• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.489-498
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• 2018

Objective: Foot and mouth disease (FMD) and porcine reproductive and respiratory syndrome (PRRS) are major diseases that interrupt porcine production. Because they are viral diseases, vaccinations are of only limited effectiveness in preventing outbreaks. To establish an alternative multi-resistant strategy against FMD virus (FMDV) and PRRS virus (PRRSV), the present study introduced two genetic modification techniques to porcine cells. Methods: First, cluster of differentiation 163 (CD163), the PRRSV viral receptor, was edited with the clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 technique. The CD163 gene sequences of edited cells and control cells differed. Second, short hairpin RNA (shRNAs) were integrated into the cells. The shRNAs, targeting the 3D gene of FMDV and the open reading frame 7 (ORF7) gene of PRRSV, were transferred into fibroblasts. We also developed an in vitro shRNA verification method with a target gene expression vector. Results: shRNA activity was confirmed in vitro with vectors that expressed the 3D and ORF7 genes in the cells. Cells containing shRNAs showed lower transcript levels than cells with only the expression vectors. The shRNAs were integrated into CD163-edited cells to combine the two techniques, and the viral genes were suppressed in these cells. Conclusion: We established a multi-resistant strategy against viral diseases and an in vitro shRNA verification method.

• 조명전기설비학회논문지
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• 제21권7호
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• pp.20-28
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• 2007

본 논문에서는 2회선 가공송전선에 대하여 2회선 중 1회선에 고장이 발생한 경우에 안전하게 운전할 수 있는 단시간정격을 결정하기 위한 해석적 방법을 기술한다. 도체의 온도특성을 나타내는 열평형방정식을 선형화 함으로써 이 선형방정식으로 1회선 선로에 과부하가 발생하는 동안 과전류와 그 온도 특성을 쉽게 표현할 수 있다. 일반적으로 단시간 선로정격은 도체의 수명과 이도를 동시에 고려하여 결정해야 한다는 것은 잘 알려진 사실이다. 그렇지만 대부분의 전력회사는 단시간정격에 대한 각자의 다른 지침을 갖고 있다. 이 논문에서는 제안된 방법을 사용하면 과거 3시기에 건설된 한국전력공사의 가공송전선에 규정된 단시간정격을 재평가할 수 있고, 그 결과 단시간정격을 효율적으로 증대시킬 수 있음을 확인하였다. 또한 이 방법은 2회선 선로 중 1회선에 고장이 발생할 경우에 기존 선로에 어떤 조치도 하지 않아도 단시간 동적송전용량을 결정하는데 직접 이용할 수 있을 것이다.

• 대한물리의학회지
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• 제12권1호
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• pp.93-101
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• 2017

PURPOSE: The purpose of this study was to describe the effects of short term Schroth exercise on the Cobb angle, angle of trunk rotation (ATR), cosmetic appearance, and quality of life (QOL) in idiopathic scoliosis patients. METHODS: Five subjects with idiopathic scoliosis, (female, 3; male, 2) curvature type: thoracic, 2; lumbar, 3 underwent short term Schroth exercise for seven days. The exercise was performed for 3 h long sessions per day. The Cobb angle and QOL were measured before and after the intervention. ATR and cosmetic appearance were measured once. Sessions consisted of one baseline, seven intervention and three follow-up phases. The sessions were conducted with a one day interval. RESULTS: After the intervention, the Cobb angle was found to be significantly improve (p <.05), while the QOL did not differ (p>.05). The ATR showed decreased trends in the intervention phase. Data points during the intervention and follow-up phases showed a decrease in comparison with data points at the baseline, indicating that Schroth exercise might be effective in reducing the ATR and that these effects can be maintained after the intervention. Cosmetic appearance did not showed changed trends during the Schroth exercise intervention phase. CONCLUSION: Our results suggest that short term Schroth exercise may be valuable in improving the Cobb angle and ATR in patients with idiopathic scoliosis.

