The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Genetic distances of three venerid species identified by PCR analysis

  • Jeon, Jun-Hyub (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Yoon, Jong-Man (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • Received : 2015.11.23
  • Accepted : 2015.12.30
  • Published : 2015.12.30
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Abstract

The seven selected primers BION-13, BION-29, BION-61, BION-64, BION-68, BION-72 and BION-80 generated the total number of loci, average number of loci per lane and specific loci in Meretrix lusoria (ML), Saxidomus purpuratus (SP) and Cyclina sinensis (CS) species. Here, the complexity of the banding patterns varied dramatically between the primers from the three venerid clam species. The higher fragment sizes (> 1,000 bp) are much more observed in the SP species. The primer BION-68 generated 21 unique loci to each species, which were ascertaining each species, approximately 150 bp, 300 bp and 450 bp, in the ML species. Remarkably, the primer BION-80 detected 7 shared loci by the three clam species, major and/or minor fragments of sizes 500 bp, which were matching in all samples. As regards average bandsharing value (BS) results, individuals from CS clam species (0.754) exhibited higher bandsharing values than did individuals from SP clam species (0.607) (P < 0.05). In this study, the dendrogram obtained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (LUSORIA01-LUSORIA07), cluster 2 (PURPURATUS08-PURPURATUS14), cluster 3 (SINENSIS15-SINENSIS21). Among the twenty one venerid clams, the shortest genetic distance that displayed significant molecular differences was between individuals 18 and 20 from the CS species (genetic distance = 0.071), while the longest genetic distance among the twenty-one individuals that displayed significant molecular differences was between individuals LUSORIA no. 02 and PURPURATUS no. 09 (genetic distance = 0.778). Relatively, individuals of SP venerid species were appropriately closely related to that of CS species, as shown in the hierarchical dendrogram of genetic distances. Eventually, PCR fragments exposed in the present study may be worthwhile as a DNA marker the three venerid clam species to discriminate.

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References

  1. Chenyambuga, S.W., Hanotte, O., Hirbo, J., Watts, P.C., Kemp, S.J., Kifaro, G.C., Gwakisa, P.S., Petersen, P.H. and Rege, J.E.O. (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australasian Journal of Animal Sciences, 17: 445-452. https://doi.org/10.5713/ajas.2004.445
  2. Huang, B.X., Peakall, R. and Hanna, P.J. (2000) Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Marine Biology, 136: 207-216. https://doi.org/10.1007/s002270050678
  3. Jeffreys, A.J. and Morton D.B. (1987) DNA fingerprints of dogs and cats. Animal Genetics, 18: 1-15.
  4. Jin, Y. G., Oh, B. S., Jung, C.K., Kim, T.I. and Park, M.W. (2011) Survival and growth of the purplish Washington clam, Saxidomus purpuratus spat sowed in bottom and intermediate culture. Korean Journal of Malacology, 27: 199-204 (in Korean). https://doi.org/10.9710/kjm.2011.27.3.199
  5. Ju, S. M. and Lee, J. S. (2011) Ultrastructure of the Digestive Diverticulum of Saxidomus purpuratus (Bivalvia: Veneridae). Korean Journal of Malacology, 27: 159-165 (in Korean). https://doi.org/10.9710/kjm.2011.27.3.159
  6. Jung, H.T., Kim, J., Shin, J.A., Sohn, H.Y., Choi, S.D. (2004) Genetic relationship of the five venerid clams (Bivalvia, Veneridae) in Korea. Journal of Aquaculture, 17: 251-257 (in Korean).
  7. Kim, Y.H., Chang, D.S., Cha, B.R. and Park, Y.C. (2001) Estimation of population size of Fulvia mutica (Reeve) in Gwangyang Bay. Abstracts 2001 Joint Meeting of the Korean Society on Fisheries Science: 400-401 (in Korean).
  8. Kim, S.J. Chung, Y.J. and Lee, T.K. (2011) In vivo comet assay on flounder and clam exposed to BaP and TBT. Ocean and Polar Research, 33: 127-133 (in Korean). https://doi.org/10.4217/OPR.2011.33.2.127
  9. Kim, Y.H., Kwon, D.H., Chang, D.S., Kim, J.B., Kim, S.T.and Ryu, D.K. (2007) Stock assessment of purplish Washington clam, Saxidomus purpuratus in the southern coastal waters of Korea. Korean Journal of Malacology, 23: 31-38 (in Korean).
  10. Kim, J.Y, Park, C.Y. and Yoon, J.M. (2004) Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean Journal of Genetics, 26: 123-134.
  11. Mahmoud, N., Dellali, M., El Bour, M., Aissa, P. and Mahmoudi, E. (2010) The use of Fulvia fragilis (Mollusca: Cardiidae) in the biomonitoring of Bizerta lagoon: A multimarkers approach. Ecological Indicators, 10: 696-701. https://doi.org/10.1016/j.ecolind.2009.11.010
  12. McCormack, G. C., Powell, R and Keegan, B. (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Marine Biotechnology, 2: 100-106.
  13. Min, D.K. (2001) Korea mollusks with color illustration. Min shell house, pp. 267-275.
  14. Park, S.Y., Park, J.S. and Yoon, J.M. (2005) Genetic difference and variation in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli) throughout its distribution range determined by RAPD-PCR Analysis. Korean Journal of Genetics, 27: 307-317, 2005.
  15. Shin, Y.K., Choi, N.J., Oh, B.S., Jung, A.J. and Kim, S.Y. (2007) Gonad development and reproductive cycle of the purplish Washington clam, Saxidomus purpuratus Bivalvia: Veneridae) from Gangjin Bay. Korean Journal of Malacology, 23: 165-172 (in Korean).
  16. Tassanakajon, A., Pongsomboon, S., Jarayabhand, P., Klinbunga, S. and Boonsaeng, V. (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA?analysis. Journal of Marine Biotechnology, 6: 249-254.
  17. Yoon, J.M. and Kim, Y.H. (2003) Wide marsh clam (Corbicula spp.) populations from three sites analysed by RAPD-PCR-AGE. Bulletin of Electrochemistry, 19: 337-348.
  18. Yoon, J.M. and Kim, J.Y. (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analyzed by RAPD-PCR. Asian-Australasian Journal of Animal Sciences, 17: 1053-1061. https://doi.org/10.5713/ajas.2004.1053
  19. Yoke-Kqueen, C. and Radu, S. (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. Journal of Biotechnology, 127: 161-166. https://doi.org/10.1016/j.jbiotec.2006.06.001