• Journal of Ginseng Research
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• v.44 no.6
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• pp.775-783
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• 2020

Background: The reports about valuable oligosaccharides in ginseng are quite limited. There is an urgent need to develop a practical procedure to detect and analyze ginseng oligosaccharides. Methods: The oligosaccharide extracts from ginseng were permethylated by solid-phase methylation method and then were analyzed by ultra-high-performance liquid chromatography-Q-Orbitrap/MS. The sequence, linkage, and configuration information of oligosaccharides were determined by using accurate m/z value and tandem mass information. Several standard references were used to further confirm the identification. The oligosaccharide composition in white ginseng and red ginseng was compared using a multivariate statistical analysis method. Results: The nonreducing oligosaccharide erlose among 12 oligosaccharides identified was reported for the first time in ginseng. In the comparison of the oligosaccharide extracts from white ginseng and red ginseng, a clear separation was observed in the partial least squares-discriminate analysis score plot, indicating the sugar differences in these two kinds of ginseng samples. The glycans with variable importance in the projection value large than 1.0 were considered to contribute most to the classification. The contents of oligosaccharides in red ginseng were lower than those in white ginseng, and the contents of maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, sucrose, and erlose decreased significantly (p < 0.05) in red ginseng. Conclusion: A solid-phase methylation method combined with liquid chromatography-tandem mass spectrometry was successfully applied to analyze the oligosaccharides in ginseng extracts, which provides the possibility for holistic evaluation of ginseng oligosaccharides. The comparison of oligosaccharide composition of white ginseng and red ginseng could help understand the differences in pharmacological activities between these two kinds of ginseng samples from the perspective of glycans.

• The Journal of the Acoustical Society of Korea
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• v.22 no.2
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• pp.145-152
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• 2003

In this paper, assuming that the score of speech utterance is the product of HMM log likelihood and HMM weight, we propose a new method that HMM weights are adapted iteratively like the general MCE training. The proposed method adjusts HMM weights for better performance using delta coefficient defined in terms of misclassification measure. Therefore, the parameter estimation and the Viterbi algorithms of conventional 1:.um can be easily applied to the proposed model by constraining the sum of HMM weights to the number of HMMs in an HMM set. Comparing with the general segmental MCE training approach, computing time decreases by reducing the number of parameters to estimate and avoiding gradient calculation through the optimal state sequence. To evaluate the performance of HMM-based speech recognizer by weighting HMM likelihood, we perform Korean isolated digit recognition experiments. The experimental results show better performance than the MCE algorithm with state weighting.

• Journal of The Korean Association For Science Education
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• v.1 no.1
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• pp.15-27
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• 1978

First: The common points from "syllabus" period to that of "Course of study" are as follows: 1) with no introduction explained. the "Syllabus" or "Course of study" was made to be completed in accordance with the allotment of time (unit). 2) To teach how to rear animals and grow plants, and to make specimens with collected samples formed a great significant field of learning, which meant giving more emphasis on learning classification, life-centered education and basic field of learning than discipline-centered education. 3) The reason why the field of applied biology was emphasized on was that both periods had ideals in Common to educate persons more necessary and useful to the society than to major the pure academic field. 4) Both periods mainly dealt with problems of diseases, and physical health discussed all over the world in 1950's which accounts for necessity of the society to free from ignorance. Second; "The first curriculum period" and "the second one" are observed as follows: 1) The former took the unit (credit) system for the first time. It tried to lay down the conceptual hierarchy with "Biology I" and "II" divided, while "Biolgy I" is better systematized than "Biology II". 2) Discipline-centered education and structure fo knowledge are put more emphasis on especially in "the 2nd curriculum period". 3) And also in this period are included serious problems such as urgency of pollution, importance of nature conservation, population due to the development of industry. 'Third; With the recent curriculum laid down, experiments and teaching contents of subjects are put in harmony with each other and accordingly the process of Inquiry is laid emphasis on. Fourth; It is necessary to set up conceptual sequence and scope effectively in the curriculum.

