 1-2 weeks (2.0 g/dL) to 2-3 months (1.4 g/dL) than those at 0-1 week (2.2 g/dL), but it was similar among samples from 3-4 months to 7-8 months (1.3 g/dL). Mean lipid levels varied among different lactational period groups (2.7-3.2 g/dL), but presented no significant difference. Lactose concentration in the milk samples did not differ with lactation period. Maternal body mass index was positively related to protein and lipid breast milk contents, but was negatively related to lactose content. General linear models examining the associations between maternal variables and milk macronutrient content revealed that lactation period had a major impact on protein and lipid, but not on lactose content in breast milk. CONCLUSIONS: These results warrant future studies to explore factors that may be associated with changes in macronutrient content in human milk.

Effects of Sa-am Acupuncture Treatment on a ALS(Amyotrophic Lateral Sclerosis) Patient (근위축성 측삭 경화증 환자 1례를 대상으로 한 사암침법의 효과)

  • Lee, Sang-Mi;Kim, Sung-Ha;Jeong, Ho-Hyun;Park, Man-Yong;Lee, Jeong-Ju;Park, Ok-Ju;Lim, Jin-Young;Kim, Dong-Woung;Song, Bong-Keun;Kim, Sung-Chul
    • Journal of Acupuncture Research
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    • v.29 no.5
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    • pp.187-195
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    • 2012
  • Objectives : Amyotrophic lateral sclerosis(ALS) is a progressive and incurable disease that causes degeneration of the motor neurons of the brain stem and spinal cord. The purpose of this study was to report the effectiveness of Sa-am acupuncture treatment on ALS patient. Methods : Following the stabilization period, a ALS patient respiratory figures(Et $C_{O2}$, Sp $_{O2}$, RR, pulse) were measured by using capnography & pulse oximetry for 30 minutes before acupuncture treatment. Acupuncture treatment such as lung tonification($SP_3(+){\cdot}LU_9(+){\cdot}HT_8(-){\cdot}LU_{10}(-)$), heart tonification($HT_9(+){\cdot}LR_1(+){\cdot}SI_8(-){\cdot}KI_{10}(-)$), liver tonification ($KI_{10}(+){\cdot}LR_8(+){\cdot}LU_8(-){\cdot}LR_4(-)$) were conducted for 5 days. Each Sa-am acupuncture treatment conducted at AM 7 : 00, AM 11 : 00, PM 4 : 30 of the day. During acupuncture treatment, the patient respiratory figures were measured equally for 30 minutes. The patient was treated by using Dong-bang acupuncture needles($0.30{\times}4.0$) and also stimulated using infra red. Results : The value of Et $C_{O2}$ has decreased more after Sa-am acupuncture lung tonification treatment than others. The value of pulse has decreased more after Sa-am acupuncture heart tonification treatment than others. The value of raspiration rate has decreased more after Sa-am acupuncture liver tonification treatment than others. And the value of Sp $O_2$ has increased more after Sa-am acupuncture liver tonification treatment than others. Conclusions : Although this study was subject to a few limitations, but it shows that Sa-am acupuncture treatment for ALS patients has a meaningful effect. This study needs to be developed further using a larger sample size to obtain more valuable and meaningful data.

Characteristics of Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides (Leuconostoc mesenteroides에서 分離한 Glucose-6-phosphate Dehydrogenase의 特性)

  • Byun Si Myung;Yang Do Choi;Moon H. Han
    • Journal of the Korean Chemical Society
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    • v.23 no.4
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    • pp.248-258
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    • 1979
  • Glucose 6-phosphate dehydrogenase of Leuconostoc mesenteroides which was purifid by an affinity chromatography was studied on the characterization, kinetics and chemical modification. The apparent molecular weight of the enzyme was 112,000 by the gel filtration method of Sephadex G-200 column. The optimum temperature of $NAD^+$-linked reation was 50$^{circ}C$ and the activation energy and the heat of inactivation were 8.36 kcal/mole and -58.2kcal/mole, respectively. The steady state kinetic study showed KG6P, Kemp, and CX KNADP to be 76.9 PM, 7.46${\mu}M$ and 7.14 ${\mu}M$, respectively, and KGGP, KNAD,and aKNm to be 53.7${\mu}M$, 115.2${\mu}M$ and 702.2${\mu}M$ for the $NAD^+$-linked reaction at pH 7.8, optimum pH. The pH dependent kinetic constants suggested that the two ionizing groups whose pKa is 7.2 .and pKb is 9.0-9.6 were involved in the enzyme-substrate interaction. Evidence by photooxidation and carboxymethylation of the enzyme suggested that the imidazole group of histidine with pKa group may participate in the catalytic site.

