• 한국패류학회지
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• 제31권4호
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• pp.257-262
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• 2015

The seven selected primers BION-13, BION-29, BION-61, BION-64, BION-68, BION-72 and BION-80 generated the total number of loci, average number of loci per lane and specific loci in Meretrix lusoria (ML), Saxidomus purpuratus (SP) and Cyclina sinensis (CS) species. Here, the complexity of the banding patterns varied dramatically between the primers from the three venerid clam species. The higher fragment sizes (> 1,000 bp) are much more observed in the SP species. The primer BION-68 generated 21 unique loci to each species, which were ascertaining each species, approximately 150 bp, 300 bp and 450 bp, in the ML species. Remarkably, the primer BION-80 detected 7 shared loci by the three clam species, major and/or minor fragments of sizes 500 bp, which were matching in all samples. As regards average bandsharing value (BS) results, individuals from CS clam species (0.754) exhibited higher bandsharing values than did individuals from SP clam species (0.607) (P < 0.05). In this study, the dendrogram obtained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (LUSORIA01-LUSORIA07), cluster 2 (PURPURATUS08-PURPURATUS14), cluster 3 (SINENSIS15-SINENSIS21). Among the twenty one venerid clams, the shortest genetic distance that displayed significant molecular differences was between individuals 18 and 20 from the CS species (genetic distance = 0.071), while the longest genetic distance among the twenty-one individuals that displayed significant molecular differences was between individuals LUSORIA no. 02 and PURPURATUS no. 09 (genetic distance = 0.778). Relatively, individuals of SP venerid species were appropriately closely related to that of CS species, as shown in the hierarchical dendrogram of genetic distances. Eventually, PCR fragments exposed in the present study may be worthwhile as a DNA marker the three venerid clam species to discriminate.

• 한국의류산업학회지
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• 제9권1호
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• pp.49-54
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• 2007

In the 21th century, the age of image, people express and evaluate with images. Image is a symbol of a person or a thing. It simulates people's visual sense most quickly and precisely and is shared in the society. At the time, since it is acknowledged that there are few books available and lack of theoretical system with regard to image making, the purpose of this study was to get a theoretical access to image making by taking as an empirical case the case of Choi Byeongryeol, a candidate running for the congress in June 4, 1998 and combining it with theories of costume. Research methods employed here were to investigate the theoretical system of image making and fashion direction, and to analyze empirical cases. The boundary of the empirical case was limited to the candidate's fashion direction during the election period for 50 days from April 1998 to the election date June 4, 1998. The results of this study are as follows. First, personal image making aims at the establishment of one's own identity through building up an ideal image. Second, it is found that personal image making can make a complete image possible through fashion direction. Third, it is found that fashion direction functions as a symbol and communicative means with a result that the effect can penetrate to the society accurately and quickly. Fourth, it is found that fashion direction fit for a situation can enhance personal values and reinforce his or her competitive power to carry out the ultimate goals in the society. This study proved that fashion styling for personal image making expresses a person in a symbolic image, enhances his/her personal value in the society and, ultimately, contributes to the establishment of an individual's identity.

• 한국작물학회지
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• 제48권4호
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• pp.326-333
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• 2003

Transgenic rice plants expressing a Bacillus subtilis protoporphyrinogen oxidase (Protox), the last shared enzyme of the porphyrin pathway in the expressed cytoplasm or the plastids, were compared with non-trangenic rice plants in their growth characteristics such as tiller number, plant height, biomass, and yield. Transgenic rice plants of $\textrm{T}_3$ generation had 8 to 15 % and 25 to 43% increases in tiller number compared to non-transgenic rice plants at 4 and 8 weeks after transplanting(WAT); similar values were observed for $\textrm{T}_4$ generation at 4 and 8 WAT. However, the plant height in both $\textrm{T}_3$ and $\textrm{T}_4$ generations was similar between transgenic rice plants and non-transgenic rice plants at 4 and 8 WAT. Transgenic rice plants had 13 to 32% increase in above-ground biomass and 9 to 28% increase in grain yield compared to non-transgenic rice plants, demonstrating that biomass and yield correlate with each other. The increased grain yield of the transgenic rice plants was closely associated with the increased panicle number per plant. The percent of filled grain, thousand grains and spikelet number per panicle were similar between transgenic and non-transgenic rice plants. Generally, the growth and yield of transgenic generations ($\textrm{T}_2$, $\textrm{T}_3$, and $\textrm{T}_4$) and gene expressing sites (cytoplasm-expressed and plastid-targeted transgenic rice plants) were similar, although they slightly varied with generations as well as with gene expressing sites. The transgenic rice plants had promotive effects, indicating that regulation of the porphyrin pathway by expression of B. subtilis Protox in rice influences plant growth and yield.

