• Korean Circulation Journal
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• 제53권12호
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• pp.826-828
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• 2023

• 사상체질의학회지
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• 제25권4호
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• pp.432-441
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• 2013

Objectives Despite the treatment with antibiotics, patients with sepsis has a high mortality (80%) in the underlying disease group. The aim of this study was to report the improvement of septic condition of the cholangiocarcinoma patient after the treatment with Handayeolso-tang, Fel Tauri, and antibiotics. Methods We retrospectively reviewed the medical records. The patient's subjective symptoms such as chilling and abdominal pain were evaluated by NRS and the performance status was evaluated by ECOG. This case was literally compared with relevant published studies on prognosis of sepsis. Results Despite poor prognostic factor(MEDS score 18), the patient's symptoms such as fever, chilling, abdominal pain, and diarrhea and ECOG(Eastern Cooperative Oncology Group) improved. The patient was hemodynamically stabilized on 3rd day from the treatment, and her laboratory test results were normalized on 7th day. Conclusions A female patient of metastatic cholangiocarcinoma came to the hospital for cholangitis, later causing septic shock. Both her symptoms and laboratory tests showed significant improvement after the treatment of antibiotics, Handayeolso-tang and Fel Tauri. To our knowledge, this is the first case reporting the synergistic combination of Korean oriental medicine and Western medicine approaching to sepsis.

• Journal of Chest Surgery
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• 제40권11호
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• pp.782-785
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• 2007

Lemierre 증후군은 구인두 감염증에 의해 유발되며 이차적으로 내경정맥의 패혈성 혈전정맥염이 발생하고 원격전이성 감염증이 흔하다. Fusobacterium necrophorum이 가장 흔한 원인 균주이고, 항균제의 출현 이전에는 사망률이 매우 높은 흔한 질환이었다. 항균제의 출현과 구인두 감염증에 대한 광범위한 항균제의 사용으로 발병이 매우 드물어, 이제는 잊혀져 가는 질환이 되었으나, 여전히 높은 사망률을 보이고 있다. 합병증과 사망률을 줄이기 위해서 빠른 진단과 적절한 항생제 치료가 필수적이다. 패혈성 폐색전증을 동반한 Lemierre 증후군 1예를 보고한다.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1707-1716
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• 2019

The development of new antimicrobial agents is essential for the effective treatment of diseases such as sepsis. We previously developed a new short peptide, Pap12-6, using the 12 N-terminal residues of papiliocin, which showed potent and effective antimicrobial activity against multidrug-resistant Gram-negative bacteria. Here, we investigated the antimicrobial mechanism of Pap12-6 and a newly designed peptide, Pap12-7, in which the 12^th Trp residue of Pap12-6 was replaced with Val to develop a potent peptide with high bacterial selectivity and a different antibacterial mechanism. Both peptides showed high antimicrobial activity against Gram-negative bacteria, including multidrug-resistant Gram-negative bacteria. In addition, the two peptides showed similar anti-inflammatory activity against lipopolysaccharide-stimulated RAW 264.7 cells, but Pap12-7 showed very low toxicities against sheep red blood cells and mammalian cells compared to that showed by Pap12-6. A calcein dye leakage assay, membrane depolarization, and confocal microscopy observations revealed that the two peptides with one single amino acid change have different mechanisms of antibacterial action: Pap12-6 directly targets the bacterial cell membrane, whereas Pap12-7 appears to penetrate the bacterial cell membrane and exert its activities in the cell. The therapeutic efficacy of Pap12-7 was further examined in a mouse model of sepsis, which increased the survival rate of septic mice. For the first time, we showed that both peptides showed anti-septic activity by reducing the infiltration of neutrophils and the production of inflammatory factors. Overall, these results indicate Pap12-7 as a novel non-toxic peptide with potent antibacterial and anti-septic activities via penetrating the cell membrane.

• 대한수의학회지
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• 제57권2호
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• pp.97-104
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• 2017

Solanum lycopersicum, commonly known as tomato, is widely used in raw, cooked, or liquid forms because it contains nutritional compounds that are beneficial for human health, including carotenoids, lycopene, ascorbic acid, vitamins, and minerals. The tomato is perhaps the most widely studied fruit, especially with respect to its cardioprotective effects. In this study, we aimed to identify the anti-inflammatory mechanisms by which the tomato elicits its anti-inflammatory properties. We treated murine macrophage RAW 264.7 cells with a tomato ethanol extract and performed various biochemical assays including nitric oxide inhibition, cell viability, RNA extraction, expression of pro-inflammatory mediators and cytokines, and immunoblotting, as well we assessed cell survival rates. Our results have shown for the first time that a tomato ethanol extract treatment can suppress nitric oxide production in a dose-dependent manner without cytotoxicity. Moreover, it inhibits the expression of pro-inflammatory mediators and cytokines and elicits its anti-inflammatory effects via the nuclear factor kappa-light-chain-enhancer of activated B cells ($NF-{\kappa}B$) and mitogen-activated protein kinase (MAPK) pathways. In addition, administration of tomato syrup potently rescued mice from septic shock induced by lipopolysaccharide injection. Collectively, our results elucidate details regarding the anti-inflammatory mechanisms of tomato.

