• Journal of Life Science
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• v.26 no.12
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• pp.1360-1366
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• 2016

Heat shock proteins (HSPs) are induced in response to various physiological or environmental stressors. However, the transcriptional activation of HSPs is regulated by a family of heat shock factors (HSFs). Fish models provide an ideal system for examining the biochemical and molecular mechanisms of adaptation to various temperatures and water environments. In this study, we examined the pattern differentials of heat shock factor 1 (HSF1) and expression of heat shock protein 70 (HSP70) in response to thermal stress in goldfish and mouse hepatocyte cultures by immune-blot analysis. Goldfish HSF1 (gfHSF1) changed from a monomer to a trimer at $33^{\circ}C$ and showed slightly at $37^{\circ}C$, whereas mouse HSF1 (mHSF1) did so at $42^{\circ}C$. This experiment showed similar results to a previous study, indicating that gfHSF1 and mHSF1 play different temperature in the stress response. We also examined the activation conditions of the purified recombinant proteins in human HSF1 (hmHSF1) and gfHSF1 using CD spectroscopy and immune-blot analysis. The purified recombinant HSF1s were treated from $25^{\circ}C$ to $42^{\circ}C$. Structural changes were observed in hmHSF1 and gfHSF1 according to the heat-treatment conditions. These results revealed that both mammal HSF1 (human and mouse HSF1) and fish HSF1 exhibited temperature-dependent changes; however, their optimal activation temperatures differed.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2010.10a
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• pp.503-504
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• 2010

• International Journal of Aeronautical and Space Sciences
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• v.11 no.1
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• pp.49-57
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• 2010

Several pyrotechnic devices are employed over the course of satellite's missions, generally for the separation of structural subsystems and deployment of appendages. Firing of pyrotechnic devices results in impulsive loads characterized by high peak acceleration and high frequency content which can cause failures of various flight hardware elements and small components. Thus, accurate prediction of acceleration level in various components of spacecraft due to pyrotechnic devices is important. In this paper, two methods for pyroshock prediction, an empirical model and statistical energy analysis in conjunction with virtual mode synthesis, are applied to predict shock response of a low altitude earth observation satellite during launch vehicle separation. The predicted results are then evaluated through comparison with the shock test results.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.14 no.7
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• pp.551-560
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• 2004

A method how to determine the shock response spectrum from the FRF of the statistical energy analysis( SEA ) is presented here. The system of 3 different Plates connected by bolt joints is selected simulating missile structural sections Joined together. First, the SEA model was rendered by SEA parameters which were determined from experimental SEA method. Then, the mobility power was input to the SEA model and we can verify the validity of the model in the medium to high frequency range checking the reproduction of output average velocity. And, the shock induced shock response spectrum(SRS) was obtained using SEA FRF and arbitrarily chosen experimental FRF. We have compared the thus obtained SRS with actually measured SRS and they were relatively in good agreement. In this paper, we used the measured SEA FRF and therefore we have got the SRS well agreed with actually measured SHS even in the low frequency range. If the SEA FRF of well verified SEA model is used, the good result will come out in SEA effective frequency range which is more important at SRS.

• Biomedical Science Letters
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• v.9 no.2
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• pp.59-65
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• 2003

When cells are exposed to proteotoxic stresses such as heat shock, amino acid analogs, and heavy metals, they increase the synthesis of the heat shock proteins (HSPs) by activating the heat shock transcription factor 1 (HSF1), whose activity is controlled via multiple steps including homotrimerization, nuclear translocation, DNA binding, and hyperphosphorylation. Under unstressed conditions, the HSF1 activity is repressed through its constitutive phosphorylation by glycogen synthase kinase 3$\beta$ (GSK3$\beta$), extracellular regulated kinase 1/2 (ERK1/2), and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK). However, the protein kinase (s) responsible for HSF1 hyperphosphorylation and activation is not yet identified. In the present study, we observed that profile of p38 mitogen-activated protein kinase (p38MAPK) activation in response to heat shock was very similar to those of HSF1 hyperphosphorylation and nuclear translocation. Therefore, we investigated whether p38MAPK is involved in the heat shock-induced HSF1 activation and HSP expression. Here we show that the p38MAPK inhibitors, SB202190 and SB203580, but not other inhibitors including the MEK1/2 inhibitor PD98059 and the PI3-K inhibitor LY294002 and wortmannin, suppress HSF1 hyperphosphorylation in response to heat shock and L-azetidine 2-carboxylic acid (Azc), but not to heavy metals. Furthermore, heat shock-induced HSF1-DNA binding and HSP72 expression was specifically prevented by the p38MAPK inhibitors, but not by the MEK1/2 inhibitor and the PI3-K inhibitors. These results suggest that SB202190- and SB203580-sensitive p38MAPK may positively regulate HSP gene regulation in response to heat shock and amino acid analogs.

