• Title/Summary/Keyword: Sequential Optimization

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Statistical Optimization of Solid Growth-medium for Rapid and Large Screening of Polysaccharides High-yielding Mycelial Cells of Inonotus obliquus (단백다당체 고생산성의 Inonotus obliquus 균주의 신속 개량을 위한 고체 성장배지의 통계적 최적화)

  • Hong, Hyung-Pyo;Jeong, Yong-Seob;Chun, Gie-Taek
    • KSBB Journal
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    • v.25 no.2
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    • pp.142-154
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    • 2010
  • The protein-bound innerpolysaccharides (IPS) produced by suspended mycelial cultures of Inonotus obliquus have promising potentials as an effective antidiabetic as well as an immunostimulating agents. To enhance IPS production, intensive strain improvement process should be carried out using large amount of UV-mutated protoplasts. During the whole strain-screening process, the stage of solid growth-culture was found to be the most time-requiring step, thus preventing rapid screening of high-yielding producers. In order to reduce the cell growth period in the solid growth-stage, therefore, solid growth-medium was optimized using the statistical methods such as (i) Plackett-Burman and fractional factorial designs (FFD) for selecting positive medium components, and (ii) steepest ascent (SAM) and response surface (RSM) methods for determining optimum concentrations of the selected components. By adopting the medium composition recommended by the SAM experiment, significantly higher growth rate was obtained in the solid growth-cultures, as represented by about 41% larger diameter of the cell growth circle and higher mycelial density. Sequential optimization process performed using the RSM experiments finally recommended the medium composition as follows: glucose 25.61g/L, brown rice 12.53 g/L, soytone peptone 12.53 g/L, $MgSO_4$ 5.53 g/L, and agar 20 g/L. It should be noted that this composition was almost similar to the medium combinations determined by the SAM experiment, demonstrating that the SAM was very helpful in finding out the final optimum concentrations. Through the use of this optimized medium, the period for the solid growth-culture could be successfully reduced to about 8 days from the previous 15~20 days, thus enabling large and mass screening of high producers in a relatively short period.

Operating Optimization and Economic Evaluation of Multicomponent Gas Separation Process using Pressure Swing Adsorption and Membrane Process (압력 순환 흡착과 막 분리공정을 이용한 다성분 기체의 분리공정 조업 최적화 및 경제성 평가)

  • Kim, Hansol;Lee, Jaewook;Lee, Soobin;Han, Jeehoon;Lee, In-Beum
    • Korean Chemical Engineering Research
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    • v.53 no.1
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    • pp.31-38
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    • 2015
  • At present, carbon dioxide ($CO_2$) emission, which causes global warming, is a major issue all over the world. To reduce $CO_2$ emission directly, commercial deployment of $CO_2$ separation processes has been attempted in industrial plants, such as power plant, oil refinery and steelmaking plant. Besides, several studies have been done on indirect reduction of $CO_2$ emission from recycle of reducing gas (carbon monoxide or hydrogen containing gas) in the plants. Unlike many competing gas separation technologies, pressure swing adsorption (PSA) and membrane filtration are commercially used together or individually to separate a single component from the gas mixture. However, there are few studies on operation of sequential separation process of multi-component gas which has more than two target gas products. In this paper, process simulation model is first developed for two available configurations: $CO_2$ PSA-CO PSA-$H_2$ PSA and $CO_2$ PSA-CO PSA-$H_2$ membrane. Operation optimization and economic evaluation of the processes are also performed. As a result, feed gas contains about 14% of $H_2$ should be used as fuel than separating $H_2$, and $CO_2$ separation should be separated earlier than CO separation when feed gas contains about 30% of $CO_2$ and CO. The simulation results can help us to find an optimal process configuration and operation condition for separation of multicomponent gas with $CO_2$, CO, $H_2$ and other gases.

Characterization and Culture Optimization of an Glucosidase Inhibitor-producing Bacteria, Gluconobactor oxydans CK-2165 (α-Glucosidase 저해제 생산 균주, Gluconobacter oxydans CK-2165의 특성 및 배양 최적화)