• 한국방재학회 논문집
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• 제10권6호
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• pp.185-191
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• 2010

본 연구에서는 합류식 하수도에서 발생하는 악취에 대해 연구하였다. 도심 지역 합류식 하수도와 합류식 하수도 주요 악취 발생 시설로 나누어 연구를 진행 하였다. 각 각에 대해 특징을 갖는 지역을 선정하여 모니터링을 실시하였다. 도심 지역 합류식 하수도의 경우 황화합물에 대한 실시간 모니터링과 더불어 복합악취 분석을 하였다. 도심 지역의 합류식 하수도 악취는 이동 유동 인구의 변화와 정화조 유출수에 영향을 받았다. 합류식 하수도 주요 악취 발생 시설의 경우 정화조 유출수의 영향이 큰 것으로 나타났다. 합류식 하수도의 주요 악취 유발 물질은 황화수소와 메틸머캅탄 등의 황화합물로판명되었다. 합류식 하수도의 악취 저감을 위해서는 정화조 유출수에 대한 개선 및 황화합물 발생 억제를 위한 대책이 필요할 것이라 판단된다.

• Tuberculosis and Respiratory Diseases
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• 제78권3호
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• pp.289-292
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• 2015

Lemierre syndrome (LS) is a septic thrombophlebitis of the internal jugular vein (IJV) following an oropharyngeal infection. LS is commonly caused by normal anaerobic flora and treated with appropriate antibiotics and anticoagulation therapy. Although the incidence of disease is very rare, 15% cases of LS are fatal even in the antibiotic era because of disseminated septic thromboemboli. We reported a case of extensive bilateral LS due to methicillin-resistant Staphylococcus epidermidis in a 63-year-old female with lung adenocarcinoma. Initial examination revealed a retropharyngeal abscess; hence, intravenous ceftriaxone and steroid were initiated empirically. However, pulmonary thromboembolism developed and methicillin-resistant S. epidermidis was identified in the bacterial culture. Despite intensive antibiotic and anticoagulation therapies, extensive septic thrombophlebitis involving the bilateral IJV and superior vena cava developed. Adjunctive catheter-directed thrombolysis and superior vena cava stenting were performed and the patient received antibiotic therapy for an additional 4 weeks, resulting in complete recovery.

• Tuberculosis and Respiratory Diseases
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• 제73권6호
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• pp.312-319
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• 2012

Background: Muscle wasting in sepsis is associated with increased proteolysis. Interleukin-15 (IL-15) has been characterized as an anabolic factor for skeletal muscles. Our study aims to investigate the role of IL-15 in sepsis-induced muscle atrophy and proteolysis. Methods: Mice were rendered septic either by cecal ligation and puncture or by intraperitoneal injection of lipopolysaccharide (LPS, 10 mg/kg i.p.). Expression of IL-15 mRNA and protein was determined by reverse transcriptase polymerase chain reaction and Western blot analysis in the control and septic limb muscles. C2C12 skeletal muscle cells were stimulated in vitro with either LPS or dexamethasone in the presence and absence of IL-15 and sampled at different time intervals (24, 48, or 72 hours). IL-15 ($10{\mu}g/kg$) was intraperitoneally administered 6 hours before sepsis induction and limb muscles were sampled after 24 hours of sepsis. Cathepsin L activity was determined to measure muscle proteolysis. Atrogin-1 and muscle-specific ring finger protein 1 (MuRF1) expressions in limb muscle protein lysates was analyzed. Results: IL-15 mRNA expression was significantly lower in the limb muscles of septic mice compared to that of controls. Cathepsin L activity in C2C12 cells was significantly lower in presence of IL-15, when compared to that observed with individual treatments of LPS or dexamethasone or tumor necrosis factor ${\alpha}$. Further, the limb muscles of mice pre-treated with IL-15 prior to sepsis induction showed a lower expression of atrogin-1 and MuRF1 than those not pre-treated. Conclusion: IL-15 may play a role in protection against sepsis-induced muscle wasting; thereby, serving as a potential therapeutic target for sepsis-induced skeletal muscle wasting and proteolysis.

• 대한토목학회논문집
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• 제2권1호
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• pp.69-80
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• 1982

본(本) 연구(硏究)는 분뇨처리(糞尿處理)의 효율화( 效率化) 방안(方案)으로 정화조(淨化槽)슬러지와 생분뇨(生糞尿)를 현존(現存) 소화식(消化式) 분뇨처리장(糞尿處理場)에서 혼합처리(混合處理)할 수 있는 방안(方案)을 검토(檢討)할 목적(目的)으로 분뇨(糞尿) : 정화조(淨化槽)슬러지의 혼합비율(混合比率)을 9 : 1(#1), 7 : 3(#2)으로 하여 처리도(處理度) 실험(實驗)을 수행(遂行)하였다. 실험결과(實驗結果) 유기물(有機物)의 제법효율(除法效率)은 9 : 1(#1)의 비율(比率)에서 BOD 80%, COD 80%, TS 63%, VS 76%이었으며 7 : 3(#2)의 비율(比率)에서는 BOD 87%, COD 82%, TS 66%, VS 78%로 정화조슬러지를 분뇨처리장에서 혼합처리(混合處理)할 수 있는 충분(充分)한 가능성(可能性)을 보여주었다.

• 한국환경농학회지
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• 제29권3호
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• pp.232-238
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• 2010

본 연구에서는 UST-AF 시스템에 전(前)응집 후(後)생물학적 처리 공정을 도입하여 질소와 인을 동시에 제거하고자 하였다. 연속공정은 유기물 및 SS의 제거 효율이 90%이상으로 균등화 효과와 질산화 효율이 높았다. 호기성 여과조에서 질산화 효율이 95% 이상이었으며, 반송을 통한 탈질화 효율은 80% 이상이었다. 하수원수에 화학적 처리 공정을 도입하여 총인을 90% 이상 제거하였으며, 후속공정의 생물반응에 영향을 주지 않은 alum 주입량은 40 mg/L이었다. 그러나 하수원수에 포함된 응집제에 의한 생물반응의 저해는 관찰되지 않았으나, 향후 장기간 운전하였을 경우 공정내 축적된 응집 슬러지의 영향에 대한 고찰이 필요할 것으로 예상된다.