• 대한예방한의학회지
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• 제12권1호
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• pp.127-134
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• 2008

This study was carried out in 14,443 subjects(8,706 men, 5,737 women, mean age $41.8{\pm}9.16$) who visited the health promotion center of one university hospital. Serum $T_3$, $T_4$ and TSH concentrations were measured with CIA using commercial kits on ADVIA Centaur Assay and Serum total cholesterol, triglyceride, and HDL-cholesterol were measured by auto-analyzer Hitach 7600 in enzyme method. 1. The prevalence of subclinical hypothyroidism was 12.6/1,000 population(men 6.0/1,000, women 22.7/1,000) and as high as about 4 times in women than in men. 2. In subclinical hypothyroidism, the values of total cholesterol and LDL-cholesterol were significantly high by 5.5 mg/dL, 4.6 mg/dL compared with normal group(p<0.05). 3. In all the tested hypercholesterolemia and hyperLDL-cholesterolemia, were correlated with TSH. There is higher correlation in women group than men group. These results indicate that the subclinical hypothyroidism and dyslipemia significantly correlated. Especially, it is very important to find that the hypercholesterolemia and hyperLDL-cholesterolemia are the high risk factors of atherosclerosis. Therefore, it is essential for the group of subclinical hypothyroidism to take the cholesterol, LDL-cholesterol and serum lipid test as well as thyroid function test.

• 여성건강간호학회지
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• 제10권4호
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• pp.311-317
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• 2004

Purpose: The purpose of this study was to evaluate the effects of Internet education on plasma glucose and serum lipids in female type 2 diabetic patients. Method: Control and experimental groups were assessed by a pre-test and at 3 months, 6 months, 9 months, and 12 months. Fourteen patients were assigned randomly to an intervention group and 15 to a control group. Patients in the intervention group were requested to input their blood glucose levels everyday by cellular phone or wire Internet for 1 year. The goal of the intervention was to keep blood glucose concentrations close to the normal range (glycosylated hemoglobin, HbA1c<7%). An intervention was applied to the intervention group weekly for 1 year. Optimal recommendations were sent weekly by a short message service from a cellular phone and wire Internet. Results: Patients in the intervention group had a significant mean change in HbA1c, from 7.6% at pre-test to 6.9%, at 3 months and equalized at 6.7% at 12 months. There was no significant time and group differences observed in the serum lipids. Conclusion: These findings indicate that Internet education would improve and maintain the normal range of HbA1c in female type 2 diabetic patients.

• 대한미생물학회지
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• 제3권1호
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• pp.43-49
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• 1968

Japanese encephalitis(JE) shows its explosive epidemicity in the temperate zone of Asia but little is known on the overwintering mechanism. One of the hypotheses on the overwintering mechanism is that the virus overwinters in the hibernating animals. There has been no report on the proliferation of JE virus(JEV) or antibody formation in the snakes. The purpose of this experiment is to explore the mutual relationship between JEV and snake and to clarify whether JEV proliferates and induce antibody formation in snakes. Three species of non-poisonous common snakes were employed. Precipitation test was carried out after injecting calf serum and, HI and neutralization tests were done by injecting JEV into the snakes. The gamma globulin fraction of pre- and post-injection serum were compared by paper chromatography. According to the results, precipitating antibody reaction to calf serum could be observed only at $4^{\circ}C$. It was failed to demonstrate HI antibody formation but neutralizing antibody could be detected in one of the 9 snakes. Although antibody could not be detected in test-tube, tile result of paper chromatography shows the remarkable increase of gamma globulin fraction after the injection. Above results are strongly indicating the antibody formation in the snakes.

• 한국식품영양과학회지
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• 제23권1호
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• pp.31-37
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• 1994

This study was carried out to investigate the effect of the feeding mixture of linseed oil, rich in n-3 PUFA and the sunflower seed oil, rich in n-6 PUFA on the lipid metabolism in the dietary hyperlipidemic rats. After male Sprague-Dawley rats were induced hyperlipidemia by feeding the diet containing lard, butter and cholesterol for 3 weeks, then they were fed with the diet containing lard 3 .0% and butter 12.0% for control, the mixture in different proportion of both linseed oil and sunflower seed oil and antihyperlipidemic drugs for 2 weeks. Analysis of the fatty acid composition of the serum lipoprotein fractions showed following results. In the fatty acid composition of serum lipoprotein , the proportion of C18:2 was dominant in all fractions, C20:5 in LDL and HDL fraction and C22:6 in chylomicron fraction. The ratio of n-3P/n-6P tended to increase gradually as it of the test lipid increased in groups 5 to 9 group and was affected by the fatty acid composition of the test lipids.

• 한국동물위생학회지
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• 제42권1호
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• pp.9-15
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• 2019

Transmissible gastroenteritis (TGE) is a disease confined to pigs of all ages, and can be a significant cause of economic loss in breeding herds, primarily because of the very high piglet mortality. The causative agent is a coronavirus, an enveloped positive strand RNA virus and closely related but non-enteropathogenic porcine respiratory coronavirus (PRCV). Although the TGEV has declined with its innocent relative, PRCV, further genome changes could not be excluded. Therefore, the herd-level immunity against this virus is important for the prevention of disease and should be carefully monitored. The aim of this study is to develop monoclonal antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) which can rapidly and accurately determine a large numbers of serum samples for surveillance purpose, and to compare the ELISA with a TGEV-specific serum neutralization test. The MAC-ELISA was sufficiently achieved, and the comparison with the virus-specific serum neutralization assays for 713 sera from pig farms showed a high correlation ($r^2=0.812$, P<0.001). The specificity and sensitivity of MAC-ELISA for the serum neutralization test 91.9% and 91.6%, respectively, which means that the antibody detected by the MAC-ELISA could be said to be protective antibodies. In conclusion, the developed MAC-ELISA would be very helpful in evaluating protective antibodies against TGEV.

