• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.224-230
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• 1999

The ddh gene encoding meso-DAP-dehydrogenase (DDH) involved in the dehydrogenase pathway is essential for high-level lysine production in Brevibacterium lactofermentum. To investigate its influence on lysine production by overexpression of the ddh gene in a lysine-producing B. lactofermentum, recombinant plasmid pRK1 and pRK31 containing the ddh gene of B. lactofermentum were constructed and they were introduced into B. lactofermentum by electroporation. Multiple copies of pRK1 and pRK31 caused 7-fold and 14-fold increase of DDH activity in B. lactofermentum cell extracts, respectively. As determined in shake flask fermentation, lysine production of B. lactofermentum harboring pRK1 or pRK31 was 22% or 19% higher than that of the control, respectively.

• Earthquakes and Structures
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• v.19 no.3
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• pp.157-174
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• 2020

The in-situ pushover test differs from the shake-table test because it is performed outdoors and thus its size is not restricted by space, which allows us to test a full-size building. However, to build a new full-size building for the test is not economical, consequently scholars around the world usually make scale structures or full-scale component units to be tested in the laboratory. However, if in-situ pushover tests can be performed on full-size structures, then the seismic behaviors of buildings during earthquakes can be grasped. In view of this, this study conducts two in-situ pushover tests of reinforced concrete (RC) buildings. One is a masonry-infilled RC building with openings (the openings ratio of masonry infill wall is between 24% and 51%) and the other is an RC building without masonry infill. These two in-situ pushover tests adopt obsolescent RC buildings, which will be demolished, to conduct experiment and successfully obtain seismic capacity curves of the buildings. The test results are available for the development or verification of a seismic evaluation model. This paper uses ASCE 41-17 as the main evaluation model and is accompanied by a simplified pushover analysis, which can predict the seismic capacity curves of low-rise buildings in Taiwan. The predicted maximum base shear values for masonry-infilled RC buildings with openings and for RC buildings without masonry infill are, respectively, 69.69% and 87.33% of the test values. The predicted initial stiffness values are 41.04% and 100.49% of the test values, respectively. It can be seen that the ASCE 41-17 evaluation model is reasonable for the RC building without masonry infill walls. In contrast, the analysis result for the masonry infilled RC building with openings is more conservative than the test value because the ASCE 41-17 evaluation model is limited to masonry infill walls with an openings ratio not exceeding 40%. This study suggests using ASCE 41-17's unreinforced masonry wall evaluation model to simulate a masonry infill wall with an openings ratio greater than 40%. After correction, the predicted maximum base shear values of the masonry infilled RC building with openings is 82.60% of the test values and the predicted initial stiffness value is 67.13% of the test value. Therefore, the proposed method in this study can predict the seismic behavior of a masonry infilled RC frame with large openings.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.689-698
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• 2013

The production and characterization of an active recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in Escherichia coli BL21(DE3) has been previously reported. In this study, the effect of the signal peptide (SP), inducer concentration, process scale, and operational mode (batch and semi-continuous) on GALNS production were evaluated. When native SP was presented, higher enzyme activity levels were observed in both soluble and inclusion bodies fractions, and its removal had a significant impact on enzyme activation. At shake scale, the optimal IPTG concentrations were 0.5 and 1.5 mM for the strains with and without SP, respectively, whereas at bench scale, the highest enzyme activities were observed with 1.5 mM IPTG for both strains. Noteworthy, enzyme activity in the culture media was only detected when SP was presented and the culture was carried out under semi-continuous mode. We showed for the first time that the mechanism that in prokaryotes recognizes the SP to mediate sulfatase activation can also recognize a eukaryotic SP, favoring the activation of the enzyme, and could also favor the secretion of the recombinant protein. These results offer significant information for scaling-up the production of human sulfatases in E. coli.

• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1308-1321
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• 2013

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average $1.85{\pm}0.11{\times}10^6$ cells/ml against $0.60{\pm}0.08{\times}10^6$ cells/ml for CultiSpher-S), mAb production ($2.04{\pm}0.41{\mu}g/ml$ against $0.99{\pm}0.35{\mu}g/ml$ for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, $4{\times}10^5$ cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.