• 생명과학회지
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• 제26권6호
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• pp.721-726
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• 2016

누에(Bombyx mori)에서 short neuropeptide F (sNPF) receptor를 encoding하고 있는 cDNA를 cloning하여서 BsNPF-R라고 이름을 붙였다. BsNPF-R는 이미 보고된 sNPF-R들과 아미노산 수준에서 사람(36%), 쥐(34%), 제브라피쉬(35%), 초파리(51%)와 상동성을 보였다. BsNPF-R는 계산적으로 분자량이 42,731 Da이고 원형질막을 관통하는 단백질이다. BsNPF-R 유전자발현은 중장, 후견사선, 말피기관, 정소에서 강한 반면 지방체, 혈세포, 난소에서 약하게 발현하였다. 그리고 합성된 sNPF에 의해서도 BsNPF-R의 유전자발현이 조절되었다.

• 한국식품위생안전성학회:학술대회논문집
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• 한국식품위생안전성학회 2001년도 The Asia-Pacific Conference on Reproductive Biology and Environmental Sciences
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• pp.232-234
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• 2001

• International Journal of Contents
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• 제8권3호
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• pp.52-56
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• 2012

This study examines whether the infant mortality rate and life expectancy at birth are affected by health care expenditure in Korea. It can be provisionally concluded that the infant mortality rate tends to be affected by the health system itself in the long-run, whereas life expectancy at birth is immediately affected by health-related facilities such as the number of physicians and number of hospital beds in the short-run. Therefore, the health-related system should be well established to improve the infant mortality rate. On the contrary, physical capital such as life-prolonging medical technologies has to be accumulated to improve life expectancy at birth.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2004년도 춘계 학술발표대회 개요집
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• pp.252-254
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• 2004

Fatigue life a box weldment was predicted with the stress concentration and residual stress using the equation reported. In order to change the stress concentration and residual stress of the box weldment, Post treatments such as smooth grinding of weld bead, weld toe grinding and hammer peening were applied. The fatigue life of the weldment after post treatment clearly increased, which is attributed to the reduction of stress concentration and/or introduction of compressive residual stress at the weld toe. The predicted fatigue life was a relatively good agreement with the experiment for a long fatigue life, while it was underestimated for a short fatigue life.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1303-1308
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• 2008

In this study, we researched how to estimate life-stress relationship and acceleration factor through performing accelerated life test and analyzing it. The purpose of this study is to predict life of pneumatic cylinder within short time which is widely used in automation manufacturing line. In design of accelerated life test, we selected operating pressure and load that have the most influence on main failure mode of pneumatic cylinder as accelerated factor. We used two-way factorial design for arranging of test condition to accelerated factor and accelerated level.

• 한국신뢰성학회지:신뢰성응용연구
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• 제2권1호
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• pp.15-22
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• 2002

This paper is concerned about an Accelerated Life Test for Micro Display Device which is being used in a Projection TV, in order to find a failure mode occurred in field in a short time, to identify a major factor to affect a life, and to estimate a mean life. For this purpose, we selected a temperature as a accelerated factor to perform a test and measured degradation of display device using visual inspection and chromaticity table. In the result of Accelerated Life Test, it is confirmed that failure mode is equal to the degradation of display device by vendor and the Temperature is a major factor to affect a failure. Besides, according as the display device is turned to green as degraded, it is identified that the change of the chromaticity value is one method to measure the degree of the degradation . So, we applied the optimal condition, which consider a cost and life to lower the Temperature which is a major factor acquired by the result of ALT, to PTV design

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1997년도 추계학술대회 논문집
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• pp.360-363
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• 1997

The method of estimating life time of epoxy composites which be widely used for transformers has been studied in this paper. The breakdown properties of specimens are observed by appling high AC voltage at the room temperature from a series of the experiments. Afterwards, the breakdown time was determined under the constant voltage below the lowest breakdown voltage. Also, V-t properties were found out using weibull distribution widely used in the applications of discrete data for estimating life time of epoxy composites and life exponent n was gained properly. when life exponent is gained is found out, the tong breakdown life time at used voltage can be estimated from breakdown experiments in short time using reverse law of n power.