Nine plant foods (persimmon leaf, perilla seed, Chinese quince, ginger root, walnut, mugwort leaf, arrowroot, buckwheat and sorghum) rich in phenolic substances were examined for their effects on the digestive enzymes, food-poisoning bacteria and mutagenicity/antimutagenicity by Ames test. Among tested samples, Chinese quince significantly inhibited the $\alpha-amylase$ activity (97%), exhibiting an uncompetitive inhibition type. Protease activity was inhibited by Chinese quince (86%), persimmon leaf (51%) and mugwort leaf (20%), in which mugwort extract exhibited a noncompetitive type. Lipase was activated >50% by all samples. The inhibition of $\alpha-amylase$ was highly correlated with the content of condensed tannin (r=0.89) and the inhibition of protease, with total phenolic content (r=0.84). Total phenolies fraction of tested samples showed the growth inhibition toward E. coli. Streptococcus faecalis and Salmonella enteritidis, in which the effect of perilla, sorghum and arrowroot was the highest for E. coli. Standard phenolics and food samples did not show any mutagenicity toward Salmonella typhimurium TA98 and TA100. Tannic acid inhibited the mutation of the two strains by benzo[a]pyrene whereas total phenolics fractions of Chinese quince and walnut exhibited antimutagenicity to a lesser extent.
Kim, Tae-Keun;Kim, Hyoun-Chol;Song, Jin-Young;Ha, Young-Sam;Kang, Jeong-Hwan;Woo, Seong-Bae;Song, Chang-Khil;Son, ,Chang-Khi
Korean Journal of Organic Agriculture
/
v.19
no.1
/
pp.65-82
/
2011
In order to evaluate the weed suppressing effect of Solanum viarum Dunal. In this study species diversity in patch of S. viarum and allelopathic effects of the aqueous extracts on S. viarum were investigated. Number of species and species diversity in site close to patch of S. viarum were decreased gradually 1site ($7.7{\pm}2.0,\;1.5{\pm}0.2$), 2site ($5.3{\pm}1.2,\;1.2{\pm}0.2$) and 3site ($4.0{\pm}1.7,\;0.9{\pm}0.1$). And total phenolic compounds of soil in survey area were increased gradually site1 ($0.16{\pm}0.01mg\;g^{-1}$), site2 ($0.17{\pm}0.01mg\;g^{-1}$) and site3 ($0.22{\pm}0.02mg\;g^{-1}$). So the number of species and species diversity (r=-0.692, P<0.05) were negatively correlated with increased total phenolic compounds of soil in the survey area. The relative germination ratio, the mean germination time, the relative elongation ratio, the fresh weight and the dry weight of receptor plants are generally getting decreased while the concentration of the aqueous extracts from S. viarum escalate. But every ratio was various depending on the growing regions, the kind of receptor plants and the treatment of the aqueous extracts. Especially, the radicle by injection of the aqueous extracts concentration of S. viarum was influenced more than the shoot on the same condition. The total phenolic compounds on region of S. viarum was gradually increased in stems (fresh $0.56{\pm}0.02mg\;g^{-1}$, dry $1.58{\pm}0.08mg\;g^{-1}$), roots (fresh $1.77{\pm}0.07mg\;g^{-1}$, dry $2.64{\pm}0.06mg\;g^{-1}$), leaves (fresh $6.01{\pm}0.14mg\;g^{-1}$, dry $7.04{\pm}0.29mg\;g^{-1}$), seeds (fresh $6.21{\pm}0.17mg\;g^{-1}$, dry $9.08{\pm}0.73mg\;g^{-1}$) in order. On the contrary, the negative correlation on germination and growth of receptor plants was shown by total phenolic compounds on the each parts of S. viarum. We think that the aqueous extracts of S. viarum showed allelopathic effects on other plants. Therefore, S. viarum holds the higher competitiveness in plant community in Jeju Island and makes possibility of application as natural herbicide.
