• Mass Spectrometry Letters
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• v.5 no.4
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• pp.110-114
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• 2014

The method of direct mass spectrometry profiling is reliable and reproducible for the rapid identification of clinical isolates of bacteria and fungi. This is the first study evaluating the approach of MALDI-TOF mass spectrometry profiling for rapid identification of carbapenemase-resistant enterobacteriaceae (CRE). Proof of concept was achieved by the discrimination of CRE using MALDI Biotyper MS based on the protein. This profiling appears promising by the visual observation of consistent unique peaks, albeit low intensity, that could be picked up from the mean spectra (MSP) method. The Biotyper MSP creation and identification methods needed to be optimized to provide significantly improved differences in scores to allow for subspecies identification with and without carbapenemases. These spectra were subjected to visual peak picking and in all cases; there were pertinent differences in the presence or absence of potential biomarker peaks to differentiate isolates. We also evaluated this method for potential discrimination between different carbapenemases bacteria, utilizing the same strategy. Based on our data and pending further investigation in other CREs, MALDI-TOF MS has potential as a diagnostic tool for the rapid identification of even closely related carbapenemases but would require a paradigm shift in which Biotyper suppliers enable more flexible software control of mass spectral profiling methods.

• Journal of Digital Convergence
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• v.14 no.9
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• pp.369-378
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• 2016

The purpose of this study was conducted to determine the working conditions, post-traumatic stress syndrome and the self-resilience of nurses, and find out the relationship between the post-traumatic stress syndrome and the self-resilience. Data were collected by questionnaire method among 220 General Hospital nurses at Gyeongnam G and J city. Data were analyzed by using SPSS 21.0 program WIN. The results of working conditions of the nurses majority were shift work 70.5%, currently working in the ward 54.6%. Scores were shown as Post-traumatic stress syndrome (28.20) and self resilience (39.98). There is a negative correlation (r =.226, p=.001) between the post-traumatic stress syndrome and the self-resilience. Based on the result, since working conditions of nurses caused psychological stress and nurses with high self-resilience eased the degree of post-traumatic stress, in terms of the personnel management of hospitals, a psychological intervention strategy should be considered.

• Journal of Korea Society of Industrial Information Systems
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• v.12 no.5
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• pp.110-119
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• 2007

In an extended enterprise, there is a shift to shared services, cosourcing and outsourcing, and extending out to partners, suppliers, and customers to accomplish business objectives more effectively. Along with this critical need, executives should be aware of the need to focus on optimizing the value of their information technology and reducing the related risks. So IT governance is critical, and many companies including spin-off venture are providing IT governance solution, but very little is known about brand management and marketing strategy of IT governance solution provider. The purpose of this study is to investigate brand positioning of IT governance solution company focusing on spin-off venture. The results of this study are summarized as follows. First, brand management is needed in the spin-off venture. second, IT governance solution companies including spin-off venture must provide something more than functional value. That is, they actively seek to emotional or symbolic value for their customers.

• Journal of Electrical Engineering and Technology
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• v.13 no.1
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• pp.143-151
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• 2018

In this paper, a novel hybrid configuration combining a phase-shift full-bridge (PSFB) and a half-bridge resonant LLC converter is proposed for the On-Board Charger of Electric Vehicles (EVs). In the proposed converter, the PSFB converter shares the lagging-leg switches with half-bridge resonant converter to achieve the wide ZVS range for the switches and to improve the efficiency. The output voltage is modulated by the effective-duty-cycle of the PSFB converter. The proposed converter employs an active reset circuit composed of an active switch and a diode for the transformer which makes it possible to achieve zero circulating current and the soft switching characteristic of the primary switches and rectifier diodes regardless of the load, thereby making the converter highly efficient and eliminating the reverse recovery problem of the diodes. In addition an optimal power sharing strategy is proposed to meet the specification of the charger and to optimize the efficiency of the converter. The operation principle the proposed converter and design considerations for high efficiency are presented. A 6.6 kW prototype converter is fabricated and tested to evaluate its performance at different conditions. The peak efficiency achieved with the proposed converter is 97.7%.

• The Transactions of the Korean Institute of Power Electronics
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• v.8 no.6
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• pp.469-477
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• 2003

This paper proposes a Carrier-Rotation (CR) PWM technique that is a new solution for the voltage unbalancing problem of flying capacitors in the Flying Capacitor Multi-level Inverter (FCMI). The proposed technique equalizes the utilization of phase leg voltage redundancies corresponding to the charging and the discharging state of individual flying capacitors during each switching period of all the switches. Therefore, the charging and the discharging quantity of flying capacitors are equal, which makes the average variation of flying capacitor voltages become zero and keeps their voltage stable during minimum specified period. It also has the reduced harmonic contents of output voltage and the same switch utilization since all the carrier signals are in phase and the switching frequency of each switch is identical. The proposed technique is analyzed precisely in flying capacitor 3-level inverter and then it has expanded to the FCMI (N-level, N>3). Experimental results on the laboratory prototype flying capacitor 3-level inverter confirm the validity of the proposed technique.