• Journal of Life Science
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• v.18 no.11
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• pp.1617-1624
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• 2008

Chitin and chitosan, which is deacetylated form of chitin, are one of the most abundant biomass on the earth. They showed various biological activities including antimicrobial activity, heavy metal chelating, immune system activation, and have very diverse applications in food, pharmaceutical, medicinal, and environmental industry. There have been reported many chitin/chitosan-hydrolyzing enzymes, their structures and genes from three domains, archaea, bacteria, and eukarya. Carbohydrate hydrolyzing enzymes are classified in CAZy (Carbohydrate Active Enzymes) database according to their amino acid sequence similarity. Interestingly, chitinases and chitosanases are classified in various glycosyl hydrolase(GH) families, GH2, GH5, GH7, GH8, GH18, GH19, GH20, GH46, GH48, GH73, GH75, GH80, GH84, and GH85. Here, we review characteristics and structures of chitin/chitosan hydrolyzing enzymes according to glycosyl hydrolase families in order to provide information about gene mining.

• Journal of Korea Society of Industrial Information Systems
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• v.13 no.4
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• pp.145-154
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• 2008

In this paper, we propose the pet robot control system using hand gesture recognition in image sequences acquired from a camera affixed to the pet robot. The proposed system consists of 4 steps; hand detection, feature extraction, gesture recognition and robot control. The hand region is first detected from the input images using the skin color model in HSI color space and connected component analysis. Next, the hand shape and motion features from the image sequences are extracted. Then we consider the hand shape for classification of meaning gestures. Thereafter the hand gesture is recognized by using HMMs (hidden markov models) which have the input as the quantized symbol sequence by the hand motion. Finally the pet robot is controlled by a order corresponding to the recognized hand gesture. We defined four commands of sit down, stand up, lie flat and shake hands for control of pet robot. And we show that user is able to control of pet robot through proposed system in the experiment.

• Korean Parent-Child Health Journal
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• v.1
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• pp.56-74
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• 1998

The language of the newborn, like that of adults, is one of gesture, posture, and expression(Lewis, 1980). Helping parents understand and respond to their newborn's cues will make caring for their baby more enjoyable and may well provide the foundation for a communicative bond that will last lifetime. Infant state provides a dynamic pattern reflecting the full behavioral repertoire of the healthy infant(Brazelton, 1973, 1984). States are organized in a predictable emporal sequence and provide a basic classification of conditions that occur over and over again(Wolff, 1987). They are recognized by characteristic behavioral patterns, physiological changes, and infants' level of responsiveness. Most inportantly, however, states provide caregivers a framework for observing and understanding infants' behavior. When parents know how to determine whether their infant is sleep, awake, or drowsy, and they know the implications, recognition of states has for both the infant's behavior and for their caregiving, then a lot of hings about taking care of a newborn become much easier and more rewarding. Most parents have the skills and desire to do what is best for their infant. The skills 7373parents bring to the interaction are: the ability to read their infant's cues: to stimulate the baby through touch, movement, talking, and looking at: and to respond in a contingent manner to the infant's signals. Among the crucial skills infants bring to the interaction are perceptual abilities: hearing and seeing, the capacity to look at another for a period of time, the ability to smile, be consoled, adapt their body to holding or movement, and be regular and predictable in responding. Research demonstrates that the absence of these skills by either partner adversely affects parent-infant interaction and later development. Observing early parent-infant interactions during the hospital stay is important in order to identify parent-infant pairs in need of continued monitoring(Barnard, et al., 1989).

• Journal of the Korea Safety Management & Science
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• v.16 no.3
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• pp.195-200
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• 2014

Occupational fatal injury rate per 10,000 population of Korea is still higher among the OECD member countries. To prevent fatal injuries, the causes of accidents including human error should be analyzed and then appropriate countermeasures should be established. There was an severe converter furnace accident resulting in five people death by chocking in 2013. Although the accident type of the furnace accident was suffocation, many safety problems were included before reaching the death of suffocation. If the safety problems are reviewed throughly, the alternative measures based on the review would be very useful in preventing similar accidents. In this study, we investigated the converter furnace accident by using human error analysis and accident scenario analysis. As a result, it was found that the accident was caused by some human errors, inappropriate task sequence and lack of control in coordinating work by several subordinating companies. From the review of this case, the followings are suggested: First, systematic human error analysis should be included in the investigation of fatal injury accidents. Second, multi man-machine accident scenario analyis is useful in most of coordinating work. Third, the more provision of information on system state will lessen human errors. Fourth, the coordinating control in safety should be performed in the work conducting by several different companies.