• The Korean Journal of Mycology
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• v.33 no.2
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• pp.59-63
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• 2005

Altogether twenty isolates were collected from Wakayama, Japan. The optimum mycelial growth of I. obliquus was observed in BMYA and MCM. The optimum temperature and pH for the mycelial growth were $25^{\circ}C\;and\;6.0{\sim}7.0$, respectively. The optimum carbon and nitrogen sources were dextrose and yeast extract, respectively. Similarly, the optimum mineral salt was $K_{2}HPO_{4}$. The optimum number of mycelial discs for the mycelial growth was $6{\sim}7$ per 100 ml. Similarly, the optimum culture period was $21{\sim}22$ days in liquid broth. The optimum brown rice : water ratio was 1 : 1. No difference in mycelial growth was observed in all the four types of tree stumps used.

• Korean journal of communication and information
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• v.81
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• pp.189-224
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• 2017

The case of 'Paik Jong-moon's Taped Conversation,' has presented "an important and serious challenge to the freedom of the press and democracy" in South Korea. Nevertheless, this case has not been reported by the mainstream news media. It has also been forgotten without a proper fact-finding or investigation by regulatory agencies, like The Foundation for Broadcast Culture, The Korea Communications Commission, or The National Assembly. This study aims to examine why the above has happened through in-depth interviews of reporters and TV producers, senior journalists, former and incumbent commissioners of the broadcasting regulatory agencies, and experts of the industry, as well as literature research. Here, I present three answers. First, I found two reasons the mainstream press has ignored this incident. 1) It serves for political interests instead of reporting truth. 2) Public broadcasters' watchdog role has been neutralized. Second, regulatory agencies like The Foundation for Broadcast Culture, The Korea Communications Commission and The National Assembly are ruled by political tribalism. The ruling party's members of the National Assembly and these agencies were reluctant to investigate allegations surrounding Paik Jong-moon, such as illegal dismissals, illegal intervention in programming or production, illegal recruitment and illegal business deals. That's because they considered CEO Paik an ally. Using their majority power, they have rejected the request from opposition-affiliated commissioners or from opposition lawmakers to investigate the allegations. Third, there were no alternative forces within the public broadcasters to unveil the truth. In conclusion, the legal and institutional shake-up of corporate governance is urgently needed for public broadcasters and broadcasting regulatory agencies.

• 한국HCI학회:학술대회논문집
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• 2008.02b
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• pp.651-655
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• 2008

It provides a three-dimensional virtual experience consisting of information presented to and perceived by the senses of the user. Recently, the advent of a virtual avatar, which mimics the appearance and behavior of humans, enable that the virtual reality (VR) can provide not only a virtual space but also a virtual society to be interact with a virtual avatar which represents humans. But, it is impossible that the user directly interacts with the VE in previous studies, though direct interactions occur in real social relationship. Therefore, In this study, we know that the cognition about a avatar when the user directly interacts with the virtual avatar in the VE. In order to investigate this purpose, we performed a fMRI study using VE that a avatar accept or reject the user's offer when the user offer his (or her) hand to a avatar. In result of questionnaire about the user's feeling by avatar's action, the user feels about avatar's acceptance action that the avatar acts positively and suitably. In contrast the user feels about avatar's rejective action that the avatar acts negatively and disapprovingly. In result of fMRI analysis, the primary visual area, the visual association area, the SMA, the premotor area, the cerebellum and etc, activate in common with the other avatar's acceptance action and rejective action. The results show that the subject recognizes not only avatars as social objects but also avatar's action as socially meaningful action. In other words, it is possible to transfer social context and emotion through avatar's action.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.217-222
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• 2010