  • Kim, Byoung-Kook;Suh, Min-Jung;Park, Ji-Su;Park, Jang-Woo;Suh, Jung-Woo;Kim, Jin-Yong;Lee, Sun-Young;Choi, Jongkeun;Suh, Joo-Won;Lee, In-Ae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.11
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    • pp.5179-5186
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    • 2012
  • Miglitol, a well-known therapeutic intervention agents for diabetes, exhibits competitive inhibitory activity against ${\alpha}$-glucosidase and it is usually produced through three sequential steps including chemical and bioconversion processes. Gluconobactor oxydans (G. oxydans) belonging to acetic acid bacteria biologically, converts 1-deoxy-1-(2-hydroxyethylamino)-D-glucitol (P1) into a key intermidiate, 6-(2-hydroxyetyl) amino-6-deoxy-${\alpha}$-L-sorbofuranose (P2) by incomplete oxidation. In this study, we identified and optimized fermentation conditions of CK-2165, that was selected in soil samples by comparing the bioconversion yield. CK-2165 strain was found to be closely related to G. oxydans based on the result of phylogenetic analysis using 16S rDNA sequence. Utilization of API 20 kits revealed that this strain could use glucose, mannose, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin and arabinose as carbon sources. The culture conditions were optimized for industrial production and several important factors affecting bioconversion rate were also tested using mycelial cake. Cell harvested at the late-stationary phase showed the highest bioconversion yield and $MgSO_4$ was critically required for the catalytic activity.

Flow Calibration and Validation of Daechung Lake Watershed, Korea Using SWAT-CUP (SWAT-CUP을 이용한 대청호 유역 장기 유출 유량 보정 및 검증)

  • Lee, Eun-Hyoung;Seo, Dong-Il
    • Journal of Korea Water Resources Association
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    • v.44 no.9
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    • pp.711-720
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    • 2011
  • SWAT (Soil and Water Assessment Tool) model was calibrated for the flow rate of the Deachung lake with a large area of 3108.29 $km^2$. Application of SWAT model requires significant number of input data and is prone to result in uncertainties due to errors in input data, model structure and model parameters. The SUFI-2 (Sequential Uncertainty Fitting Ver. 2) program and GLUE (Generalized Likelihood Uncertainty Estimation) program in SWAT-CUP (SWAT-Calibration and Uncertainty Program) are used to select the best parameters for SWAT model. Optimal combination of parameter values was determined through 2,000 iterative SWAT model runs. The Nash-Sutcliffe values and $R^2$ values were 0.87 and 0.89 respectively indicating both methods show good agreements with observed data successfully. RMSE and MSE values also showed similar results for both programs. It seems the SWAT-CUP has a great practical appeal for parameter optimization especially for large basin area and it also can be used for less experienced SWAT model users.

Selective Ni Recovery from Spent Ni-Mo-Based Catalysts (니켈-몰리브데늄 성분계 폐촉매로부터 니켈의 선택적 회수)

  • Lee, Tae Kyo;Han, Gi Bo;Yoon, Suk Hoon;Lee, Tae Jin;Park, No-Kuk;Chang, Won Chul
    • Applied Chemistry for Engineering
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    • v.19 no.6
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    • pp.668-673
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    • 2008
  • The objective of this study is to optimize the leaching conditions of sequential leaching and extracting processes for selective Ni recovery from spent Ni-Mo-based catalyst. The selective Ni recovery process consists of two processes of leaching and extracting process. In this 2-step process, Ni component is dissolved from solid spent Ni-Mo-based catalyst into leaching agent in leaching process and sequentially extracted to Ni complex with an extracting agent in the extracting process. The solutions of nitric acid ($HNO_3$), ammonium carbonate ($(NH_4)_2CO_3$) and sodium carbonate ($Na_2CO_3$) were used as a leaching agent in leaching process and oxalic acid was used as an extracting agent in extracting process. $HNO_3$ solution is the most efficient leaching agent among the various leaching agent. Also, the optimized leaching conditions for the efficient and selective Ni recovery were the leaching temperature of $90^{\circ}C,\;HNO_3$ concentration of 6.25 vol% and elapsed time of 3 h. As a result, Nickel oxalate having the highest yield of 88.7% and purity of 100% was obtained after sequentially leaching and extracting processes under the optimized leaching conditions.

An Efficient Constraint Boundary Sampling Method for Sequential RBDO Using Kriging Surrogate Model (크리깅 대체모델을 이용한 순차적 신뢰성기반 최적설계를 위한 효율적인 제한조건경계 샘플링 기법)

  • Kim, Jihoon;Jang, Junyong;Kim, Shinyu;Lee, Tae Hee;Cho, Su-gil;Kim, Hyung Woo;Hong, Sup
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.6
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    • pp.587-593
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    • 2016
  • Reliability-based design optimization (RBDO) requires a high computational cost owing to its reliability analysis. A surrogate model is introduced to reduce the computational cost in RBDO. The accuracy of the reliability depends on the accuracy of the surrogate model of constraint boundaries in the surrogated-model-based RBDO. In earlier researches, constraint boundary sampling (CBS) was proposed to approximate accurately the boundaries of constraints by locating sample points on the boundaries of constraints. However, because CBS uses sample points on all constraint boundaries, it creates superfluous sample points. In this paper, efficient constraint boundary sampling (ECBS) is proposed to enhance the efficiency of CBS. ECBS uses the statistical information of a kriging surrogate model to locate sample points on or near the RBDO solution. The efficiency of ECBS is verified by mathematical examples.

