• IMMUNE NETWORK
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• v.9 no.6
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• pp.243-247
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• 2009

In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity.

• Journal of Institute of Control, Robotics and Systems
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• v.16 no.3
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• pp.269-276
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• 2010

In this paper, we present the detection method of moving objects based on two background models. These background models support to understand multi layered environment belonged in images taken by shaking camera and each model is MBM(Multiple Background Model) and TMBM (Temporal Median Background Model). Because two background models are Pixel-based model, it must have noise by camera movement. Therefore correlation coefficient calculates the similarity between consecutive images and measures camera motion vector which indicates camera movement. For the calculation of correlation coefficient, we choose the selected region and searching area in the current and previous image respectively then we have a displacement vector by the correlation process. Every selected region must have its own displacement vector therefore the global maximum of a histogram of displacement vectors is the camera motion vector between consecutive images. The MBM classifies the intensity distribution of each pixel continuously related by camera motion vector to the multi clusters. However, MBM has weak sensitivity for temporal intensity variation thus we use TMBM to support the weakness of system. In the video-based experiment, we verify the presented algorithm needs around 49(ms) to generate two background models and detect moving objects.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.6
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• pp.79-84
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• 2015

In a distribution of digital image, there is a serious problem that is a distribution of the altered image by a forger. For the problem solution, this paper proposes a median filtering (MF) image forensic decision algorithm using a feature vector according to the pixel value's gradients. In the proposed algorithm, AR (Autoregressive) coefficients are computed from pixel value' gradients of original image then 1th~6th order coefficients to be six feature vector. And the reconstructed image is produced by the solution of Poisson's equation with the gradients. From the difference image between original and its reconstructed image, four feature vector (Average value, Max. value and the coordinate i,j of Max. value) is extracted. Subsequently, Two kinds of the feature vector combined to 10 Dim. feature vector that is used in the learning of a SVM (Support Vector Machine) classification for MF (Median Filtering) detector of the altered image. On the proposed algorithm of the median filtering detection, compare to MFR (Median Filter Residual) scheme that had the same 10 Dim. feature vectors, the performance is excellent at Unaltered, Averaging filtering ($3{\times}3$) and JPEG (QF=90) images, and less at Gaussian filtering ($3{\times}3$) image. However, in the measured performances of all items, AUC (Area Under Curve) by the sensitivity and 1-specificity is approached to 1. Thus, it is confirmed that the grade evaluation of the proposed algorithm is 'Excellent (A)'.

• 한국데이터정보과학회:학술대회논문집
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• 2005.04a
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• pp.155-163
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• 2005

This paper applies Support Vector Regression (SVR) to estimate and forecast nonlinear autoregressive integrated (ARI) model of the daily exchange rates of four currencies (Swiss Francs, Indian Rupees, South Korean Won and Philippines Pesos) against U.S. dollar. The forecasting abilities of SVR are compared with linear ARI model which is estimated by OLS. Sensitivity of SVR results are also examined to kernel type and other free parameters. Empirical findings are in favor of SVR. SVR method forecasts exchange rate level better than linear ARI model and also has superior ability in forecasting the exchange rates direction in short test phase but has similar performance with OLS when forecasting the turning points in long test phase.

• Journal of Power Electronics
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• v.14 no.5
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• pp.989-999
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• 2014

High-performance Current Source Inverter (CSI)-fed, variable speed alternating current drives are prepared for various industrial applications. CSI-fed Induction Motor (IM) drives are managed by using different control methods. Noteworthy methods include scalar Control (V/f), Input-Output Linearization (IOL) control, Field-Oriented Control (FOC), and Direct Torque Control (DTC). The objective of this work is to compare the dynamic performance of the aforementioned drive control methods for CSI-fed IM drives. The dynamic performance results of the proposed drives are individually analyzed through sensitivity tests. The tests selected for the comparison are step changes in the reference speed and torque of the motor drive. The operation and performance of different vector control methods are verified through simulations with MATLAB/Simulink and experimental results.

