• KSBB Journal
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• 제14권6호
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• pp.702-706
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• 1999

대표적인 양식 해조류인 방사무늬 김 엽체를 대상으로 하여 산 처리에 의한 유전형질의 표현 변화를 differential display기법으로 비교하여 보았다. 방사무늬 김 엽체를 0.05% HCl을 첨가한 해수(pH 3.0)에서 5분간 처리한 후 각각 10분, 30분, 60분 그리고 4시간동안 멸균 해수에서 정치 배양시키면서, RNA를 추출하여 cDNA합성, PCR 증폭, agarose gel 전기영동 및 DNA 염기배열을 조사하였다. 그 결과 arbitrary primer OPA 1(CAGGCCCTTC)을 사용하여 differential display한 경우, 산 처리 후 30분간 멸균 해수에서 정치 배양한 엽체에서 특이적으로 RNA 합성이 일어나지 않은 유전자를 분리할 수 있었으며, 그 염기서열을 비교한바 이 유전자 fragment(605 bp)는 dethiobiotin synthetase 유전자와 93%의 높은 상동성을 가진 것으로 나타났다.

• Journal of Microbiology
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• 제35권4호
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• pp.271-276
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• 1997

We isolated phenotypic suppressors of an absB (antibiotics synthesis suppression) strain. In the absB colonies, all four antibiotics including two pigmented antibiotics were blocked so that no pigmentation could be found. We assumed that in the colonies with the wuppressive(or reversive) mutation, both pigmentation would be restored so that the strains with suppressive mutation could be cisually detected. Harvested absB spores were treated with chemical mutagen along with electric shock, and were spread on specially fromulated minimal medium plates. The pigmented colonies were isolated from the unpigmented majorities. In one candidate strain, the restoration and significant overproduction of actinorhodin and undecylprodigiosin were recognized. In three other candidate strains, the overproduction of actinorhodin and restoraion of undecylprodigiosin were observed. The production of the two unpigmented antibiotics (CDA and methylenomycin) were visualized in the tested candidate strains. The strains with wuppressive mutations would be very useful in dlucidating the regulation network of antiviotics synthesis and overproduction of the antibiotics.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 춘계학술대회 논문집 전기설비전문위원
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• pp.80-83
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• 2005

우리나라의 실내의 배전로는 접지방식(Grounded System)이 일반적이고, 전로의 일선 지락 시에는 누전전류 차단기 혹은 과전류 차단기에 의해 전로를 차단하여 전기적인 사고 예방을 도모하고 있다. 그러나, 생명 유지 장치(Life-Sustaining Equipment)를 사용하고 있는 수술실 등 의료실의 배전 계통에 있어서의 전로의 차단은 치명적인 사고를 유발 할 수 있다. 이에 비하여, 비접지 배선방식(Isolated Power System)은 일선 지락 시에도 지락 전류 ((Ground-Fault Current)를 현저히 적게 할 수 있기 때문에 전원 공급은 계속되어 질 수 있다. 그러므로, 전원 공급의 높은 신뢰성이 요구되는 의료용 전기기기의 회로에는 비접지배선 방식으로 시설토록 규정 하고 있다. 또한, 지락 전류가 현저히 적기 때문에 선로 절연 감지기(Line Isolation Monitor)를 설치하여 의료진이나, 환자를 누설 전류에 의한 전기적 쇼크(Electrical Shock)를 방지하는 시스템이다.

• 미생물학회지
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• 제33권1호
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• pp.38-42
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• 1997

자연계로부터 erythritol 생산균주를 선발하기 위하여 옥수수밭이나 사탕수수밭의 토양이나 자판기와 같은 당이 많은 지역으로부터 sampling하여 검색한 결과 erythritol을 생산하는 약 200여 균주를 분리할 수 있었으며 이들을 paper chromatography법으로 1차 선별한 후 HPLC를 이용하여 배양액 중의 erythritol 생산량을 측정함으로서 생산성이 가장 우수하고 부생성물이 가장 적은 KJ81균주를 최종 선발하였다. 선발된 균주의 형태학적 생리학적 특성을 조사하여 penicillium sp. KJ81로 동정하였다. 균주의 콜로니 색은 백색에서 녹색으로 변화하였고 1-3단계 분기의 분생자병과 플라스크형의 경자를 보였다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1478-1481
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• 2012

Geldanamycin (GM) and its analogs are important anticancer agents that inhibit heat shock protein (Hsp) 90, which is a major chaperone protein in cancer cells. Accordingly, based on interest in obtaining novel natural GM derivatives, the potential of Streptomyces hygroscopicus JCM4427, a GM producer, was explored for novel natural GM derivative(s), resulting in the discovery of new GM analogs as a biosynthetic shunt product and intermediates from its fermentation broth. In this study, the fermentation, isolation, structure determination, and biological activity of the compounds, two new tetracyclic thiazinogeldanamycin (1) and 19-hydroxy-4,5-dihydrogeldanamycin (3), together with the three known 4,5-dihydrothiazinogeldanamycin (2), reblastatin (4), and 17-demethoxy-reblastatin (5), are described.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.320.3-321
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• 2002

Nuclear factor ${\kappa}$B (NF-${\kappa}$B) represents a family of eukaryotic transcription factors participating in the regulation of various cellular genes. Since aberrant regulation of NF-${\kappa}$B has been implicated in the pathogenesis of various diseases including inflammation. asthma. atherosclerosis. AIDS. septic shock. arthritis, and cancer. this transcription factor has been shown to be an interesting target of new drug discovery. (omitted)

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 춘계학술대회논문집
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• pp.131-136
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• 2004

Impact sounds, such as those created by footsteps, the dropping of an object or the moving of furniture, can be a source of great annoyance in residential buildings. The character and level of impact noise generated depends on the object striking the floor, on the basic structure of the floor, and on the floor covering. This study base on the evaluate of isolation performance of impact sound according to the impact noise reduction methods. Reduction methods consist of four ways. First way is increase thickness of bare floor and other ways are using the soft coverings on the floor and ceiling assembles. Last way is make floating floor with shock absorbing materials.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 춘계학술대회논문집
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• pp.1019-1023
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• 2006

Conventional methods for reducing vibration in engineering designs may be undesirable in conditions where size or weight must be minimized, or where complex vibration spectra exist. Fe-Mn Damping alloy with a combination of high damping capacity and good mechanical properties can provide attractive technical and economical solutions to problems involving seismic, shock and vibration isolation. We have studied the noise and vibration characteristic of Dampalloy and checked Dampalloy reduced noise about 3.9dB and vibration about 15.9 times as compared conventional material through laboratory research. With this result, we obtained a good possibility of material substitution about the bridge expansion joint

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2002년도 제27회 정기총회, 제40회 학술발표회
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• pp.22.2-23
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• 2002

• 동력기계공학회지
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• 제18권2호
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• pp.77-82
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• 2014

The air spring is widely used in various fields such as a suspension system and an anti-vibration system because the natural frequency is kept constant regardless of the change in the load, spring constant is easy to change, and, vibration and shock isolation performance are excellent. The purpose of this study is to derive a nonlinear governing equation of an air spring, to analyze the effect of the various parameters on the dynamic stiffness of the air spring, and, to suggest a more efficient design method of an air spring system. In order to do so, this study investigates the impact of all the parameters that could affect the dynamic stiffness of the air spring while changing the excitation amplitude and the frequency with a developed governing equation.