• Journal of the Korea Institute of Military Science and Technology
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• v.24 no.1
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• pp.70-78
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• 2021

Naval surface ships and submarines could be exposed to non-contact underwater explosion(UNDEX) environment. Equipment installed on the ships and submarines could be damaged by shock load generated by UNDEX environment. Therefore, shock survivability of equipment generally evaluated by shock tests. Ground based shock test machine such as Light weight shock test machine(LVSM) is developed to simulate shock load caused by UNDEX environment. In this study, linear dynamic model of LVSM is proposed and evaluated to improve shock test design procedure. Parameters of the model are decided by optimizing time domain response compared to zero payload experiment. Proposed model is verified by comparing simulation results and test results of maximum payload experiment. Finally, shock test design using the model is described for various test equipment weight.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.22 no.1
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• pp.81-87
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• 2012

This paper establishes a modeling and simulation procedure for structural response and reliability of a cylindrical array sensor on submarines under the shock generated by underwater explosion. The structural reliability of SONAR is important because the submarine could get out of combat ability by the structural damage of the SONAR upon explosion. A cylindrical array sensor was first modeled using the finite element method. Modal analysis was then performed for the check of the reliability of the modeling. The shock resistance simulations were performed for the responses to the structural shock waves and for the responses to the directly applied underwater shock waves, according to BV-043 and MIL-STD-901D, respectively. The stresses of the structure were evaluated with von-Mises scheme. Vulnerable regions were exposed through mapping the maximum stress to the structural model. Maximum stress of the SONAR was compared with the yield stress of the material to examine the structural reliability.

• Biomedical Science Letters
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• v.12 no.4
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• pp.435-438
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• 2006

In eukaryotes, ER stress induces UPR (unfolded protein response) via IRE1 activation which sends a molecular signal for XBP1 mRNA splicing in the cytosol. During this mRNA splicing, 23 nt removed in which contains PstI site and then resulting XBP1 product is not digested with PstI restriction enzyme. In this study, using this XBP1 mRNA splicing mechanism, the effect of heat shock on thyrocytes is studied, because heat shock response in the thyrocytes needs more study to understand thyroid physiology under alternative environments. ER inducible drugs (tunicamycin, DTT, $Ca^{2+}$ ionopore A23187, BFA) induce ER stress in the thyrocytes. From 3 hours after heat shock, ER stress is induced and which is reversible when heat shock is without. While $Ca^{2+}$ ionopore A23187 is reversible from ER stress by washing out the drug, thapsigagin is irreversible. Other ER inducible drugs are not so sensitive to ER stress repairing. XBP1 mRNA splicing in a cell is very available method to detect ER stress. It needs only a small quantity of total RNA and processing also very easy.

• Advances in aircraft and spacecraft science
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• v.6 no.6
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• pp.497-513
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• 2019

A wind tunnel test was conducted that measured surface fluctuating pressures aft of a ramp at transonic speeds. Dynamic pressure test data was used to perform a study to determine best locations for streamwise sensor pairs for shocked and unshocked runs based on minimizing the error in root-mean-square acceleration response of the panel. For unshocked conditions, the upstream sensor is best placed at least 6.5 ramp heights downstream of the ramp, and the downstream sensor should be within 2 ramp heights from the upstream sensor. For shocked conditions, the upstream sensor should be between 1 and 7 ramp heights downstream of the shock, with the downstream sensor 2 to 3 ramp heights of the upstream sensor. The shock was found to prevent the passage coherent flow structures; therefore, it may be desired to use the shock to define the boundary of subzones for the purpose of loads definition. These recommendations should be generally applicable to a range of expansion corner geometries in transonic flow provided similar flow structures exist. The recommendations for shocked runs is more limited, relying on data from a single dataset with the shock located near the forward end of the region of interest.

• Journal of the Society of Naval Architects of Korea
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• v.42 no.5 s.143
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• pp.499-505
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• 2005

Underwater explosion shock response analysis of a nonlinear double resiliently mounted equipment on a MIL-S-901D Large floating Shock Platform(LFSP) was carried out using LS-DYNA3D/USA. As a nonlinear double resiliently mounted equipment, real main engine module of naval ship was considered, where the engine, bearing, and base frame including sound enclosure were treated as rigid bodies with six degrees of freedom. The nonlinear effects of resilient mounts on its shock response characteristics were examined, and the usefulness of our suggested method was also confirmed comparing with calculation results by the equipment maker.