Bark is a general medium for potted Cymbidium in Korea, but it is difficult to shake off or eliminate the medium from the roots before exporting the potted plants. This working process can injure the roots and then deteriorate plant marketability. This study was carried out to select the optimum medium easier to eliminate from the roots instead of bark and the optimum amount of applied fertilizer to improve the plant growth. Cymbidium young plants 'Honey Hot' and 'Desert Look' were planted in pots with bark, cocochip, and peatmoss. The plants were treated with 2, 4, and 6 g of slow release fertilizer. The plant growth characteristics were investigated in the first and second years during production period of three years. The medium characteristics and mineral nutrient content of the leaves were also examined in the second year. In the first year, the plant growth of 'Desert Look' was improved in all peatmoss treatments more than bark. 'Honey Hot' showed the highest plant growth values in the bark treatment. In the second year, the plant growth of the two cultivars was improved in peatmoss. Cocochip treatments showed the lower plant growth values than bark and peatmoss in the first and second year. There was no significant difference among fertilizer amounts in all the media. The higher CEC values of peatmoss medium resulted to higher capacity to hold more nutrients than bark, and the nutrient retention of the peatmoss improved the plant growth. The higher K and Ca contents in the leaves would contribute to improve the plant growth. Consequently, it would be possible to use peatmoss instead of bark for Cymbidium young plants, but there must be always attention to appropriately water the medium and manage the moisture.

• Interaction and multiscale mechanics
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• v.2 no.1
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• pp.69-89
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• 2009

Reinforced and prestressed concrete (RC and PC) thin walls are crucial to the safety and serviceability of structures subjected to shear. The shear strengths of elements in walls depend strongly on the softening of concrete struts in the principal compression direction due to the principal tension in the perpendicular direction. The past three decades have seen a rapid development of knowledge in shear of reinforced concrete structures. Various rational models have been proposed that are based on the smeared-crack concept and can satisfy Navier's three principles of mechanics of materials (i.e., stress equilibrium, strain compatibility and constitutive laws). The Cyclic Softened Membrane Model (CSMM) is one such rational model developed at the University of Houston, which is being efficiently used to predict the behavior of RC/PC structures critical in shear. CSMM for RC has already been implemented into finite element framework of OpenSees (Fenves 2005) to come up with a finite element program called Simulation of Reinforced Concrete Structures (SRCS) (Zhong 2005, Mo et al. 2008). CSMM for PC is being currently implemented into SRCS to make the program applicable to reinforced as well as prestressed concrete. The generalized program is called Simulation of Concrete Structures (SCS). In this paper, the CSMM for RC/PC in material scale is first introduced. Basically, the constitutive relationships of the materials, including uniaxial constitutive relationship of concrete, uniaxial constitutive relationships of reinforcements embedded in concrete and constitutive relationship of concrete in shear, are determined by testing RC/PC full-scale panels in a Universal Panel Tester available at the University of Houston. The formulation in element scale is then derived, including equilibrium and compatibility equations, relationship between biaxial strains and uniaxial strains, material stiffness matrix and RC plane stress element. Finally the formulated results with RC/PC plane stress elements are implemented in structure scale into a finite element program based on the framework of OpenSees to predict the structural behavior of RC/PC thin-walled structures subjected to earthquake-type loading. The accuracy of the multiscale modeling technique is validated by comparing the simulated responses of RC shear walls subjected to reversed cyclic loading and shake table excitations with test data. The response of a post tensioned precast column under reversed cyclic loads has also been simulated to check the accuracy of SCS which is currently under development. This multiscale modeling technique greatly improves the simulation capability of RC thin-walled structures available to researchers and engineers.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.9
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• pp.127-135
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• 2013

In order to acquire images in low-light environments, it is usually necessary to adopt long exposure times or resort to flash lights. However, flashes often induce color distortion, cause the red-eye effect and can be disturbing to subjects. On the other hand, long-exposure shots are susceptible to subject-motion, as well as motion-blur due to camera shake when performed hand-held. A recently introduced technique to overcome the limitations of traditional low-light photography is that of multi-spectral flash. Multi-spectral flash images are a combination of UV/IR and visible spectrum information. The general idea is that of retrieving details from the UV/IR spectrum and color from the visible spectrum. However, multi-spectral flash images themselves are subject to color distortion and noise. This works presents a method to compute multi-spectral flash images so that noise can be reduced and color accuracy improved. The proposed approach is a previously seen optimization method, improved by the introduction of a weight map used to discriminate uniform regions from detail regions. The weight map is generated by applying canny edge operator and it is applied to the optimization process for discriminating the weights in uniform region and edge. Accordingly, the weight of color information is increased in the uniform region and the detail region of weight is decreased in detail region. Therefore, the proposed method can be enhancing color reproduction and removing artifacts. The performance of the proposed method has been objectively evaluated using long-exposure shots as reference.