• Economic and Environmental Geology
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• v.11 no.2
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• pp.47-57
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• 1978

The study focused on the petrology and petrochemistry of the so called "Ganghwa syenitic rocks" which intruded into metasediment of basement in southeastern part of Ganghwa Island. The geologic sequence of the mapped area was shown in table 1, 10 model analyses and 7 chemical analyses on the rock samples taken from the Ganghwa syenitic rocks and Manisan granite have been used to discuss the nomenclature of the rocks and petrological relationship between rock types. The petrograpical and petrochemical features based on, the analyses are as follows: 1) Ganghwa syenitic rocks consist of Ganghwa alkali syenite and Ganghwa diorite porphyry which based on the classification of the subcommision on systematics of igneous of IGUS. Ganghwa diorite porphyry which occured as dike forms are intruded into Ganghwa alkali syenite. The rock forming minerals of Ganghwa alkali syenite are composed of perthite, plagioclase, quartz, hornblend and chlorite in major, and zircon, apatite, sericite and magnetite in minor. Ganghwa diorite porphyries consist of plagioclase, biotite, hornblend, orthoclase and chlorite, with, porphyritic texture. 2) In silica-oxides variation (Fig. 2) and AMF diagram (Fig_ 3), the Ganghwa alkali syenite is similar to the trend of Daly's average basalt-andesite-dacite-rhyolite than Skaergaard which shows the trend of the fractional crystallization of magma, and equivalent to the alkali rock series by Peacock. 3) The general trend of data points shift to plagioclase, and are superimposed on the alkali rich terminal part of the granodiorite province of SW Finland in normative Q-Kf-Pl(Fig. 4) and Or-Ab-An diagram respectively. The above-mentioned evidences suggested that the Ganghwa syenitic rocks are the differential products resulted by assimilation of intermediated magma and metasedment rock under relatively rapid cooling condition.

• Parasites, Hosts and Diseases
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• v.53 no.6
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• pp.719-724
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• 2015

Nematomorpha, horsehair or Gordian worms, include about 300 freshwater species in 22 genera (Gordiida) and 5 marine species in 1 marine genus (Nectonema). They are parasitic in arthropods during their juvenile stage. In the present study, the used gordian worm was found in the feces of a dog (5-month old, male) in July 2014. Following the worm analysis using light and scanning electron microscopes, the morphological classification was re-evaluated with molecular analysis. The worm was determined to be a male worm having a bi-lobed tail and had male gonads in cross sections. It was identified as Gordius sp. (Nematomorpha: Gordiidae) based on the characteristic morphologies of cross sections and areole on the cuticle. DNA analysis on 18S rRNA partial sequence arrangements was also carried out, and the gordiid worm was assumed to be close to the genus Gordius based on a phylogenic tree analysis.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.159-166
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• 2013

Oryzias latipes and Oryzias sinensis are indigenous species found in Japan, China, and other East Asian countries, including Korea. Based on morphological differences, the species have been classified distinctly. However, the range of morphological characters such as the number of gill rakers, vertebrae, and spots on the lateral body overlaps and is too vague for clear identification, so their classification based on their morphological characteristics remains uncertain. In this study, the mitochondrial cytochrome oxidase subunit I (COI) gene, which is used for DNA barcoding, was applied to clarify interspecific variation of O. latipes and O. sinensis. Intraspecific genetic diversity was calculated to identify correlations with geographic distributions. We studied two species collected from 55 locations in Korea. All individuals carried a 679-base pair gene without deletion or insertion. Between species, 525 base pairs of the gene were shared. The Kimura two parameter (K2P) distance of O. latipes and O. sinensis was 0.41% and 1.39%, respectively. Mean divergence within genera was 23.5%. Therefore, the species were clearly different. The distance between O. latipes and O. sinensis was 14.0%, which is the closest within genera. Interestingly O. latipes from the Japanese and Korean group represented 16.5% distant. These results were derived from geohistorical and anthropogenic environmental factors. The O. latipes haplotypes were joined in only one group, but O. sinensis was divided into two groups, one is found in the Han River and upper Geum River watershed; the other is found in the remaining South Korean watersheds. Further studies will address the causes for geographic speciation of O. sinensis haplotypes.