• 한국도서관정보학회지
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• 제49권3호
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• pp.283-305
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• 2018

대학도서관의 예산은 감소하는데 반해 해외 출판사의 인쇄학술지 및 전자저널 구독비용은 꾸준하게 상승하고 있다. 이에 따라 교육부와 KERIS는 해외 학술자료에 대한 접근성 확보차원에서 해외 인쇄학술지 분담수서 및 공동 활용을 위한 외국학술지지원센터 사업을 2006년부터 시행하고 있다. 본 연구에서는 최근 수년 동안의 FRIC 서비스 및 이용현황을 살펴보고, FRIC 담당자 10명과의 심층면담을 통해 FRIC의 역할 및 가치, 운영상의 이해관계자별 주요 이슈, 서비스 개선방안 및 신규 서비스 요소를 조사하였다. 연구결과, FRIC은 지속적으로 발전하여 활발하게 운영되고 있어 학술정보의 공동이용과 학술연구 진흥에 기여하고 있는 것으로 나타났다. 그렇지만 FRIC 운영에 있어 5가지 유형의 이해관계자 즉, 교육부 및 KERIS, 대학본부 및 도서관, 이용자, 타 기관 FRIC, 출판사 및 구독 대행사 등은 각기 다른 다양한 이슈사항을 가지고 있었다. 따라서 본 연구에서는 이러한 이슈사항을 해결하기 위한 개선방안을 제도, 시스템, 운영 및 서비스 측면에서 제안하였다.

• BMB Reports
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• 제39권1호
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• pp.46-54
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• 2006

The coenzyme A-acylating 2-oxoacid:ferredoxin oxidoreductase and ferredoxin (an effective electron acceptor) were purified from the hyperthermophilic archaeon, Sulfolobus solfataricus P1 (DSM1616). The purified ferredoxin is a monomeric protein with an apparent molecular mass of approximately 11 kDa by SDS-PAGE and of $11,180{\pm}50$ Da by MALDI-TOF mass spectrometry. Ferredoxin was identified to be a dicluster, [3Fe-4S][4Fe-4S], type ferredoxin by spectrophotometric and EPR studies, and appeared to be zinc-containing based on the shared homology of its N-terminal sequence with those of known zinc-containing ferredoxins. On the other hand, the purified 2-oxoacid: ferredoxin oxidoreductase was found to be a heterodimeric enzyme consisting of 69 kDa $\alpha$ and 34 kDa $\beta$ subunits by SDS-PAGE and MALDI-TOF mass spectrometry. The purified enzyme showed a specific activity of 52.6 units/mg for the reduction of cytochrome c with 2-oxoglutarate as substrate at $55^{\circ}C$, pH 7.0. Maximum activity was observed at $70^{\circ}C$ and the optimum pH for enzymatic activity was 7.0 -8.0. The enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutyrate, and 2-oxoglutarate. Among the 2-oxoacids tested (pyruvate, 2-oxobutyrate, and 2-oxoglutarate), 2-oxoglutarate was found to be the best substrate with $K_m$ and $k_{cat}$ values of $163\;{\mu}M$ and $452\;min^{-1}$, respectively. These results provide useful information for structural studies on these two proteins and for studies on the mechanism of electron transfer between the two.

• 한국실내디자인학회논문집
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• 제21권2호
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• pp.23-34
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• 2012

After the modern age, the rapid urbanizationhad a big impact on the then architecture. R. Venturi and A. Rossi are two of the leading architects, developing architecture in cities in the US and Europe respectively. This study shed light on a tangible and intangible meaning and interpretation of urban landscapes through their architectural thoughts and architectures. The followings are the physical and intangible meaning and interpretation in architectural thoughts and works of those two architects. Venturi understood that iconological landscapes at the roadside in large citiesare the nature of physical landscapes. To Venturi, the façades of buildings at the roadside are a part of signage such as traffic lights and road signs, and those façades have the meaning of symbolic systems beyond simple physical landscapes. To A. Rossi, types of buildings as physical townscapes are a key role supporting raw data of classification in architecture. And also, those types have significance of the basic data shedding light on the principles and history of cities. For intangible factors in R. Venturi's architecture, daily routine, function and use, time, a use for a building and others form complex architecture. And also, those factors describe shared values of the same period as the façades of buildings and complex symbols and formative lexicons in metaphorical terms. For A. Rossi's intangible factors, 'collective memory' is buried in inhabitants of the city, and with that, the city is a place for memory to its inhabitants. What is more, cities' monuments have intangible landscapes like 'sustainability', 'permanence' and so on. With lots of events happening throughoutcities, those monuments are the whole images of cities giving the value to the urban buildings that reside in cities. Finally, R. Venturi's all-encompassing complex architecture concept was extended on a tangible and intangible point of townscapes. It was found that A. Rossi's tangible thought was formed from the whole landscape of historic cities in then Italy as the background of time and place. Also, With types of urban buildings and 'collective memory', A. Rossi drew architectural norms and formats of unchangeable types.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1263-1269
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• 2014