• Mass Spectrometry Letters
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• v.5 no.3
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• pp.63-69
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• 2014

In this study, we present a new isotope-coded carbamidomethylation (iCCM)-based quantitative proteomics, as a complementary strategy for conventional isotope labeling strategies, with providing the simplicity, ease of use, and robustness. In iCCM-based quantification, two proteome samples can be separately isotope-labeled by means of covalently reaction of all cysteinyl residues in proteins with iodoacetamide (IAA) and its isotope (IAA-$^{13}C_2$, $D_2$), denoted as CM and iCCM, respectively, leading to a mass shift of all cysteinyl residues to be + 4 Da. To evaluate iCCM-based isotope labeling in proteomic quantification, 6 protein standards (i.e., bovine serum albumin, serotransferrin, lysozyme, beta-lactoglobulin, beta-galactosidase, and alpha-lactalbumin) isotopically labeled with IAA and its isotope, mixed equally, and followed by proteolytic digestion. The resulting CM-/iCCM-labeled peptide mixtures were analyzed using a nLC-ESI-FT orbitrap-MS/MS. From our experimental results, we found that the efficiency of iCCM-based quantification is more superior to that of mTRAQ, as a conventional nonisobaric labeling method, in which both of a number of identified peptides from 6 protein standards and the less quantitative variations in the relative abundance ratios of heavy-/light-labeled corresponding peptide pairs. Finally, we applied the developed iCCM-based quantitative method to lung cancer serum proteome in order to evaluate the potential in biomarker discovery study.

• Journal of the Korea Safety Management & Science
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• v.14 no.4
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• pp.229-238
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• 2012

Within last 10 years, internet has become a daily activity, and humankind had to face the Data Deluge, a dramatic increase of digital data (Economist 2012). Due to exponential increase in amount of digital data, large scale data has become a big issue and hence the term 'big data' appeared. There is no official agreement in quantitative and detailed definition of the 'big data', but the meaning is expanding to its value and efficacy. Big data not only has the standardized personal information (internal) like customer information, but also has complex data of external, atypical, social, and real time data. Big data's technology has the concept that covers wide range technology, including 'data achievement, save/manage, analysis, and application'. To define the connected technology of 'big data', there are Big Table, Cassandra, Hadoop, MapReduce, Hbase, and NoSQL, and for the sub-techniques, Text Mining, Opinion Mining, Social Network Analysis, Cluster Analysis are gaining attention. The three features that 'bid data' needs to have is about creating large amounts of individual elements (high-resolution) to variety of high-frequency data. Big data has three defining features of volume, variety, and velocity, which is called the '3V'. There is increase in complexity as the 4th feature, and as all 4features are satisfied, it becomes more suitable to a 'big data'. In this study, we have looked at various reasons why companies need to impose 'big data', ways of application, and advanced cases of domestic and foreign applications. To correspond effectively to 'big data' revolution, paradigm shift in areas of data production, distribution, and consumption is needed, and insight of unfolding and preparing future business by considering the unpredictable market of technology, industry environment, and flow of social demand is desperately needed.

• Journal of Korean Society of Archives and Records Management
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• v.19 no.1
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• pp.69-93
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• 2019

Archive exhibitions are one of the important tasks of archives that promote the value of archives and contribute to increasing users' interest and positive image but have not yet been activated. Recently, there has been a shift from a one-way archive exhibition to a new way to draw attention and understanding from users by using archival contents. This study aims to propose ways to activate archive exhibitions by utilizing archival contents by analyzing the exhibits of domestic cultural heritage institutions. Focusing on ways to improve the understanding and utilization of records through archival contents, the proposed strategy for developing archive exhibition programs is a three-way contents-based exhibition, experiential exhibition, and digital environment-based exhibition. The results of this study are expected to help develop archival exhibition programs that will enhance users' interest and understanding of records.

• Journal of The Korean Association of Information Education
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• v.22 no.6
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• pp.711-721
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• 2018

The development of artificial intelligence technology and the shift to a software-driven society are raising the need for digital literacy education on how to access, understand, use, create and share new open content in a variety of sustainable open content. At this point in time, this paper defines the digital literacy as the subliteracy concept for data, tools, and device elements. It is defined as a concept that includes cognitive and non-cognitive abilities and is stratified by computer literacy, ICT literacy, and information literacy. Open content is also defined as teaching-learning materials that can be used and shared freely by anyone, such as the Open Education Resource (OER) and the Open Access movement. Based on the two definitions, a three-step strategy for digital literacy education was developed to select open content in the digital environment, followed by a digital literacy education plan, and finally, an education frame to foster digital literacy capabilities.

• The Journal of the Korean dental association
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• v.57 no.8
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• pp.437-447
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• 2019

Purpose: In recent years, efforts to improve the dental curriculum in South Korea have focused on a shift to outcome-based dental education based on core competencies in dentistry. So far, the field has seen various studies on the development of competencies, performance evaluation, and the importance of outcome-based education, but few studies have documented the development of such an education model. Therefore, this study develops an OBE curriculum for dentistry education and describes the development procedures and then finally this study intends to share our experience to other dental schools. Methods: This study introduces the development procedure and details of an outcome-based education model for dental education and presents the five stages of an outcome-based education model. In this study, 3 educational experts and 2 dental professor composed the TFT and developed the research method according to the ADDIE model. Step 1 is to conduct quantitative / qualitative research analysis through some survey and interview, Step 2 is to do a survey to revise competency, Step 3 is to develop a materials through consensus and participation of our professors of the dental school, Step 4 is to do some workshops, Step 5 is to prepare and conduct a outcome evaluation. Results: Step 1 is a required process for developing an educational model: the Job Analysis & Need Analysis stage. Step 2 is the Development of Outcome and Competency stage, which involves revising the competencies that are the basis of the curriculum. Step 3 is developing competency descriptions, competency levels, and evaluation criteria?the Development of Outcomes and Evaluation Standards. Step 4 is the Development of Milestones for Curriculum and Instructional Strategy, which examines the curriculum's problems and analyzes the improvements of each course. Step 5 is the Evaluating Outcomes stage, conducted based on the competencies specified by the target dental school. Conclustion: The model presented here can serve as a foundation for outcome-based education in other dental schools.