• The Journal of the Acoustical Society of Korea
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• v.31 no.8
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• pp.569-579
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• 2012

Typical Tonpilz type underwater acoustic transducers making use of piezoelectric ceramics detect the magnitude of an acoustic pressure, a scalar quantity, and convert this pressure into a proportional output voltage. The scalar sensor has no directional sensitivity. In this paper, we have proposed a new vector sensor based on the Tonpilz transducer structure, which is sensitive to both the magnitude and the azimuthal direction of an acoustic wave. Validity of this new design has been confirmed with analytic equations and finite element analyses.

• Ocean Systems Engineering
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• v.5 no.1
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• pp.41-54
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• 2015

This study presents the development of predictive models for uplift capacity of suction caisson in clay using an artificial intelligence technique, extreme learning machine (ELM). Other artificial intelligence models like artificial neural network (ANN), support vector machine (SVM), relevance vector machine (RVM) models are also developed to compare the ELM model with above models and available numerical models in terms of different statistical criteria. A ranking system is presented to evaluate present models in identifying the 'best' model. Sensitivity analyses are made to identify important inputs contributing to the developed models.

• Proceedings of the KIEE Conference
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• 2003.07d
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• pp.2161-2163
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• 2003

This Paper presents a vector control method for the parallel-connected motor drive system. The new estimation scheme of rotor flux position is presented to reduce sensitivity due to load difference between the motors. To confirm the validity of the proposed control method, we compare a simulation result of the proposed control method with that of the conventional indirect vector control method. The simulation results show that the proposed control method is more effective step change in load torque.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2007.04a
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• pp.577-582
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• 2007

A variational formulation for plane elasticity problems is derived based on an isogeometric approach. The isogeometric analysis is an emerging methodology such that the basis functions in analysis domain arc generated directly from NURBS (Non-Uniform Rational B-Splines) geometry. Thus. the solution space can be represented in terms of the same functions to represent the geometry. The coefficients of basis functions or the control variables play the role of degrees-of-freedom. Furthermore, due to h-. p-, and k-refinement schemes, the high order geometric features can be described exactly and easily without tedious re-meshing process. The isogeometric sensitivity analysis method enables us to analyze arbitrarily shaped structures without re-meshing. Also, it provides a precise construction method of finite element model to exactly represent geometry using B-spline base functions in CAD geometric modeling. To obtain precise shape sensitivity, the normal and curvature of boundary should be taken into account in the shape sensitivity expressions. However, in conventional finite element methods, the normal information is inaccurate and the curvature is generally missing due to the use of linear interpolation functions. A continuum-based adjoint sensitivity analysis method using the isogeometric approach is derived for the plane elasticity problems. The conventional shape optimization using the finite element method has some difficulties in the parameterization of boundary. In isogeometric analysis, however, the geometric properties arc already embedded in the B-spline shape functions and control points. The perturbation of control points in isogeometric analysis automatically results in shape changes. Using the conventional finite clement method, the inter-element continuity of the design space is not guaranteed so that the normal vector and curvature arc not accurate enough. On tile other hand, in isogeometric analysis, these values arc continuous over the whole design space so that accurate shape sensitivity can be obtained. Through numerical examples, the developed isogeometric sensitivity analysis method is verified to show excellent agreement with finite difference sensitivity.

• Water for future
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• v.21 no.4
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• pp.407-414
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• 1988

The purpose of this paper is to study the following ; (1) how the stability and sensitivity of a given stream water quality model can be analyzed theoretically by means of the stability theory and the sensitivity theory, and (2) point out that the results of this study prove that numerical analysis for the given stream water quality model is reliable, and the model is sensitive for the variations of parameters. A stability theory which is described by the infinite Fourier series is used to analyze the numerical scheme of the model. The numerical shheme is used a backward implicit scheme. a sensitivity theory which is described by the first order linear vector equation is used to analyze theoretically the effect of variations of water quality parameters such as BOD loads, flow rate, temperature. The results of sensitivity theory are of general applicability and are presented in a analytical form. The results of this study seems to be satisfactory for the reliability of stream water quality model with respect to the numerical scheme and the variations of the water quality parameters.