Park, In Hyeop;Park, Min Su;Lee, Kyeong Hak;Son, Yeong Mo;Seo, Jeong Ho;Son, Yowhan;Lee, Young Jin
Journal of Korean Society of Forest Science
/
v.94
no.6
/
pp.441-445
/
2005
Researches on estimating national-scaled forest biomass are being carried out to quantify the carbon stock of forests with the Kyoto Protocol. In general, estimates of national-scaled forest biomass are based on forest inventory data which provides estimates of forest area, stem volume, and growth of stem by age classes. Estimates of forest biomass are, however, obtained by converting stem volumes to dry weight with stem density and thereafter to whole tree biomass with biomass expansion factors (ratios of whole tree dry weight to stem dry weight). Pinus densiflora is widely distributed and one of the most economically important timber species in Korea. The species are largely grouped into two ecotypes of Geumgang and Jungbu. Stems of Geumgang type trees are straight and high compared to those of Jungbu type trees. The objective of this study was to determine and compare stem density and biomass expansion factors fore two ecotypes of Pinus densiflora according to stand age. Stem density of both ecotypes of Pinus densora increased and biomass expansion factors of them decreased with increasing tree age. In he same age class, stem density and biomass expansion factor of Geungang type Pinus densiflora were lower than those of Jungbu type Pinus densiflora. There were statistically significant differences in stem density and biomass expansion factors between Geumgang type and Jungbu type Pinus densiflora in 0-20-year-old stands and 40-60-year-old stands. Our results suggested that the reliability of the national forest biomass inventory could be improved by applying the ecotype- and age-dependent stem density and biomass expansion factors.
In order to investigate the difference of rootability between 15 clones of Populus alba${\times}$glandulosa selected based on the growth performance, rooting of cutting experiments with these 15 clones were conducted at the nursery for six years from 1970 to 1975. Cutting experiments in a temperature controlled incubator in which the temperature of the cutting bed were set to $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$ and $30^{\circ}C$ were also performed. Along with these experiments air layering experiments were performed to compare with the rootabilities obtained from nursery trial. The results obtained so far could be summarized as follows. 1. The best rooting clones were 65-22-4 and 65-22-11, and the average rooting percentages of these two clones for six years were 76.7%, and 72.9% respectively. The poorest rooting clone was 66-6-8 showing average rooting percentage of 45.8%. 2. The middle class of rooting percentage was ocuppied by the clones; 66-14-29, 66-14-93, 66-25-5 and 67-6-3, and the range of their rooting percentage was 60~69% on average. 3. The rooting performances observed through the nursery, the incubator and the air layering experiments were almost the same with exception of few clones. 4. P. alba${\times}$glandulosa showed the best rooting percentage at the cutting bed of $20^{\circ}C$ 5. The most roots, i.e. 78.5% of root per cutting were developed from the bottom part of the cutting shoot. 6. Adventitious and call use roots could observe in the cuttings.
This study was conducted to investigate host plant, bionomics, and damage on Bamboo Leaf Rollers in Chonnam area. Species of the leaf rollers attacking the bamboo trees in Chonnam area were Microstega jessica (Butler) and Sinibotys evenoralis (Walker). Among these species, M. jessica (B.) and S. evenoralis (W.) were observed in Damyang but S. evenoralis (W.) alone was found in Naju and Hwasoon. Among the 4 host plant species observed, Phyllostachys nigra var. henonis S., and P. nigra M. were first recorded as host plants of M. jessica (B.), and P. nigra var. henonis (S.) as host plant of S. evenoralis (W.). The occurrence of the M. jessica (B). was once a year with the peak about late May, whereas the occurrence of S. evenoralis (W.) was 2 times a year and the peaks were early June and mid-July, respectively. The average periods of each stage of M. jessica (B.) were 8 days for egg, 293 days for larva, 16 days for pupa, and the longevity of the adult was 12 days for females and 9 days for males. The average periods of each stage of S. evenoralis (W.) were in 1st generation, 11 days for egg, 28 days for larva, 12 days for pupa, and the longevity of the adults was 18 days for females and 15 days for males; and 