• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.972-981
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• 2009

In this paper, a fed-batch cultivation process in recombinant Escherichia coli BL21(DE3) bacteria, for the production of human soluble catechol-O-methyltransferase (hSCOMT), is presented. For the first time, a straightforward model is applied in a recombinant hSCOMT expression system and distinguishes an initial cell growth phase from a protein production phase upon induction. Specifically, the kinetic model predicts biomass, substrate, and product concentrations in the culture over time and was identified from a series of fed-batch experiments designed by testing several feed profiles. The main advantage of this model is that its parameters can be identified more reliably from distinct fed-batch strategies, such as glycerol pulses and exponential followed by constant substrate additions. Interestingly, with the limited amount of data available, the proposed model accomplishes satisfactorily the experimental results obtained for the three state variables, and no exhaustive process knowledge is required. The comparison of the measurement data obtained in a validation experiment with the model predictions showed the great extrapolation capability of the model presented, which could provide new complementary information for the COMT production system.

• Proceedings of the IEEK Conference
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• summer
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• pp.334-338
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• 2004

In this paper, we suggest a new antenna structure for RFID(Radio Frequency IDentification) reader. Conventional RFID reader uses a loop antenna. The central area of a loop antenna shows a low magnetic field strength especially for the case of a large loop antenna diameter. We propose a parallel-fed multiple loop antenna. Simulation results and measured results show that we can adjust field distribution with the number of turns and diameter of an inner loop antenna to obtain a longer reading distance. Simulation results for the specific case of a proposed antenna structure show that at the center point of a proposed parallel-fed multiple loop antenna, the typical card area averaged magnetic field strength is 2.53A/m, which is higher than the case of a conventional type single loop antenna of 0.44A/m and the case of a series-fed multiple loop antenna of 0.96A/m when we drive with same source signal. We realized the antenna for the case of 13.56MHz RFID reader and the performance of reading distance was much more improved than the case of a conventional antenna.

• Journal of Biosystems Engineering
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• v.24 no.2
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• pp.145-152
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• 1999

Methods of discharging each graded agricultural product are divided into two according to the type of feeding. One is based on feeding objects using a series of specially designed holders mounted with an equal interval. The other is randomly feeding objects while being isolated without a specific interval. In this paper, a real time simultaneous discharge algorithm, which is good for objects randomly fed individually and for multi-objects located along a series of discharge buckets, was developed. And the developed algorithm was implemented to the controller and the performance was verified using the system developed for dried mushrooms. The discharge system used for the experiment was composed of a variable speed conveyor, a series of double channel bucket mounted along both sides of the conveyor, and a series of air nozzles and optic sensors. Developed algorithm worked perfectly and could be directly used for automatic discharge system for randomly feeding agricultural products.

• Proceedings of the KIEE Conference
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• 1988.07a
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• pp.144-147
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• 1988

The current-fed DC-DC converter, which is known as the most stable DC-DC converter, has a two-winding reactor in series with the input. In this paper the new multi-output DC-DC converter circuit, in which the 2nd winding of the reactor is creating the 2nd output, while the 2nd winding is feeding the energy to input in the current-fed converter, is proposed. The steady state characteristics of the new circuit are clarified. And it is found that the maximum value exists in the 2nd output.

• Journal of the Korean Institute of Telematics and Electronics
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• v.20 no.4
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• pp.41-46
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• 1983

The current-fed DC-DC converter has only one energy storage reactor in series with the input for any number of outputs and is insensitive to transformer volt-second unbalance. It is considered that these properties of the converter are considerable advantages over other maltiple-output circuits. The steady-state and dynamic characteristic and stability for the current-fed DC-DC con-verter are analyzed in detail. The analysis is carried out by the state-space averaging method for the operation with the duty ratio less than 50% and is confirmed by the experiment. From the evaluation of stability it is identified that the stability of this converter is excellent as compared with that of the conventional buck type converter.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.38 no.6
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• pp.451-457
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• 1989

The current-fed DC-DC converter, which is known as the most stable DC-DC converter, has a two-winding reactor in series with the input. In this paper a new double-output DC-DC converter circuit, in which the 2nd winding of the reactor is creating the 2nd output, while the 2nd winding is feeding the energy to the input in the current-fed converter, is propose. The steady state characteristics of the new circuit are clarified and it is found that the maximum value exists in the 2nd output. Furthermore, regulation characteristic is analysed by 'Slope method' and the result shows good agreement with experimental value. The 2nd output voltage regulation is performed by using regulation IC. As a result, we have achieved good regulation characteristics.