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Nutritional status of patients treated with radiotherapy as determined by subjective global assessment

  • Koom, Woong Sub;Ahn, Seung Do;Song, Si Yeol;Lee, Chang Geol;Moon, Sung Ho;Chie, Eui Kyu;Jang, Hong Seok;Oh, Young-Taek;Lee, Ho Sun;Keum, Ki Chang
    • Radiation Oncology Journal
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    • v.30 no.3
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    • pp.132-139
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    • 2012
  • Purpose: The purpose of this prospective multi-institutional study was to evaluate the nutritional status of patients undergoing radiotherapy (RT) for treatment of head and neck, lung, or gastrointestinal cancer. Materials and Methods: A total of 1,000 patients were enrolled in this study at seven different hospitals in Seoul, Korea between October 2009 and May 2010. The nutritional status of patients after receiving 3 weeks of RT was evaluated using subjective global assessment (SGA). The nutritional status of each patient was rated as well nourished (A), moderately malnourished (B), or severely malnourished (C). Results: The mean age of patients in this study was $59.4{\pm}11.9$ years, and the male to female ratio was 7:3. According to the SGA results, 60.8%, 34.5%, and 4.7% of patients were classified as A, B, or C, respectively. The following criteria were significantly associated with malnutrition (SGA B or C; p < 0.001): loss of subcutaneous fat or muscle wasting (odds ratio [OR], 11.473); increased metabolic demand/stress (OR, 8.688); ankle, sacral edema, or ascites (OR, 3.234); and weight loss ${\geq}5%$ (OR, 2.299). Conclusion: SGA was applied successfully to assess the nutritional status of most patients. The prevalence of malnutrition in a radiation oncology department was 39.2%. The results of this study serve as a basis for implementation of nutrition intervention to patients being treated at radiation oncology departments.

Passive Alignment of Photodiode by using Visible Laser and Flip Chip Bonding (가시광 레이저를 이용한 수광소자의 수동정렬 및 플립칩본딩)

  • Yu, Chong-Hee;Lee, Sei-Hyoung;Lee, Jong-Jin;Lim, Kwon-Seob;Kang, Hyun-Seo
    • Journal of the Microelectronics and Packaging Society
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    • v.14 no.3
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    • pp.7-13
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    • 2007
  • In the optical module for optical communications, the flip chip bonding is used fer the precise alignment of the optical fiber and optical device. In flip chip bonding, the optical device is aligned and welded while observing the alignment mark of substrate and chip by using flip chip bonder in order to bond the optical device at the exact position. In this research, optical passive alignment method of photodiode(PD) flip chip bonding is suggested for low cost optical subassembly. By using the visible He-Ne laser (633nm wavelength), photodiode is easily aligned with emitting spot on the optical fiber with the help of stereoscopic alignment system. We compensated wavelength dependent deviation about 4m to find out real alignment position of 1550nm input laser by ray tracing. The maximum optical coupling efficiency between the optical fiber and photodiode was about 23.3%.

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Improvement effect of total nitrogen and amino acid content in spent mushroom substrates by bacterial treatment (세균을 이용한 수확후배지의 총질소 및 아미노산 증진 효과)

  • Baek, Il-Sun;Kim, Jeong-Han;Lee, Yong-Seon;Shin, Bok-Eum;Lee, Young-Soon
    • Journal of Mushroom
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    • v.16 no.3
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    • pp.225-230
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    • 2018
  • This study was conducted to reuse spent mushroom substrates (SMS) of Pleurotus ostreatus and improve their nitrogen content by bacterial treatment. Two kinds of bacteria were used to investigate the increase in total nitrogen (T-N) content. Bacillus sp. (GM20-4) was isolated from SMS of oyster mushroom, and Rhodobacter sphaeroides (RS) was obtained from Gwangju Si Agricultural Technology Center. SMS samples were collected from three oyster mushroom cultivation farms located in Gyeonggi-do province, Korea. When dried SMS was inoculated with 30% culture broth of GM20-4 and RS and incubated at room temperature ($25{\pm}2^{\circ}C$) for 5 days, T-N content increased. To investigate the T-N content of other SMS, three dried SMS samples (A, B, and C) were treated by the same method using GM20-4 and RS. As a result, the T-N content of sample B was 20% higher than that of the control, whereas the T-N content of samples A and C increased to 17% and 12%, respectively. The change in T-N content by bacterial treatment of wet SMS was slightly higher than that of the control. The changes in amino acid content were also found to be higher than those in the control in all SMS samples by GM20-4 and RS treatment. Aspartic acid and glutamic acid contents were the highest among all amino acid compositions. Especially, the aspartic and glutamic acid contents of sample B increased by 2.9 folds higher than the control.