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Latching Control Technology for Improvement of Extracted Power from Wave Energy Converter (파력발전기 추출파워 향상을 위한 래칭 제어기법)

  • Cho, Il Hyoung
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.18 no.4
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    • pp.282-290
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    • 2015
  • In this study, a latching control technology, proposed by Sheng et al.(2015), was applied in order to maximize the extraction efficiency of WEC (Wave Energy Converter), which is the heaving buoy coupled with linear electric generator. The latching control is the phase-control technique for improving the wave energy conversion with appropriate latching duration of keeping the buoy fixed. From the time-domain analysis in regular waves, the latching control technology can significantly improve the heave velocity and extracted power, even though the resonance condition is not satisfied. By using the latching control technology, the draft of buoy as well as the required PTO damping force can be significantly reduced along with increased extracted power, which is a big advantage in manufacturing the WEC.

Drug Resistance Effects of Ribosomal Protein L24 Overexpression in Hepatocellular Carcinoma HepG2 Cells

  • Guo, Yong-Li;Kong, Qing-Sheng;Liu, Hong-Sheng;Tan, Wen-Bin
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.22
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    • pp.9853-9857
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    • 2014
  • Background: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Materials and Methods: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null-transfected HepG2 cell lines. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.

Functional Characterization of 5-Enopyruvylshikimate-3-Phosphate Synthase from Alkaliphilus metalliredigens in Transgenic Arabidopsis

  • Xing, Xiao-Juan;Tian, Yong-Sheng;Peng, Ri-He;Xu, Jing;Zhao, Wei;Yao, Quan-Hong;Sun, Sheng
    • Journal of Microbiology and Biotechnology
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    • v.24 no.10
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    • pp.1421-1426
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    • 2014
  • Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based twostep DNA synthesis method to synthesize an aroA gene ($aroA_{A.\;metalliredigens}$) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new $aroA_{A.\;metalliredigens}$ gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.

Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene

  • Wang, Zhen;Yu, Hong-Sheng;Yao, Ru-Yong;Qiu, Wen-Sheng;Yue, Lu;Sui, Ai-Hua;Liu, Xiang-Ping;Liu, Shi-Hai
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.1
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    • pp.507-510
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    • 2013
  • Background: More and more research indicate that the immediately early response gene 3 (IER3) is involved inmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression. Methods: Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western-blotting. Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.

Fusulinids from the Carboniferous strata in the Gangdong area of Samcheok coalfield, Korea

  • Lee, Chang-Zin;Kim, Jun-Ho;Lee, Sang-Min
    • Journal of the Korean earth science society
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    • v.27 no.7
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    • pp.768-777
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    • 2006
  • The goal of this study is to elucidate the fusulinid biostratigraphy of the Carboniferous limestones distributed in the Gangdong area of Samcheok coalfield, Korea. The Carboniferous strata of the study area mainly comprise alternaton of dark gray shale, dark gray and reddish sandstone, and gray limestone. The limestones consist mainly of wackestonepackstone containing various fossil fragments such as crinoid, coral, brachiopod, foraminifera, fusulinid, and bryozoa; this observation thus suggests that the study site was the shallow marine environments. A tital of 12 species belonging to 5 genera of fusulinids are identified from the limestones of the Gangdong geologic section: Ozawainella turgida Sheng, Ozawainella sp. A, Ozawainella magna Sheng, Pseudostaffella antiqua (Dutkevich), Pseudostaffella paracompressa Safonova, Pseudostaffella kimi Cheong, Pseudostaffella sp., Beedeina lanceolata (Lee and Chen), Beedeina samarica (Rauser-Chernoussova), Beedeina sp. A, Neostaffella sphaeroidea cuboides Rauser-Chernoussova, and Hanostaffella hanensis Cheong. Such fusulinids species were reported from the lower part of the Geumcheon Formation in Samcheok coalfield and the middle Moscovian stage in Eurasia. On the basis of the fusulinid biostratigraphic correlation of the Gangdong geologic sections (A) to (C), the limestone should be overlapped by faults and folds. Moreover the stratigraphic thickness of the limestone is thinner than the thickness of the limestone outcrop of the Gangdong geologic section. Therefore, the stratigraphic sequence of the Gangdong geologic section is represented as the Gangdong geologic section (A).

Correlation of CT Perfusion Images with VEGF Expression in Solitary Brain Metastases

  • Zhang, Jian-Hua;Wang, Ming-Sheng;Pan, Hai-Hong;Li, Shu-Feng;Wang, Zhong-Qiu;Chen, Wang-Sheng
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1575-1578
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    • 2012
  • Objectives: To obtain permeability surface (PS) values using multi-slice helical CT perfusion imaging and to evaluate the spatial distribution correlation between PS values and vascular endothelial growth factor (VEGF) expression in solitary brain metastases. Methods: Imaging was performed on 21 patients, PS values being calculated from the central, border and peripheral parts of tumours. VEGF expression was determined by immunohistochemical staining. Results: Rim enhancement was found in 16 cases, the border of the tumour featuring PS elevation with high VEGF expression in 13 cases. In the 5 cases with nodular enhancement, the border and the central part had high permeability and VEGF expression was high in all cases, the correlation being significant (P<0.01). Conclusion: VEGF expression in brain metastases positively correlates with PS values from CT perfusion imaging, so that the latter can be used in the surveillance of angiogenic activity in brain metastases.

A comparative study on Chinese batik of Miao and Buyi in GuiZhouSheng (중국 귀주성의 묘족과 포의족의 납염 비교 연구)

  • Meng, Yu;Choi, Jongmyoung;Kim, Jiyoung
    • The Research Journal of the Costume Culture
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    • v.26 no.4
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    • pp.531-546
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    • 2018
  • The purpose of this study is to comparatively examine techniques and expressive characteristics of batik by focusing on the work of DanZhai and that of ZhenNing Buyi. We collected 50 pieces of batik each from Miao and Buyi in Chinese GuiZhouSheng and then analyzed their colors and patterns. The findings show that most of Miao's batik had deep and soft tones, while that of Buyi most often had blackish tone since the number of dyes in the latter is higher than that in the former. Miao often used patterns expressing animals or animals and plants, while Buyi frequently used geometric ones. An examination of specific motifs demonstrated that butterflies, fish, and flowers were most often used by Miao, while dots, vortexes, lines, and lozenges were frequently utilized by Buyi. For expressive techniques, both Miao and Buyi commonly used simple stylized expression or complex expression combining stylized and geometric techniques. Maio's motifs were often decoratively expressed with delicate curves, while Buyi's motifs had simple structures, so pictures were neatly expressed with thick lines and wide and simple planes. For the composition of the motifs, incomplete symmetry between top and bottom and between left and right often appeared in Maio, while in Buyi complete symmetry was frequently shown.