Reviews of Bus Transit Route Network Design Problem (버스 노선망 설계 문제(BTRNDP)의 고찰)

  • Han, Jong-Hak;Lee, Seung-Jae;Lim, Seong-Su;Kim, Jong-Hyung
    • Journal of Korean Society of Transportation
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    • v.23 no.3 s.81
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    • pp.35-47
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    • 2005
  • This paper is to review a literature concerning Bus Transit Route Network Design(BTRNDP), to describe a future study direction for a systematic application for the BTRNDP. Since a bus transit uses a fixed route, schedule, stop, therefore an approach methodology is different from that of auto network design problem. An approach methodology for BTRNDP is classified by 8 categories: manual & guideline, market analysis, system analytic model. heuristic model. hybrid model. experienced-based model. simulation-based model. mathematical optimization model. In most previous BTRNDP, objective function is to minimize user and operator costs, and constraints on the total operator cost, fleet size and service frequency are common to several previous approach. Transit trip assignment mostly use multi-path trip assignment. Since the search for optimal solution from a large search space of BTRNDP made up by all possible solutions, the mixed combinatorial problem are usually NP-hard. Therefore, previous researches for the BTRNDP use a sequential design process, which is composed of several design steps as follows: the generation of a candidate route set, the route analysis and evaluation process, the selection process of a optimal route set Future study will focus on a development of detailed OD trip table based on bus stop, systematic transit route network evaluation model. updated transit trip assignment technique and advanced solution search algorithm for BTRNDP.

Bioleaching of Heavy Metals from Shooting Range Soil Using a Sulfur-Oxidizing Bacteria Acidithiobacillus thiooxidans (황산화균 Acidithiobacillus thiooxidans를 이용한 사격장 토양 내 중금속 용출)

  • Han, Hyeop-Jo;Lee, Jong-Un;Ko, Myoung-Soo;Choi, Nag-Choul;Kwon, Young-Ho;Kim, Byeong-Kyu;Chon, Hyo-Taek
    • Economic and Environmental Geology
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    • v.42 no.5
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    • pp.457-469
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    • 2009
  • Applicability of bioleaching techniques using a sulfur-oxidizing bacteria, Acidithiobacillus thiooxidans, for remediation of shooting range soil contaminated with toxic heavy metals was investigated. The effects of sulfur concentration, the amount of bacterial inoculum and operation temperature on the efficiency of heavy metal solubilization were examined as well. As sulfur concentration and the amount of bacterial inoculum increased, the solubilization efficiency slightly increased; however, significant decrease of heavy metal extraction was observed with no addition of sulfur or bacterial inoculum. Bacteria solubilized the higher amount of heavy metals at $26^{\circ}C$ than $4^{\circ}C$. Lead showed the highest removal amount from the contaminated soil but the lowest removal efficiency when compared with Zn, Cu and Cr. It was likely due to formation of insoluble $PbSO_{4(s)}$ as precipitate or colloidal suspension. Sequential extraction of the microbially treated soil revealed that the proportion of readily extractable phases of Zn, Cu and Cr increased by bacterial leaching, and thus additional treatment or optimization of operation conditions such as leaching time were required for safe reuse of the soil. Bioleaching appeared to be a useful strategy for remediation of shooting range soil contaminated with heavy metals, and various operating conditions including concentration of sulfur input, inoculum volume of bacteria, and operation temperature exerted significant influence on bioleaching efficiency.

Predicting blast-induced ground vibrations at limestone quarry from artificial neural network optimized by randomized and grid search cross-validation, and comparative analyses with blast vibration predictor models