2nd generation was 7 days for egg, 261 days for larva, 20 days for pupa, and the longevity of the adults was 11 days for females and 10 days for males. The time of most adult emergence of M. jessica (B.) was from 6 p.m. to 4 a.m. and the peak was 8 p.m. to 10 p.m. M. jessica (B,) overwintered as 6th instals in Bamboo shoot sheath but S. evenoralis (W.) overwintered as $1st{\sim}2nd$ instals on the tree leaves of Bamboo. The percentage of damaged leaves with different heights of Bamboo trees were 67.1% at upper portion, 19.0% at middle portion, 14.0% at lower portion. The percentage of damaged loaves with different Bamboo species was 57.0% on P. nigra var. henonis S., 36.5% on P. bambusoides S. et Z., 14.5% on P. pubescens M.. It was showed highly significant negative correlation between percentage of damaged bamboo leaves an tree growth stages.($r=-0.739^{**}$)
Inappropriate storage of fresh-cut onions may result in losses of good quality. To understand storage conditions for shelf-life and quality of fresh-cut onions, The effect of packing type and storage temperature on the quality of fresh-cut onions was evaluated. Onions stored at $0^{\circ}C$ for 2 months were peeled off after removing root and shoot parts. Each three peeled onions were packed in a polyethylene film (PE, $50{\mu}m$) or in a polyethylene/polypropylene film (PE/PP, $100{\mu}m$) with vacuum treatment (70 cmHg) and stored at different temperatures (4, and $10^{\circ}C$) for 21 days. The following analyses were examined to evaluate the quality of fresh-cut onions: microbial population, surface color, titratable acidity and pH, respiration rate, and sensory quality. Fresh-cut onions stored at $4^{\circ}C$ showed less aerobic and coliform bacterial population than those stored at $10^{\circ}C$ during observation periods. Fungal populations of fresh-cut onions packed in PE film stored at $10^{\circ}C$ increased significantly after 13 days. E. coli was not detected in all treatments during whole storage periods. Surface colors of fresh-cut onions were not affected by packing type and storage temperature, however, color difference (${\Delta}E$) of fresh-cut onions in PE/PP film stored at $10^{\circ}C$ was significantly higher than those of other treatments. Titratable acidity of fresh-cut onions was not affected by packing type and storage temperature. However, pH of fresh-cut onions packed in PE film stored at $10^{\circ}C$ increased gradually over the whole storage period. Fresh-cut onions packed in PE film showed higher $CO_2$ and less $O_2$ concentrations at $10^{\circ}C$ than those at $4^{\circ}C$. The sensory quality of fresh-cut onions was significantly affected by packing type and storage temperature after 13 days. Particularly, vacuum treatment in PE/PP film showed better sensory quality than that of PE film package at the same storage temperature. It was concluded that vacuum treatment and storage at $4^{\circ}C$ could be effective to prolong the quality of fresh-cut onions up to 21 days.
A severe defoliation by typhoon in early autumn reduces fruit quality for the current season and reserve accumulations for the next season. This study was conducted to determine the effects of foliar applications during the autumn on alleviating the damages after defoliation. Leaves of 2-year-old 'Fuyu' trees, grown in 50-L pots under a rain-shelter, were 75%-defoliated on September 9. In mid-September and early October, trees were treated either with eight foliar applications of urea or with four alternating applications of urea and $KH_2PO_4$ (urea + KP application), all at 0.5% (w/v). Trees untreated after the defoliation served as the control. The urea applications slightly increased N and P concentrations of the leaves collected on November 6, while urea + KP applications significantly increased P and K concentrations. Foliar applications did not affect fruit growth, but tended to decrease skin coloration. Fruit soluble solids increased by 1.5 and $1.0^{\circ}Brix$ for urea and urea + KP applications, respectively. There was a significant increase in dry weight of fine root for the foliar application treatments but not in those of aerial woods and larger roots. With the foliar applications, N concentration tended to increase in the permanent organs but not P and K, whereas soluble sugars and starch notably increased in shoot, trunk, or fine root regardless of the different applications. Results indicated that the foliar applications could partially help to restore fruit quality and carbohydrate accumulations in the defoliated trees.