Comprehensive information on genetic diversity and introgression is desirable for the design of rational breed improvement and conservation programs. Despite the concerns regarding the genetic introgression of Western pig breeds into the gene pool of the Korean native pig (KNP), the level of this admixture has not yet been quantified. In the present study, we genotyped 93 animals, representing four Western pig breeds and KNP, using the porcine SNP 60K BeadChip to assess their genetic diversity and to estimate the level of admixture among the breeds. Expected heterozygosity was the lowest in Berkshire (0.31) and highest in Landrace (0.42). Population differentiation ($F_{ST}$) estimates were significantly different (p<0.000), accounting for 27% of the variability among the breeds. The evidence of inbreeding observed in KNP (0.029) and Yorkshire (0.031) may result in deficient heterozygosity. Principal components one (PC1) and two (PC2) explained approximately 35.06% and 25.20% of the variation, respectively, and placed KNP somewhat proximal to the Western pig breeds (Berkshire and Landrace). When K = 2, KNP shared a substantial proportion of ancestry with Western breeds. Similarly, when K = 3, over 86% of the KNP individuals were in the same cluster with Berkshire and Landrace. The linkage disquilbrium (LD) values at $r^2_{0.3}$, the physical distance at which LD decays below a threshold of 0.3, ranged from 72.40 kb in Landrace to 85.86 kb in Yorkshire. Based on our structure analysis, a substantial level of admixture between Western and Korean native pig breeds was observed.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1753-1759
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• 2006

Paenibacillus sp. HY-8 isolated from the digestive tracts of the longicorn beetle, Moechotypa diphysis, produced an extracellular endoxylanase with a molecular weight of 20 kDa estimated by SDS-PAGE. The xylanase was purified to near electrophoretic homogeneity from the culture supernatant after ammonium sulfate precipitation, gel filtration, and ionexchange chromatography. The purified xylanase exhibited the highest activities at pH 6.0 and $50^{\circ}C$. The $K_m\;and\;V_{max}$ values were 7.2 mg/ml and 16.3 U/mg, respectively, for birchwood xylan as the substrate. Nucleotide sequence of the PCR-cloned gene was determined to have the open reading frame encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequence analyses predicted that the precursor xylanase contained a signal peptide composed of 28 amino acids and a catalytically active 19.9-kDa peptide fragment. The deduced amino acid sequence shared extensive similarity with those of the glycoside hydrolase family 11 of xylanases from other bacteria. The predicted amino acid sequence contained two glutamate residues, previously identified as essential and conserved for active sites in other xylanases of the glycoside hydrolase family 11.

• 한국콘텐츠학회논문지
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• 제9권5호
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• pp.76-83
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• 2009

최근 공동 연결 관계와 연속적인 정점 위치들의 움직임으로 이루어진 메쉬들의 모임, 즉 메쉬 시퀀스를 압축하는 연구가 활발하게 이루어지고 있다. 본 논문은 Khodakovsky 등이 제시한 준균일 메쉬 압축방법에 기반한 메쉬 시퀀스의 압축 알고리즘을 제시하고자 한다. 준균일 메쉬 시퀀스로의 메쉬 재구성을 이용한 메쉬 시퀀스 압축 알고리즘은 크게 두 부분으로 이루어진다. 첫 번째 부분은 주어진 비균일 메쉬 시퀀스로부터 준균일 메쉬 시퀀스를 생성하는 것이다. 준균일 메쉬를 생성하기 위해 본 논문에서는 MAPS 알고리즘을 사용하였다. 하지만 단일 메쉬에 대해 적용이 가능한 MAPS 알고리즘을 메쉬 시퀀스에 그대로 적용할 수 없다. 따라서 주어진 애니메이션에서의 정점 움직임을 고려하여 유사한 움직임을 가지는 영역별로 분할하고, 이 분할 정보과 정점의 움직임을 고려할 수 있도록 MAPS 알고리즘을 확장하였다. 두 번째 단계에서는 웨이블릿 변형과 메쉬 분할 정보를 이용해 준균일 메쉬를 압축하였다. 각 분할 영역의 변환 정보를 고려해 분할 영역 내 정점의 위치를 예측하고, 참조 프레임과의 차이값을 압축함으로써 효율적으로 준균일 메쉬 시퀀스를 압축하였다.

• Spatial Information Research
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• 제11권4호
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• pp.493-508
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• 2003

GIS 기반의 토지데이터베이스가 수직적 수평적 행정체계 속에서 공유되고 언제 어디서나 국민에게 서비스되기 위해서는 다음의 문제가 해결되어야 한다. 첫째 이질 환경, 분산 환경에서 운영되고 있는 시·군·구의 개별 토지종합정보망을 수직적 수평적으로 연결한 GIS 서비스 네트워크로 묶어야 한다. 둘째는 이와 같이 묶은 GIS 서비스 네트워크에 쉽게 접근하여 원하는 자료를 검색할 수 있는 메카니즘이 있어야 한다. 이를 위해 정보기술발전 동향과 전자정부 구현요구를 감안하여 다양한 정보시스템을 단계적으로 Web기반의 통합 시스템으로 발전시키는 전략이 필요하다. 본 연구에서는 시·군·구 중심으로 구축된 토지종합정보망의 GIS자료를 언제 어디서나 제공할 수 있도록 시·군·구-광역시 도-중앙부처의 개별 서비스 시스템을 통합하여 GIS 서비스 네트워크를 구축하는 프레임워크를 제시했다.