  • Salman Ihsan;Shahab Saqib;Hafiz Muhammad Awais Rashid;Fawad S. Niazi;Mohsin Usman Qureshi
    • Geomechanics and Engineering
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    • v.35 no.2
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    • pp.121-133
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    • 2023
  • The demand for cement and limestone crushed materials has increased many folds due to the tremendous increase in construction activities in Pakistan during the past few decades. The number of cement production industries has increased correspondingly, and so the rock-blasting operations at the limestone quarry sites. However, the safety procedures warranted at these sites for the blast-induced ground vibrations (BIGV) have not been adequately developed and/or implemented. Proper prediction and monitoring of BIGV are necessary to ensure the safety of structures in the vicinity of these quarry sites. In this paper, an attempt has been made to predict BIGV using artificial neural network (ANN) at three selected limestone quarries of Pakistan. The ANN has been developed in Python using Keras with sequential model and dense layers. The hyper parameters and neurons in each of the activation layers has been optimized using randomized and grid search method. The input parameters for the model include distance, a maximum charge per delay (MCPD), depth of hole, burden, spacing, and number of blast holes, whereas, peak particle velocity (PPV) is taken as the only output parameter. A total of 110 blast vibrations datasets were recorded from three different limestone quarries. The dataset has been divided into 85% for neural network training, and 15% for testing of the network. A five-layer ANN is trained with Rectified Linear Unit (ReLU) activation function, Adam optimization algorithm with a learning rate of 0.001, and batch size of 32 with the topology of 6-32-32-256-1. The blast datasets were utilized to compare the performance of ANN, multivariate regression analysis (MVRA), and empirical predictors. The performance was evaluated using the coefficient of determination (R2), mean absolute error (MAE), mean squared error (MSE), mean absolute percentage error (MAPE), and root mean squared error (RMSE)for predicted and measured PPV. To determine the relative influence of each parameter on the PPV, sensitivity analyses were performed for all input parameters. The analyses reveal that ANN performs superior than MVRA and other empirical predictors, andthat83% PPV is affected by distance and MCPD while hole depth, number of blast holes, burden and spacing contribute for the remaining 17%. This research provides valuable insights into improving safety measures and ensuring the structural integrity of buildings near limestone quarry sites.

Korean Sentence Generation Using Phoneme-Level LSTM Language Model (한국어 음소 단위 LSTM 언어모델을 이용한 문장 생성)

  • Ahn, SungMahn;Chung, Yeojin;Lee, Jaejoon;Yang, Jiheon
    • Journal of Intelligence and Information Systems
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    • v.23 no.2
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    • pp.71-88
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    • 2017
  • Language models were originally developed for speech recognition and language processing. Using a set of example sentences, a language model predicts the next word or character based on sequential input data. N-gram models have been widely used but this model cannot model the correlation between the input units efficiently since it is a probabilistic model which are based on the frequency of each unit in the training set. Recently, as the deep learning algorithm has been developed, a recurrent neural network (RNN) model and a long short-term memory (LSTM) model have been widely used for the neural language model (Ahn, 2016; Kim et al., 2016; Lee et al., 2016). These models can reflect dependency between the objects that are entered sequentially into the model (Gers and Schmidhuber, 2001; Mikolov et al., 2010; Sundermeyer et al., 2012). In order to learning the neural language model, texts need to be decomposed into words or morphemes. Since, however, a training set of sentences includes a huge number of words or morphemes in general, the size of dictionary is very large and so it increases model complexity. In addition, word-level or morpheme-level models are able to generate vocabularies only which are contained in the training set. Furthermore, with highly morphological languages such as Turkish, Hungarian, Russian, Finnish or Korean, morpheme analyzers have more chance to cause errors in decomposition process (Lankinen et al., 2016). Therefore, this paper proposes a phoneme-level language model for Korean language based on LSTM models. A phoneme such as a vowel or a consonant is the smallest unit that comprises Korean texts. We construct the language model using three or four LSTM layers. Each model was trained using Stochastic Gradient Algorithm and more advanced optimization algorithms such as Adagrad, RMSprop, Adadelta, Adam, Adamax, and Nadam. Simulation study was done with Old Testament texts using a deep learning package Keras based the Theano. After pre-processing the texts, the dataset included 74 of unique characters including vowels, consonants, and punctuation marks. Then we constructed an input vector with 20 consecutive characters and an output with a following 21st character. Finally, total 1,023,411 sets of input-output vectors were included in the dataset and we divided them into training, validation, testsets with proportion 70:15:15. All the simulation were conducted on a system equipped with an Intel Xeon CPU (16 cores) and a NVIDIA GeForce GTX 1080 GPU. We compared the loss function evaluated for the validation set, the perplexity evaluated for the test set, and the time to be taken for training each model. As a result, all the optimization algorithms but the stochastic gradient algorithm showed similar validation loss and perplexity, which are clearly superior to those of the stochastic gradient algorithm. The stochastic gradient algorithm took the longest time to be trained for both 3- and 4-LSTM models. On average, the 4-LSTM layer model took 69% longer training time than the 3-LSTM layer model. However, the validation loss and perplexity were not improved significantly or became even worse for specific conditions. On the other hand, when comparing the automatically generated sentences, the 4-LSTM layer model tended to generate the sentences which are closer to the natural language than the 3-LSTM model. Although there were slight differences in the completeness of the generated sentences between the models, the sentence generation performance was quite satisfactory in any simulation conditions: they generated only legitimate Korean letters and the use of postposition and the conjugation of verbs were almost perfect in the sense of grammar. The results of this study are expected to be widely used for the processing of Korean language in the field of language processing and speech recognition, which are the basis of artificial intelligence systems.