A water culture experiment was conducted to investigate the effect of As content in a culture solution on the water status and growth of rice plants. Rice (Oryza sativa L. Line. Iri 316) seeds were germinated in bentonite and cultivated there for 30 days. Rice seedlings were transplanted into 3.5l pots containing the culture solution on May 1, 1985 and allowed to grow without As treatment for one month. Afterwards, they were grown in a culture solution maintaining the final concentration of As, 0, 1, 5, 10 and 15mg/1 renewing in the solution dissolved sodium arsenate at intervals of 3 to 7 days. Plants were cultivated in the green house during the growing period and harvested 60 days after As treatment. The results obtained were as follows: Transpiration of rice plants was decreased with the increase of the As level in the culture solution. Stomatal diffusive resistance and leaf temperature increased with increase of As levels though the humidity and the air flow rate in leaf decreased. Air flow rate, transpiration and stomatal diffusive resistance showed a highly significant correlation with As contents in shoots and roots of rice plants: Espally The air flow rate and transpiration revealed a significantly higher correlation with As contents in the root than that in the shoot, but diffusive resistance showed adverse tendency. High levels of As in the culture solution depressed plant height, no. of tillers, leaf width and dry weight of plant remarkably. Typical symptoms of As toxicity were root discoloration, and necrosis of leaf tips and margins, and leaf rolling during the sunny daytime were also another symptoms.
The present study examined the epidermal changes of adhesive disks which occur during attachment in Parthenocissus tricuspidata using scanning and transmission electron microscopy. Several adhesive disks, each covered with a bract, develop from the shoot apical meristem during early development. In the initial stage, the adhesive disks are club-shaped and their upper and lower epidermis are indistinguishable. However, in the actively growing stage, they become spherical and both epidermis are clearly differentiated into the adventitious roots. Prior to wall attachment, the adhesive disks exhibit adaxial convex and abaxial concave shapes, and electron-dense substances are abundant in the vacuoles of epidermal cells. The peripheral area of the adhesive disk is adhered first to the wall surface, while the central area is drawn inward in a vacuum-like state during attachment. As the attachment progresses and the electron-dense substances continue to discharge, the upper and lower epidermis rapidly undergo deterioration and the disks shrink considerably. At this stage, structural changes of the lower epidermis occur much faster than in the upper one. The discharged substance is accumulated on the wall surface, and this aids the attachment of adhesive disks on the wall for long periods. In this manner, the shape and structure of the adhesive disk epidermis change drastically from initial growth to the mature stage. Further, the role of electron-dense substance and shrinkage of the disk during attachment has been discussed in Parthenocissus tricuspidata.
A high sugar rice cultivar 'Danmi' which has been recently developed and released in the Functional Crop Resource Development Division, Department of Functional Crop, National Institute of Crop Science, has high glucose and sucrose contents but has a poor seed development. This defect poses some problem in ensuring stable seedling establishment in the nursery bed. We examined the germination and seedling emergence characteristics of 'Danmi' and determined an optimum seeding rate for seedling establishment. 'Danmi' seeds has a light 1,000-grain weight of 18.2g compared with Ilmibyeo's 26.7g. The 97.4% of 'Danmi' seeds has specific gravity below 1.0 while only 2.0% of seeds was fully developed with specific gravity above 1.12. However, the reverse is true in the Ilmibyeo seed used as a control cultivar. Although the seed germination of Danmi was 89-91% regardless of seed specific gravity but at a slower rate than Ilmibyeo regardless of water soaking temperatures of $20-30^{\circ}C$ because of high seed amount of specific gravity below 1.0 in 'Danmi'. It took 2~4 days longer of seed soaking in the Danmi seeds than Ilmibyeo before germination. 'Danmi' has low normal seedling emergence rate than Ilmibyeo in the seedbed soil due to high percentage of abnormal seedlings like stunting and incomplete growth. Normal seedling emergence rate of 'Danmi' in the seedbed was 62.6~64.9% for 10-day old seedling and 83.5~86.7% for 30-day old seedling which is lower by 29.6~30.0% and 11.3~12%, respectively than Ilmibyeo. Although 'Danmi' has low normal seedling emergence rate, but it has greater seed number per weight basis. Therefore, based on the normal seedling number per unit area of Ilmibyeo(control cultivar) for seedling rate of 10-day and 30-day old seedlings, the recommended seeding rate of 'Danmi' for transplanting rice is 220g seeds for 10-day old seedling and 130g for 30-day old seedling per nursery box, which is equivalent to 1.5 times seed volumes of Ilmibyeo.
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