• Journal of Microbiology and Biotechnology
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• v.8 no.3
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• pp.203-207
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• 1998

Fed-batch fermentations with different feeding media were carried out in order to increase the productivity of invertase expression using a recombinant Saccharomyces cerevisiae containing plasmid pRB58. Two batch cultures showed the expression of the SUC2 gene at a low concentration of glucose, suggesting that glucose concentration could be used as a control variable in a fed-batch operation mode. In the fed-batch culture by feeding the basal medium, cell mass and specific invertase activity did not increase much as compared with the simple batch culture. A series of fed-batch cultures revealed that the sucrose-supplemented medium increased cell mass whereas the enriched medium did specific invertase activity. To capitalize on the synergism of the sucrose-supplemented medium and the enriched medium, the sucrose-supplemented enriched medium was used as a feeding medium. The fed-batch culture using this medium resulted in a 2.4-fold increase in cell mass and a 1.9-fold enhancement in specific invertase activity compared with those of the batch culture. The increase in cell mass and specific invertase activity led to a marked increase in total invertase activity, 250U/ml, which was 6.3 times higher than that of the batch culture.

• ALGAE
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• v.36 no.1
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• pp.51-60
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• 2021

The sea urchin, Mesocentrotus nudus, is widely distributed in North West Pacific regions. It has a substantial impact on macroalgal communities as a generalist herbivore. This study examined various aspects of its feeding ecology, including algal preference, foraging behaviors, and possible effects of past feeding history on its algal preference. We used six common algal species (Ulva australis, Undaria pinnatifida, Sargassum confusum, Dictyopteris divaricata, Grateloupia elliptica, and Grateloupia angusta) from the east coast of Korea as food choice in a series of indoor aquarium experiments. The first choice of starved M. nudus was exclusively U. pinnatifida, followed by G. elliptica and S. confusum. Unlike large urchins, small urchins equally preferred U. pinnatifida and G. elliptica. On the other hand, Undaria-fed urchins preferred to feed only G. elliptica, although its preference slightly differed over time. We then grouped sea urchins into three categories (starved, Undaria-fed, mixed species-fed) to observe 12-days feeding preference as well as early foraging movements. Foraging behaviors of the three groups were distinctively different, although they could not completely reflect the actual consumption. For example, U. australis was highly attractive, but rarely eaten. Undaria-fed urchins seemed to stay with only S. confusum and U. australis. This study demonstrates that M. nudus shows high flexibility in food preference depending on past feeding history and body size. Its foraging behaviors are also affected by past feeding conditions, exhibiting active chemoreceptive movements.

• Proceedings of the KIPE Conference
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• 2006.06a
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• pp.277-280
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• 2006

Recently, the high frequency isolated boost DC/DC converter has been widely used for the PCS (Power Conditioning System) system because of its small size and low cost. However, the high frequency isolated boost DC/DC converters applied the conventional voltage-fed converter and current-fed converter have the problems such as the high conduction losses and the surge voltage due to the high circulating current and the leakage inductance, respectively. To overcome this problems, in this paper the secondary LLC resonant converter is proposed, and the experimental results of the secondary LLC series resonant converter for boost DC/DC converter are verified on the simulation based on the theoretical analysis and the 1kW experimental prototype.

• Bulletin of the Korean Chemical Society
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• v.29 no.4
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• pp.822-826
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• 2008

We report the cathodoluminescence and aging properties of a series of green phosphors of formula $Sr_{1-x}M_xGa_2S_4$:Eu (x = 0.0-1.0, M = Ba or Ca) that have potential applications in field emission displays (FEDs). The series of phosphors was synthesized via NaBr-aided solid-state reactions in a flowing $H_2S$ stream. A low level ($\sim$20%) of Ba or Ca substitution for Sr in $SrGa_2S_4$:Eu maintains the orthorhombic phase of pure $SrGa_2S_4$:Eu phosphors. Further, a low level ($\sim$20%) of Ba or Ca substitution for Sr in $SrGa_2S_4$:Eu provides various green colors and sufficient brightness for FED applications. Substitution of Ba or Ca for Sr in $SrGa_2S_4$:Eu also improved the stability of the phosphor when it was operated under electron-beam irradiation of 5 kV.