• 대한지역사회영양학회지
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• 제4권2호
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• pp.149-156
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• 1999

This study was due to investigate the dietary intakes, serum lipids and other hematological indices in female adolescent smokers. The subjects were 85 smokers, whose average pack-year(smoking years on the basis of one pack of cigarettes per day) was 1.26 and 87 nonsmokers who were female high school students in Seoul. An anthropometric measreument was performed and % body fat was also analyzed by the Bioelectrical Impedance Fatness Analyzer(GIF-891). Dietary intakes and food habits were examined through questionnaires and nutrient intakes were analyzed by the Computer Aided. Nutritional analysis program for professional(CAN-pro). Serum TG, HDL-cholesterol and total-cholesterol levels were measured with test kits. Serum glucose, albumin, GOT and GPT were measured by automated dry chemistry system, SPOTCHEM 4410. Hemoglobin, hematocrit, RBC, WBC and MCV were determined by Semi Automated Microcell Counter(F-520). All data were statistically analyzed by SAS PC package program. There was no significant difference in the anthropometric measurements between smokers and nonsmokers. The caloric intake in adolescent smokers tended to be higher than that of nonsmokers but the difference was not statistically significant. In addition, there was no significant difference between smokers and nonsmokers in biochemical indices. Analysis of serum lipids showed that the serum levels of total-cholesterol and LDL-cholesterol of nonsmokers were unexpectedly significantly higher(p<0.05) than those of smokers. Overall results indicate that smoking itself with short pack-year in healthy female adolescent did not seem to influence apparent health and nutritional status.

• 한국식품영양학회지
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• 제8권4호
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• pp.318-324
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• 1995

The purpose of this study was to Investigate the response of fasting serum glucose and basal insulin to dietary omega-3 fatty fish in normal subjects. Nineteen healthy female volunteer subjects were divided into two groups, depending on fish preference test. Low ap3 fatty acid group for 7 days received a experimental Inlet containing mackeral fish 100g. Calorie intake was 1780 kcal /day. The average 4ally u-3 fatty acid consumption from fish was 3.87g /day (1.03g EPA, 2.849 DHA) . High n-3 fatty acid group was given 7.74g maine u-3 fatty acid (200g mackeral fish) consisting of 2.06g EP45.68g DHA. Calorie intake was 1815 local /day Fasting blond serum glucose, insulin levels were measured at baseline, 7days after experimental diet. In the beginning the levels of fasting serum glucose, basal insulin were not different between both groups. There were no significant changes in fasting serum glucose, insulin levels by experimental diets. These data indicate that marine ar3 fatty acid consumption have no deleterious effect on glycemic control in normal subjects.

• Clinical and Experimental Pediatrics
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• 제50권5호
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• pp.416-421
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• 2007

Many previous studies have proved that human allergic disease resulted from the formation of antibodies belonging to a unique immunoglobulin isotype termed immunoglobulin E (IgE). Most of IgE-producing plasma cells are found in the lymphoid tissue associated with the gastrointestinal and respiratory tracts. IgE may be found free in the mucosal secretions of these tissues, bound to local mast cells, or distributed by the systemic circulation to mast cells and basophils throughout the body. Total serum IgE concentrations tend to be higher in allergic adults and children compared with non-allergic individuals, but the value of total serum IgE as a screening test for allergic disease is limited. Total serum IgE levels are related to the probability of an individual having detectable allergen-specific IgE. Allergen-specific IgE concentrations vary with a person's age, the degree and duration of the recent allergen or cross-reactive allergen exposure. The value of quantitative assays for allergen-specific IgE has been suggested in recent studies. Serum IgE increases in many non-allergic diseases, including infectious and parasitic diseases. The IgE changes appear to be specific to the infectious agents, whereas non-specific in other diseases. The increased serum IgE in some of these conditions probably results from alterations in immune function. This review summarizes the clinical significance of total and allergen-specific IgE examinations in allergic diseases.

• 대한의생명과학회지
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• 제11권4호
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• pp.539-543
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• 2005

Elevated level of serum cholesterol in humans is a risk factor correlated with the development of coronary heart disease. We have screened lactic acid bacteria from pig and human feces for the development of probiotics which have an anti-cholesterol effect. We have used special media to isolate only lactic acid bacteria and they were subjected to the experiments such as oxgal test, carbohydrate fermentation test. Results from the acid tolerance test and growth test in the presence of oxgal demonstrated that some strains would likely survive in thuman stomach, where acidity is high, and in small intestine, where bile fluid is present. In conclusion, we were able to screen lactic acid bacteria which were tolerant against bile acid and supposed to be prominent to lower the cholesterol level in human serum.

• Toxicological Research
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• 제19권1호
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• pp.73-82
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• 2003

To evaluate influence of Saposhnikovia divaricata (Turcz.) Schischk extract on rat, Saposhnikovia divaricata (Turcz.) Schischk extract was diluted with 0.9% saline (100 mg/ml/kg, 10 mg/ml/kg, and 1 mg/ml/kg, respectively), and each of diluted test material extract was daily treated subcutaneously for 4 weeks and single-treated subcutaneously for 2 weeks. There were no significances in body weight analysis, urinary analysis, and ophthalmological test. However, in serum biochemical analysis and hematological analysis, we found some significances in high and middle dose group compared with control group. These significances in serum biochemical analysis and hematological analysis may be not induced by test material, because it was not found to be significant from control group in histopathological examination. Therefore, it was concluded that NOEL (No Observed Effect Level) of test material extract may be higher than all treatment doses used in this study, and Saposhnikovia divaricata (Turcz.) Schischk extract may be a non-toxic material.